Visible light-driven decomposition of α-pinene and toluene over N and Fe dual-doped one-dimensional titania nanostructures with improved efficiency

Lee, Joon Yeob,Hong, Won-Hwa,Kim, Woong,Park, Sung Hyuk,Jo, Wan-Kuen Pergamon Press 2017 Materials research bulletin Vol.94 No.-

<P><B>Abstract</B></P> <P>This study explores the photocatalytic performance of novel N and Fe dual-doped, one-dimensional TiO<SUB>2</SUB> nanotube structures (N–Fe–TNTs) for the degradation of α-pinene and toluene under visible-light illumination. N–Fe–TNTs were synthesized using a combination of sonochemical-hydrothermal-impregnation processes. N–Fe–TNT hybrids showed higher photocatalytic efficiency compared to pure TNT, N-doped TNT, and Fe-doped TNT. Specifically, the average decomposition efficiencies for α-pinene and toluene over N–Fe–TNT-1.0 were 94.6% and 88.7%, respectively. Moreover, the photocatalytic efficiency of the N–Fe–TNT hybrids improved as the Fe loading was increased gradually from 0.35% to 1.0%, though a further increase in Fe loadings led to reduced photocatalytic efficiency. The mineralization efficiencies of α-pinene and toluene were 43.2% and 47.7%, respectively. Gaseous intermediates were determined during the photocatalytic processes. Overall, the synthesized N–Fe–TNTs could be used efficiently to decompose α-pinene and toluene under visible-light exposure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> N–Fe–TNTs were prepared via a combined sonochemical-hydrothermal-impregnation process. </LI> <LI> N–Fe–TNT hybrids showed improved efficiency compared to pure TNT and N-TNT. </LI> <LI> Mineralization efficiency and intermediates for photocatalysis were determined. </LI> <LI> N–Fe–TNT with a Fe loading of 1.0% showed the maximum photocatalytic efficiency. </LI> <LI> N–Fe–TNTs could be used capably to control pollutants with an efficiency up to 95%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Three Cases of Rare Anatomic Variations of the Long Head of Biceps Brachii

Sang-Ho Kwak,Seung-Jun Lee,Byung Wook Song,Min-Soo Lee,Kuen Tak Suh 대한견주관절의학회 2015 대한견주관절학회지 Vol.18 No.2

In general, the long head of the biceps brachii originates from the superior glenoid labrum and the supraglenoid tubercle, crosses the rotator cuff interval, and extends into the bicipital groove. However, rare anatomic variations of the origins of the long head have been reported in the past. In this report, we review the clinical history, radiologic findings, and arthroscopic identifications of 3 anatomic variants of the biceps tendon long head. As the detection of long head of biceps tendon pathology during preoperative radiologic assessment can be difficult without prior knowledge, surgeons should be aware of such possible anatomic variations.

Pollen Allergic Risk Assessment of Genetically Modified Virus Resistant Pepper and Functional Chinese Cabbage

Lee, Ju-Suk,Kim, Ye-Jin,Ryu, Ki-Hyun,Han, Tae-Ho,Park, Kuen-Woo,Chung, Kyu-Hwan,Lee, Chan,Lee, Gung-Pyo,Kim, Sun-Hyung,Hong, Jin-Sung,Park, Young-Doo,Woo, Eun-Taeck,Park, Sung-Chul,Son, Dae-Yeul 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2

Pollen of genetically modified (GM) pepper containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and GM Chinese cabbage with high phenylethylisothiocyanate (PEITC) content was investigated for assessment of allergic risk. Amino acid (AA) sequences of the inserted gene products of GM virus resistant pepper and GM Chinese cabbage with high PEITC content were compared with those of known allergens. No known allergen greater than 35% AA sequence homology, over 80 AA window or more than 8 consecutive identical AA was found. Protein patterns of GM/non-GM pepper and Chinese cabbage pollen extracts in SDS-PAGE analysis showed the same distribution of protein bands among the GM and non-GM pepper or Chinese cabbage, respectively. Sera from pollen allergic patients showed some IgE reactivity via immunoblotting and ELISA; however, no differences were observed between the pollen of GM and non-GM pepper or Chinese cabbage, respectively. Based on these results, we conclude that pollens of the virus resistant GM pepper and GM Chinese cabbage with high PEITC have no differences in their protein composition or allergenicity relative to non-GM pepper and Chinese cabbage.

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver

Lee, Sang R.,Lee, Seung-yeon,Kim, Sang-yun,Ryu, Si-yun,Park, Bae-kuen,Hong, Eui-Ju Elsevier 2017 JOURNAL OF BIOMEDICAL RESEARCH -ELSEVIER- ENGLISH Vol.31 No.5

<P>Sex steroids, also known as gonadal steroids, are oxidized with hydroxylation by cytochrome P450, glucuronidation by UDP-glucuronosyltransferase, sulfation by sulfotransferase, and<I>O</I>-methylation by catechol <I>O</I>-methyltransferase. Thus, it is important to determine the process by which inflammation influences metabolism of gonadal hormones. Therefore, we investigated the mechanism of metabolic enzymes against high physiologic inflammatory response<I>in vivo</I> to study their biochemical properties in liver diseases. In this study, C57BL/6N mice were induced with hepatic inflammation by diethylnitrosamine (DEN) exposure. We observed upregulation of Cyp19a1, Hsd17b1, Cyp1a1, Sult1e1 in the DEN-treated livers compared to the control-treated livers using real time PCR. Moreover, the increased Cyp19a1 and Hsd17b1 levels support the possibility that estrogen biosynthesis from androgens are accumulated during inflammatory liver diseases. Furthermore, the increased levels of Cyp1a1 and Cyp1b1 in the hydroxylation of estrogen facilitated the conversion of estrogen to 2- or 4-hydroxyestrogen, respectively. In addition, the substantial increase in the Sult1e1 enzyme levels could lead to sulfate conjugation of hydroxyestrogen. The present information supports the concept that inflammatory response can sequester sulfate conjugates from the endogenous steroid hormones and may suppress binding of sex steroid hormones to their receptors in the whole body. </P>

The Selection Criteria of Korean Astronauts

Lee Joo-Hee,Choi Gi-Hyuk,Kim Youn-Kyu,Kim Jong-Woo,Lee Won-Kuen,Hwang Sang-Ho 한국우주과학회 2004 한국우주과학회보 Vol.13 No.2

Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model

Lee, Jangwook,Cha, Min-Ji,Lim, Kwang Suk,Kim, Jang-Kyung,Lee, Sang-Kyung,Kim, Yong-Hee,Hwang, Ki-Chul,Lee, Kuen Yong Informa UK Ltd. 2013 Journal of drug targeting Vol.21 No.9

<P>Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(<SMALL>D</SMALL>,<SMALL>L</SMALL>-lactic-<I>co</I>-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks <I>in vitro</I>. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated <I>in vivo</I>. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.</P>

An Enhanced Network-based Mobility Management Protocol for Fast Mobility Support

( Sung-kuen Lee ),( Kyoung-hee Lee ),( Hyun-woo Lee ),( Seng-phil Hong ),( Jinwoo Park ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.11

In this paper, we propose the enhanced network-based mobility management protocol, called enhanced proxy mobile ipv6 (E-PMIPv6), which can provide mobile nodes (MNs) with a fast and efficient mobility service in PMIPv6 domain. The proposed scheme can provide a fast and efficient mobility service to MNs and also the strength of network scalability and stability to an access network by proposing the dynamic virtual hierarchical network architecture. In addition, the pre-authentication procedure for an MN, based on the information of neighbor mobile access gateway (MAG) list in the enhanced-policy server (E-PS), is proposed to support seamless handover by reducing MN`s handover latency. Through performance evaluations of numerical analyses and simulations, we have confirmed and verified the superiority of the proposed scheme compared to the conventional proxy mobile ipv6 (PMIPv6).

In vivo anti-cancer activity of Korean Angelica gigas and its major pyranocoumarin decursin.

Lee, Hyo Jeong,Lee, Hyo Jung,Lee, Eun Ok,Lee, Jae Ho,Lee, Kuen Sung,Kim, Kwan Hyun,Kim, Sung-Hoon,L?, Junxuan Institute for Advanced Research in Asian Science a 2009 The American journal of Chinese medicine Vol.37 No.1

<P>We have reported that a 10-herbal traditional formula containing Korean Angelica gigas Nakai (AGN) exerts potent anti-cancer efficacy and identified decursin and decursinol angelate (DA) from AGN as novel anti-androgens. Here, we determined whether AGN would exert in vivo anti-cancer activity and whether decursin or DA could account for its efficacy. The AGN ethanol extract was tested against the growth of mouse Lewis lung cancer (LLC) allograft in syngenic mice or human PC-3 and DU145 prostate cancer xenograft in immunodeficient mice. The pharmacokinetics of decursin and DA were determined. The AGN extract significantly inhibited LLC allograft growth (30 mg/kg) and PC-3 and DU145 xenograft growth (100 mg/kg) without affecting the body weight of the host mice. Biomarker analyses revealed decreased cell proliferation (Ki67, PCNA), decreased angiogenesis (VEGF, microvessel density) and increased apoptosis (TUNEL, cPARP) in treated tumors. Decursin and DA injected intraperitoneally were rapidly hydrolyzed to decursinol. Decursinol and decursin at 50 mg/kg inhibited LLC allograft growth to the same extent, comparable to 30 mg AGN/kg. Therefore the AGN extract possessed significant in vivo anti-cancer activity, but decursin and DA only contributed moderately to that activity, most likely through decursinol.</P>

Characterization of the surface immobilized synthetic heparin binding domain derived from human fibroblast growth factor-2 and its effect on osteoblast differentiation

Lee, Jue-Yeon,Choo, Jung-Eun,Choi, Young-Sook,Lee, Kuen-Yong,Min, Do-Sik,Pi, Sung-Hee,Seol, Yang-Jo,Lee, Seung-Jin,Jo, In-Ho,Chung, Chong-Pyoung,Park, Yoon-Jeong Wiley Publishers 2007 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Vol. No.

<P>Fibroblast growth factor (FGF)-2 regulates a variety of cellular functions, such as proliferation and differentiation, by binding to cell surface FGF receptors (FGFRs) in the presence of heparin proteoglycans. FGF-2 is known as a heparin-binding growth factor, but the localization of the heparin binding site has not been fully investigated until now. We used two potential heparin binding domains of FGF-2, the residues 105–111 (F105, YKRSRYT) and 119–135 (F119, KRTGQYKLGSKTGPGQK). Peptides could be stably immobilized onto the surface of tissue culture plates. Using solid phase binding assays, we demonstrated that both peptides had higher binding affinity toward heparin compared with nonbinding control sequence. The biological significance of these sites was tested by cell attachment and osteoblast differentiation studies. Cell attachment to the peptides F105 and F119 increased in a dose-dependent manner. Heparin and heparinase treatments decreased cell adhesion to both F105 and F119. This demonstrates that both F105 and F119 interact with cell-surface heparan sulfate proteoglycans, suggesting that FGF-2 has two heparin binding sites. In addition, osteoblast differentiation, confirmed by ALPase activity and mineralization, was increased by surface immobilized peptide F105 and F119. Taken together, these heparin binding peptides could be applied as biological agents enhancing osteoblast differentiation as well as surface modification tools in the tissue regeneration area, especially for bone regeneration. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</P>

T Cell-Specific siRNA Delivery Using Antibody-Conjugated Chitosan Nanoparticles

Lee, Jangwook,Yun, Kyoung-Soo,Choi, Chang Seon,Shin, Seung-Hwa,Ban, Hong-Seok,Rhim, Taiyoun,Lee, Sang Kyung,Lee, Kuen Yong American Chemical Society 2012 Bioconjugate chemistry Vol.23 No.6

<P>The intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNAi) and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies specifically targeting T cells are lacking. We hypothesized that chitosan nanoparticles modified with T cell-specific antibodies would be useful for delivering siRNA to T cells. CD7-specific single-chain antibody (scFvCD7) was chemically conjugated to chitosan by carbodiimide chemistry, and nanoparticles were prepared by a complex coacervation method in the presence of siRNA. The mean diameter and zeta potential of the scFvCD7-chitosan/siRNA nanoparticles were approximately 320 nm and +17 mV, respectively, and were not significantly influenced by the coupling of antibody to chitosan. The cellular association of antibody-conjugated nanoparticles to CD4+ T cell lines as well as gene silencing efficiency in the cells was significantly improved compared to nonmodified chitosan nanoparticles. This approach to introducing T cell-specific antibody to chitosan nanoparticles may find useful applications for the treatment of various infectious diseases.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bcches/2012/bcches.2012.23.issue-6/bc2006219/production/images/medium/bc-2011-006219_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bc2006219'>ACS Electronic Supporting Info</A></P>