http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Gunsup,Cho, SeungChan,Hoang, Phuong Mai,Kim, Dongjun,Lee, Yongjun,Kil, Eui-Joon,Byun, Sung-June,Lee, Taek-Kyun,Kim, Dae-Hyun,Kim, Sunghan,Lee, Sukchan Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.9
3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and $10{\mu}g$ 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 $LD_{50}$ PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% ($5{\mu}g$) and 47% ($10{\mu}g$). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.
Sunghan Kim,Youn Young Lee,Chan Mi Lee,Hee-Jong Koh 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Recent global warming and climate change has presented greater challenge to the global agriculture of having to cope with more severe adversaries from various abiotic stress conditions including drought, cold, and heat. As a preliminary step towards developing a heat-tolerant japonica rice variety through molecular breeding, we examined and compared expression of several genes that have been reported being expressed specifically during rice panicle development in different rice varieties after subjecting them heat stress. Although the induction of these transcripts upon heat treatment was invariably observed in all rice varieties tested, the magnitude and kinetics of the induction were found to be different among these varieties, suggesting possible functional implication of these genes in conferring heat tolerant phenotype during reproductive organ development of these plants. General protein synthesis activity as well as pollen viability incurred by the heat stress treatment were also monitored in these plants and the result showed a close correlation overall with the induction dynamic of these transcripts under heat stress. Therefore, these genes, together with the ones involved in the regulatory network for the expression of them, could serve as candidates for useful markers with which molecular breeding of heat tolerant japonica rice can be facilitated.
Sunghan Kim,Dong-Ryung Lee,Hee-Jong Koh 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
To obtain a better insight into a possible common regulatory mechanism shared in response to heat and cold stresses in model monocot plant, expression profiling for several selected transcripts of rice (O. sativa L.) was examined from meiotic stage panicle samples of a number of different cultivars including both heat/cold tolerant and susceptible varieties. Induction of several of these genes in response to heat stress treatment was observed across all different rice varieties tested, but no differential induction kinetics between the heat-tolerant and susceptible varieties was discernable for these genes. On the other hand, a few genes that are known to be involved in ROS signaling showed different induction kinetics between the tolerant and susceptible varieties, suggesting that these genes might be playing a key role in conferring the heat/cold stress tolerance at reproductive stage of tolerant rice varieties. This approach was also augmented by comparative gene expression data analyses, utilizing expression data of other monocots and dicots model plants available in the database. Based upon these results, a preliminary gene pyramiding effort to estimate contributing factor of several selected alleles after putting these genes together is underway. This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No.PJ009076), Rural Development Administration, Republic of Korea.
Slinky-inspired triboelectric–electromagnetic hybrid generator
Lee Joon-seok,Heo Deokjae,Lyu Han-uk,Hur Ji Woong,Kim Sunghan,Lin Zong-Hong,Park Joong Yull,Lee Sangmin 한국물리학회 2023 Current Applied Physics Vol.49 No.-
Researchers are attempting to develop hybrid generators by integrating several mechanical energy harvesting techniques, although most of them employ complicated device structures and fabrication methods. In this paper, we propose a slinky inspired triboelectric-electromagnetic hybrid generator (S-TEHG) that can easily function as a portable power source for devices used in daily life. This S-TEHG generates a high output using a peak voltage and current output of up to 7.4 V and 83.2 mA, respectively. For practical application as a portable power source in everyday life, the S-TEHG is enclosed (packaged) in an acrylic cylinder. In this study, upon hand waving, the proposed packaged S-TEHG successfully illuminated an array of 15 light-emitting diodes and charged a 47 μF capacitor.
Lee, Ji H.,Park, Sunyoung,Cheon, Soyoung,Lee, Joo H.,Kim, Sunghan,Hur, Dae Y.,Kim, Tae S.,Yoon, Suk R.,Yang, Yoolhee,Bang, Sa I.,Park, Hyunjeong,Lee, Hoon T.,Cho, Daeho WILEY‐VCH Verlag 2011 European journal of immunology Vol.41 No.10
<P><B>Abstract</B></P><P>The active metabolite of vitamin D<SUB>3</SUB>, 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB>, displays anticancer effects by regulating cell cycle and apoptosis in many cancer cells. However, it has not been determined whether 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> increases the susceptibility of cancer cells to NK cells. Here, we investigated the anticancer effect of 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> in human melanoma cell lines by investigating enhancement of NK susceptibility and elucidating the mediator of NK cytotoxicity. 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB>‐resistant melanoma cells (G‐361 and SK‐MEL‐5) treated with 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> showed higher susceptibility to NK cells with up‐regulation of Fas expression. Furthermore, G‐361 cells treated with 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> showed significantly increased caspase activity. In addition to Fas up‐regulation, expression of heat shock protein 60 (Hsp60) was elevated by 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB>. Increased expression of Hsp60 by 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> was related to not only up‐regulation of Fas expression but also to NK susceptibility of G‐361 cells. Taken together, our data suggest that 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> acts as an anticancer agent by increasing expression of Fas on the surface of melanoma cells through Hsp60 induction and strengthens caspase sensitivity to Fas‐mediated apoptotic pathway by NK cells. 1α,25(OH)<SUB>2</SUB>D<SUB>3</SUB> treatment may therefore have a preventive role in melanoma occurrence or potentiate the anticancer effects of NK‐cell immune therapy.</P>