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- 저자 : Manjunatha P. B. (검색결과 4 건)
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B. M. Manjunatha,J. P. Ravindra,P. S. P. Gupta,M. Devaraj,T. G. Honnappa,A. Krishnaswamy 대한수의학회 2009 JOURNAL OF VETERINARY SCIENCE Vol.10 No.2
The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO2 incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24℃ in the presence of cyto-b. Initially, the embryos were exposed to 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in a base medium for 4 min. After the initial exposure, the embryos were transferred to a 7 μl drop of 25% EG and 25% DMSO in base medium and 0.3 M sucrose for 45 sec. After warming, the embryos were cultured in vitro for 72 h. The post-thaw in vitro developmental competence of the cyto-b-treated embryos did not differ significantly from those vitrified without cyto-b treatment. The hatching rates of morulae vitrified without cyto-b treatment was significantly lower than the nonvitrified control. However, the hatching rate of cyto-btreated vitrified morulae did not differ significantly from the non-vitrified control. This study demonstrates that freezing of buffalo embryos by cytoskeletal stabilization and vitrification is a reliable method for long-term preservation.
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Exploration of Heat Stress-Responsive Markers in Understanding Trait Associations in Wheat
Manjunatha P. B.,Nivedita Sinha,Hari Krishna,Divya Chauhan,Pawan Kumar,Ranjeet R. Kumar,Neelu Jain,P. K. Singh,G. P. Singh 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2
Heat stress (HS) is detrimental to wheat production and productivity globally. To combat HS, several genetic, molecular, and genomic approaches have been employed in the past. Analyzing the physiochemical mechanisms and the important regulatory genes involved is the key to develop HS tolerant plants. In the present work, a total of 243 novel simple sequence repeat (SSR) markers developed from stress-associated genes identified through RNA-seq were used for understanding marker–trait associations. 37 SSRs were found to be clearly polymorphic and among these, 28 SSR loci were significantly associated with component traits of HS tolerance. The polymorphic SSRs were validated for diversity analysis on a subset of 85 genotypes. The genotypes were grouped into four clusters representing diverse and similar alleles imparting HS tolerance in Indian and exotic genotypes. Additionally, 28 genes selected for the expression analysis confirmed that 15 genes were induced under HS in the thermotolerant WH1021 and Raj3765 and repressed in thermosusceptible HD2009 cultivar. Hence, the information on traits associated with candidate genes and the SSR markers overlying on the gene will enhance our understanding of thermotolerance mechanism operating in wheat and will help the breeders in the precise development of heat-tolerant genotypes through marker-assisted selection (MAS).
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B. Chinnappa Reddy,H.C. Manjunatha,Y.S. Vidya,K.N. Sridhar,U. Mahaboob Pasha,L. Seenappa,B. Sadashivamurthy,N. Dhananjaya,K.V. Sathish,P.S. Damodara Gupta 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.3
In the present communication, pure and stable a-Bismuth Oxide (Bi2O3) nanoparticles (NPs) weresynthesized by low temperature solution combustion method using urea as a fuel and calcined at 500oC. The synthesized sample was characterized by using powder X-ray Diffraction (PXRD), Scanning ElectronMicroscopy (SEM), Energy dispersive X-ray analysis (EDAX), Transmission Electron Microscopy (TEM),Fourier Transform Infrared Spectroscopy (FTIR) and UVeVisible absorption spectroscopy. The PXRDpattern confirms the formation of mono-clinic, stable and low temperature phase a-Bi2O3. The directoptical energy band gap was estimated by usingWood and Tauc's relation which was found to be 2.81 eV. The characterized sample was studied for X-ray/gamma ray shielding properties in the energy range0.081e1.332 MeV using NaI (Tl) detector and multi channel analyzer (MCA). The measured shieldingparameters agrees well with the theory, whereas, slight deviation up to 20% is observed below 356 keV. This deviation is mainly due to the influence of atomic size of the target medium. Furthermore an accuratetheory is necessary to explain the interaction of X-ray/gamma ray with the NPs.The present workopens new window to use this facile, economical, efficient, low temperature method to synthesizenanomaterials for X-ray/gamma ray shielding purpose
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Sathish K.V.,Sridhar K.N.,Seenappa L.,Manjunatha H.C.,Vidya Y.S.,Chinnappa Reddy B.,Manjunatha S.,Santhosh A.N.,Munirathnam R.,Raj Alfred Cecil,Damodara Gupta P.S.,Sankarshan B.M. 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.5
For the first time Aluminium-BariumeZinc oxide nanocomposite (ZABONC) was synthesized by solution combustion method where calcination was carried out at low temperatures (6000 C) to study the electromagnetic (EM) (X/g) radiation shielding properties. Further for characterization purpose standard techniques like PXRD, SEM, UV-VISIBLE, FTIR were used to find phase purity, functional groups, surface morphology, and to do structural analysis and energy band gap determination. The PXRD pattern shows (hkl) planes corresponding to spinel cubic phase of ZnAl2O4, cubic BaðNO3Þ2, a and g phase of Al2O3 which clearly confirms the formation of complex nano composite. From SEM histogram mean size of nano particles was calculated and is in the order of 17 nm. Wood and Tauc’s relation direct energy band gap calculation gives energy gap of 2.9 eV. In addition, EM (X/g) shielding properties were measured and compared with the theoretical ones using standard procedures (NaI (Tl) detector and multi channel analyzer MCA). For energy above 356 keV the measured shielding parameters agree well with the theory, while below this value slight deviation is observed, due to the influence of atomic/crystallite size of the ZABONC. Hence synthesized ZABONC can be used as a shielding material in EM (X/g) radiation shielding
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