Breviscapine Ameliorates Fear Extinction and Anxiety in BALB/cJ Mice

Wei Liang(Wei Liang),Jing Huang(Jing Huang),Shuya Yang(Shuya Yang),Peng Huang(Peng Huang),Jun Chen(Jun Chen),Chen Chen(Chen Chen),Qun Yang(Qun Yang),Fengzhan Li(Fengzhan Li) 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.3

Objective In this research, the influence of breviscapine on anxiety, fear elimination, and aggression and the potential mechanism was investigated. Methods Anxiety and locomotion were analyzed by elevated plus maze and open field test in mice. Bussey-Saksida Mouse Touch Screen Chambers were used to perform fear conditioning. Territorial aggression was assessed by resident intruder test. Protein levels were evaluated by Western blot. Breviscapine improved fear-extinction learning in BALB/cJ mice. Results Breviscapine at 20-100 mg/kg increased center cross number, total distance traveled, and velocity in a dose-dependent manner. On the other hand, breviscapine at 20-100 mg/kg decreased the immobility time in open field test. In addition, breviscapine at 20-100 mg/kg increased the ratio of time on the open arm, time on the distal parts of the open arm, and total distance traveled in elevated plus maze. Breviscapine at 100 mg/kg increased the average attack latency and decreased the number of attacks over the last 3 days of resident intruder test. In hippocampus, protein levels of postsynaptic density protein-95 and synaptophysin were elevated by breviscapine at these three doses. Conclusion The administration of breviscapine alleviates fear extinction, anxiety, and aggression, while increases locomotor in a dose-dependent manner, which might be associated with its influence on synaptic function.

Localized surface plasmon resonance enhanced by the light-scattering property of silver nanoparticles for improved luminescence of polymer light-emitting diodes

Cheng-Liang Huang,Hung Ji Huang,Sy-Hann Chen,Yu-Siang Huang,Po-Ching Kao,Yuan-Fong Chou Chau,Hai-Pang Chiang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

This study used photochemical reduction to successfully synthesize triangular silver nanoplates (TAgNPs)and silver nanodecahedrons (AgNDs) with higher light-absorption and higher light-scattering properties,respectively, for the same wavelength. To analyze the contribution of light-absorption and lightscatteringof silver nanoparticles to the localized surface plasmon resonance (LSPR) effect, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate was doped with TAgNPs or AgNDs at the same concentration(1.17 lg/cm2) and made into polymer light-emitting diodes (PLEDs). According to the current densityvoltageluminancecharacteristics and electroluminescence (EL) spectra, the enhancement factors for currentefficiency and EL intensity for AgND-containing PLEDs were found to be higher than those for PLEDwith TAgNPs by 24.9% and 138%, respectively. This shows that the metal nanoparticles with higher lightscatteringproperty can induce a relatively strong LSPR effect, which possibly gives a hint to design plasmonicphotovoltaic in future.

Computer Network Vulnerability Assessment and Safety Evaluation Application based on Bayesian Theory

Liang Huang,Xinhao Chen,Xinsheng Lai 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.12

Computer network vulnerability analysis is a method of analysis and evaluation of network security beforehand. The attacks method has occurred in the network, the previous network status change as input information, calculated by the model analysis. Forecasting network node may be network attacks given the current security level value network, network security reinforcement measures taken before the danger. Administrators can proactively identify network security issues, to take measures in advance to avoid information leakage, financial losses, ensure the safety of individuals and countries. Therefore, vulnerability analysis computer network is very important. Based on the properties of attack graph shows the method of attack graphs to Bayesian network transformation, using the new algorithm to eliminate loops attribute attack graph optimization, building the Bayesian attribute attack graph model used to evaluate the network itself security situation. In this model, based on Bayes formula for calculating the probability of a new node probability calculation formula and attack paths occur for calculating network vulnerability assessment of the quantitative indicators. The model not only can visually process description of cyber attacks, but also into the Bayesian network probabilistic thinking of possible network attack path prediction and assessment.

Truncated Kernel Projection Machine for Link Prediction

Huang, Liang,Li, Ruixuan,Chen, Hong Korean Institute of Information Scientists and Eng 2016 Journal of Computing Science and Engineering Vol.10 No.2

With the large amount of complex network data that is increasingly available on the Web, link prediction has become a popular data-mining research field. The focus of this paper is on a link-prediction task that can be formulated as a binary classification problem in complex networks. To solve this link-prediction problem, a sparse-classification algorithm called "Truncated Kernel Projection Machine" that is based on empirical-feature selection is proposed. The proposed algorithm is a novel way to achieve a realization of sparse empirical-feature-based learning that is different from those of the regularized kernel-projection machines. The algorithm is more appealing than those of the previous outstanding learning machines since it can be computed efficiently, and it is also implemented easily and stably during the link-prediction task. The algorithm is applied here for link-prediction tasks in different complex networks, and an investigation of several classification algorithms was performed for comparison. The experimental results show that the proposed algorithm outperformed the compared algorithms in several key indices with a smaller number of test errors and greater stability.

Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells

Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

Probing two-dimensional metallic-like and localization effects at low magnetic fields

Huang, T.Y.,Liang, C.T.,Kim, G.H.,Huang, C.F.,Huang, C.P.,Ritchie, D.A. North-Holland 2010 Physica E, Low-dimensional systems & nanostructure Vol.42 No.4

The metallic-like regime characterized by Shubnikov-de Haas (SdH) oscillations is investigated between localization-induced weak insulator and quantum Hall liquid in a gated two-dimensional GaAs electron system containing InAs dots. Multiple SdH crossing points are observed in the longitudinal resistivity before the appearance of the critical point of a plateau transition with increasing perpendicular magnetic field. In conductivities, however, there is no corresponding crossing in the metallic-like regime because of the T-dependent Hall slope under electron-electron interaction, which provides an explicit way to distinguish SdH crossing points from the critical point in our study.

Breviscapine Ameliorates Fear Extinction and Anxiety in BALB/cJ Mice

Liang Wei,Huang Jing,Yang Shuya,Huang Peng,Chen Jun,Chen Chen,Yang Qun,Li Fengzhan 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.4

Objective In this research, the influence of breviscapine on anxiety, fear elimination, and aggression and the potential mechanism was investigated.Methods Anxiety and locomotion were analyzed by elevated plus maze and open field test in mice. Bussey-Saksida Mouse Touch Screen Chambers were used to perform fear conditioning. Territorial aggression was assessed by resident intruder test. Protein levels were evaluated by Western blot. Breviscapine improved fear-extinction learning in BALB/cJ mice.Results Breviscapine at 20–100 mg/kg increased center cross number, total distance traveled, and velocity in a dose-dependent manner. On the other hand, breviscapine at 20–100 mg/kg decreased the immobility time in open field test. In addition, breviscapine at 20–100 mg/kg increased the ratio of time on the open arm, time on the distal parts of the open arm, and total distance traveled in elevated plus maze. Breviscapine at 100 mg/kg increased the average attack latency and decreased the number of attacks over the last 3 days of resident intruder test. In hippocampus, protein levels of postsynaptic density protein-95 and synaptophysin were elevated by breviscapine at these three doses.Conclusion The administration of breviscapine alleviates fear extinction, anxiety, and aggression, while increases locomotor in a dose-dependent manner, which might be associated with its influence on synaptic function.

High-Performance Bottom-Contact Organic Thin-Film Transistors Based on Benzo[<i>d</i>,<i>d</i>′]thieno[3,2-<i>b</i>;4,5-<i>b</i>′]dithiophenes (BTDTs) Derivatives

Huang, Peng-Yi,Chen, Liang-Hsiang,Kim, Choongik,Chang, Hsiu-Chieh,Liang, You-jhih,Feng, Chieh-Yuan,Yeh, Chia-Ming,Ho, Jia-Chong,Lee, Cheng-Chung,Chen, Ming-Chou American Chemical Society 2012 ACS APPLIED MATERIALS & INTERFACES Vol.4 No.12

<P>Three benzo[<I>d</I>,<I>d</I>′]thieno[3,2-<I>b</I>;4,5-<I>b</I>′]dithiophene (<B>BTDT</B>) derivatives, end-functionalized with benzothiophenyl (<B>BT-BTDT</B>; <B>2</B>), benzothieno[3,2-b]thiophenyl (<B>BTT-BTDT</B>; 3), and benzo[<I>d</I>,<I>d</I>′]thieno[3,2-<I>b</I>;4,5-<I>b</I>′]dithiophenyl (<B>BBTDT</B>; <B>4</B>), were prepared for bottom-contact/bottom-gate organic thin-film transistors (OTFTs). An improved one-pot [2 + 1 + 1] synthetic method of <B>BTDT</B> with improved synthetic yield was achieved, which enabled the efficient realization of new <B>BTDT</B>-based semiconductors. All of the <B>BTDT</B> compounds exhibited high performance p-channel characteristics with carrier mobilities as high as 0.34 cm<SUP>2</SUP>/(V s) and a current on/off ratio of 1 × 10<SUP>7</SUP>, as well as enhanced ambient stability. The device characteristics have been correlated with the film morphologies and microstructures of the corresponding compounds.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2012/aamick.2012.4.issue-12/am3022448/production/images/medium/am-2012-022448_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am3022448'>ACS Electronic Supporting Info</A></P>

Potential Biomarker of L-type Amino Acid Transporter 1 in Breast Cancer Progression

Liang, Zhongxing,Cho, Heidi T.,Williams, Larry,Zhu, Aizhi,Liang, Ke,Huang, Ke,Wu, Hui,Jiang, Chunsu,Hong, Samuel,Crowe, Ronald,Goodman, Mark M.,Shim, Hyun-Suk The Korea Society of Nuclear Medicine 2011 핵의학 분자영상 Vol.45 No.2

Purpose L-type amino acid transporter 1 (LAT1) is essential for the transport of large neutral amino acids. However, its role in breast cancer growth remains largely unknown. The purpose of the study is to investigate whether LAT1 is a potential biomarker for the diagnosis and treatment of breast cancer. Methods LAT1 mRNA and protein levels in breast cancer cell lines and tissues were analyzed. In addition, the effects of targeting LAT1 for the inhibition of breast cancer cell tumorigenesis were assessed with soft agar assay. The imaging of xenograft with anti-1-amino-3-[$^{18}F$]fluorocyclobutane-1-carboxylic acid (anti-[$^{18}F$]FACBC) PET was assessed for its diagnostic biomarker potential. Results Normal breast tissue or low malignant cell lines expressed low levels of LAT1 mRNA and protein, while highly malignant cancer cell lines and high-grade breast cancer tissue expressed high levels of LAT1. In addition, higher expression levels of LAT1 in breast cancer tissues were consistent with advanced-stage breast cancer. Furthermore, the blockade of LAT1 with its inhibitor, 2-aminobicyclo[ 2.2.1]heptane-2-carboxylic acid (BCH), or the knockdown of LAT1 with siRNA, inhibited proliferation and tumorigenesis of breast cancer cells. A leucine analog, anti-[$^{18}F$]FACBC, has been demonstrated to be an excellent PET tracer for the non-invasive imaging of malignant breast cancer using an orthotopic animal model. Conclusions The overexpression of LAT1 is required for the progression of breast cancer. LAT1 represents a potential biomarker for therapy and diagnosis of breast cancer. Anti-[$^{18}F$]FACBC that correlates with LAT1 function is a potential PET tracer for malignant breast tumor imaging.

Checking Relationship Consistency and Class Redundancy in a Class Diagram under Model-Driven Engineering

Liang Huang,Yucong Duan,Honghao Gao,Hui Li,Caimao Li,Zhiyang Lin 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.10

Model-Driven Engineering (MDE) tries to reduce the effort spent on software development by generating codes from models. People concentrate their minds on the transformation between models and models, or between models and codes. People also concentrate on checking consistency between different models such as consistency between a class diagram and a sequence diagram and consistency between a sequence diagram and a state machine diagram. Checking relationship consistency and class redundancy in a class model is still important but ignored in recent years. This paper concentrates on relationship problems between classes in a class diagram and proposes methods of checking various relationship problems. We address the redundancy of a class’s operations and attributes. We identify a large range of the problems for class diagram. Our research is based on the relationship abstraction rules.