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        Methyl Jasmonate and α-aminooxi-β-phenyl Propionic Acid Alter Phenylalanine Ammonia-Lyase Enzymatic Activity to Affect the Longevity and Floral Scent of Cut Tuberose

        Mehran Kanani,Mohammad Javad Nazarideljou 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.2

        Longevity is a determining factor of the marketability of cut flowers, and is therefore a key target incommercial floriculture. Floral scent is another important factor in the popularity of cut flowers. The phenylpropanoidpathway is one of the key pathways in floral scent production. The first step of this pathway is catalyzed by theenzyme phenylalanine ammonia-lyase (PAL). Here, we evaluated the pre-harvest foliar application of a PAL promoter(methyl jasmonate [MeJA]) and a PAL inhibitor (α-aminooxi--phenyl propionic acid [AOPP]) on the physiologicalparameters, floral scent, and longevity of cut tuberose flowers (Polianthes tuberosa L.). Relative water content (RWC)was significantly affected by foliar application of treatments. The highest and lowest RWCs were observed in plantstreated with AOPP (3 mM) and the control, respectively. Catalase (CAT) enzyme activity was not significantly affectedby treatments, while peroxidase (POD) activity was significantly affected. Foliar application significantly affectedstem water uptake and the 3 mM AOPP resulted in the highest stem water uptake. A GC-MS analysis of floralscent volatiles detected 37 different compounds, with the most abundant being methyl benzoate, benzyl benzoate,pentacosane, and methyl salicylate. The pre-harvest foliar application of MeJA increased the total scent of tuberose, aswell as increasing the relative abundances of methyl benzoate and methyl salicylate and reducing the benzyl benzoateand pentacosane content. In contrast, the foliar application of AOPP decreased the total floral scent; while the relativeamount of pentacosane increased, the methyl benzoate and methyl salicylate contents decreased. Both treatmentsextended the longevity of the tuberose flowers; however, the flowers of the AOPP-treated plants lasted longer thanthose treated with MeJA which could be due the different effects of these treatments on RWC, antioxidant enzymesactivity, and/or stem water uptake. These results indicate that PAL plays an important role in the generation of floralscent and the extension of longevity in cut tuberose.

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