MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liping Xie,Zhen Liang,Shiqi Li,Xin Xu,Xianglai Xu,Xiao Wang,Jian Wu,Yi Zhu,Zhenghui Hu,Yiwei Lin,Yeqing Mao,Hong Chen,Jindan Luo,Ben Liu,Xiangyi Zheng 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3 -UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3 -UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Composite action of concrete-filled double circular steel tubular stub columns

Liping Wang,Xing-xing Cao,Fa-Xing Ding,Liang Luo,Yi Sun,Xue-mei Liu,Hui-lin Su 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.1

This paper presents a combined numerical, experimental, and theoretical study on the behavior of the concrete-filled double circular steel tubular (CFDT) stub columns under axial compressive loading. Four groups of stub column specimens were tested in this study to find out the effects of the concrete strength, steel ratio and diameter ratio on the mechanical behavior of CFDT stub columns. Nonlinear finite element (FE) models were also established to study the stresses of different components in the CFDT stub columns. The change of axial and transverse stresses in the internal and external steel tubes, as well as the change of axial stress in the concrete sandwich and concrete core, respectively, was thoroughly investigated for different CFDT stub columns with the same steel ratio. The influence of inner-to-outer diameter ratio and steel ratio on the ultimate bearing capacity of CFDT stub columns was identified, and a reasonable section configuration with proper inner-to-outer diameter ratio and steel ratio was proposed. Furthermore, a practical formula for predicting the ultimate bearing capacity was proposed based on the ultimate equilibrium principle. The predicted results showed satisfactory agreement with both experimental and numerical results, indicating that the proposed formula is applicable for design purposes.

Effect of cohesive particle addition on bubbling characteristics of gas-solid fluidized bed: Meso-scale mechanism

Wei Liping,Li Haoqi,Wu Changsong,Lu Youjun,Luo Kun 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.6

Due to the small particle size and large specific surface area, the cohesive particles affect the flow characteristics in a gas-solid bubbling fluidized bed. They can easily produce agglomerated and smooth heterogeneous flow under the different cohesive particle addition amounts. The influence of the cohesive particle amount on the bubbling characteristics was experimentally investigated for two typical cases: bed surface agglomerate and well-dispersed with the bed. The images of bubble rise were recorded by digital camera and processed by binarization to track bubble motion and obtained the bubble motion characteristics. An energy minimization multiscale (EMMS) model based on binary particle bubble was expanded to the flow of cohesive particles to reveal the basic mechanism of influence of the cohesive particle. By comparing the results predicted by the model with the experimental data, it is found that the change rules of bubble size and bubble rising speed are similar, which proves the feasibility of the newly developed EMMS model. A study of the structure shows that the addition of cohesive particles increases the size of the bubbles and inhibits the collapse of the bubble due to the reduction of the gas-solid resistance coefficient.

In-situ synthesis of NiS-modified MgO/S-doped biochar for boosting the adsorption-photocatalytic activity

Xiaogang Zheng,Liping Wang,Yuanliang Zhou,Meiyin Luo,Hongyan Li,Zongyang Bo,Weixin Zheng,Chenggong Chang,Jing Wen,Jinmei Dong 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

Biochar-based composites have been intensively applied in wastewater treatment. To boost theadsorption-photocatalytic capacity of biochar for dyes elimination, S-doped biochar was commodifiedby NiS and MgO (NiS-Mg/S-BC) via the one-pot sulfurization at high temperature. The functional groupsin NiS-Mg/S-BC provided the abundant vacant sites for RhB adsorption and photocatalysis, and the junctioninterface among NiS, MgO, and S-doped biochar was likely to form the channels for the separationand transfer of photo-induced e-/h+ pairs. NiS-Mg/S-BC presented the better adsorption-photocatalyticcapacity of RhB than S-doped biochar, NiS and MgO modified biochar. Langmuir and second-order modelswell described the adsorption behaviors of NiS-Mg/S-BC. The removal efficiency of optimal 2b-NiS-Mg/SBCfor 400 mg/L RhB solution was 96.98% within 120 min, and its adsorption capacity based on secondordermodel was 802.49 mg g1. The photo-corrosion induced to the declined removal efficiency of 2b-NiS-Mg/S-BC to 94.13% after five cycles.

Inverse decoupling sliding mode control for multilevel buck converters in low‑power applications

Jiarong Wu,Liping Luo,Chunming Wen,Qingyu Wang 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.8

Multilevel buck converters are gradually gaining attention in low-power applications. To realize the decoupling of the flying capacitor voltage and the output voltage, this paper proposes an inverse decoupling sliding mode control approach. A nonlinear mathematical model of the multilevel buck converters is built. The reversibility of the model is analyzed based on the inverse system theory, and linearization and decoupling are achieved. In addition, multiple pseudo-linear subsystems are obtained. Then sliding mode controllers are designed to control the linear subsystems. Furthermore, the global asymptotic stability of the control system is verified using the Lyapunov theory, and the robustness of the closed-loop system is demonstrated. Simulation and experimental results show that the proposed approach provides a better dynamic response when compared with existing methods.

Iterative Reliability-Based Modified Majority-Logic Decoding for Structured Binary LDPC Codes

Haiqiang Chen,Lingshan Luo,Youming Sun,Xiangcheng Li,Haibin Wan,Liping Luo,Tuanfa Qin 한국통신학회 2015 Journal of communications and networks Vol.17 No.4

In this paper, we present an iterative reliability-based modified majority-logic decoding algorithm for two classes of structured low-density parity-check codes. Different from the conventional modified one-step majority-logic decoding algorithms, we design a turbo-like iterative strategy to recover the performance degradation caused by the simply flipping operation. The main computational loads of the presented algorithminclude only binary logic and integer operations, resulting in low decoding complexity. Furthermore, by introducing the iterative set, a very small proportion (less than 6%) of variable nodes are involved in the reliability updating process, which can further reduce the computational complexity. Simulation results show that, combined with the factor correction technique and a well-designed non-uniform quantization scheme, the presented algorithm can achieve a significant performance improvement and a fast decoding speed, even with very small quantization levels (3–4 bits resolution). The presented algorithm provides a candidate for trade-offs between performance and complexity.

Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Guochao Zheng,Wei Hu,Yuanjia Liu,Qin Luo,Liping Tan,Guoqing Li 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

Confinement coefficient of concrete-filled square stainless steel tubular stub columns

Fa-Xing Ding,Yi-xiang Yin,Liping Wang,Yujie Yu,Liang Luo,Zhi-wu Yu 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.30 No.4

The objective of this paper is to investigate the confinement coefficient of concrete-filled square stainless steel tubular (CFSSST) stub columns under axial loading. A fine finite 3D solid element model was established, which utilized a constitutive model of stainless steel considering the strain-hardening characteristics and a triaxial plastic-damage constitutive model of concrete with features of the parameter certainty under axial compression. The finite element analysis results revealed that the increased ultimate bearing capacity of CFSSST stub columns compared with their carbon steel counterparts was mainly due to that the composite action of CFSSST stub columns is stronger than that of carbon steel counterparts. A further parametric study was carried out based on the verified model, and it was found that the stress contribution of the stainless steel tube is higher than the carbon steel tube. The stress nephogram was simplified reasonably in accordance with the limit state of core concrete and a theoretical formula was proposed to estimate the ultimate bearing capacity of square CFSSST stub columns using superposition method. The predicted results showed satisfactory agreement with both the experimental and FE results. Finally, the comparisons of the experimental and predicted results using the proposed formula and the existing codes were illustrated.

Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

Guochao Zheng,Muhamd Alsarakibi,Yuanjia Liu,Wei Hu,Qin Luo,Liping Tan,Guoqing Li 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.3

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liang, Zhen,Li, Shiqi,Xu, Xin,Xu, Xianglai,Wang, Xiao,Wu, Jian,Zhu, Yi,Hu, Zhenghui,Lin, Yiwei,Mao, Yeqing,Chen, Hong,Luo, Jindan,Liu, Ben,Zheng, Xiangyi,Xie, Liping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.