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One-step synthesis of gene carrier via gamma irradiation and its application in tumor gene therapy
Jeong, Sung In,Park, Seong-Cheol,Park, Sun-Jeong,Kim, Eun-Ji,Heo, Hun,Park, Jong-Seok,Gwon, Hui-Jeong,Lim, Youn-Mook,Jang, Mi-Kyeong DOVE MEDICAL PRESS 2018 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.13 No.-
<P><B>Introduction</B></P><P>Although numerous studies have been conducted with the aim of developing drug-delivery systems, chemically synthesized gene carriers have shown limited applications in the biomedical fields due to several problems, such as low-grafting yields, undesirable reactions, difficulties in controlling the reactions, and high-cost production owing to multi-step manufacturing processes.</P><P><B>Materials and methods</B></P><P>We developed a 1-step synthesis process to produce 2-aminoethyl methacrylate-grafted water-soluble chitosan (AEMA-g-WSC) as a gene carrier, using gamma irradiation for simultaneous synthesis and sterilization, but no catalysts or photoinitiators. We analyzed the AEMA graft site on WSC using 2-dimensional nuclear magnetic resonance spectroscopy (2D NMR; 1H and 13C NMR), and assayed gene transfection effects in vitro and in vivo.</P><P><B>Results</B></P><P>We revealed selective grafting of AEMA onto C6-OH groups of WSC. AEMA-g-WSC effectively condensed plasmid DNA to form polyplexes in the size range of 170 to 282 nm. AEMA-g-WSC polyplexes in combination with psi-hBCL2 (a vector expressing short hairpin RNA against <I>BCL2</I> mRNA) inhibited tumor cell proliferation and tumor growth in vitro and in vivo, respectively, by inducing apoptosis.</P><P><B>Conclusion</B></P><P>The simple grafting process mediated via gamma irradiation is a promising method for synthesizing gene carriers.</P>
이미정 ( Lee Mi Jeong ),송호경 ( Song Ho Gyeong ),이준우 ( Lee Jun U ),전권석 ( Jeon Gwon Seog ),김효정 ( Kim Hyo Jeong ),정도현 ( Jeong Do Hyeon ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.4
This study was carried out to select proper species for early stage replantation in forest road cut-slope. From one to fifteen year elapsed forest roads in four regional areas of Chungchung-do, sample plots were selected, and their vegetations and environmental factors were investigated. Invaded species with high frequency ordered Lespedeza bicolor, Rubus crataegifolius, Pueraria thunbergiana, Lysimachia clethroides, Pinus densiflora, Youngia denticulata, Patrinia villosa, and Spodiopogon sibiricus. North cut-slope have the highest value of invaded species number and plant coverage, and so, north aspect cuould be best for plant invasion. According to ordination, distribution of species was influenced by elapsed year and cut-slope aspect. Dactylis glomerata, Eragrostis curvula, Lespedeza bicolor, Lespedeza cuneate, Amorpha fruticosa, Patrinia scabiosaefolia, Artemisia princeps var. orienfalis, Miscanthus sinensis, and Pinus densiflora were considered as proper species for replantation at south slope. Lolium perenne, Dactylis glomerata, Eragrostis curvula, Lespedeza bicolor, Lespedeza cuneata, Amorpha fruticosa, Pueraria thunbergiana, Carex humitis, Patrinia scabiosaefolia, Artemisia princeps var. orientalis, Artemisia keiskeana, Clematis apiifolia, Zanthoxylum schinifolium, Rhus chinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, and Pinus densiflora were considered as proper species for replantation at north slope. Sowing herbs(Eragrostis curvula, Lolium perenne, Dactylis glomerata) that were seeded in early stage have changed into invasive herbs, parachute shrubs, and gravitative trees. Naturalized plants(Eragrostis curvula, Dactylis glomerata) that were seeded excessively in early stage have disturbed ecological succession in forest road cut-slope.
Rapid Micropropagation by Axillary Buds Cultures of Smilax china
Hyun-Jin Song,Seon-Jeong Sim,Mi-Jin Jeong,Chang-Mi Heo,Hak-Gon Kim,Gwon-Yong Jeong,Su-Yeoung Heo,Yong-Weon Choi,Geun-Hye Park,Jae-Kyung Yang,Hyun-Shik Moon,Myung-Suk Choi 경상대학교 농업생명과학연구원 2010 농업생명과학연구 Vol.44 No.6
An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, 0.5 mg L -1 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins α-napthalene acetic acid (NAA), indole acetic acid (IAA) and β-indolebutyric acid (IBA) (0.5 and 1.0 mg L -1 ). The 1.0 mg L -1 IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.