Characteristics of isolated lactic acid bacteria and their effects on the silage quality

Siran Wang,Xianjun Yuan,Zhihao Dong,Junfeng Li,Gang Guo,Yunfeng Bai,Junyu Zhang,Tao Shao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures (10°C, 15°C, and 25°C). Results: All isolated strains (LCG3, LTG7, I5, and LI3) could grow at 5°C to 20°C, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/ total nitrogen (AN/TN) compared with uninoculated silages at various temperatures (10°C, 15°C, and 25°C). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at 10°C and 15°C, indicated by similar pH, LA content and AN/TN. Conclusion: All inoculants could improve the silage fermentation quality at various temperatures (10°C, 15°C, and 25°C). At the temperature of 10°C and 15°C, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.

Facile and controllable synthesis of nitrogen self-doped chitosan-derived carbon for high-performance Li-ion batteries

Xia Wentao,Cheng Miao,Hu Jing,Liu Qianqian,Wei Tao,Wang Ruirui,Li Wanfei,Liu Bo 한국탄소학회 2024 Carbon Letters Vol.34 No.1

N-doping content and configurations have a significant effect on the electrochemical performance of carbon anodes. Herein, we proposed a simple method to synthesize highly N self-doped chitosan-derived carbon with controllable N-doping types by introducing 2ZnCO3·3Zn(OH)2 into the precursor. The as-synthesized NC-CS/2ZnCO3·3Zn(OH)2 electrode exhibited more than twice the reversible capacity (518 mAh g−1 after 100 cycles at 200 mA g−1) compared to the NC-CS electrode, superior rate performance and outstanding cycling stability. The remarkable improvement should be mainly attributed to the increase of N-doping content (particularly the pyrrolic-N content), which provided more active sites and favored Li+ diffusion kinetics. This study develops a cost-effective and facile synthesis route to fabricate high-performance N self-doped carbon with tunable doping sites for rechargeable battery applications.

Evolution of the effect of sulfur confinement in graphene-based porous carbons for use in Li-S batteries

Jia, Xiangling,Zhang, Chen,Liu, Juanjuan,Lv, Wei,Wang, Da-Wei,Tao, Ying,Li, Zhengjie,Zheng, Xiaoyu,Yu, Jong-Sung,Yang, Quan-Hong The Royal Society of Chemistry 2016 Nanoscale Vol.8 No.8

<P>A controllable drying strategy is proposed for the precise and non-destructive control over the structure of a 3D graphene assembly. Such an assembly is used as a model carbon material to investigate the pore structure-dependent shuttle effect and cycling performance of the cathode of a Li-S battery.</P>

Superhydrophobic ceramic hollow fiber membrane planted by ZnO nanorod-array for high-salinity water desalination

Wang, Tao,Yun, Yanbin,Wang, Manxiang,Li, Chunli,Liu, Guicheng,Yang, Woochul Elsevier 2019 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.105 No.-

<P><B>Abstract</B></P> <P>To solve the problems of membrane material tolerance and membrane fouling, a micro/nano hierarchial structure with low surface energy was constructed above the exterior surface of a ceramic hollow fiber membrane using ZnO nanorod arrays and 1H,1H,2H,2H-perfluorodecyltriethoxysilane (PDTS) coatings, which make it superhydrophobic and self-cleaning. The surface morphology, chemical functional groups, and water contact angel of the modified membranes were identified. The results show that large quantity of ZnO nanorods possess desirable characteristics (<I>i.e.</I> superhydrophobicity, exceptional thermal and mechanical stability, and water contact angle of 160.12°) were detected on the ceramic membrane. The novel membrane shows excellent self-cleaning performance and good desalination ability in the utilization of vacuum membrane distillation (VMD) system for high-salinity water desalination.</P> <P><B>Highlights</B></P> <P> <UL> <LI> • Ceramic membrane is modified by imitating of superhydrophobic biological surfaces. </LI> <LI> • The micro/nano hierarchical structure with low surface energy endows the superhydrophobicity. </LI> <LI> • The P-ZN-CHF membrane obtains antifouling ability. </LI> <LI> • The modified membrane shows excellent performances during vacuum membrane distillation. </LI> </UL> </P>

Comparative transcriptome analysis reveals differential transcription in heat-susceptible and heat-tolerant pepper (Capsicum annum L.) cultivars under heat stress

Tao Li,Xiaowan Xu,Ying Li,Hengming Wang,Zhiliang Li,Zhenxing Li 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6

Heat stress is an important agricultural problem around the world. In pepper (Capsicum annum L.), heat stress seriously affects pollination and yield. However, to date, the molecular basis of heat stress has not been extensively studied. Using the HiSeq™ 2000 sequencing platform, the seedling transcriptome of heat-susceptible C.annuum ‘S590’(CaS) and heat-tolerant ‘R597’ (CaR) under the heat stress was examined. Over five million clean reads were generated from each library, each corresponding to a coverage of >250,000 nt. About 73% of the reads were mapped to the pepper genome, and 3,799 and 4,010 differentially expressed genes (DEGs) were identified in ‘R597’ (CaR) and ‘S590’(CaS), respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses determined that the identified DEGs were involved in heat shock protein, heat shock transcription factors, hormone, as well as calcium and kinase signaling. Further validation identified 35 genes that were involved in stress response, and that most of the heat shock proteins were upregulated in two genotypes, and highly expressed in susceptible S590 than in tolerant cultivar R597; the transcription factors and hormone signaling genes showed higher levels of expression in the heat-tolerant cultivar R597 than that observed in the heat-susceptible S590. These findings facilitate in better understanding of the molecular mechanism underlying heat stress in different pepper genotypes.

Molecular and functional characterization of the adiponectin (AdipoQ) gene in goat skeletal muscle satellite cells

Wang, Linjie,Xue, Ke,Wang, Yan,Niu, Lili,Li, Li,Zhong, Tao,Guo, Jiazhong,Feng, Jing,Song, Tianzeng,Zhang, Hongping Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: It is commonly accepted that adiponectin binds to its two receptors to regulate fatty acid metabolism in adipocytes. To better understand their functions in the regulation of intramuscular adipogenesis in goats, we cloned the three genes (adiponectin [AdipoQ], adiponectin receptor 1 [AdipoR1], and AdipoR2) encoding these proteins and detected their mRNA distribution in different tissues. We also determined the role of AdipoQ in the adipogenic differentiation of goat skeletal muscle satellite cells (SMSCs). Methods: SMSCs were isolated using 1 mg/mL Pronase E from the longissimus dorsi muscles of 3-day-old female Nanjiang brown goats. Adipogenic differentiation was induced in satellite cells by transferring the cells to Dulbecco's modified Eagle's medium supplemented with an isobutylmethylxanthine, dexamethasone and insulin cocktail. The pEGFP-N1-AD plasmid was transfected into SMSCs using Lipofectamine 2000. Expression of adiponectin in tissues and SMSCs was detected by quantitative polymerase chain reaction and immunocytochemical staining. Results: The three genes were predominantly expressed in adipose and skeletal muscle tissues. According to fluorescence and immunocytochemical analyses, adiponectin protein expression was only observed in the cytoplasm, suggesting that adiponectin is localized to the cytoplasm of goat SMSCs. In SMSCs overexpressing the AdipoQ gene, adiponectin promoted SMSC differentiation into adipocytes and significantly (p<0.05) up-regulated expression of AdipoR2, acetyl-CoA carboxylase, fatty-acid synthase, and sterol regulatory element-binding protein-1, though expression of CCAAT/enhancer-binding $protein-{\alpha}$, peroxisome proliferator-activated receptor ${\gamma}$, and AdipoR1 did not change significantly. Conclusion: Adiponectin induced SMSC differentiation into adipocytes, indicating that adiponectin may promote intramuscular adipogenesis in goat SMSC.

Synthesis, characterization, and temperature-dependent colloidal stability of poly(N-isopropylacrylamide)-grafted polystyrene/poly(styrene-co-4-vinylbenzyl N, N-diethyldithiocarbamate) hairy particles

Wang, Chun,Wang, Tao,Li, Li,Huh, Kang Moo,Shi, Shan,Kuroda, Shin-ichi Springer-Verlag 2012 Colloid and polymer science Vol.290 No.13

Prediction Models for Solitary Pulmonary Nodules Based on Curvelet Textural Features and Clinical Parameters

Wang, Jing-Jing,Wu, Hai-Feng,Sun, Tao,Li, Xia,Wang, Wei,Tao, Li-Xin,Huo, Da,Lv, Ping-Xin,He, Wen,Guo, Xiu-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Lung cancer, one of the leading causes of cancer-related deaths, usually appears as solitary pulmonary nodules (SPNs) which are hard to diagnose using the naked eye. In this paper, curvelet-based textural features and clinical parameters are used with three prediction models [a multilevel model, a least absolute shrinkage and selection operator (LASSO) regression method, and a support vector machine (SVM)] to improve the diagnosis of benign and malignant SPNs. Dimensionality reduction of the original curvelet-based textural features was achieved using principal component analysis. In addition, non-conditional logistical regression was used to find clinical predictors among demographic parameters and morphological features. The results showed that, combined with 11 clinical predictors, the accuracy rates using 12 principal components were higher than those using the original curvelet-based textural features. To evaluate the models, 10-fold cross validation and back substitution were applied. The results obtained, respectively, were 0.8549 and 0.9221 for the LASSO method, 0.9443 and 0.9831 for SVM, and 0.8722 and 0.9722 for the multilevel model. All in all, it was found that using curvelet-based textural features after dimensionality reduction and using clinical predictors, the highest accuracy rate was achieved with SVM. The method may be used as an auxiliary tool to differentiate between benign and malignant SPNs in CT images.

Elevated Mean Platelet Volume is Associated with Presence of Colon Cancer

Li, Jia-Ying,Li, Ying,Jiang, Zheng,Wang, Rui-Tao,Wang, Xi-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Colon cancer is the second most common cancer in developed countries. Activated platelets play a key role in inflammation and atherothrombosis, with mean platelet volume (MPV) is an early marker of platelet activation. The aim of the study was to clarify the relevance of MPV in patients with colon cancer. Materials and Methods: We measured MPV levels in 128 patients with colon cancer before and after surgery, and 128 controls matched for age, gender, body mass index (BMI) and smoking status. The odds ratios (ORs) and 95% confidence intervals (CIs) for colon cancer were calculated using multivariate logistic regression analyses across MPV quartiles. Results: Patients with colon cancer had higher MPV compared with controls. Surgical tumor resection resulted in a significant decrease in MPV levels (11.4 fL vs 10.7 fL; p<0.001). A positive correlation between MPV and tumor-nodule-metastases (TNM) stage was found. Furthermore, after adjusting for other risk factors, the ORs (95%CIs) for colon cancer according to MPV quartiles were 1.000, 2.238 (1.014-4.943), 3.410 (1.528-7.613), and 5.379 (2.372-12.198), respectively. Conclusions: The findings show that patients with colon cancer have higher MPV levels compared with controls, and these are reduced after surgery. In addition, MPV was found to be independently associated with the presence of colon cancer. Further studies are warranted to assess the utility of MPV as a novel diagnostic screening tool for colon cancer.

EFFECTS OF DILUTION COMBUSTION AND MILLER CYCLE ON THE PERFORMANCE OF GASOLINE ENGINE

Tao Wang,Zhaoming Huang,Li Wang,Kai Shen 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.2

Dilution and Miller cycle are effective ways to reduce fuel consumption. Based in a 4-cylinder, 4-stroke, turbocharged, low pressure EGR, in-cylinder direct injection gasoline engine, with two intake camshaft angle (133 °CA, 170 °CA ), the effects of dilution (lean combustion, EGR) and Miller cycle on combustion and fuel economy at the minimum fuel consumption (2750 rpm, 11 bar) were studied. Besides, 1D engine simulation and 3D flow simulation are used to study the difference of combustion modes. When COVimep is less than 3 %, the BSFC of EGR is lower than that of lean combustion at the same dilution rate. The dilution limit of lean combustion is larger than EGR. When the dilution rates of EGR and lean combustion are 22 % and 35.9 %, respectively, the BSFC reaches the minimum, which decreases by 4.7 % and 7.2 % respectively. The specific heats ratio of lean combustion is larger than EGR, so the theoretical thermal efficiency of lean combustion is higher. Miller cycle reduces intake time and intake valve lift, which result in the tumble ratio and turbulent kinetic energy decrease significantly. EGR has greater fuel-saving potential for Otto cycle engine than Miller cycle.