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Mandaokar, Ajin,Thines, Bryan,Shin, Byongchul,Markus Lange, B.,Choi, Goh,Koo, Yeon J.,Yoo, Yung J.,Choi, Yang D.,Choi, Giltsu,Browse, John Blackwell Publishing Ltd 2006 The Plant journal Vol.46 No.6
<P>Summary</P><P>In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in jasmonate synthesis, such as <I>opr3</I>, are male-sterile but become fertile when jasmonate is applied to developing flower buds. We have used ATH1 oligonucleotide arrays to follow gene expression in <I>opr3</I> stamens for 22 h following jasmonate treatment. In these experiments, a total of 821 genes were specifically induced by jasmonate and 480 genes were repressed. Comparisons with data from previous studies indicate that these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen. Bioinformatics tools allowed us to associate many of the induced genes with metabolic pathways that are probably upregulated during jasmonate-induced maturation. Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes. Extensive additional analysis of our dataset identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate. Two of these transcription factors, MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins. A <I>myb21</I> mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility. A <I>myb24</I> mutant was phenotypically wild-type, but production of a <I>myb21</I><I>myb24</I> double mutant indicated that introduction of the <I>myb24</I> mutation exacerbated all three aspects of the <I>myb21</I> phenotype. Exogenous jasmonate could not restore fertility to <I>myb21</I> or <I>myb21</I><I>myb24</I> mutant plants. Together with the data from transcriptional profiling, these results indicate that MYB21 and MYB24 are induced by jasmonate and mediate important aspects of the jasmonate response during stamen development.</P>