Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells

( Rui Dong Li ),( Zhong Liang Deng ),( Ning Hu ),( Xi Liang ),( Bo Liu ),( Jin Yong Luo ),( Liang Chen ),( Liang Jun Yin ),( Xiao Ji Luo ),( Wei Shui ),( Tong Chuan He ),( Wei Huang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.9

We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with TGFβ1 during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rhTGFβ1 synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of TGFβ1 inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that TGFβ1 inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in G0/G1 phase. Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells. [BMB Reports 2012; 45(9): 509-514]

Comparative Research of Competitiveness and Influence Factors of Computer and Information Service Trade of South Korea, China and Japan

Liang Rui,Zi-Yang Liu 한국컴퓨터정보학회 2014 韓國컴퓨터情報學會論文誌 Vol.19 No.12

The paper chooses four trade competitiveness assessment indices to compare the international competitiveness of Computer and Information Service(C&IS) trade between South Korea, China and Japan. The results show that the competitiveness of China is the strongest, Japan secondary and Korea the weakest among the three countries. However, the competitiveness of Korea can be promoted the most quickly and it has exceeded Japan according to TC and NXRCA indices. Meanwhile, there was still a huge gap between the three countries and the two world strong countries?India and Ireland. The main findings of empirical research on influencing factors show that domestic market demand and trade opening degree are the two most important factors influencing the competitiveness of three-country C&IS trade. At the same time, improving the utilization of C&IS and realizing good interaction of C&IS trade and electronic information, communication and other relevant and auxiliary industries can also contribute to the competitiveness improvement.

Design of a Bidirectional DC/AC Converter with Battery Charging/Discharging/Standing Balance Control

Liang-Rui Chen,Bo-Rui Xu,Chuan-Sheng Liu,Shao-wei Peng,Chia-Hsuan Wu 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Battery cells connected in series have been widely used in high-voltage and high-power applications. In this paper, a single-phase battery energy storage system with battery balance charging, battery balance discharging, and power factor correction capabilities was developed. A prototype suitable for a single-phase 110V power supply was designed and implemented for verification. To verify its performance, three 48V/7Ah battery modules were used as normal batteries, and a 48V/5Ah battery module was used as a retired battery. The experimental results showed that the battery energy storage system has excellent battery balancing capability. Compared with that of the conventional system without balanced control, the balancing performance of the proposed system is increased by about 15.25% and 26.92%, respectively, when the system was operated in the converter and rectifier modes. In addition, the proposed system also had battery fault tolerance, and was compatible with recycled batteries and an independent power supply.

A Fast Detection and Recognition Algorithm for Pedestrian at Night Based on Entropy Weight Fast Support Vector Machine

Liang Rui,Wei Honglei,Zhu Qingxin,Liao Shujiao,Deng Hongyao 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5

In allusion to such problems as real-time requirement dissatisfaction and significant recognition difference caused by dimension difference existing in the imaging and recognition algorithm for pedestrian in dark scene, a fast head detection and recognition method for pedestrian at night based on fast support vector machine (FC-SVM) algorithm optimization and entropy weight is established in this paper according to relevant principle of statistics. Based on entropy weight, this method aims at improving the extraction process based on histogram gradient features in order to establish threebranch SVM for the deep recognition of pedestrian at night; meanwhile, FC-SVM algorithm is combined to optimize the recognition calculation overhead in order to ensure the real-time property of the recognition algorithm. Furthermore, the falsely detected pedestrians are evaluated on the basis of the head detection mode so as to improve pedestrian imaging matching accuracy. The simulation result shows that this method can not only effectively recognize FIR target of pedestrian at night, but also effectively adapt to such different application environments as urban and suburban areas on the basis of ensuring the real-time requirement for pedestrian recognition, thus presenting good practicability.

Scenic Image Research Based on Big Data Analysis - Take China's Four Ancient Cities as an Example

( Rui Liang ),( Hanwen Guo ),( Jiayu Liu ),( Ziyang Liu ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.7

This paper aims to compare the scenic images of four ancient Chinese cities including Lijiang, Pingyao, Huizhou and Langzhong, so as to provide specific development strategies for the ancient cities. In this paper, the ancient cities’ scenic images are divided into three sub-indexes and eight evaluation dimensions. Based on this, the study first uses Python software to collect tourists’ online comments on the four ancient cities. Then, the social network analysis method is used to build a high-frequency keywords matrix of tourist comments and the R language is used to generate a visual network graph. After this, the entropy weight method is used to determine the weights and values of eight evaluation dimensions. Finally, the tourists’ overall satisfaction indexes of the four ancient cities are calculated accordingly. The results show that (1) the overall satisfaction of Lijiang is the highest, while that of Huizhou is the lowest; (2) from the weight of each evaluation dimension, it can be seen that tourists care more about the national culture and historical culture; (3) from tourists’ satisfaction index on each evaluation dimension of the four ancient cities, we can find that the four ancient cities has their own advantages and disadvantages in tourism development. (4) local tourism-related institutions should strengthen their advantages and improve their deficiencies so as to enhance tourists’ overall image of the ancient city.

Mechanical evaluation of polymer microneedles for transdermal drug delivery: In vitro and in vivo

Rui Xuan Liu,Yu Ting He,Ling Liang,Liu Fu Hu,Yue Liu,Rui-xing Yu,Bo Zhi Chen,Yong Cui,Xin Dong Guo 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

In this study, we reported two types of PMNs based on polylactic acid (PLA) and polyvinyl alcohol (PVA),respectively. Parafilm M film, porcine skin, and rats’ models were operated to evaluate the mechanicalproperties in vitro and in vivo to find optimal parameters for efficient insertion. Insertion depth was measuredusing Digital Force Gauge by changing insertion force and speed, respectively. Results showed thatincreasing the insertion force and speed used for PMNs application led to a significant increase in thedepth of insertion. A force of 18 N under a speed of 330 mm/min was the optimal condition for insertingPMNs array into ParafilmM film and porcine skin. In addition, PLA-MNs exhibited higher robustness andenhanced homogeneity in insertion depth compared with PVA-MNs, but PVA-MNs were able to reachmuch deeper insertion depth. Moreover, Sprague Dawley (SD) rat experiments confirmed the effectivenessof optimal insertion parameters for transdermal drug delivery. This study illustrated not only thedevelopment of novel PMNs but also the mechanical evaluation for the design of PMNs.

Erratum to: Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells

( Rui-dong Li ),( Zhong-liang Deng ),( Ning Hu ),( Xi Liang ),( Bo Liu ),( Jinyong Luo ),( Liang Chen ),( Liangjun Yin ),( Xiaoji Luo ),( Wei Shui ),( Tong-chuan He ),( Wei Huang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.5

Estimation of frost durability of recycled aggregate concrete by hybridized Random Forests algorithms

Rui Liang,Behzad Bayrami 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.49 No.1

An effective approach to promoting sustainability within the construction industry is the use of recycled aggregate concrete ( ) as a substitute for natural aggregates. Ensuring the frost resilience of technologies is crucial to facilitate their adoption in regions characterized by cold temperatures. The main aim of this study was to use the Random Forests ( ) approach to forecast the frost durability of in cold locations, with a focus on the durability factor ( ) value. Herein, three optimization algorithms named Sine-cosine optimization algorithm ( ), Black widow optimization algorithm ( ), and Equilibrium optimizer ( ) were considered for determing optimal values of hyperparameters. The findings show that all developed systems faithfully represented the , with an 2 for the train and test data phases of better than 0.9539 and 0.9777, respectively. In two assessment and learning stages, − is found to be superior than − and − . The outperformed model's performance ( − ) was superior to that of (from literature) by raising the values of 2 and reducing the values. Considering the justifications, as well as the comparisons from metrics and Taylor diagram’s findings, it could be found out that, although other models were equally reliable in predicting the the frost durability of based on the durability factor ( ) value in cold climates, the developed − strategy excelled them all.

Cold hardiness characteristic of the overwintering pupae of fall webworm Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) in the northeast of China

Xiao-Rui Xu,Ming-Ming Zhu,Liang-Liang Li,Guang-Cai Zhang,Ye Zheng,Ting Li,Shou-Hui Sun 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.1

The fallwebworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), is an invasive and important pest in China. Understanding the physiological–biochemical characteristics of the overwintering pupae is essential for its cold tolerance and potential distribution in the northeast of China. Results indicated that the supercooling point (SCP) of H. cunea overwintering pupae varied significantly among Jilin city (43°N, 126°E), Jilin Province, Shenyang (41.8°N, 123.4°E), Liaoning Province and Yingkou (40.39°N, 122.13°E), Liaoning Province. The average SCP in Jilin was the lowest (−23.49 ± 0.46 °C) in those of three areas. The water content in overwintering pupae had a significant positive correlation with the SCP (R = 0.848,P = 0.03). Contents of alanine (ALA) (26.00%), lysine (LYS) (30.51%) and arginine (ARG) (22.88%) in the overwintering pupae's hemolymph were higher than others. During the overwintering, the fat content in pupae's bodies decreased gradually. Analysis of variance (ANOVA) indicated that the content of trehalose in the body of H. cunea was remarkably different (P= 0.007) month by month, with the maximum in March (2.88 mg·mL−1) and the minimum (0.40 mg·mL−1) in November. Pearson's correlation test indicated that SCP had a significant negative correlation with the SOD enzyme activity (R = −0.850; P = 0.008). Thermal hysteresis activity (THA) of hemolymph was 0.68°C and 0.7–0.8°C for H. cunea pupae and larva, respectively, using differential scanning calorimetry, which suggests that the H. cunea can improve their cold tolerance through producing antifreeze proteins. These results could help us to predict the distribution of the H. cunea in the northeast of China.

Radix Tetrastigma Hemsleyani Flavone Induces Apoptosis in Human Lung Carcinoma A549 Cells by Modulating the MAPK Pathway

Zhong, Liang-Rui,Chen, Xian,Wei, Ke-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Radix Tetrastigma Hemsleyani Flavone (RTHF) is widely used as a traditional herb for its detoxification and anti-inflammation activity. Recently, several studies have shown that RTHF can inhibit growth and induce apoptosis in human cancer cell lines. However, the mechanisms are not completely understood yet. In this study we investigated the potential effects of RTHF on growth and apoptosis in human lung adenocarcinoma A549 cells as well as its mechanisms. A549 cells were treated with RTHF at various concentrations for different times. In vitro the MTT assay showed that RTHF had obvious anti-proliferation effects on A549 cells in a dose- and time-dependent manner. Cell morphological changes observed by inverted microscope and Hoechst33258 methods were compared with apoptotic changes observed by fluorescence microscope. Cell apoptosis inspected by flow cytometry showed significant increase in the treatment group over the control group (P<0.01). Expression of apoptosis related Bax/Bcl-2, caspases and MAPK pathway proteins were detected by Western blotting. The results showed that RTHF up-regulated the Bax/Bcl-2 ratio and cle-caspase3/9, cle-PARP expression in a dose-dependent manner. Expression of p-p38 increased, p-ERK decreased significantly and that of p-JNK was little changed in the RTHF group when compared with the control group. These results suggest that RTHF might exert anti-growth and apoptosis activity against lung cancer A549 cells through activation of caspases and Bcl-2 family proteins and the MAPK pathway, therefore presenting as a promising therapeutic agent for the treatment of lung cancer.