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        Preparation and adsorption characters of Cu(II)-imprinted chitosan/attapulgite polymer

        Yingying Shi,Qianghua Zhang,Liangdong Feng,Qingping Xiong,Jing Chen 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.5

        Using attapulgite (ATP) as matrix, chitosan (CS) as functional monomer, and introducing the surface ionimprintingconcept, a new Cu(II)-IIP was prepared, and characterized by SEM, XRD and FT-IR. The adsorption ofCu(II) aqueous solution with Cu(II)-IIP was investigated by flame atomic adsorption spectroscopy (FAAS). The polymerhas good selectivity for Cu(II) from competitive metal ions, and the selectivity coefficient of Cu(II) relation to Pb(II),Cd(II) was 78.45 and 82.44, respectively. Sorption equilibrium isotherms could be described by Langmuir and Freundlichmodels; the Freundlich isotherm has shown the best agreement with experimental data, and experimental value of maximumadsorption capacity for Cu(II) was 35.20 mg/g. The obtained thermodynamic parameter (ΔGo, ΔHo, ΔSo) showedthat the Cu(II) adsorption process is a spontaneous and endothermic process. The kinetic data showed that pseudosecond-order kinetic model agrees very well with the dynamic behavior for the sorption of Cu(II) onto Cu(II)-IIP.

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        Characterization of proteinase A excretion from Saccharomyces cerevisiae in high sugar stress conditions

        Liang Dong,Feng Li,Yongzhe Piao,Dong Sun,Rui Zhao,Cheng Li,Lina Cong,Changxin Zhao 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2

        High sugar concentration culturing was commonly used in modern fermentation industry. However, it leads to the reduction of the foam stability in beer brewing due to the excess secretion of proteinase A. To better understand the characterization of proteinase A excretion from Saccharomyces cerevisiae in high sugar stress conditions, the cultures were grown by using YNB medium with a high concentration of glucose. Pro-PrA isolated from the medium was purified by gel exclusion chromatography, and the PrA activity was detected using fluorescent substrate analysis. The relative molecular weight of the purified PrA and pro-PrA was estimated at 42 and 54 kDa, respectively, by SDS-PAGE. It indicated that the metabolic behavior of PrA in the high glucose culturing was quite different from the normal conditions, and glucose concentration may have a big influence on its secreted process. Further study showed that PrA was released at the logarithmic growth phase of the culturing, and the amount of PrA was 11 times higher compared with the normal culturing. PrA was considered to be activated by itself under acidic conditions. And it was also confirmed in this work that the step-wise pathway for the autoactivation known as a pseudo-PrA has a major contribution to the autoactivation process of PrA zymogen outside the cell.

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