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      저자 : Meyer Thomas J. (검색결과 2 건)
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        A study of transposable element-associated structural variations (TASVs) using a de novo-assembled Korean genome

        Mun Seyoung,Kim Songmi,Lee Wooseok,Kang Keunsoo,Meyer Thomas J.,Han Bok-Ghee,Han Kyudong,김희수 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

        Advances in next-generation sequencing (NGS) technology have made personal genome sequencing possible, and indeed, many individual human genomes have now been sequenced. Comparisons of these individual genomes have revealed substantial genomic differences between human populations as well as between individuals from closely related ethnic groups. Transposable elements (TEs) are known to be one of the major sources of these variations and act through various mechanisms, including de novo insertion, insertion-mediated deletion, and TE–TE recombination-mediated deletion. In this study, we carried out de novo whole-genome sequencing of one Korean individual (KPGP9) via multiple insert-size libraries. The de novo whole-genome assembly resulted in 31,305 scaffolds with a scaffold N50 size of 13.23 Mb. Furthermore, through computational data analysis and experimental verification, we revealed that 182 TE-associated structural variation (TASV) insertions and 89 TASV deletions contributed 64,232 bp in sequence gain and 82,772 bp in sequence loss, respectively, in the KPGP9 genome relative to the hg19 reference genome. We also verified structural differences associated with TASVs by comparative analysis with TASVs in recent genomes (AK1 and TCGA genomes) and reported their details. Here, we constructed a new Korean de novo whole-genome assembly and provide the first study, to our knowledge, focused on the identification of TASVs in an individual Korean genome. Our findings again highlight the role of TEs as a major driver of structural variations in human individual genomes.

      • Stabilization of Ruthenium(II) Polypyridyl Chromophores on Nanoparticle Metal-Oxide Electrodes in Water by Hydrophobic PMMA Overlayers

        Wee, Kyung-Ryang,Brennaman, M. Kyle,Alibabaei, Leila,Farnum, Byron H.,Sherman, Benjamin,Lapides, Alexander M.,Meyer, Thomas J. American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.39

        <P>We describe a poly(methyl methacrylate) (PMMA) dip-coating procedure, which results in surface stabilization of phosphonate and carboxylate derivatives of Ru(II)-polypyridyl complexes surface-bound to mesoporous nanoparticle TiO<SUB>2</SUB> and nanoITO films in aqueous solutions. As shown by contact angle and transmission electron microscopy (TEM) measurements, PMMA oligomers conformally coat the metal-oxide nanoparticles changing the mesoporous films from hydrophilic to hydrophobic. The thickness of the PMMA overlayer on TiO<SUB>2</SUB>–Ru(II) can be controlled by changing the wt % of PMMA in the dipcoating solution. There are insignificant perturbations in electrochemical or spectral properties at thicknesses of up to 2.1 nm with the Ru(III/II) couple remaining electrochemically reversible and <I>E</I><SUB>1/2</SUB> values and current densities nearly unaffected. Surface binding by PMMA overlayers results in stable surface binding even at pH 12 with up to a ∼100-fold enhancement in photostability. As shown by transient absorption measurements, the MLCT excited state(s) of phosphonate derivatized [Ru(bpy)<SUB>2</SUB>((4,4′-(OH)<SUB>2</SUB>PO)<SUB>2</SUB>bpy)]<SUP>2+</SUP> undergo efficient injection and back electron transfer with pH independent kinetics characteristic of the local pH in the initial loading solution.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2014/jacsat.2014.136.issue-39/ja506987a/production/images/medium/ja-2014-06987a_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja506987a'>ACS Electronic Supporting Info</A></P>

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