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- 저자 : Liu Jieyu (검색결과 2 건)
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Jieyu Yue,Xin Zhang,Ning Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.2
Present study was conducted to elucidate the molecular regulation mechanisms and the critical genes involved in regulating wheat early responses to Cadmium (Cd) stress. Both ICP-AES and fluorescence labeling were used to find that the Cd2? influx into wheat roots was significantly suppressed by pre-treatment with or in the presence of the Ca2? channel blocker LaCl3, Verapamil and N-ethylmaleimide. RNA-seq technology was used to identify differentially expressed genes (DEGs) during 12 h of 100 lM Cd stress. Raw reads (n = 80,309,620 were obtained. 108,549 unigenes were identified and classified into 25 COG categories. 8584 DEGs were detected. Many DEGs were involved in defense and detoxification mechanisms including signaling protein kinases, transcription factors, metal transporters and biosynthesis-related enzymes. A Gene Ontology annotation analysis based on the DEGs indicated the presence of many categories including cellular process, cell part and binding, catalytic activity and transporter activity. The Kyoto encyclopedia of genes and genomes pathway analysis identified 107 terms that were enriched for all of the 1018 DEGs. Quantitative real-time PCR of 27 selected DEGs revealed that the expression patterns were consistent with the transcript abundance changes as identified by Solexa analysis.
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Yuyang Guo,Baihua Zhao,Shan Zhou,Lieming Wen,Liu Jieyu,Yaqian Fu,Fang Xu,Minghui Liu 대한초음파의학회 2022 ULTRASONOGRAPHY Vol.41 No.3
Purpose: This study compared the diagnostic performance of the Ovarian-Adnexal Reporting and Data System (O-RADS), the Risk of Malignancy Index 4 (RMI4), the International Ovarian of Tumor Analysis Logistic Regression Model 2 (IOTA LR2), and the IOTA Simple Rules (IOTA SR) in predicting the malignancy of adnexal masses (AMs).Methods: This retrospective study included 575 women with AMs between 2017 and 2020. All clinical messages, ultrasound images, and pathological findings were collected. Two senior doctors (group I) and two junior doctors (group II) used the four systems to classify AMs. The postoperative pathological diagnosis was used as the gold standard to evaluate the diagnostic efficiency. A receiver operating characteristic curve was used to test the diagnostic performance. The interrater agreement between the two groups was tested using kappa values.Results: Of all 592 AMs, 447 (75.5%) were benign, 123 (20.8%) were malignant, and 22 (3.7%) were borderline. The intergroup consistency test yielded kappa values of 0.71, 0.92, 0.68, and 0.77 for the O-RADS, RMI4, IOTA LR2, and IOTA SR, respectively. To predict malignant lesions, the areas under the curve of the O-RADS, RMI4, IOTA LR2, and IOTA SR systems were 0.90, 0.89, 0.90, and 0.86 for group I and 0.89, 0.87, 0.88, and 0.84 for group II, respectively. The O-RADS had the highest sensitivity (91.0% in group I and 84.8% in group II).Conclusion: The four diagnostic systems could compensate for junior doctors’ inexperience in predicting malignant adnexal lesions. The O-RADS performed best and showed the highest sensitivity.
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