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RISS 인기검색어
- 검색키워드
- 저자 : Linlin Luo (검색결과 3 건)
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Chunmei Wei,Linlin Luo,Huajun Wu,Tong Lin 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.4
Monochamus alternatus Hope is a serious pest management concern in stands of pines. Diflubenzuron (DFB) is an insect growth inhibitor (IGI), which acts by disrupting chitin formation and deposition, affecting the cuticle and the molting process. As DFB causes a reduction in chitin content, the insect ultimately dies because of abortive molting. In order to understand how M. alternatus reacts toxicologically to DFB, transcriptional profiling of M. alternatus larvae exposed to a sublethal dose of DFB was monitored using a specific 60-mer oligonucleotide microarray derived from a cDNA library. Treatment of M. alternatus with DFB resulted in a total of 364 unique genes varied at least twofold in terms of accumulation. Of these 364 genes, 53 were upregulated, and 311were downregulated. A gene ontology (GO) enrichment analysis revealed that the differentially expressed genes were widely distributed among the molecular function, biological processes, and cellular component categories,reflecting a broad spectrum of the analyzed transcriptome. Genes that play important roles in cuticulogenesis and metabolic detoxification were detected by GOs and pathway enrichment analyses. This study, an attempt to relate new possible biomarkers for assessing secondary effects of DFB on M. alternatus,will assist in DFB future use for controlling M. alternatus and other Lepidoptera insects.
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The gene expression profile of Monochamus alternatus in response to deltamethrin exposure
Tong Lin,Huajun Wu,Linlin Luo 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4
To identify additional genes that are potentially associatedwith deltamethrin toxicology, differentially expressedgene (DGE) libraries of Monochamus alternatus after short-term exposure to a sub-lethal concentration of deltamethrinwere prepared. The transcripts were sequenced using the Illumina sequencing platform. After cleaningand quality checks, the total numbers of clean reads were 7.066 and 7.139 million in the control anddeltamethrin-exposed libraries, respectively. We found that 4886 and 2083 unique genes were significantlyup- and down-regulated, respectively. The gene ontology enrichment analysis identified that 4032, 3379 and8323 DEGs were involved in cellular components, molecular functions and biological processes, respectively,and showed that the genes were distributed among more than 50 categories. The Kyoto Encyclopedia of Genesand Genomes pathway enrichment analysis indicated that the top 20 enriched pathways included the glutathionemetabolismpathway and the antigen processing and presentation pathway. Therewere 42 DEGs enriched inthe antigen processing and presentation pathway, including those encoding the 70-kDa heat shock protein, proteindisulfide isomerase and calreticulin, all ofwhichwere up-regulated. There were 61 DEGs enriched in the glutathionemetabolism pathway, and these included the up-regulated DEGs encoding glutamate–cysteine ligase,glutathione synthase and glutathione reductase and the down-regulated DEGs encoding glutathione Stransferase,glutathione peroxidase and ornithine decarboxylase. This study is the first step toward achievingan understanding of the profile of the secondary targets of deltamethrin in M. alternatus, and the results may provideinsights to further explore the functions of the target genes in detoxification and resistance to deltamethrin.
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Lina Liu,Xiaolong Ma,Muhammad Bilal,Linlin Wei,Shijie Tang,Hong-zhen Luo,Yuping Zhao,Xuguo Duan 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
Efficient use of lignocellulosic biomass is a prerequisite for sustainable biofuel production while simultaneously contributing to environmental protection. However, phenolic compounds produced during the chemical treatment of lignocellulose inhibit the growth and metabolism of microorganisms, such as Escherichia coli, which is one of the ideal strains for producing target products by microbial fermentation. To provide new ideas for studying microbial tolerance to environmental stress and providing technical support for constructing the engineering strains with high yields of phenolic compounds, this review elucidates the inhibition mechanism of phenols to E. coli. Secondly, a comprehensive and systematic review of current approaches for improving the phenolic-tolerance of E. coli is provided, including strain domestication, random mutagenesis, regulating the expression of outer membrane proteins, changing the composition of membrane fatty acids, accelerating the efflux of phenolic compounds, engineered bacterial biofloc formation, and transcriptome analysis. Finally, this review ends with some questions that still exist today, and prospective strategies are outlined for further improving the phenols-tolerance of E. coli. This review provided a theoretical basis for research into microbial tolerance to environmental stress and the development of engineered strains with high yield of phenolic compounds.
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