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Mitsuhashi, T.,Mitsumoto, M.,Yamashita, Y.,Ozawa, S. Asian Australasian Association of Animal Productio 1990 Animal Bioscience Vol.3 No.4
The fat thicknesses of twenty-eight Japanese Black beef steers were measured with an ultrasonic device at eleven points on the cattle prior to slaughter and side dissection. The relation between live fat thickness and both weight and percentage of fat and lean in the carcass was examined. Fat thickness obtained from nine points of the chest, flank and rump regions was found to relate significantly (P<0.01) to both weight and percentage of fat. However, shoulder fat thickness measurements were not significantly related to the weight or percentage of fat or lean in the carcass. Addition of live fat thickness to animal age or live weight as an independent variable markedly improved the precision of multiple regression equations for predicting weight of fat and lean, and percentage of fat. In predicting the percentage of lean, both animal age and body weight were not employed in the multiple regression equation. The residual standard deviation for predicting percentage of fat and lean were 1.93 and 1.87, respectively. The ultrasonic measurement of fat thickness if supposed to be useful to the prediction of carcass composition of beef cattle.
Mitsuhashi, T.,Mitsumoto, M.,Yamashita, Y.,Ozawa, S. Asian Australasian Association of Animal Productio 1988 Animal Bioscience Vol.1 No.2
A high performance liquid chromatographic procedure is described for the direct determination of the picomole amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A, using a stainless steel column packed with C-18 derivatized porous silica ($5{\mu}m$), an isocratic elution with a mixture of 33 mM $KH_2PO_4$/acetonitrile as a mobile phase and a UV detector. The long-chain acyl-Coenzyme A esters were determined in incubated microsomal fractions of a bovine liver to demonstrate the utility of this method for monitoring acyl-CoA synthesis in biological samples. The reaction rate of palmitate was higher than that of stearate. After a 60 minute incubation period, the generated amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A were approximately 70 and 20 n mol/mg micresomal protein, respectively. The advantage of this method are in that no decomposition of the CoA esters is involved, while the constituent molecular species is detected.
Study on Gap Height Measurement of Radial Slit Using Pneumatic System
Kousuke Mitsuhashi,Chongho Youn,Toshiharu Kagawa 유공압건설기계학회 2015 유공압건설기계학회 학술대회논문집 Vol.2015 No.10
Flame arresters are required to assure safe handling of flammable gases in industrial plants. In this paper, we propose a new type of frame arrester in which a radial slit–type device of overlapping parallel discs is used to quench flames. If the gap height of the radial slit is even slightly too large, a flame cannot be quenched. Moreover, there is the possibility that the gap height is not homogeneous. With current measurement methods, only the gap height at the outlet can be measured, and the inside state of the gap cannot be determined. Therefore, in this study, we propose a measurement method using a jet, and a Pitot tube to determine the gap height, including the state inside the gap. Based on a theoretical analysis of the radial flow field in the slit, we fin d that the dynamic pressure in the leaving the gap is proportional to the fourth power of the gap height. Therefore, to accurately determine gap height, we propose a method for measuring the dynamic pressure in the let discharged from the slit. The effectiveness of the method was confirmed by measuring radial slits of known heights, and the method can be used to measure radial slits to an accuracy of several micrometers.
Akira Mitsuhashi,Yuji Habu,Tatsuya Kobayashi,Yoshimasa Kawarai,Hiroshi Ishikawa,Hirokazu Usui,Makio Shozu 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.6
Objective: The present study investigated long-term outcomes of medroxyprogesteroneacetate (MPA) plus metformin therapy in terms of control of atypical endometrial hyperplasia(AEH) and endometrial cancer (EC), and post-treatment conception. Methods: We retrospectively analyzed 63 patients (42 with EC; 21 with AEH) who underwentfertility-sparing management using MPA plus metformin. MPA (400 mg/day) and metformin(750–2,250 mg/day) were administered to achieve complete response (CR). Metformin wasadministered until conception, even after MPA discontinuation. Results: Of the total patients, 48 (76%) had a body mass index (BMI) ≥25 kg/m2 and 43 (68%)showed insulin resistance. Sixty-one patients (97%) achieved CR within 18 months. CR ratesat 6, 8–9, and 12 months were 60%, 84%, and 90%, respectively. During a median followupperiod of 57 months (range, 13–115 months), relapse occurred in 8 of 61 patients (13.1%)who had achieved CR. Relapse-free survival (RFS) in all patients at 5 years was 84.8%. Uponunivariate analysis, patients with BMI ≥25 kg/m2 had significantly better prognoses than didthose with BMI <25 kg/m2 (odds ratio=0.19; 95% confidence interval=0.05–0.66; p=0.009). Overall pregnancy and live birth rates per patient were 61% (19/31) and 45% (14/31), respectively. Conclusions: MPA plus metformin is efficacious in terms of RFS and post treatmentconception. Moreover, metformin may be more efficacious for patients with BMI ≥25 kg/m2.
Persistence of perorally inoculated entomopoxvirus spindles in the midgut of Bombyx mori larvae
Wataru Mitsuhashi,Takashi A. Inoue,Kazuhisa Miyamoto,Takafumi N. Sugimoto 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
Peroral inoculation of entomopoxvirus (EV) spindles, microstructures composed of the protein fusolin, enhances the infectivity of some insect viruses by disrupting the physical barrier against microbe infection, the peritrophic matrix, in the insect midgut. Here, we examined the temporal persistence of spindles of Anomala cuprea EV (ACEV) that infect Coleopteran species in Bombyx mori larva midgut because spindle solubility over time in the midgut is closely associated with the degree of the enhancement of microbe infectivity by fusolin. A number of ACEV spindles fed to B. mori larvae were retained in the digestive systems even 20 h after the completion of feeding spindles, and a number of spindles were found to be excreted still almost intact in feces under a light microscope. In an in vitro experiment, most ACEV spindles remained intact in B. mori midgut juice 1 h after the start of incubation and some of spindles appeared even overnight in contrast to Bombyx mori nucleoplyhedrovirus polyhedra, which were immediately dissolved in midgut juice. These results suggest spindles are not generally dissolved readily in the midgut of many insects. The difficulty in solubility of ACEV spindles is considered to be mainly due to that fusolin contains many cysteine residues that form a 3D network of disulfide bonds between fusolin dimers. To use spindles at a low cost as additives in microbial insecticides, increasing the solubility of spindles by protein engineering is important to utilize full spindles inoculated.