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Matsuda, Kiku,Chaudhari, Atul A.,Lee, John Hwa Elsevier 2010 Research in veterinary science Vol.89 No.2
<P><B>Abstract</B></P><P>An intestinal pathogenic <I>Escherichia coli</I> isolate from calf diarrhea, containing the <I>iutA</I>, <I>f17A</I>, <I>afa</I>-<I>8D</I>, and <I>cnf2</I> genes, was able to cause avian colibacillosis after experimental infection in chickens. Intra-tracheal inoculation and spray of this strain caused 10% of mortality and gross lesions, including airsacculitis, pericarditis, and perihepatitis. These results suggest that some bovine pathogenic <I>E. coli</I> can cause extra-intestinal infections in other animal species.</P>
Matsuda, Kiku,Chaudhari, Atul A.,Kim, Sam Woong,Lee, Kyeong Min,Lee, John Hwa EDP Sciences 2010 VETERINARY RESEARCH Vol.41 No.5
<P>To construct a novel live vaccine candidate for fowl typhoid (FT) caused by <I>Salmonella</I> Gallinarum (SG), the <I>lon</I> and <I>cpxR</I> genes that are related to host-pathogen interaction were deleted from a wild type SG using the allelic exchange method. The mutants were grown normally, as was the wild type. The biochemical properties of the mutants remained very similar to those of the wild-type, while JOL914 (Δ<I>lon</I>) and JOL916 (Δ<I>lon</I>Δ<I>cpxR</I>) were mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, and 46.2-fold in JOL914, JOL915 (Δ<I>cpxR</I>), and JOL916, respectively. Dot-blot analysis demonstrated significant increases of FimA expression at 6.77-, 2.33-, and 3.90-fold for JOL914, JOL915, and JOL916, respectively. Internalizations of JOL914, JOL915, and JOL916, in chicken abdominal macrophages, were increased at 4.65-, 0.50-, and 2.72-fold, respectively. Virulences of JOL914, JOL915 and JOL916, analyzed by LD<SUB>50</SUB> using 1-week-old chickens, were attenuated approximately at 10<SUP>1</SUP>-, 10<SUP>1</SUP>-, and > 10<SUP>3</SUP>-fold, respectively. The oral inoculations of 2 × 10<SUP>7</SUP> cfu of the wild type, JOL914, JOL915 and JOL916 caused 55.6, 16.7, 22.2, and 0.0% mortality, respectively. Significantly moderate gross lesions of the liver and spleen were observed in the JOL916 group compared to the other groups. An induced immune response and significant peripheral mononuclear proliferation reaction were observed in the JOL916 group. At the protection against the wild type challenge, JOL916 offered 100% protection. Thus, the results of this study suggest that JOL916 among the mutants studied represented the safest and most effective live vaccine candidate against FT.</P>
임신 개에서의 Brucella abortus Strain RB51 접종시험
허진,박용호,Kiku, Matsuda,백병걸 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.3
Brucella abortus strain RB51 (SRB51) is an approved brucellosis vaccine in cattle. This study was caried out to know the immunological responses and abortion status in dogs inoculated with SRB51. Six pregnant dogs were subcutaneously inoculated with 1.0×109 CFU of SRB51. The animals were monitored at regular intervals by the standard tube agglutination test (STAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and dot-blot. Using the whole cell antigen of SRB51, the mean antibody titer against SRB51 was below 1:10 at prior to inoculation in dot-blot. The titer gradually increased up to 1:370 by the 3rd week post-inoculation. In STAT and RBT, using the whole cell antigen of B. abortus 1119-3, and 2ME-RSAT, using the whole cell antigen of B. canis M-, the sera against SRB51 did not react with both antigens. SRB51 was not isolated from the blood of dogs subcutaneously inoculated with the bacteria. In addition, subcutaneous inoculation of SRB51 did not lead to abortion in pregnant dogs.
Chaudhari, Atul A,Matsuda, Kiku,Lee, John Hwa American Association of Avian Pathologists [etc.] 2013 Avian diseases Vol.57 No.1
<P>An attenuated Salmonella (deltalon, deltacpxR, and deltaasdA16) delivery system containing the genes encoding P-fimbriae (papa and papG), aerobactin receptor (iutA), and CS31A surface antigen (clpG) of avian pathogenic Escherichia coli (APEC) was constructed, and its potential as a vaccine candidate against APEC infection in chickens was evaluated. The birds were divided into three groups designated group A (nonvaccinated control), group B (given a single immunization), and group C (administered prime and boost immunizations). Prime and booster vaccinations with the constructions were administered to 1-day-old and 14-day-old birds, respectively. Immune responses were measured postimmunization, and the birds were challenged via an intra-air sac route with a virulent APEC strain at the second, third, and fourth weeks of age. Group B birds were partially protected against the challenge and showed increased levels of plasma immunoglobulin (Ig)G, mucosal IgA antibodies, and lymphocyte proliferation. Group C birds showed greater protection against the challenge, with significantly stronger immune responses compared with the birds in the other groups. Overall, our data suggest that the Salmonella delivery system with recombinant constructs is capable of inducing robust immune responses and induces effective protection against colibacillosis caused by APEC.</P>