정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

Dunaliella salina의 성장에 미치는 탄소원 및 빛의 영향

이지현,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

The marine microalga Dunaliella salina was studied to optimize its growth conditions in batch cultrue. Various culture conditions were studied by adding glucose and NaHCO??. Another culture methods like photoautotrophic, photoheterotrophic and heterotrophic conditions were studied. The adding 0.5% glucose in the medium made the growth rate increased more than 2 times, but the adding NaHCO?? had no effect on the growth rate of Dunaliella salina. Dunaliella salina did not grow in heterotrophic condition but grew rapidly in photoheterotrophic condition with light and glucose.

키틴 분해균 Bacillus sp. LJ-25의 분리 및 효소생산 최적화

이정석,주동식,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

In order to produce functional chitin oligosaccharides, a chitinolytic becterium was newly screened from the viscera of Korean bony fish, and identified as Bacillus sp. LJ-25. For the production of chitinolytic enzyme, 1.0% nutrient broth and 0.3% colloical chitin was used as nitrogen and carbon source, respectively. The optimal temperature, initial pH and conentration of NaCl for the production of chitinase from Bacillus sp. LJ-25 were 30℃, 6.5-7.0 and 1.0%, rspectively. Chitinolytic activity of Bacillus sp. LJ-25 was increased until the incubation of 168 hours, but after 168 hours, the activity was decreased.

한국산 노루발풀의 유전적 변이와 집단구조

이인섭,문성기,허만규 慶星大學校 1997 論文集 Vol.18 No.2

Pyrola japonica (Pyrolaceae) is a perennial herbaceous species to Asia. This species reproduces both asexually by underground rhizomes and sexually by seeds. The level of genetic diversity of this species was surveyed using starch gel electrophoresis at putative six enzyme loci from 11 natural populations in Korea. Results from six loci indicated that genetic diversity was high and population divergence was found between genetic identity statistics and geographic distance between populations. Although the range of genetic diversity statistics among populations was unsually large, genetic drift and asexual reproduction did not appear to play major roles in structuring genetic variation. We concluded that the level of genetic diversity maintained within P. japonica populations and the level of population divergence was influenced by its status as forest and mode of gene flow.

Chlorella vulgaris의 배양을 위한 Photobioreactor에 관한 연구

조만기,이정석,조덕제 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

Until now, microalgae(Chlorella vulgaris), commonly known as seaweeds, are a rich and diverse source of unique feed for rotifer and microalgae and were cultivated mainly in an conventional open raceway pond photobioreactor. In this work, new photobioreactors were developd to avoid disadvantages of conventional photobioreactor. Higher growth rates could be achieved. As the light is the key factor for culturing microalgae, we focused on a sufficient supplement of kight for the microalgae. The developed photobioreactors were cylinder photobioreactor, spherical surface photobioreactor-Ⅰ, spherical surface photobioreactor-Ⅱ, plate type photobioreactor, raceway pond type photobioreactor and water wheel type photobioreactor. It was shown that the spherical surface photobioreactor-Ⅱ was the best one in respect to ??[1.05day??], also more resistant against drying up and pollution.

빛의 파장에 따른 광합성 미세조류의 성장

김현주,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

The growth of photoautotrophic organism such as microalgae occurs through photosynehesis in which light is the basic limiting factor in terms of photobiology and photobiotechnology. The light harvesting pigments found in microalgae are chlorphyll, phycobiliprotein and carotenoid. The concentration of photosynthetic pigments and growth are effected by the environment-specifically, photonflux density. Therefore, adaptations of microalgae to photon flux density were studied and compared in Chlorella vulgaris and Spriulina platensis. Chlorella vulgaris and Spirulina platensis were cultured under illumination of red, green, blue, and white spectrum light with equivalent photon flux density(77 μ Em??s?? between 400 and 700nm). Cultures of Cholorella vularis grown under red, green, blue and white light expressed similar maximum specific growth rates (1.3842 day??, 1.3250 day??, 1.4226, 1.4609 day??, respectively). However, maximum specific growth rates of spirlina platensis in red(0.8142 day??) and white (0.7493 day??) light-grown culture were higher than those in green (0.4404 day??) and blue light (0.4485 day??). Since phycobiliproteins aborbed and orange light were associated with photosystemⅡ and transfered absorbing light energy predominatly to photosytem Ⅱ, red and white light including light-absorbing spectrum of phycobiliprotein were effective for growth of Spirulina platensis.

Bacillus sp. LJ-25가 생성하는 extracellular chitinase의 정제 및 특성

주동식,조덕제,이정석,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

The extracellular chitinase from Bacillus sp. LJ-25 was pruified Sephadex G-100 gel filtration, DEAE-Cellulose and CM-Spephadex C25 column chromatography. The pruified chitinase showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be about 50KDa. The Optimum pH and temperature for the activity of the purified extracellular chitinase were 7.5 and 35℃, respectively. The activity of chitinase was strongly inhibited by metal ions such as Zn²+, Ba²+, Co²+, Mn²+ and Cu²+. The purified chitinase did not hydrolyze N,N??-diacetylchitobiose, N,N, N'-triacetylchitotriose and p-nitrophenol-N-acetyl-β-D-glucosaminide, which are known to be the substrates of exo-type chitinase. The products in hydolysis of colloidal chitin with the chitinase were N-acetylglucosamine, N,N'-diacetylchitobiose and N,N,N'-triacetylchitotriose.

물리ㆍ화학적 방법을 이용한 위생적인 해상빙의 제조방법 개발

조덕제,조만기,이정석 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

In order to make hygienic fishery ice, sea and city water were pretreated by physical method such as ultrafitration, ultraviolet sterilizer and heating. Among of the three treatments, U,V-light is the best method for application in ice-making enterprise practically. The fishery ice containing 3% salt decreased the contamination of microoganism and improved the ability of low temperature preservation. Key words: hygienic fishery ice, physical and chemical method.

컴퓨터 시뮬레이션을 통한 치어사육용 다단계 배양시스템의 개발 가능성 탐색

정은수,조덕제,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

It was researched that the possibility for developing multistage culture system to cultivate larvae by computer simulation with basic experiments. This culture system was composed of 3 stages. Chlorella sp. at the 1st stage are being supplied to the rotifer(Brachionus plicatilis) at 2nd stage, and the rotifer are bing feeded to larvae at the 3rd stage. In this study, Chlorella sp. were cultivated by batch culture to search for the possibility of continuous feeding rate Chlorella sp., ???????? and ??(cells/hㆍrotifer) in the multistage culture system, then the change of the rotifer concentration at 2nd stage was simulated by computer. The required amount of rotifer for the growth of larvae was also increased as the increase of the length of larvae. On the 9th day of the culture, the rotifer uptake rate of larvae was 250(cells/dayㆍlarvae). Based on these basic experiments and results. It was suggested that the possibility of multistage culture system to cultivate larvae with continuous feeding of Chlorella sp. and rotifer.

축구선수와 일반인의 스쿼트운동에 따른 근피로도 변화 비교

박수경(Soo Gyeong Park),이만기(Man Gi Lee),김현준(Hyun Jun Kim) 한국사회체육학회 2010 한국사회체육학회지 Vol.0 No.41

In this study, to analyze the changing process of the muscle fatigue before squat, immediately after squat, 5 minutes after squat, 10 minutes after squat, experiment was carried out to 8 people of soccer player played over 7 years who are at I university and 8 people of healthy adult man. The electromyogram was measured at Erector Spinae, Gluteus Maximus and Rectus Femoris, and repeated 2-way ANOVA test was performed by repeated measuring about the value of the muscle fatigue of each muscle and time, group, left and right side, time and group, time and left and right side. The results are as below. About the left and right side of the Erector Spinae, Gluteus Maximus and Rectus Femoris, there is no meaningful difference through all 3 kind of muscles. In the result of the analysis of the study about the Erector Spinae and Rectus Femoris, there are statistically definite differences through time and a little statistical difference through time and group. But about the Gluteus Maximus, there is no statistically meaningful difference. Thus, the muscle fatigue generated in the process of squat, influences on the muscle of Erector Spinae and Rectus Femoris but doesn`t influence on the Gluteus Maximus.