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Application of polyaniline as enzyme based biosensor
Manju Gerard,말호트라 한국물리학회 2005 Current Applied Physics Vol.5 No.2
The PANI lms have been synthesized electrochemically and are used as matrix for immobilization of glucose oxidase (GOD)and lactate dehydrogenase (LDH) enzymes. The temporal aspects of anion self-exchange in PANI lms have been investigated. Theexchange of bulkier tosylateferricyanide ion with Cl. ion has been monitored by photometry and electrochemical techniques. Therelative changes in porosity brought about by self-exchange have been experimentally determined to be 323 and 2125/k in tosylate-exchanged and ferricyanide-exchanged polyaniline lms, respectively. It is seen that the polyaniline lms exhibit enhanced loading ofglucose oxidase after a self-ion exchange, and, hence they can be used for the fabrication of a third generation glucose biosensor.Lactate is determined by the photometric detection of NADH formed in the reaction catalysed by LDH. Studies have been car-ried out with PANI as a matrix for the immobilization of LDH and its feasibility as a biosensor. The results of the photometric andamperometric measurements conducted on such LDH/PANI electrodes show a response to pyruvate concentration upto 0.45 mM, aresponse time of 90 s and a shelf life of about two weeks..