http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yan Wang,Mary Yuk Kwan Lee,Judith Choi Wo Mak,Mary Sau Man Ip 대한당뇨병학회 2019 Diabetes and Metabolism Journal Vol.43 No.5
Background: The relationship between obstructive sleep apnoea (OSA) and metabolic disorders is complex and highly associated. The impairment of adipogenic capacity in pre-adipocytes may promote adipocyte hypertrophy and increase the risk of furthermetabolic dysfunction. We hypothesize that intermittent hypoxia (IH), as a pathophysiologic feature of OSA, may regulate adipogenesisby promoting macrophage polarization. Methods: Male C57BL/6N mice were exposed to either IH (240 seconds of 10% O2 followed by 120 seconds of 21% O2, i.e., 10 cycles/hour) or intermittent normoxia (IN) for 6 weeks. Stromal-vascular fractions derived from subcutaneous (SUB-SVF) and visceral(VIS-SVF) adipose tissues were cultured and differentiated. Conditioned media from cultured RAW 264.7 macrophages afterair (Raw) or IH exposure (Raw-IH) were incubated with SUB-SVF during adipogenic differentiation. Results: Adipogenic differentiation of SUB-SVF but not VIS-SVF from IH-exposed mice was significantly downregulated incomparison with that derived from IN-exposed mice. IH-exposed mice compared to IN-exposed mice showed induction of hypertrophicadipocytes and increased preferential infiltration of M1 macrophages in subcutaneous adipose tissue (SAT) comparedto visceral adipose tissue. Complementary in vitro analysis demonstrated that Raw-IH media significantly enhanced inhibition ofadipogenesis of SUB-SVF compared to Raw media, in agreement with corresponding gene expression levels of differentiation-associatedmarkers and adipogenic transcription factors. Conclusion: Low frequency IH exposure impaired adipogenesis of SAT in lean mice, and macrophage polarization may be a potentialmechanism for the impaired adipogenesis.