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RISS 인기검색어
- 검색키워드
- 저자 : Maasfeld, Wolfgang (검색결과 4 건)
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Validation protocol for whole-body dosimetry in an agricultural exposure study
이지호,김은혜,신용호,이종화,이정학,Wolfgang Maasfeld,김정한 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.1
Agricultural workers exposed to pesticides can experience adverse health impacts depending on toxicity and exposure amount. Whole-body dosimetry (WBD) is the most reliable, practical, and realistic method for measuring exposure. Since validation of analytical and experimental methodologies is critical for quantitative determination of exposure, we conducted a validation procedure to design an essential protocol for WBD exposure studies. Using the fungicide kresoxim-methyl, matrixmatched standards were prepared with various matrices including outer cloth, inner cloth, washing solution for gloves and hands, gauze, and glass fiber filter (IOM sampler) to determine the instrumental limit of quantitation for high-performance liquid chromatography (HPLC) (2 ng) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) (10 pg). Method limits of quantitation (MLOQ) were also set for HPLC (0.1 mg/L) and LC–MS/ MS (0.005 mg/L). We observed good analysis repeatability (coefficient of variation\6%), and the linearity of the calibration curves was reasonable (r2[0.998) in the range of 0.001–10 mg/L in various matrices. Recovery tests were carried out at three levels of concentration (MLOQ, 10 MLOQ, and 100 MLOQ) and resulted in good recoveries (72.7–105.6%). We did not observe breakthrough of the compound in tests of holding capacity for glass fiber pesticide filters. The procedures established in the present study are applicable as an essential, comprehensive protocol for exposure assessment studies using WBD.
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Validation protocol for whole-body dosimetry in an agricultural exposure study
Lee, Jiho,Kim, Eunhye,Shin, Yongho,Lee, Jonghwa,Lee, Junghak,Maasfeld, Wolfgang,Kim, Jeong-Han The Korean Society for Applied Biological Chemistr 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.1
Agricultural workers exposed to pesticides can experience adverse health impacts depending on toxicity and exposure amount. Whole-body dosimetry (WBD) is the most reliable, practical, and realistic method for measuring exposure. Since validation of analytical and experimental methodologies is critical for quantitative determination of exposure, we conducted a validation procedure to design an essential protocol for WBD exposure studies. Using the fungicide kresoxim-methyl, matrixmatched standards were prepared with various matrices including outer cloth, inner cloth, washing solution for gloves and hands, gauze, and glass fiber filter (IOM sampler) to determine the instrumental limit of quantitation for high-performance liquid chromatography (HPLC) (2 ng) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) (10 pg). Method limits of quantitation (MLOQ) were also set for HPLC (0.1 mg/L) and LC-MS/MS (0.005 mg/L). We observed good analysis repeatability (coefficient of variation < 6%), and the linearity of the calibration curves was reasonable ($r^2$ > 0.998) in the range of 0.001-10 mg/L in various matrices. Recovery tests were carried out at three levels of concentration (MLOQ, 10 MLOQ, and 100 MLOQ) and resulted in good recoveries (72.7-105.6%). We did not observe breakthrough of the compound in tests of holding capacity for glass fiber pesticide filters. The procedures established in the present study are applicable as an essential, comprehensive protocol for exposure assessment studies using WBD.
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농작업자의 Kresoxim-methyl과 fenthion에 대한 호흡노출량 측정을 위한 IOM 채집기의 효율성 평가
이지호(Jiho Lee),김은혜(Eunhye Kim),이종화(Jonghwa Lee),신용호(Yongho Shin),Wolfgang Maasfeld,최훈(Hoon Choi),문준관(Joon-Kwan Moon),이혜리(Hyeri Lee),김정한(Jeong-Han Kim) 한국농약과학회 2015 농약과학회지 Vol.19 No.3
An IOM sampler equipped with glass fiber filter has been recently utilized instead of solid adsorbent, which was used to measure the inhalation exposure of agricultural operator to pesticides. The aim of this study is to validate the efficacy of an IOM sampler by measuring the trapping efficiency and breakthrough using kresoxim-methyl water-dispersible granule and fenthion emulsifiable concentrate. On LCMS/MS, minimum detection level was 12.5 pg and method limit of detection was 5.0 ng/mL. Good linearity (R2 > 0.999) for matrix matched standards was obtained. Recoveries of pesticides from glass fiber filter were 102-109% (kresoxim-methyl) and 97-104% (fenthion) while those from XAD-2 resin were 94-98% (kresoxim methyl) and 93-100% (fenthion). Trapping efficiency test was performed with personal air pumps and IOM sampler (glass fiber filter) connected with solid adsorbent (XAD-2 resin) with two types of formulation (solid and liquid) which were diluted by standard rate and sprayed to IOM sampler. Those pesticides were trapped only in glass fiber filter without any breakthrough to solid adsorbent. After spiking of pesticides to glass fiber filter, breakthrough test was carried out with IOM sampler (glass fiber filter) which was connected with solid adsorbent. As a results, 87-101% of kresoxim-methyl and 96-105% of fenthion remained in spiked glass fiber filter, however, no pesticides were detected in second glass fiber filter and solid adsorbent. In conclusion, IOM sampler which equipped with glass fiber filter can be applied widely for pesticide inhalation exposure study since it has good trapping efficiency and adsorption capacity, regardless of the solid or liquid formulation.
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