http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Liying Dong(Liying Dong),Shufang Liu(Shufang Liu),Jing Li(Jing Li),Didier Tharreau(Didier Tharreau ),Pei Liu(Pei Liu),Dayun Tao(Dayun Tao),Qinzhong Yang(Qinzhong Yang) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6
Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M. oryzae, the direct extraction of M. oryzae genomic DNA from infected rice tissue would be useful alternative for rapid monitoring of changes of avirulence genes without isolation and cultivation of the pathogen. In this study, a fast, low-cost and reliable method for DNA preparation of M. oryzae from a small piece of infected single rice leaf or neck lesion was established. This single step method only required 10 min for DNA preparation and conventional chemical reagents commonly found in the laboratory. The AvrPik and AvrPi9 genes were successfully amplified with the prepared DNA. The expected DNA fragments from 570 bp to 1,139 bp could be amplified even three months after DNA preparation. This method was also suitable for DNA preparation from M. oryzae strains stored on the filter paper. All together these results indicate that the DNA preparation method established in this study is reliable, and could meet the basic needs for polymerase chain reaction-based analysis of M. oryzae.
Liying Niu,Dajing Li,Chunquan Liu,Fuguo Liu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3
Lipoxygenases (LOX) in milk-stage sweet corn and waxy corn were extracted using a phosphate buffer (pH 7.0) and enzyme activities were determined using linoleic acid as a substrate. Michaelis constant (Km) values, decimal reduction times (D value), temperature sensitivity parameters (Z value), and activation energies (Ea) were calculated. Enzymes from both corn types followed first-order inactivation kinetics within 0-25 min and 50- 70℃. However, enzymes exhibited different pH profiles and affinities toward linoleic acid. Km values (4.34 and 1.40 mM for sweet corn and waxy corn, respectively), heat stability values, and Ea values (116.81 and 246.82 kJ/mol) were different. Waxy corn LOX was more heat stable below 65℃ with a higher D value, but was more temperature sensitive with a lower Z value. The different characteristics suggested the presence of different isoenzymes and necessitated the use of different parameters for blanching.
Liu, Boyang,Yang, Runjun,Li, Junya,Zhang, Lupei,Liu, Jing,Lu, Chunyan,Lian, Chuanjiang,Li, Zezhong,Zhang, Yong-Hong,Zhang, Liying,Zhao, Zhihui Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.5
The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs have beneficial effect on human health. Fat1 gene is the coding sequence from Caenorhabditis elegans which might play an important role on lipometabolism. To reveal the function of fat1 gene in bovine fetal fibroblast cells and gain the best cell nuclear donor for transgenic bovines, the codon of fat1 sequence was optimized based on the codon usage frequency preference of bovine muscle protein, and directionally cloned into the eukaryotic expression vector pEF-GFP. After identifying by restrictive enzyme digests with AatII/XbaI and sequencing, the fusion plasmid pEF-GFP-fat1 was identified successfully. The pEF-GFP-fat1 vector was transfected into bovine fetal fibroblast cells mediated by Lipofectamine2000$^{TM}$. The positive bovine fetal fibroblast cells were selected by G418 and detected by RT-PCR. The results showed that a 1,234 bp transcription was amplified by reverse transcription PCR and the positive transgenic fat1 cell line was successfully established. Then the expression level of fat1 gene in positive cells was detected using quantitative PCR, and the catalysis efficiency was detected by gas chromatography. The results demonstrated that the catalysis efficiency of fat1 was significantly high, which can improve the total PUFAs rich in EPA, DHA and DPA. Construction and expression of pEF-GFP-fat1 vector should be helpful for further understanding the mechanism of regulation of fat1 in vitro. It could also be the first step in the production of fat1 transgenic cattle.
Synthesis of fly ash-based microporous copper silicate for CO2 capture from humid flue gases
Liying Liu,Xudong Chen,Yushun Wang,Tao Du,Gang Li 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.3
Conventional microporous adsorbents suffer from CO2 adsorption capacity reduction due to the presence of water vapor in real flue gases. Therefore, development of low-cost moisture-insensitive adsorbents is of great significance. In the present work, microporous copper silicate was synthesized from waste fly ash for the first time. The synthesis conditions were further optimized to obtain copper silicates with high purities. The as-synthesized product was characterized in terms of structural morphology, chemical composition, and surface properties. The CO2 and H2O adsorption properties of the prepared copper silicate were also investigated by single-component isotherm measurements and dynamic CO2/H2O binary breakthrough experiments. The results show that the as-synthesized fly ash-based copper silicate exhibited excellent CO2 adsorption properties even in the presence of water vapor, thus demonstrating promising potential as a moisture-insensitive adsorbent for directly capturing CO2 from humid flue gases.
Quantifying benefits of alternative home delivery operations on transport in China
Liying Song,Jun Wang,Cong Liu,Qian Bian 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10
Lately, last-mile issue is of great concerns to cope with the dramatic developments of internet shopping market in China. Several issues arise from home delivery activities for fulfilling those internet shopping orders, e.g., increased operating costs for handing failed home deliveries, and deteriorated traffic conditions due to frequent delivery trips. To improve the logistics efficiency of home delivery operations and solve the problems of delivery failures, pick-up points and self-delivery boxes are being implemented in China. This study investigates three home delivery models including traditional model, pick-up point (PP) model and self-delivery box (SDB) model. Under each model, carrier’s delivery distance and customer’s collection distance are calculated. According to the distance, cost of the three delivery models are compared. To simulate carrier’s delivery route, an inventive ant colony algorithm combined with genetic algorithm (GAAA) is developed to optimize delivery route in this research.
Diphenyl derivatives from coastal saline soil fungus Aspergillus iizukae
Desheng Liu,Ling Yan,Liying Ma,Yuling Huang,Xiaohong Pan,Weizhong Liu,Zhihua Lv 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
Two new diphenyl derivatives, named iizukinesA (1) and B (2), along with nine known compounds wereisolated from coastal saline soil derived fungus Aspergillusiizukae. The structures were determined by extensivespectroscopic analysis. Their cytotoxicities were preliminarilyevaluated on HL-60, BEL-7402 and A-549 cell linesby the MTT assay.
CONTACT BINARIES IN THE FIELD OF STELLAR CLUSTERS
LIANG, LIU,SHENGBANG, QIAN,LIYING, ZHU The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2
Several contact binary systems in four stellar clusters or their fields are reported here; NGC7789-V12, EP Cep and ES Cep in NGC188, NGC104-V95 and V710 Mon. Their multiple light curves were analyzed by the 2010 version of the W-D code, and their physical parameters were obtained.