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Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11
Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs
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Mechanical evaluation of polymer microneedles for transdermal drug delivery: In vitro and in vivo
Rui Xuan Liu,Yu Ting He,Ling Liang,Liu Fu Hu,Yue Liu,Rui-xing Yu,Bo Zhi Chen,Yong Cui,Xin Dong Guo 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-
In this study, we reported two types of PMNs based on polylactic acid (PLA) and polyvinyl alcohol (PVA),respectively. Parafilm M film, porcine skin, and rats’ models were operated to evaluate the mechanicalproperties in vitro and in vivo to find optimal parameters for efficient insertion. Insertion depth was measuredusing Digital Force Gauge by changing insertion force and speed, respectively. Results showed thatincreasing the insertion force and speed used for PMNs application led to a significant increase in thedepth of insertion. A force of 18 N under a speed of 330 mm/min was the optimal condition for insertingPMNs array into ParafilmM film and porcine skin. In addition, PLA-MNs exhibited higher robustness andenhanced homogeneity in insertion depth compared with PVA-MNs, but PVA-MNs were able to reachmuch deeper insertion depth. Moreover, Sprague Dawley (SD) rat experiments confirmed the effectivenessof optimal insertion parameters for transdermal drug delivery. This study illustrated not only thedevelopment of novel PMNs but also the mechanical evaluation for the design of PMNs.
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Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.
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Relative Diagnosability of Discrete-Event Systems and Its Opacity-Based Test Algorithm
Rui Zhao,Fuchun Liu,Zhusong Liu 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.4
The notion of relative diagnosability is proposed for logical automata and the concept of diagnosablerate is introduced to characterize the diagnosability property of a discrete-event system, which takes values in theinterval [0, 1]. The relationship between relative diagnosability and diagnosability introduced by Sampath et al. that relative diagnosability is weaker than diagnosability for discrete-event systems is analysed. Furthermore, anecessary and sufficient condition for relative diagnosability is presented. In particular, an opacity-based algorithmis developed to test the relative diagnosability, which is polynomial in the number of states of the system. Inaddition, the proposed algorithm may be used to deal with the problem of diagnosability.
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Association between Polymorphisms of Lipoprotein Lipase Gene and Chicken Fat Deposition
Liu, Rui,Wang, Yachun,Sun, Dongxiao,Yu, Ying,Zhang, Yuan Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.10
The objective of this study was to screen single nucleotide polymorphisms (SNPs) of the chicken lipoprotein lipase gene (LPL), using 545 F1 hybrids developed from $4{\times}4$ diallel crossing of four chicken breeds, and to analyze the associations between polymorphisms of the LPL and chicken fat deposition traits. PCR-SSCP was used to detect SNPs in LPL. Fifteen sets of primers were designed to amplify DNA fragments covering the 5'flanking and coding regions of LPL. It showed that there existed 5 polymorphic loci in the 5'flanking region and coding region, respectively. Association analysis was carried out between 10 polymorphic loci and intermuscular fat width, abdominal fat weight, and thickness of subcutaneous fat using ANCOVA, respectively. The results indicated that, in the 5'flanking region, the loci d and e significantly affected thickness of subcutaneous fat (p<0.05), abdominal fat weight (p<0.01) and subcutaneous fat (p<0.05), while in the coding region, synonymous mutation in exon 8 was significantly associated with intermuscular fat width (p<0.05), however, the non-synonymous mutations in exon 7 and exon 9 did not show statistically significant effects on fat deposition traits in this study.
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Liu, Xuan,Usman, Tahir,Wang, Yachun,Wang, Zezhao,Xu, Xianzhou,Wu, Meng,Zhang, Yi,Zhang, Xu,Li, Qiang,Liu, Lin,Shi, Wanhai,Qin, Chunhua,Geng, Fanjun,Wang, Congyong,Tan, Rui,Huang, Xixia,Liu, Airong,Wu, Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4
Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.
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Rui-fang Liu,Xin Ma,Jun-ci Cao,Shuang-xia Niu,Zhi-gang Wu 한국자기학회 2019 Journal of Magnetics Vol.24 No.2
Amorphous materials have the advantages of high permeability and low core losses. Their application in the motor can reduce the energy consumption and improve the efficiency. To investigate the advantage of amorphous materials on the performance of permanent magnet synchronous motors (PMSMs) at lower speed situation, three 1.5 kW motors used in steering pumps of electric vehicles are analyzed and compared. Two prototypes and the experimental platform are built. The experimental results indicate that the efficiency of the PMSM motor with amorphous materials increases by 1.4 % at 1200 rpm, which shows that amorphous materials can also improve the motor performance at lower speed. To further take advantage of amorphous materials, the particle swarm optimization algorithm is used to optimize the dimensions of amorphous motor and the efficiency is increased by 0.54 %.
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Liu Hongxia,Dou Rui,Wang Jifei,Zhu Yachao,Li Jingyao,Liu Jinlong,Wang Yang,Zhang Jintong,Jing Xiaoyuan 한국곤충학회 2022 Entomological Research Vol.52 No.2
The phytophagous caterpillar Illiberis ulmivora is a serious pest of Ulmus glaucescens Franch (Ulmaceae, Ulmus). In order to test potent sex attractant, field trails of synthetic candidate attractants were conducted in China. In this study, baits with ternary blends, (2S)-butyl (7Z)-dodecenoate, (2S)-butyl (9Z)-tetradecenoate, and (2S)-butyl (5Z)-decenoate (8:4:1 ratio) resulted in a higher number of male moths capture than the rest of the baits that were offered. Traps positioned at a height of 1.2 m attracted more males than those installed at heights of 2.2 m and 3.2 m. The slope aspect, temperature and humility had a significant effect on the trap catches. More catches occurred in traps placed on semi-shady and semi-sunny slopes than those placed on sunny and shady slopes. The number of pheromones in the septa decreased with age. The residual amounts of pheromones in the lures combined with 2,6-di-tert - butyl-p-cresol (BHT) were higher than those in the control. However, there were no differences in the male captures between components and components combined with BHT during the first 8 d. These findings on monitoring and trap positions can be used to predict the occurrence and population dynamics of I. ulmivora, to develop ecological management of I. ulmivora.
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