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- 저자 : MichaelJ.Mccarthy2 (검색결과 1 건)
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Investigation of Olive Accessions using Nuclear Magnetic Resonance
SeongMinKim,MichaelJ.Mccarthy2 전북대학교 농업과학기술연구소 2010 농업생명과학연구 Vol.41 No.1
In this study we used NMR to assess oil content in the 201 olive accessions in the Davis- National Clonal Germplasm Repository(NCGR) collection. Characterization of the olive collection will greatly facilitate stakeholder utilization of this important collection. Since oil content varies widely in olive accessions and oil production is the most important use of olives worldwide, characterization of oil content is critical data for the NCGR olive collection. Oil and moisture content were measured using a proton spectra and integrating this to obtain total signal intensity from the oil and moisture. Oil content is one of the most commercially important attributes of olive genotypes. NMR has been established as an accurate and rapid method for detecting oil content in intact olives. These data were collected at three dates, at monthly intervals, targeting the date of 90% color development for each accession. CPMG pulse sequence was used to acquire signal in time domain and T2 was calculated using a bi-exponential curve fitting method. Inversion recovery pulse sequence was used to acquire spectroscopic data and T1 were calculated for water and oil absorption from proton spectra. The relaxation time constants are useful indexes for examining the state of olive accessions.
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