Effects of Dietary Supplementation with Hainanmycin on Protein Degradation and Populations of Ammonia-producing Bacteria In vitro

Wang, Z.B.,Xin, H.S.,Wang, M.J.,Li, Z.Y.,Qu, Y.L.,Miao, S.J.,Zhang, Y.G. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.5

An in vitro fermentation was conducted to determine the effects of hainanmycin on protein degradation and populations of ammonia-producing bacteria. The substrates (DM basis) for in vitro fermentation consisted of alfalfa hay (31.7%), Chinese wild rye grass hay (28.3%), ground corn grain (24.5%), soybean meal (15.5%) with a forage: concentrate of 60:40. Treatments were the control (no additive) and hainanmycin supplemented at 0.1 (H0.1), 1 (H1), 10 (H10), and 100 mg/kg (H100) of the substrates. After 24 h of fermentation, the highest addition level of hainanmycin decreased total VFA concentration and increased the final pH. The high addition level of hainanmycin (H1, H10, and H100) reduced (p<0.05) branched-chain VFA concentration, the molar proportion of acetate and butyrate, and ratio of acetate to propionate; and increased the molar proportion of propionate, except that for H1 the in molar proportion of acetate and isobutyrate was not changed (p>0.05). After 24 h of fermentation, H10 and H100 increased (p<0.05) concentrations of peptide nitrogen and AA nitrogen and proteinase activity, and decreased (p<0.05) $NH_3$-N concentration and deaminase activity compared with control. Peptidase activitives were not affected by hainanmycin. Hainanmycin supplementation only inhibited the growth of Butyrivibrio fibrisolvens, which is one of the species of low deaminative activity. Hainanmycin supplementation also decreased (p<0.05) relative population sizes of hyper-ammonia-producing species, except for H0.1 on Clostridium aminophilum. It was concluded that dietary supplementation with hainanmycin could improve ruminal fermentation and modify protein degradation by changing population size of ammonia-producing bacteria in vitro; and the addition level of 10 mg/kg appeared to achieve the best results.

The Nutrition Requirements and Foraging Behaviour of Ostriches

Miao, Z.H.,Glatz, P.C.,Ru, Y.J. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.5

Ostrich farming is a developing industry in most countries in the world, with farm profitability being largely dependent on the quality of the products, especially skins and meat. To produce quality products, it is essential to ensure that nutrient supply matches the nutrient requirements of ostriches during their growth. To achieve this, information on feed utilisation efficiency and nutrient requirements of ostriches at different maturity stages is required. In South Africa, a number of experiments were carried out to assess the nutritive value of feed and to define the nutrient requirement of ostriches. These data were derived from limited number of birds and the direct application of the results to ostrich farming in Australia and other countries is questionable due to the difference in environment and feed resources. Initially ostrich farmers used data from poultry as a guideline for feed formulation, but in recent years more data has become available for ostriches. Ostriches have a better feed utilisation efficiency and a larger capacity of using high fibre feeds such as pastures than poultry. This review revealed that there are a number of areas there further nutritional research and development is required to ensure the ostriches are provided suitable diets to maximise farm profitability. These include the assessment of the nutritive value of feed ingredients for ostrich chicks and adult birds, the determination of nutrient requirements of ostriches under different farming systems, the development of ostrich diet for producing specific product, and grazing management strategies of ostriches in a crop-pasture rotation system.

Review of Production, Husbandry and Sustainability of Free-range Pig Production Systems

Miao, Z.H.,Glatz, P.C.,Ru, Y.J. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.11

A review was undertaken to obtain information on the sustainability of pig free-range production systems including the management, performance and health of pigs in the system. Modern outdoor rearing systems requires simple portable and flexible housing with low cost fencing. Local pig breeds and outdoor-adapted breeds for certain environment are generally more suitable for free-range systems. Free-range farms should be located in a low rainfall area and paddocks should be relatively flat, with light topsoil overlying free-draining subsoil with the absence of sharp stones that can cause foot damage. Huts or shelters are crucial for protecting pigs from direct sun burn and heat stress, especially when shade from trees and other facilities is not available. Pigs commonly graze on strip pastures and are rotated between paddocks. The zones of thermal comfort for the sow and piglet differ markedly; between 12-22$^{\circ}C$ for the sow and 30-37$^{\circ}C$ for piglets. Offering wallows for free-range pigs meets their behavioural requirements, and also overcomes the effects of high ambient temperatures on feed intake. Pigs can increase their evaporative heat loss via an increase in the proportion of wet skin by using a wallow, or through water drips and spray. Mud from wallows can also coat the skin of pigs, preventing sunburn. Under grazing conditions, it is difficult to control the fibre intake of pigs although a high energy, low fibre diet can be used. In some countries outdoor sows are fitted with nose rings to prevent them from uprooting the grass. This reduces nutrient leaching of the land due to less rooting. In general, free-range pigs have a higher mortality compared to intensively housed pigs. Many factors can contribute to the death of the piglet including crushing, disease, heat stress and poor nutrition. With successful management, free-range pigs can have similar production to door pigs, although the growth rate of the litters is affected by season. Piglets grow quicker indoors during the cold season compared to outdoor systems. Pigs reared outdoors show calmer behaviour. Aggressive interactions during feeding are lower compared to indoor pigs while outdoor sows are more active than indoor sows. Outdoor pigs have a higher parasite burden, which increases the nutrient requirement for maintenance and reduces their feed utilization efficiency. Parasite infections in free-range pigs also risks the image of free-range pork as a clean and safe product. Diseases can be controlled to a certain degree by grazing management. Frequent rotation is required although most farmers are keeping their pigs for a longer period before rotating. The concept of using pasture species to minimise nematode infections in grazing pigs looks promising. Plants that can be grown locally and used as part of the normal feeding regime are most likely to be acceptable to farmers, particularly organic farmers. However, one of the key concerns from the public for free-range pig production system is the impact on the environment. In the past, the pigs were held in the same paddock at a high stocking rate, which resulted in damage to the vegetation, nutrient loading in the soil, nitrate leaching and gas emission. To avoid this, outdoor pigs should be integrated in the cropping pasture system, the stock should be mobile and stocking rate related to the amount of feed given to the animals.

Free-range Poultry Production - A Review

Miao, Z.H.,Glatz, P.C.,Ru, Y.J. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1

With the demand for free-range products increasing and the pressure on the intensive poultry industry to improve poultry welfare especially in western countries, the number of free-range poultry farms has increased significantly. The USA, Australia and European countries have developed Codes of Practice for free-range poultry farming which detail the minimum standards of husbandry and welfare for birds. However, the performance and liveability of free-range birds needs to be improved and more knowledge is required on bird husbandry, feed supply, disease control and heat wave management. This review examines the husbandry, welfare, nutrition and disease issues associated with free-range poultry systems and discusses the potential of incorporating free-range poultry into a crop-pasture rotation system.

Recurrence after Anatomic Resection Versus Nonanatomic Resection for Hepatocellular Carcinoma: A Meta-analysis

Ye, J.Z.,Miao, Z.G.,Wu, F.X.,Zhao, Y.N.,Ye, H.H.,Li, L.Q. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The impact of anatomic resection (AR) as compared to non-anatomic resection (NAR) for hepatocellular carcinoma (HCC) as a factor for preventing intra-hepatic and local recurrence after the initial surgical procedure remains controversial. A systematic review and meta-analysis of nonrandomized trials comparing anatomic resection with non-anatomic resection for HCC published from 1990 to 2010 in PubMed and Medline, Cochrane Library, Embase, and Science Citation Index were therefore performed. Intra-hepatic recurrence, including early and late, and local recurrence were considered as primary outcomes. As secondary outcomes, 5 year survival and 5 year disease-free survival were considered. Pooled effects were calculated utilizing either fixed effects or random effects models. Eleven non-randomized studies including 1,576 patients were identified and analyzed, with 810 patients in the AR group and 766 in the NAR group. Patients in the AR group were characterized by lower prevalence of cirrhosis, more favorable hepatic function, and larger tumor size and higher prevalence of macrovascular invasion compared with patients in the NAR group. Anatomic resection significantly reduced the risks of local recurrence and achieved a better 5 years disease-free survival. Also, anatomic resection was marginally effective for decreasing the early intra-hepatic recurrence. However, it was not advantageous in preventing late intra-hepatic recurrence compared with non-anatomic resection. No differences were found between AR and NAR with respect to postoperative morbidity, mortality, and hospitalization. Anatomic resection can be recommended as superior to non-anatomic resection in terms of reducing the risks of local recurrence, early intra-hepatic recurrence and achieving a better 5 year disease-free survival in HCC patients.

Predicting Feed Intake of Fallow Deer (Dama Dama) Using Alkanes as a Marker

Ru, Y.J.,Miao, Z.H.,Glatz, P.C.,Choct, M. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.2

The understanding of seasonal forage intake of grazing deer is essential for the development of supplementary feeding strategies in southern Australia. The alkane technique is used in other animal species for estimating feed intake of individual animals and their diet composition. To assess the potential of using alkanes as a marker for predicting feed intake of fallow deer, the daily faecal recovery of alkanes and excretion rate of dosed artificial alkanes (C32 and C36) were measured with 6 deer fed three forage based diets. The artificial alkane capsule designed for use in sheep is suitable for fallow deer. Faecal samples need to be collected over days 7-19 after dosing. The daily excretion rate was 40 mg for C32 and 37 mg for C36. The faecal recovery of natural alkanes is incomplete and the faecal concentrations of alkanes need to be adjusted for an accurate estimation of intake. The actual feed intake of 6 experimental deer over a 5 day period was accurately estimated ($R^2$=0.52) using alkanes.

Comparison of the Digestibility of Grain and Forage by Sheep, Red and Fallow Deer

Ru, Y.J.,Glatz, P.C.,Miao, Z.H.,Swanson, K.,Falkenberg, S.,Wyatt, S. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.6

Two experiments were conducted to compare digestibility of 12 diets in sheep, red and fallow deer. No differences (p>0.05) between sheep, red and fallow deer in digestibility of dry matter, organic matter and digestible energy content for all diets were found except for the sorghum diet and medic hay. Sheep and fallow deer digested the sorghum diet better than red deer. An in vitro study showed that sheep had a lower in vitro dry matter digestibility and digestible energy content than both red and fallow deer, with a significant interaction between animal species and feed ingredient. Deer digested straws and hays better (p<0.05) than sheep. In vitro digestibility was lower (p<0.05) than in vivo digestibility, but significantly correlated with in vivo digestibility for red and fallow deer. The in vitro method for digestibility estimation has potential as a rapid feed evaluation system for deer, but needs further validation.

Impact of Salt Intake on Red and Fallow Deer Production in Australia - Review -

Ru, Y.J.,Glatz, P.C.,Miao, Z.H. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.12

Southern and south-western Australia is a typical mediterranean environment, characterised by wet, cold winters and dry, hot summers. The evaporation rate varies significantly in summer, resulting in a high salinity of drinking water for grazing animals. In addition, a large amount of land in the cropping areas is affected by salt. Puccinellia, tall wheat grass and saltbushes have been planted to improve the soil condition and to supply feed for grazing animals. Animals grazing these areas often ingest an excessive amount of salt from soil, forage and drinking water which can reduce feed intake, increase the water requirement, depress growth and affect body composition as demonstrated in sheep. While the deer industry has been successfully developed in these regions, the potential impact of excessive salt intake on deer production is unknown. The salt tolerance has been well defined for sheep, cattle and other livestock species, but the variation between animal species, breeds within species, maturity status and grazing environments makes it impossible to apply these values directly to deer. To optimise deer production and effectively use natural resources, it is essential to understand the salt status of grazing deer and the impact of excessive salt intake on growth and reproduction of deer.

Ablation of irradiated metals by high-intensity pulsed ion beam

X. P. Zhu,M. K. Lei,S. M. Miao,T. C. Ma,Z. H. Dong 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.III

Surface morphology and roughness of pure Ti irradiated by high-intensity pulsed ion beam (HIPIB) have been investigated by using scanning electronic microscope (SEM) and profilometer, respectively. The HIPIB irradiation was carried out at an accelerating voltage of 220 kV, an ion current density of \symbol{126}250 A/cm$^2$ with 75 ns pulse width under single or multi shots. Craters were formed on the irradiated surfaces and their dimension was expanded with increasing the ion current density. At $<$90 A/cm$% ^2 $, small craters of $\mu $m size were observed on the weakly melted surface. At 90-120 A/cm$^2$, large craters about 10 $\mu $m were found on the obviously melted surface. At 120-250 A/cm$^2$, the surface was severely melted with craters and the size of the craters increased to 20-80 $\mu $m with a wavy feature. At 250 A/cm$^2$ with 1 shot, surface roughness (\textit{% R}$_{\mathit{a}}$) greatly increased to a maximal value of 0.43 $\mu $m from the initial \textit{R}$_{\mathit{a}}$ of 0.18 $\mu $m, in good agreement with the SEM observation. With further increasing the shot number, the \textit{R}$_{\mathit{a}}$ decreased continuously and finally reached 0.06 $% \mu $m with 30 shots, indicating a planar ablated surface. The micro non-uniformity on the surfaces induced a selective ablation under HIPIB irradiation. The locally liquid evaporation and droplet ejection led to a disturbance in the molten surface layer to different scales depending on the ion current densities. On the other hand, the micro non-uniformity disappeared gradually under multi-shot irradiation, resulting in a more uniform ablation due to the diminished selective ablation.

The Influence of Weaning Time on Deer Performance

Bao, Y.M.,Ru, Y.J.,Glatz, P.C.,Miao, Z.H. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.4

Current growth in the deer industry is 20% per annum as the demand increases for venison in Europe, including the increased use in Asia of antlers as a natural medicine by humans. The deer industry in Australia has developed rapidly, but farm profitability has fluctuated markedly. The knowledge on deer farming has largely been adopted from New Zealand (NZ) and the United Kingdom (UK) although the environmental conditions in these deer growing countries differ markedly from Australia. The practice used for weaning is one of the key factors influencing the profitability of the industry. However, a wide range in weaning dates are practised by farmers leading to the question of whether deer should be weaned after mating to reduce the stress during weaning or should they be weaned in the pre-rut period to allow does to recover their body condition for the next reproduction cycle. This review examines the effect of weaning time on the performance of does and hinds and the subsequent growth rate of the fawns and calves. Gaps in knowledge of weaning procedures and nutritional management for early weaned deer are identified.