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RISS 인기검색어
- 검색키워드
- 저자 : Mehdi Sahmani (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Microchips and their Significance in Isolation of Circulating Tumor Cells and Monitoring of Cancers
Sahmani, Mehdi,Vatanmakanian, Mousa,Goudarzi, Mehdi,Mobarra, Naser,Azad, Mehdi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3
In micro-fluid systems, fluids are injected into extremely narrow polymer channels in small amounts such as micro-, nano-, or pico-liter scales. These channels themselves are embedded on tiny chips. Various specialized structures in the chips including pumps, valves, and channels allow the chips to accept different types of fluids to be entered the channel and along with flowing through the channels, exert their effects in the framework of different reactions. The chips are generally crystal, silicon, or elastomer in texture. These highly organized structures are equipped with discharging channels through which products as well as wastes of the reactions are secreted out. A particular advantage regarding the use of fluids in micro-scales over macro-scales lies in the fact that these fluids are much better processed in the chips when they applied as micro-scales. When the laboratory is miniaturized as a microchip and solutions are injected on a micro-scale, this combination makes a specialized construction referred to as "lab-on-chip". Taken together, micro-fluids are among the novel technologies which further than declining the costs; enhancing the test repeatability, sensitivity, accuracy, and speed; are emerged as widespread technology in laboratory diagnosis. They can be utilized for monitoring a wide spectrum of biological disorders including different types of cancers. When these microchips are used for cancer monitoring, circulatory tumor cells play a fundamental role.
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In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b
Maryam Golshani,Nafise Vaeznia,Mehdi Sahmani,Saeid Bouzari 대한백신학회 2016 Clinical and Experimental Vaccine Research Vol.5 No.1
Purpose: At present, there is no vaccine available for the prevention of human brucellosis. Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens and it is considered as priority antigen for designing a new subunit vaccine. Materials and Methods: In the current study, we aimed to predict and analyze the secondary and tertiary structures of the Brucella abortus Omp2b protein, and to predict T-cell and B-cell epitopes with the help of bioinformatics tools. Subsequently, cloning and expression of the short form of Omp2b (SOmp2b) was performed using pET28a expression vector and Escherichia coli BL21 host, respectively. The recombinant SOmp2b (rSOmp2b) was purified with Ni-NTA column. Results: The recombinant protein was successfully expressed in E. coli host and purified under denaturation conditions. The yield of the purified rSOmp2b was estimated by Bradford method and found to be 220 μg/mL of the culture. Conclusion: Our results indicate that Omp2b protein has a potential to induce both B-cell. and T-cell.mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis.
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