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Delphinidin inhibits BDNF-induced migration and invasion in SKOV3 ovarian cancer cells
Lim, Won-Chul,Kim, Hyunhee,Kim, Young-Joo,Park, Seung-Ho,Song, Ji-Hye,Lee, Ki Heon,Lee, In Ho,Lee, Yoo-Kyung,So, Kyeong A.,Choi, Kyung-Chul,Ko, Hyeonseok Elsevier 2017 Bioorganic & medicinal chemistry letters Vol.27 No.23
<P><B>Abstract</B></P> <P>Brain-derived neurotrophic factor (BDNF), the TrkB ligand, is associated with aggressive malignant behavior, including migration and invasion, in tumor cells and a poor prognosis in patients with various types of cancer. Delphinidin is a diphenylpropane-based polyphenolic ring structure-harboring compound, which exhibits a wide range of pharmacological activities, anti-tumor, anti-oxidant, anti-inflammatory, anti-angiogenic and anti-mutagenic activity. However, the possible role of delphinidin in the cancer migration and invasion is unclear. We investigated the suppressive effect of delphinidin on the cancer migration and invasion. Thus, we found that BDNF enhanced cancer migration and invasion in SKOV3 ovarian cancer cell. To exam the inhibitory role of delphinidin in SKOV3 ovarian cancer migration and invasion, we investigated the use of delphinidin as inhibitors of BDNF-induced motility and invasiveness in SKOV3 ovarian cancer cells <I>in vitro</I>. Here, we found that delphinidin prominently inhibited the BDNF-induced increase in cell migration and invasion of SKOV3 ovarian cancer cells. Furthermore, delphinidin remarkably inhibited BDNF-stimulated expression of MMP-2 and MMP-9. Also, delphinidin antagonized the phosphorylation of Akt and nuclear translocation of NF-κB permitted by the BDNF in SKOV3 ovarian cancer cells. Taken together, our findings provide new evidence that delphinidin suppressed the BDNF-induced ovarian cancer migration and invasion through decreasing of Akt activation.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lim, Ilhan,Park, Joon Yeun,Kang, Hye Jin,Hwang, Jae Pil,Lee, Seung Sook,Kim, Kyeong Min,Choi, Tae Hyun,Yang, Sung Hyun,Kim, Byung Il,Choi, Chang Woon,Lim, Sang Moo S. Karger AG 2013 Acta haematologica Vol.130 No.2
<P>Abstract</P><P><B><I>Aims:</I></B> It was the aim of this paper to identify prognostic factors in patients with relapsed or refractory B-cell non-Hodgkin's lymphomas, treated by radioimmunotherapy (RIT) with radioiodinated human/murine chimeric anti-CD20 monoclonal antibody rituximab (<SUP>131</SUP>I-rituximab). <B><I>Methods:</I></B> Twenty-four patients were enrolled prospectively and were treated with unlabeled rituximab 70 mg and a therapeutic activity (median 7.3 GBq) of <SUP>131</SUP>I-rituximab. Contrast-enhanced <SUP>18</SUP>F-FDG PET/CT scans were performed before and after 1 month of RIT. Tumor sizes and maximum standardized uptake values (SUV<SUB>max</SUB>) of scans were measured. <B><I>Results:</I></B> Four of the 24 patients survived. High SUV<SUB>max</SUB> in a pretreatment scan was found to be related to poorer overall survival (OS) and progression-free survival (p = 0.04 and 0.02, respectively). Furthermore, a large tumor size in a pretreatment scan was associated with poorer OS but not with progression-free survival (p < 0.01 and p = 0.07, respectively). By multivariate analyses, a high SUV<SUB>max</SUB>, a large tumor size in a pretreatment scan and diffuse large B-cell lymphoma histology were significantly associated with poorer OS [p = 0.04/hazard ratio (HR) = 3.54, p < 0.01/HR = 5.52, and p = 0.02/HR = 3.38, respectively). <B><I>Conclusion: </I></B>SUV<SUB>max</SUB> and tumor size determined by a pretreatment <SUP>18</SUP>F-FDG PET/CT result as significant predictors of OS in patients with relapsed or refractory B-cell non-Hodgkin's lymphoma treated by RIT.</P><P>Copyright © 2013 S. Karger AG, Basel</P>
( Kyeong Ah Kang ),( Hye Youn Jung ),( Jong Seok Lim ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.4
Silver nanoparticles (AgNPs) are widely used nanoparticles and they are mainly used in antibacterial and personal care products. In this study, we evaluated the effect of AgNPs on cell death induction in the murine dendritic cell line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial membrane potential. The activation of the intracellular signal transduction pathway was also observed in cells cultured with AgNPs. Taken together, our data demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS generation. This study provides important information about the safety of AgNPs that may help in guiding the development of nanotechnology applications.
Lim, Sun Min,Choi, Jae Woo,Hong, Min Hee,Jung, Dongmin,Lee, Chang Young,Park, Seong Yong,Shim, Hyo Sup,Sheen, Seungsoo,Kwak, Kyeong Im,Kang, Dae Ryong,Cho, Byoung Chul,Kim, Hye Ryun Elsevier 2019 Lung cancer Vol.131 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>Radon, a natural radiation, is the leading environmental cause of lung cancer in never-smokers. However, the radon exposure impact on the mutational landscape and tumor mutation burden (TMB) of lung cancer in never-smokers has not been explored. The aim of this study was to investigate the mutational landscape of lung adenocarcinoma in never-smokers who were exposed to various degrees of residential radon.</P> <P><B>Materials and methods</B></P> <P>To investigate the effect of indoor radon exposure, we estimated the cumulative exposure to indoor radon in each house of patients with lung cancer with a never-smoking history. Patients with at least 2 year-duration of residence before the diagnosis of lung adenocarcinoma were included. Patients were subgrouped based on the median radon exposure level (48 Bq/m<SUP>3</SUP>): radon-high <I>vs.</I> radon-low and targeted sequencing of tumor and matched blood were performed in all patients.</P> <P><B>Results</B></P> <P>Among 41 patients with lung adenocarcinoma, the TMB was significantly higher in the radon-high group than it was in the radon-low group (mean 4.94 <I>vs</I>. 2.6 mutations/Mb, <I>P</I> = 0.01). The recurrence rates between radon-high and radon-low group did not differ significantly. Mutational signatures of radon-high tumors showed features associated with inactivity of the base excision repair and DNA replication machineries. The analysis of tumor evolutionary trajectories also suggested a series of mutagenesis induced by radon exposure. In addition, radon-high tumors revealed a significant protein-protein interaction of genes involved in DNA damage and repair (<I>P</I> < 0.001).</P> <P><B>Conclusions</B></P> <P>Indoor radon exposure increased the TMB in never-smoker patients with lung adenocarcinoma and their mutational signature was associated with defective DNA mismatch repair.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Radon is the leading environmental cause of lung cancer in never-smokers. </LI> <LI> We investigated the mutational landscape in never-smokers who were exposed to residential radon. </LI> <LI> Indoor radon exposure increased tumor mutation burden. </LI> </UL> </P>
( Hye-been Kim ),( Yu-ri Seo ),( Kyeong-je Chang ),( Sang-bae Park ),( Hoon Seonwoo ),( Jin-woo Kim ),( Jangho Kim ),( Ki-taek Lim ) 한국산업식품공학회 2017 산업 식품공학 Vol.21 No.3
Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended “green material.” Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.
Fentanyl 투여에 의한 기관내 삽관시 심혈관 자극 억제 효과
이혜원,이미경,신정순,장성호,임혜자,채병국 대한마취과학회 1990 Korean Journal of Anesthesiology Vol.23 No.4
The effect of fentanyl on arterial pressure and heart rate during laryngoscopy and intubation was studied in 40 adult normotensive patients. Two groups of 20 patients were observed. Patients received thiopental sodium 5 mg/kg (contal group) or fentanyl 2 μg/kg with thiopental 5 mg/kg (fentanyl group) for induction of anesthesia. The changes of systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and heart rate (HR) were measured during the preinduction period, before thiopental (or thiopental+fentanyl), after succinylcholine, immediately after intubation and 5 minutes after intubation. The values were compared with preinduction control values. The results were as follows: The control group showed a significant inerease of SBP, DBP and MAP (p$lt;0.001) but no significant increase was noted in the fentanyl group with tracheal intubation. DBP and MAP returned to normal within 5 minutes and SBP decreased significantly (p$lt;0.05). Significant heart rate elevation following intubtion was observed in both groups. It is suggested from the above results that pretreatment with fentanyl 2 g/kg reduce the blood pressure elevation following intubation but dose not prevent the elevation of heart rate.