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Gilang Gumilar,Ni Luh Wulan Septiani,Shofarul Wustoni,Taufik Ramdani Tohari,Wahyu Widayat,Muhammad Yusuf,Hesti L. Wiraswati,Lia Faridah,Savira Ekawardhani,Isa Anshori,Brian Yuliarto 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has become a dangeroushuman pathogen and caused severe illness that can lead to death. Yet, the standard practical diagnostictools for detecting the SARS-CoV-2 virus require multi-step assay, including extraction and labeling steps. Thus, a simple and accurate detection tool is incredibly desirable to support on-site screening, preventingits spread. In this work, we developed a portable surface plasmon resonance (SPR) sensor chip modifiedwith metal–organic frameworks (MOF) UiO-66 that serves as an immobilization matrix of single-chainvariable fragment (scFv) receptors. Large surface area and good adsorption of UiO-66/scFv-modified surfacelead to an improvement of binding capacity towards the protein target of the SARS-CoV-2 receptorbinding domain (RBD). We further successfully demonstrated the use of this portable SPR to detect theSARS-CoV-2 virus from patients’ nasopharyngeal swab samples. Interestingly, the SPR sensors candirectly distinguish the positive and negative patients within 15 minutes on 23 samples, simultaneously,without the need for labeling, and the results are in line with the gold standard PCR tests. In addition, thedetection limit of the sensors is 10.085 105 virus/mL, which follows the standard of practical screeningtools. Therefore, these UiO-66 modified SPR chip sensors offer a rapid, label-free, and low-cost detectionof the SARS-CoV-2 virus that can be potentially developed for other infectious diseases.