Effects of reactive oxidants generation and capacitance on photoelectrochemical water disinfection with self-doped titanium dioxide nanotube arrays

Cho, Kangwoo,Lee, Seonggeun,Kim, Hyeonjeong,Kim, Hyung-Eun,Son, Aseom,Kim, Eun-ju,Li, Mengkai,Qiang, Zhimin,Hong, Seok Won Elsevier BV 2019 Applied Catalysis B Vol.257 No.-

<P><B>Abstract</B></P> <P>We herein provide photoelectrochemical (PEC) disinfection activities of anodically prepared TiO<SUB>2</SUB> nanotube (TNT) arrays (diameter ˜ 100 nm, length ˜ 16 μm on average) that were electrochemically self-doped before (bk-TNT) and after (bl-TNT) an atmospheric annealing at 450 °C. The X-ray diffraction indicated predominating anatase TiO<SUB>2</SUB> signal on bl-TNT, while substantial lattice distortion was noticed for bk-TNT. Although the X-ray photoelectron spectra indicated negligible Ti<SUP>3+</SUP> on surface of both TNTs, linear sweep (cyclic) voltammetry and electrochemical impedance spectrometry confirmed the bk-TNT to show greater double layer capacitance and overall photocurrent, coupled with lower charge transfer resistance. Nevertheless, the PEC disinfection of <I>E. coli</I> was significantly invigorated on bl-TNT, while the bactericidal rates in tap water were comparable or even far greater than those in 0.1 M Na<SUB>2</SUB>SO<SUB>4</SUB> solutions, depending on [<I>E. coli</I>]<SUB>0</SUB> (10<SUP>5</SUP> or 10<SUP>7</SUP> CFU/mL). Under a presumed diffusion-controlled kinetic regime in this study, observed effects of capacitance and electrolyte could be interpreted in terms of electrostatic interaction between the electrical double layer of photoanodes and charged bio-solids, such as repulsion by co-ions (SO<SUB>4</SUB> <SUP>2−</SUP>) and adsorption/surface blocking. Analogous PEC experiments on model organic compounds degradation (4-chlorophenol and methylene blue) corroborated a long-term stability of the bl-TNT (up to 30 consecutive cycles) and the role of surface hydroxyl radical as the primary oxidant.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Self-doped anatase (bl-TNT) and amorphous TiO<SUB>2</SUB> nanotube (bk-TNT) were interrogated. </LI> <LI> Lower double layer capacitance of bl-TNT led to higher PEC disinfection of <I>E. coli</I>. </LI> <LI> PEC bactericidal rates in tap water were similar or greater than in Na<SUB>2</SUB>SO<SUB>4</SUB> solution. </LI> <LI> Electrostatic interaction between photoanode and bio-solids determined the kinetics. </LI> <LI> Commercial applicability for point-of-use disinfection was demonstrated. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A Comparative Study on Physiological Activities of Lager and Ale Brewing Yeasts under Different Gravity Conditions

Zhimin Yu,Mou-Ming Zhao,Huiping Li,Hai-Feng Zhao,Qingli Zhang,Chunyan Wan,Huipin Li 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4

High gravity (HG) or very high gravity (VHG)brewing has become popular in modern breweries due to its economic and product quality advantages. However,there are the negative impacts such as the fermentation performance of brewer’s yeast in HG or VHG wort, which are closely related to changes in cell physiological activity. In the present study, 3 kinds of worts, with different gravities, were used to examine the systematic effects on fermentation performance and physiological activity of lager yeast FBY009505 (Saccharomyces pastorianus) and ale yeast FBY0099 (Saccharomyces cerevisiae), as well as the resulting beer flavor. Results showed that the responses of FBY009505 and FBY0099 to the HG or VHG worts were similar. The specific fermentation rate and viability of cropped yeast of FBY009505 and FBY0099 were decreased with increasing wort gravity. The increased wort gravity resulted in the increase of energy charge and the decrease of α-glucosides transport rate and glycolytic enzyme activities. Moreover, the environmental stresses in the HG or VHG wort showed a higher inhibitory activity against α-glucoside transport than glycolytic enzymes. The content of intracellular trehalose and glycerol of FBY009505 and FBY0099 increased with the increase in wort gravity. The results from this study provided a potential means to systematically understand the physiology of brewer’s yeast under HG or VHG conditions. High gravity (HG) or very high gravity (VHG)brewing has become popular in modern breweries due to its economic and product quality advantages. However,there are the negative impacts such as the fermentation performance of brewer’s yeast in HG or VHG wort, which are closely related to changes in cell physiological activity. In the present study, 3 kinds of worts, with different gravities, were used to examine the systematic effects on fermentation performance and physiological activity of lager yeast FBY009505 (Saccharomyces pastorianus) and ale yeast FBY0099 (Saccharomyces cerevisiae), as well as the resulting beer flavor. Results showed that the responses of FBY009505 and FBY0099 to the HG or VHG worts were similar. The specific fermentation rate and viability of cropped yeast of FBY009505 and FBY0099 were decreased with increasing wort gravity. The increased wort gravity resulted in the increase of energy charge and the decrease of α-glucosides transport rate and glycolytic enzyme activities. Moreover, the environmental stresses in the HG or VHG wort showed a higher inhibitory activity against α-glucoside transport than glycolytic enzymes. The content of intracellular trehalose and glycerol of FBY009505 and FBY0099 increased with the increase in wort gravity. The results from this study provided a potential means to systematically understand the physiology of brewer’s yeast under HG or VHG conditions.

A 14 Bit Continuous-Time Delta-Sigma A/D Modulator With 2.5 MHz Signal Bandwidth

Li, Zhimin,Fiez, Terri S. IEEE 2007 IEEE journal of solid-state circuits Vol.42 No.9

<P> A continuous-time delta-sigma A/D modulator with 5 MS/s output rate in a 2.5 V 0.25 <TEX>$\mu$</TEX>m CMOS process is presented. The modulator has a fifth-order single-stage, dual-loop architecture allowing nearly one clock period quantizer delay. A multi-bit quantizer is used to increase resolution and multi-bit non-return-to-zero DACs are adopted to reduce clock jitter sensitivity. Capacitor tuning is utilized to overcome loop coefficient shifts due to process variations. Self-calibration is implemented to suppress current-steering DAC mismatch. Clocked at 60 MHz, the prototype chip achieves 81 dB peak SNR and 85 dB dynamic range with a 12X oversampling ratio. The power consumption is 50 mW. </P>

Characterization of Rosa chinensis CBF Genes and the Function of RcCBF6 in Cold Tolerance

Jianbo Li,Guangshun Zheng,Shaofeng Li,Zhimin Tang,Jingshuang Sun,Xuebing Xin 한국식물학회 2020 Journal of Plant Biology Vol.63 No.4

As a class of AP2 family transcription factors, C-repeat binding factor (CBF) gene family plays an important role in resisting low-temperature stress and improving cold tolerance of plants. Rosa chinensis is an important horticultural and ornamental plant, but little is known about the molecular characteristics of CBF genes in R. chinensis. In our study, six RcCBFs members were identified at R. chinensis whole genome, including one member RcCBF1 on Chromosome 1 and other five members RcCBF2-6 on Chromosome 7. The analysis of cis-acting elements indicated that there were various cis-acting elements related to stress, development, hormone, and light. The expression profiles showed that most of RcCBFs were mainly expressed in root, and the expression levels of RcCBFs were significantly induced by low-temperature stress, especially RcCBF6. To verify the function of RcCBF6, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The RcCBF6-overexpressing plants exhibited higher tolerance to cold stress as evidenced by a better growth and higher antioxidative enzyme activities than the wild-type plants. Furthermore, the expression levels of some cold-response genes were up-regulated in the transgenic plants, such as KIN1, RD29A, LTP3, and GOLS3. Our study contributes to a better understanding of RcCBF gene family and provides a foundation for the further functional research of RcCBFs.

UPSKILLING FOR FASHION DESIGN INNOVATION IN THE AI ERA

Li Zeng,Zhimin Chen,Chris K. Y. Lo 글로벌지식마케팅경영학회 2024 Global Fashion Management Conference Vol.2024 No.07

Asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester by undifferentiated cells of white turnip in phosphate buffer/organic solvent

Zhimin Ou,Qingmei Chen,Gensheng Yang,Li Xu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.2

Ethyl (S)-3-hydroxy-3-phenylpropionate was synthesized by asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester with undifferentiated cells of white turnip in phosphate buffer/organic solvent. The conversion increased with the LogP_oct of organic solvent increase. The phosphate buffer (0.2 mol/L, pH 7.0)/dodecane was selected as optimum medium for reduction. The optimal content of dodecane in medium is 10% (v). The conversion decreased with initial substrate concentration increase. Addition of more biomass of plant cells and 10% ethanol as co-substrate can improve conversion. The plant cells can be reused well for three times. The enantiomeric excess of ethyl (S)-3-hydroxy-3-phenylpropionate reached 100% with 1% allyl bromide as inhibitor.

Opposing Effects of ERK and p38 MAP Kinases on HeLa Cell Apoptosis Induced by Dipyrithione

Zhimin Yin,Yumei Fan,Hui Chen,Bo Qiao,Lan Luo,Hsiaoyen Ma,Heng Li,Jihong Jiang,Dezhong Niu 한국분자세포생물학회 2007 Molecules and cells Vol.23 No.1

Cluster-Based Polarized Spectrum Sharing in Channels with Polarization Mode Dispersion

Dongming Li,Zhimin Zeng,Caili Guo,Xiaolin Lin 한국전자통신연구원 2014 ETRI Journal Vol.36 No.3

Polarized spectrum sharing (PSS) exploits the spectrumopportunities in a polarized domain. However, when it comesto wideband environments PSS is impaired by the frequencydependentpolarization mode dispersion (PMD); thus, theeffective throughput of PSS drops. To combat the PMD effect,this work proposes a cluster-based PSS approach to enablePSS on a narrower frequency span. Simulation results showthat the effective throughput of PSS on cluster basisoutperforms that of PSS on bandwidth and subcarrier basis.

Magnetic Resonance Imaging-Related Anatomic and Functional Parameters for the Diagnosis and Prognosis of Chiari Malformation Type I: A Systematic Review and Meta-analysis

Zairan Wang,Zhimin Li,Shiyuan Han,Xianghui Hu,Siyuan Pang,Yongning Li,Jun Gao 대한척추신경외과학회 2024 Neurospine Vol.21 No.2

Objective: Imaging parameters of Chiari malformation type I (CMI) development are not well established. This study aimed to collect evidence of general or specific imaging measurements in patients with CMI, analyze indicators that may assist in determining the severity of CMI, and guide its diagnosis and treatment. Methods: A comprehensive search was conducted across various databases including the Cochrane Library, PubMed, MEDLINE, Scopus, and Embase, covering the period from January 2002 to October 2023, following predefined inclusion criteria. Meta-analyses were performed using RevMan (ver. 5.4). We performed a quantitative summary and systematic analysis of the included studies. This study was registered in the PROSPERO (International Prospective Register of Systematic Reviews) prior to initiation (CRD42023415454). Results: Thirty-three studies met our inclusion criteria. The findings indicated that out of the 14 parameters examined, 6 (clivus length, basal angle, Boogard’s angle, supraocciput lengths, posterior cranial fossa [PCF] height, and volume) exhibited significant differences between the CMI group and the control group. Furthermore, apart from certain anatomical parameters that hold prognostic value for CMI, functional parameters like tonsillar movement, obex displacement, and cerebrospinal fluid dynamics serve as valuable indicators for guiding the clinical management of the disease. Conclusion: We collated and established a set of linear, angular, and area measurements deemed essential for diagnosing CMI. However, more indicators can only be analyzed descriptively for various reasons, particularly in prognostic prediction. We posit that the systematic assessment of patients’ PCF morphology, volume, and other parameters at a 3-dimensional level holds promising clinical application prospects.

Novel splice isoforms of pig myoneurin and their diverse mRNA expression patterns

Guo, Xiaohong,Li, Meng,Gao, Pengfei,Cao, Guoqing,Cheng, Zhimin,Zhang, Wanfeng,Liu, Jianfeng,Liu, Xiaojun,Li, Bugao Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.