새로운 연노출의 효과지표로서의 혈장 δ-aminolevulinic acid 측정의 유용성

이금원,황보영,김용배,리갑수,이성수,장봉기,함정오,안규동,이병국 순천향대학교 산업의학연구소 2001 순천향산업의학 Vol.7 No.1

To evaluate the usefulness of the measurement of plasma δ - aminolevulinic acid (ALAP) as a new effect indicator of lead exposure, the association of ALAP with blood lead, DMSA chelatable lead and bone lead with a special emphasis of genetic interaction of ALAD polymorphism was studied with 419 lead exposed workers and 85 non-lead exposed workers. Lead workers were recruited from storage battery industries, secondary smelting industries and other lead using industries and non-lead workers selected from electronic refrigerator manufacturing industry. Blood lead, DMSA chelatable lead and bone lead were chosen for parameters of lead exposure. Plasma and urinary ALA were selected for the effect parameter of lead intoxication. Information for age, smoking, drinking habit and body mass index were also obtained. Job duration for lead workers was also collected. The overall prevalence of the variant allele, ALAD type 1-2 or 2-2(ALAD2) in lead exposed workers was 10.0% (42 out of 419 lead workers) which was not differed from control workers (9.4% : 8 out of 85). The means blood lead and DMSA chelatable lead of lead workers with ALAD 1(ALAD type 1-1) were lower than those of lead workers with ALAD2, but the differences were not statistically significant. On the other hand, the mean of tibia lead of lead workers with ALADI was higher than that of lead workers with ALAD2, but the difference was not statistically significant. DMSA chelatable lead showed highest correlation with log transformed plasma ALA(LALAP)(r=0.682) and blood lead and tibia lead had r=0.650, r=0,402 correlation coefficient with LALAP respectively On the other hand. log transformed urinary ALA(LALAU) had lower correlation coefficient with all three parameters of lead exposure than LALAP. The equation of robustic simple linear regression of blood lead on LALAP was LALAP=2.0439+0.0165PbB in ALADI lead workers and LALAP=2.031+0.0121PbB in ALAD2 lead workers. ALAD genotype in the multiple regression analysis of blood lead with LALAP after adjusting for possible confounders(sex, ago, BMI, smoking and drinking status and job duration) showed significant main effect on LALAP resulting lower LALAP in ALAD2 lead workers than ALADI lead workers. On the other hand, effect modification of ALAD genotype was observed in the multiple regression analysis of tibia lead with LALAP after adjusting for possible confounders, but there was no significant effect of ALAD genotype in the multiple regression analysis of DMSA chelatable lead with LALAP after covariate adjustment. With above results, plasma ALA was found to be a useful effect parameter of lead exposure compared with urinary ALA. It was also observed that ALAD polymorphism made significant effect on the association of blood and tibia lead with ALAP. Thc lower plasma ALA in ALAD2 lead workers suggested supportive effect of ALAD2 in lead exposure.

여성 연폭로 근로자들의 연폭로 지표와 자각증상에 관한 연구

이금원,임정규,안규동,이병국 순천향대학교 1994 논문집 Vol.17 No.2

The effect of lead on subjective symptoms of lead exposure was studied with 396 women lead workers in 6 storage battery factories as an exposed group to lead and 166 women workers in general manufacturing industries as a non-exposed group to lead. Zinc protoporphyrin in whole blood(ZPP) was selected as an indicator of lead exposure. SGOT and SGPT were also checked to observe the lead effect on liver function of lead workers. Questionnaires of 15 lead related symptoms were provided to all women workers and filled up by themselves and reconfirmed the symptoms answers by interviewing medical doctor. There was significant mean difference of blood ZPP between exposed group (90.0 ± 86.2 ug/dl) and non-exposed group (33.8 ± 29.9 ug/dl), but there was no differences of means of SGOT and SGPT between exposed and non-exposed group to lead. After age stratification, the symptom prevalences of exposed group were higher than non-exposed group in most cases. This trend was strong in the comparison of symptom prevalence of women worekrs whose ages were under 30 years. The symptom prevalence of women workers who worked in medium sized factories was higher than that of women workers working in small sized factories. While the symptom prevalence of all workers was not dose-dependent to ZPP increase in most symptoms category, there was weak trend of dose-respnse relationship of symptom prevalence with ZPP increase in women workers working in medium sized factories, but not statistically significant.

H 추정기를 이용한 시스템 식별

李金源 관동대학교 1997 關大論文集 Vol.25 No.2

For system identification, existing LMS type estimators are widely used. For example Kalman filter is one of them. But since 1980s H cencepts are studied theoretically and recently H estimators are tried by several reserchers. In this paper, a state estimator is derived for the H∞ norm of the estimation error spectrum matrix to be minimized. For the solution of this problem, duality between this problem and the well-known H control problem is derived. So the standard H control problem solution method can be used for the estimation problem solution. The computer simulation results show that H estimator has less estimation error and so this is better than the existing Kalman filter estimator.

잡음제거를 위한 자기동조 제어기 설계

李金源,崔南洙 관동대학교 1996 關大論文集 Vol.24 No.1

In this paper, self-tuning controller is designed, so that Hoo norm of the difference between the desired transfer function and the actual closed loop transfer function is minimized. For solving this problem. Nevanlinna-Pick interpolation method is introduced and 1-step ahead predictor is also utilized for the realizable controller design. A 2nd order system is used for simulation. The effect of the noise to the system output is shown to the rejected in simulation.

지형변화에 따른 2족 로봇의 보행 설계 및 구현

이금원 關東大學校 産業技術開發硏究所 2008 산업기술논문집 Vol.- No.25

The stabilized ambulation design of a biped robot which have 18 degrees of freedom is researched By using inverse kinematics, the angles of motors of each joint are calculated as D-H transformation parameters It is shown that the center of gravity of the robot is stabilized with the added upper body and the robot can accomplish stabilized ambulation in the experiments which is done by using the derived numerical formulas and algorithms.

女高生의 營養知識과 食習慣 및 健康狀態에 관한 硏究

오금향,정낙원,이효지 漢陽大學校 韓國生活科學硏究所 1994 韓國 生活 科學 硏究 Vol.- No.12

The diet has held weight in the basic life of the mankind. Its contents, closely connected with the state of health, play an important role in the physical and mental happiness in life. Especially, the youth at the age of 13 to 19, who show the rapid growth physically, mentally, and psychologically, must acquire nutrition knowledge and form the right diet habits. This research shows how these knowlege and diet habit have an effect on student's health. The following results, gained by means of SPSS/pc+, were analyzed in terms of frequency, percentage, dispersion analysis, X2- test, and pearson's correlation. The results are as follows : 1.The group was made up of the humanity group of 37.2% and the natural group of 34.3% and the vocational group of 28.5%, 48.1% of the subjects for research was at the age of 16. The distribution of their scholastic marks were 26.9% in the upper level 30.2% in the middle level. and 191.% the lower level. 2.In the parents' school carrer of the object students, persons who graduated high school were numerous. The mothers' educational level was than that of the fathers. In the parents' job, fathers engaged in office were 24.0% most of the students' mothers were home makers (53.6%), and mothers engaged in official work were 13.7%. 3.The students took up 61% who answered that knew about nutrition to some degree. Most students responded that they got knowledge and information about nutrition(83.2%). The source which students acquired nutrition knowledge from were schools (48.7%) and mass communication(30%). 4.The frequency of meals in a day, was usually three times. The meal that students were likely to have was breakfast, lunch, and dinner in its order. In most cases, The students skip meals three or four times a week. The meal that students enjoyed was lunch, which was taken regularly where as they had breakfast and dinner irregularly. The reason was that they had no time to eat. After dinner, the students sometimes ate refreshments(64.3%) and it took them tenor twenty minutes to have it. They tended to talk with each other while eating. The students thought that the trouble they're having was manintaining the regular diet habit. 5.The students were in good health, and 74.3% of them had never suffered from any illness, In terms of the physical fidelity its distribution was composed of 「da」 of 48.0% and 「ra」 of. It means that the students are in a normal condition of growth. and 22.4% of the took a variety of medicines for improving their health sight and for treating anemia. 6.There was a meaningful difference between nutrition knowledge of the students and their physical health(P<0.05). The students who knew much about nutrition were physically healthy, and mental health was closely connected with physical health(P<0.01). The students who had a good knowledge of nutrition and a good physical health got good school records.

일부 시판세치제의 비교치아마모도 및 경과시간에 따른 유효불소농도변화에 관한 연구

장덕수,이병진,배광학,방금석,이원재,노정,백대일,김종배 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.3

The purpose of this study was to assess relative tooth abrasivity and to measure effective fluoride concentration of dentifrices selling in Korea for help consumer's selection of suitable dentifrice. Assessment of the abrasion on tooth produced by different dentifrices were carried out on bovine teeth in the laboratory and tested by radioactive dentin abrasion method. And total soluble fluoride concentrations within the dentifrices was determined using fluoride ion selective electrode with standard curve obtained from standard solutions of known fluoride concentration. 10 dentifrice samples were stored at 49t for 15 weeks and the measurements were made for each dentifrice in 1 week, 2 weeks, 3 weeks, 13 weeks and 15 weeks and 11 dentifrice samples were stored at 40.6C for 3 weeks and the measurements were made for each dentifrice in 1 week and 3 weeks. Tooth abrasivity of selling dentifrices is suitable for toothbrushing but dentifrice which recommended for patient who have tooth abrasion or erosion was somewhat high abrasivity level. Effective fluoride concentrations of selling dentifrices were maintained generally optimal level. But some dentifrices contaning CaCO_(3), as abrasive were decreased as time elapsed. It was suggested that relative tooth abrasivity must be marked on package and fluoride level should be maintained acceptable level in dentifrices for consumer's selection of suitable dentifrice.

위장질환에 따른 H.pylori 감염빈도와 검사방법의 비교분석

김대현,조재현,금민수,최성곤,이창형,탁원형,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : H. pylori가 위장관 질환의 병인에 깊은 관련이 있어 이의 적절한 진단 및 치료가 중요하게 대두되고 있다. 이에 저자들은 소화기 증상을 주소로 내원한 환자들을 대상으로 내시경 검사를 실시하여 H. pylori검출을 위한 CLO검사, IgG 항체검사 및 H&E 염색 등을 시행하여 각 질환별로 H. pylori 감염의 양성율을 알아보고 검사법에 따른 민감도 및 특이도를 그 유용성에 대하여 조사하였다. 대상 및 방법 : 1996 4월에서 8월까지 경북대학교병원에 소화기증상을 주소로 내원한 총 313명의 환자를 대상으로 내시경 검사를 실시하여 H. pylori 검출을 위한 CLO 검사, IgG 항체검사, 조직염색 검사를 실시하였다. 결과 : 전체 대상 환자수는 313명이었고 연령별 분포는 16세부터 80세 까지 다양하였으며 그중40대, 50대, 60대가 각각 69예(22%), 73예(23.3%), 65예(20.8%)로 전체 환자중 66%를 차지하였다. 남녀비는 2.07 대 1로 남자가 많았다. 대상환자의 평균나이는 52±15세였고, 전체 대상환자에서 H. pylori 양성군과 음성군의 평균나이는 각각 50±15세, 54±15세 였고, 궤양환자군에서 H. pylori 양성군과 음성군사이의 평균나이는 각각 49±14.8세, 55±13.8세로 나타났다. 각 질환별분포는 위염이 72명(23%), 십이지장 궤양이 85명(27.2%), 위궤양이 80명(25.6%), 위암이 20명(6.4%), 비궤양성 소화불량이 13명(4.2%), 기타가 43명(13.7%)이었으며, H. pylori 감염 양성율은 위염이 71%, 십이지장 궤양이 86.8%, 위궤양이 58.3%, 위암이 75%, 비궤양성 소화불량이 75%였으며 특히 유문륜 궤양에서는 100%의 양성율을 보였다. 소화성 궤양군과 비궤양군에서의 H. pylori 양성율을 비교해 본 결과 궤양군에서는 73.1%, 비궤양군에서는 72%로 통계학적으로 차이는 없었다(P=0.91). 소화성 궤양환자에서 출혈이 동반된 군과 동반되지 않은 군에서의 H. pylori 양성율을 비교해 본 결과 출혈이 동반된 군에서의 양성율은 54.5%였고, 출혈이 동반되지 않은 군에서는 77.5%의 양성율을 보여 출혈이동반되지 않은 군에서 통계학적으로 유의하게 높았다(P=0.014). 나이에 따른 양성율을 알아본 결과 50세 이하에서는 78.5%였고, 50세 이상에서는 67.5%로서 양성율이 낮았으나 통계학적으로 유의성은 없었다(P=0.052). 각 검사법에 따른 민감도 및 특이도는 전체 환자중에서는 CLO검사가 88.9%, 96.1%, IgG항체검사 90.8%, 58.5%, H&E 염색검사 86.9%, 79.2%로서 민감도는 비슷하게 나왔으나 CLO검사는 상대적으로 특이도가 다른 검사법에 비해 높았다. 궤양군에서의 CLO검사의 민감도와 특이도는 92.0%, 95.6%였고, 비궤양군에서의 민감도와 특이도는 84.3%, 96.8%로서 비궤양군에서는 CLO검사의 민감도는 약간 낮았다. 나이에 따른 CLO 검사의 민감도 및 특이도는 50세 이하에서는 91.5%, 93.1% 였고, 50세 이상에서는 86.3%, 97.9%로서 50세 이상에서는 상대적으로 CLO검사의 민감도가 낮았다. 결론 : 본 연구에서는 위장질환에 따른 H. pylori 양성율을 알아보았는데, 다른 보고에서 처럼 높게 나왔으며, 진단 방법으로 사용한 CLO검사, IgG항체 검사, 조직염색검사 등은 모두 비교적 높은 예민도를 나타냈고, 그중 CLO검사가 간편하고 경제적이라는 면에서 임상적으로 유용한 검사로 여겨지나 상대적으로 CLO검사의 민감도가 낮은 비궤양성 질환과 50세 이상의 환자군에서는 CLO검사 외에 다른 검사방법을 병행함으로써 H. pylori의 진단율을 높일 수 있을 것으로 사료된다. Background: Gastric colonization by Helicobacter. pylori is common among patients with peptic ulcer and gastritis. And various diagnostic tests are available in confirming H. pylori infection. The aim of this paper is to estimate the prevalence of H. pylori infection in Gastroduodenal disorders and compare the sensitivity and specificity of the most widely available tests. Methods: A total of 313 patients were tested for H. pylori infection by IgG Ab, CLO test and H&E stains. Results: The prevalence of H. pylori infection was significantly higher in duodenal ulcer(86.8%) than in gastric cancer(75%), gastritis(71%) and gastric ulcer(58.3%)(P=0.009). The sensitivity and specificity of each test was as follows: CLO test(88.9%, 96.1%), IgG Ab test(90.8%, 58.5%), H&E stain(86.9%, 79.2%). Sensitivity of CLO test in peptic group was 92.0%, whereas in non-peptic group, it was 86.3%. Sensitivity of CLO test in age under 50 was 91.5%, that over 50 it was 86.3%. Conculsions: There is a strong association between H. pylori and gastrointestinal disorders. CLO tests have relatively high sensitivity and specificity, especially in peptic group and younger age(below 50 yr)group. But in those patients in which CLO tests are less sensitive (age>50 or non-peptic group), more than one test may be necessary to diagnose the presence of the organism.

The Role of Thymic Stromal Lymphopoietin (TSLP) in Glomerulonephritis

Lee, Keum Hwa,Yang, Jae Won,Cho, Jin Young,Lee, Joo Yup,Lim, Eun Kyung,Eisenhut, Michael,Jeong, Dong Yeon,Steingroever, Johanna,Shin, Jae Il Korean Society of Pediatric Nephrology 2018 Childhood kidney diseases Vol.22 No.1

Thymic stromal lymphopoietin (TSLP) is an interleukin-7-like cytokine that is an important trigger and initiator of many allergic diseases. TSLP promotes a T-helper type 2 (Th2) cytokine response that can be pathological. A relationship is formed both at the induction phase of the Th2 response through polarization of dendritic cells to drive Th2 cell differentiation and at the effector phase of the response, by promoting the expansion of activated T cells and their secretion of Th2 cytokines and TSLP. In transgenic mice with TSLP overexpression, it has been reported that TSLP leads to the development of mixed cryoglobulinemic membranoproliferative glomerulonephritis. In addition, TSLP can play an important role in the pathogenesis of IgA nephropathy and systemic lupus erythematosus-related nephritis. From our knowledge of the role of TSLP in the kidney, further studies including the discovery of new therapies need to be considered based on the relationship between TSLP and glomerulonephritis.

Evaluation of<i>phoP</i>and<i>rpoS</i>mutants of<i>Salmonella enterica</i>serovar Typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice

Lee, Hui-Young,Cho, Sun-A,Lee, In-Soo,Park, Jong-Hwan,Seok, Seung-Hyeok,Baek, Min-Won,Kim, Dong-Jae,Lee, Seok-Ho,Hur, Sook-Jin,Ban, Sang-Ja,Lee, Yoo-Kyoung,Han, Yang-Keum,Cho, Young-Keun,Park, Jae-Hak Oxford University Press 2007 FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY Vol.51 No.2

<P>The attenuation and immunoenhancing effects of rpoS and phoPSalmonella enterica serovar strain Typhi (Salmonella typhi) mutants have not been compared. Here, three S. typhi deletion mutants (phoP, rpoS, and rpoS-phoP double mutant) are constructed and these mutants are characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type Ty2. It was found that phoP and phoP-rpoS deletion mutants are less invasive to HT-29 cells than the wild-type Ty2 and the rpoS single-deleted strain. The LD(50) of immunized mice was higher for phoP than for rpoS mutants, and the highest for the phoP-rpoS double mutant. In addition, all S. typhi mutants showed an increase in the specific serum IgG levels and T-cell-mediated immunity, and showed equal protection abilities against a wild-type Ty2 challenge after two rounds of immunization in BALB/c mice. It is concluded that phoP genes appear to play a more important role than rpoS genes in both cellular invasion and virulence of S. typhi, but not in immunogenicity in mice. Furthermore, the data indicate that the phoP-rpoS double mutant may show promise as a candidate for an attenuated typhoid vaccine.</P>