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Shenchi Zhao,Xin Liu,Gongshe Hu,Xi Liang,Chengguo Liu,Qian Liu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.9
The rheological behaviors, structural propertiesand freeze-thaw stability of starch isolated from Tetoniabarley (Normal genotype, Reg. No. CV-334, PI 646199)and Transit barley (Waxy genotype, Reg. No. CV-348, PI660128) were investigated, along with other commonstarch sources for comparison. Transit barley starchshowed the highest loss tangents (tan d) during a frequencysweep test, which suggested a predominance of elasticproperties over viscous properties. However, the tan d ofTetonia barley starch was similar to that of potato starch,which indicated more solidity in comparison to Transitbarley starch. Transit barley starch had the highest gelatinizationtemperature and the lowest gelatinizationenthalpy (P\0.05). Moreover, Tetonia and Transit barleystarches displayed weak diffraction peak intensities byX-ray diffraction analysis. Additionally, Transit barleystarch showed the lowest % syneresis even when freeze–thawed up to five cycles (P\0.05). However, Tetoniabarley starch had the worst freeze–thaw stability(P\0.05), which was verified via scanning electronmicroscopy analysis of freeze–thawed starch gels. Theresults of present study indicate that barley starch can bepractically applied as a functional ingredient in somespecialty starchy foods.
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Rahman Md Atikur,Alam Iftekhar,Sharmin Shamima Akhtar,Kabir Ahmad Humayan,Kim Yong-Goo,Liu Gongshe,Lee Byung-Hyun 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.6
Drought stress is one of the major constraints for soybean growth and productivity worldwide. The study was aimed to investigate drought-induced physiological and proteomic changes in soybeans, as well as drought relief using exogenous hydrogen peroxide (H2O2). In drought-stressed plants, H2O2 spray on the leaf surface improved relative water content (RWC), net photosynthetic rate (Pn), and stomatal conductance (Gs). Furthermore, exogenous H2O2 reduced drought stress-induced endogenous MDA and H2O2 levels, as well as increased the key antioxidant enzymes (SOD,CAT, APX and POD) activ- ity and proline content in H2O2-treated soybean plants. These findings showed that H2O2 treatment significantly reduced drought stress by increasing the antioxidative defense system and osmotic adjustment. Furthermore, using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a total of 27 differently expressed proteins was identified, wherein 23 were up-regulated and 4 were down-regulated under drought condition. These proteins were found to be involved in photosynthesis, energy and metabolism, plant defense and antioxidant, signaling and transport, and transcription regulation in response to H2O2 treatment in soybean under drought stress, according to in silico interactome analysis. These findings add to our understanding of H2O2-mediated drought stress alleviation, as well as the physiological and molecular responses of soybean to drought stress.
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