Reduction of d-lactate content in sauerkraut using starter cultures of recombinant Leuconostoc mesenteroides expressing the ldhL gene

Jin, Q.,Li, L.,Moon, J.S.,Cho, S.K.,Kim, Y.J.,Lee, S.J.,Han, N.S. Society for Bioscience and Bioengineering, Japan ; 2016 Journal of bioscience and bioengineering Vol.121 No.5

<P>The n-form of lactate, which causes metabolic stress upon excessive dietary intake, is mainly produced by Leuconostoc sp., the predominant species in sauerkraut. To shift the metabolic flux of D-lactate from pyruvate to L-lactate, we expressed the L-lactate dehydrogenase (ldhL) gene in Leuconostoc mesenteroides ATCC 8293. The IdhL gene from Lactobacillus plantarum was introduced into L. mesenteroides using the shuttle vectors pLeuCM and pLeuCM42. To elevate the expression level of IdhL in L. mesenteroides, the nucleotides for pyruvate kinase promoter were fused to IdhL and cloned into above vectors to construct pLC18pkL and pLC42pkL. As results, introduction of pLC42pkL in L. mesenteroides significantly improved both L-LDH activity and L-lactate productivity during fermentation, decreasing the D-/L-lactate ratio. When used as a starter culture for sauerkraut fermentation, recombinant L. mesenteroides harboring pLC42pkL increased L-lactate concentration and decreased D-lactate concentration compared to the wild type strain. We newly developed a recombinant L. mesenteroides which has high L-lactate dehydrogenase activity and applied this strain to minimize the harmful effect of D-lactate during the sauerkraut fermentation. To the best of our knowledge, we demonstrate for the first time the effective use of recombinant Leuconostoc sp. for quality improvement of fermented foods. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.</P>

여대생의 인유두종 바이러스 및 인유두종 바이러스 백신 관련 지식과 예방접종 실태와의 상관관계

Xu, Meiling,김현지,이희수,이윤지,한동연,박소영,엄효윤,최정민,홍서진,홍예림,이경은,이혜림 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51

Purpose: Human Papillomavirus (HPV) vaccination is readily available, but immunization rate is minimal. Lack of knowledge concerning the HPV infection and vaccine is expected as the primary reason. The correlation between knowledge levels and vaccination will provide applicable guidelines for successful interventions. Method: 309 female university students have completed surveys September 2016, reporting of HPV infection knowledge, HPV vaccine knowledge, source of knowledge, and attitudes concerning vaccination. Result: HPV infection and vaccine knowledge levels were both suboptimal(9.57±1.84, 7.59±1.58), and the Internet(16.2%) was recognized as the most generally used means of obtaining information on the HPV vaccine. However, vaccination rates were reported to be low(28.2%), with lack of information(46.6%) hindering immunization. Correlations were found between HPV infection knowledge and cervical cancer insight, but the correlations between knowledge levels and vaccination rates were insignificant. Conclusion: Prior information on cervical cancer had an effect on the knowledge levels of HPV infection, but other factors were insignificant. Lack of information on the HPV vaccine was speculated, thus programs should focus on accessibility and effectiveness.

Interaction of silver(I) and copper(I) with an O₂S₂-macrocycle - A comparative structural study

Kim, S.,Lee, E.,Lee, S.Y.,Lee, S.S.,Lindoy, L.F. Elsevier Sequoia [etc.] 2014 Inorganica chimica acta Vol.417 No.-

The 14-membered O<SUB>2</SUB>S<SUB>2</SUB>-macrocycle L reacts with AgPF<SUB>6</SUB> in acetonitrile/dichloromethane (1:1) to yield the cyclic, double S-Ag-S bridged complex, [Ag<SUB>2</SUB>L<SUB>2</SUB>(CH<SUB>3</SUB>CN)<SUB>4</SUB>](PF<SUB>6</SUB>)<SUB>2</SUB> (2), in which each Ag(I) is bound to two exo-oriented S donors arising from different macrocycles to form a 12-membered, di-Ag metallacycle. Two PF<SUB>6</SUB><SUP>-</SUP> ions form weak intramolecular contacts [Ag...F, 2.925(2)A] between the Ag centres in a bridging bidentate fashion. The coordination sphere of each Ag is completed by two weakly bound acetonitrile molecules. The respective macrocyclic ligands are arranged trans to the mean plane through the metallacycle. When the above synthetic procedure was repeated employing methanol/dichloromethane (1:1) as solvent, a complex of type [Ag<SUB>2</SUB>L<SUB>2</SUB>](PF<SUB>6</SUB>)<SUB>2</SUB> (3) was obtained whose structure incorporates a similar 12-membered metallacycle but with the respective macrocyclic rings now oriented towards the same side of the metallacyclic plane. The reaction of L with AgCF<SUB>3</SUB>SO<SUB>3</SUB> yields [Ag<SUB>2</SUB>L<SUB>2</SUB>(CF<SUB>3</SUB>SO<SUB>3</SUB>)<SUB>2</SUB>].2CH<SUB>3</SUB>CN (4) whose structure resembles that of [Ag<SUB>2</SUB>L<SUB>2</SUB>(CH<SUB>3</SUB>CN)<SUB>4</SUB>](PF<SUB>6</SUB>)<SUB>2</SUB> (2); each complex unit incorporates a di-Ag(I) 12-membered metallacycle, with symmetrical bridging bidentate CF<SUB>3</SUB>SO<SUB>3</SUB><SUP>-</SUP> ions weakly linking Ag centres. Long Ag1...Ag1C contacts are present between pairs of complex units. Reaction of Cu(CH<SUB>3</SUB>CN)<SUB>4</SUB>PF<SUB>6</SUB> (one equiv.) with L in methanol/dichloromethane (1:1) resulted in isolation of [CuL<SUB>2</SUB>]PF<SUB>6</SUB> (5) incorporating a distorted tetrahedral S<SUB>4</SUB>-coordination sphere, with each macrocycle binding as a bidentate ligand via its two (exo-orientated) S atoms. In contrast, reaction of [Cu(CH<SUB>3</SUB>CN)<SUB>4</SUB>]PF<SUB>6</SUB> (two equiv.) with L in either acetonitrile/dichloromethane (1:1) or methanol/dichloromethane (1:1) yielded [Cu<SUB>2</SUB>L<SUB>3</SUB>]PF<SUB>6</SUB> (6) in which the Cu(I) centres are bound exo to the macrocyclic cavity by individual S-donors from one L, with the coordination sphere of each Cu(I) completed by out-of-plane binding of the S<SUB>2</SUB>O donors from a second L. Unsymmetrical F...Cu contacts from a PF<SUB>6</SUB><SUP>-</SUP> anion weakly link the pair of Cu centres in a bridging bidentate fashion.

국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Interaction of copper(II) with N-substituted bis(2-pyridylmethyl)amine derivatives

Kim, M.,Mora, C.,Lee, Y.H.,Clegg, J.K.,Lindoy, L.F.,Min, K.S.,Thuery, P.,Kim, Y. Elsevier 2010 Inorganic chemistry communications Vol.13 No.10

<P>Interaction of copper(II) with the N-substituted bis(2-pyridylmethyl)amine derivatives, (R)-N-1,N-1-bis (pyridine-2-ylmethyl)butane-1,2-diamine (L-1) and (R)-2-(bis(pyridin-2-ylmethyl)amino)butan-1-ol (L-2), has led to isolation of optically active [Cu(L-1)Cl]PF6 (1) and [Cu(L-2)Cl]ClO4 (2), respectively. The X-ray structures of (1) and (2) show that the copper is bound to all four heteroatoms of the respective ligands as well as to a chlorine atom in a distorted square pyramidal arrangement in which the three nitrogens of L-1 or L-2 occupy three positions of each basal plane while the fourth position is occupied by the chloro ligand; apical sites in each case are filled by the amine donor from the NH2-substituted butane arm in L-1 or the (protonated) alcohol oxygen of the 2-aminobutane-1-ol substituent in L-2. To a first approximation the coordination geometry in 2 is distorted square pyramidal; however, the remaining (axial) site on each copper centre is involved in a long contact (2.96 angstrom) with a bound chloro ligand from an adjacent complex which connects individual complex units in a zigzag 1-D polymeric chain, so that the coordination geometry could also be seen as pseudo-octahedral. A temperature-dependent magnetic study revealed the presence of ferromagnetic exchange coupling between copper centres in the chain reflecting the orthogonal structure between the chloro-bridged copper(II) ions; in contrast, and as expected, the discrete complex 1 is magnetically dilute. (C) 2010 Elsevier B.V. All rights reserved.</P>

Mapping the Posterior Ledge and Optic Foramen in Orbital Floor Blowout Fractures

Wong Yu Cong,Goh Doreen S.L.,Yoong Celine S.Y.,Ho Cowan,Cai Elijah Z.,Hing Angela,Lee Hanjing,Nallathamby Vigneswaran,Yap Yan L.,Lim Jane,Gangadhara Sundar,Lim Thiam C. 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.4

Background The posterior ledge (PL) is a vital structure that supports the implant posteriorly during orbital floor reconstruction. This study describes a technique for mapping the PL in relation to the infraorbital margin (IM) in patients with orbital floor blowout fractures. This study establishes the location of the optic foramen in relation to the PL. Methods Facial computed tomography (FCT) scans of 67 consecutive patients with isolated orbital floor blowout fractures were analyzed using Osirix. Planes of reference for orbital fractures, a standardized technique for performing measurements on FCT, was used. Viewed coronally, the orbit was divided into seven equal sagittal slices (L1 laterally to L7 medially) with reference to themidorbital plane. The distances of PL from IM and location of optic foramen were determined. Results The greatest distance to PL is found at L5 (median: 30.1mm, range: 13.5– 37.1mm). The median and ranges for each slice are as follows: L1 (median: 0.0mm, range: 0.0–19.9mm), L2 (median: 0.0mm, range: 0.0–21.5mm), L3 (median: 15.8mm, range: 0.0–31.7mm), L4 (median: 26.1mm, range: 0.0–34.0mm), L5 (median: 30.1mm, range: 13.5–37.1mm), L6 (median: 29.0mm, range: 0.0– 36.3mm), L7 (median: 20.8mm, range: 0.0–39.2mm). The median distance of the optic foramen from IM is 43.7mm (range: 37.0– 49.1) at L7.

A Study of Radioactive Contamination of <tex> $^{\text{40}}\text{Ca}^{\text{100}}\text{MoO}_{\text{4}}$</tex> Crystals for the AMoRE Experiment

Lee, J. Y.,Alenkov, V.,Ali, L.,Beyer, J.,Bibi, R.,Boiko, R. S.,Boonin, K.,Buzanov, O.,Chanthima, N.,Cheoun, M. K.,Chernyak, D. M.,Choi, J.,Choi, S.,Danevich, F. A.,Djamal, M.,Drung, D.,Enss, C.,Fleisc IEEE 2016 IEEE transactions on nuclear science Vol.63 No.2

<P>A calcium molybdate (CaMoO4) crystal scintillator, with molybdenum enriched in Mo-100 and calcium depleted in Ca-48 ((CaMoO4)-Ca-40-Mo-100), was developed by the Advanced Molybdenum based Rare process Experiment (AMoRE) collaboration to search for a neutrinoless double beta (0 nu beta beta) decay of Mo-100. We are planning to use about 10 kg of (CaMoO4)-Ca-40-Mo-100 crystals as cryogenic bolometers for the first phase of the experiment (AMoRE-I) at the Yang Yang underground laboratory (Y2L) in Korea. This experiment calls for an extremely low level of radioactive contamination in detectors, particularly by thorium, uranium, and radium decay chains. We measured scintillation properties and radioactive contamination of CaMoO4 and (CaMoO4)-Ca-40-Mo-100 crystals at the Y2L. We also estimated the acceptable level of internal radioactive background using Monte Carlo simulation for the AMoRE-I.</P>

서울시 지하철 구내의 공기중 분진 농도에 관한 연구

백남원(N.W. Paik),박두용(D.Y. Park),장익선(I.S. Chang),신용철(Y.C. Shin),이정인(J. l. Lee) 한국환경보건학회 1988 한국환경보건학회지 Vol.14 No.2

Airborne dust and asbestos fiber concentrations were determined in subway stations located in Seoul area. Two stations, such as Eulchiro 4.Ka Station of Une #2, constructed during a period of 1980-1984 and Hyehwa Station of Une #4, opened in 1985, were selected. The results of the study are as follows. 1. Daily tirne.weighted average (1W A) concentrations of airborne dusts from 07:00 to 20:00 hours in Line #2 and μne #4 were 0.43:t0.08 mg/m3 and 0.37:t0.12 mg/m3 , respectively. Thus, the dust levels in Líne #2 were significant1y higher than the levels in Line #4( p < 0.05). 2. Dust levels in the morning (07:00-11:00 hours), noon (1 1:00-16:00 hours) and in the evening (1 6:00-20:00 hours) in Líne #2 were 0.47:t0.17 mgjm3 , 0.37:t0.08 mg/m3 , and 0.46土0.07 mg/m3 respectively. Thus, dust levels in the morning and evening (i.e., during rush hours) were significantly higher than levels in the noon ( p < 0.02). However, there was no such difference in dust levels by time in Líne #4. 3. Airborne total dust concentrations were well below the occupational health standard of 10 mg/m3 , however, the levels were exceeding the ambient air standard recommended by the Korean Environment Administration. 4. All of airborne asbestos fiber concentrations were equal to or below 0.005 fibers/cc. The levels are within both occupational health standards and U .S. EPA criterìa. 5. A consideration should be given to the improvements of c1eaning methods (such as use of vacuum c1eaning instead of brushing) and the existing ventilation (such as more aìr change and filtration of supply air) for a reduction of dust levels. 6. It is recommended that routine monitoring of aìrborne dusts and asbestos fibers be conducted because more dusts and asbestos fibers can be produced in the aìr due to the deterioration of facilities by age and water damage in future.

L-Deprenyl exerts cytotoxicity towards acute myeloid leukemia through inhibition of mitochondrial respiration

Ryu, I.,Ryu, M. J.,Han, J.,Kim, S. J.,Lee, M. J.,Ju, X.,Yoo, B. H.,Lee, Y. L.,Jang, Y.,Song, I.-C. Spandidos Publications 2018 Oncology reports Vol.40 No.6

중년층의 비처방약품 오남용 실태 연구

고미현,김민주,김유진,김정호,박예진,유리호,이민영,이송연,조윤진,황선주 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48

Purpose: The purpose of this study was to grasp the status of abusing Over The Counter Drug (OTC Drug) among the middle-aged. Methods: The participants of this study consisted of 175 middle-aged people in Seoul and Gyeoggi-do. Data were collected using a questionnaire, and the study was conducted from September 10 to October 10, 2013. The data were analyzed by t-test, chi-square test, one-way ANOVA and Turkey. Results : As a result, 97 participants(55.4%) out of 175 participants of this study had taken over the counter drug within the past one year. Their average score of abusive behavior of OTC drug was 6.96±3.01 out of 16. There was significant abusive behavior of OTC drug relative to the difference in family monthly income.(F=3.455, p=0.20) and there was significant difference depending on the information source of the OTC Drug.(F=2.56, p=.044) Conclusions : In conclusion, nurses should educate middle-aged people so that they can use over the counter drug more safely and accurately based on these results. In addition, these results will be useful in further studies regarding middle-aged people’s drug abuse according to the preceding data.