횡성댐 水沒地域內의 植生에 關한 硏究 : 移植對象區域을 中心으로 Centered with Subdivided Area to Select Plants Capable of Transfer

이우철,이기의,서옥하,조현길,백원기,김경진 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

본 조사 연구는 江原道 橫成郡 甲川面과 橫成邑 일부를 포함하는 지역에 횡성댐이 건설됨에 따라 수몰되어질 自然生態系의 復元과 保存計劃의 일부로 수행되어졌다. 移植對象區域인 7개소를 중심으로 綠地自然度, 植生現存量 및 생산량 種組成과 植生에 관해 조사하였으며 그 결과는 다음과 같다. 1. 綠地自然度를 등급별로 사정한 결과 등급 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%)의 8개 등급으로 구분되었다. 2. 전체조사지역의 現存量과 生産量은 각각 88,436.3 ton/year 과 12,960.3 ton/year 이었다. 3. 조사지역의 陸上植物의 種組成은 86科, 221屬, 306種, 1變種, 3品種의 총 310種으로 조사되었다. 4. 선정된 7개의 植生調査地點에서는 소나무군락이 憂占하며, 그밖에 신갈나무군락, 떡갈나무군락, 상수리나무군락으로 나타났다. This study was carried out in a part of conservative plans recovering natural ecosystem which will be submerged, as Hoengseong dam is being constructed around Hoengseong-Eup and Gabcheon-Myun. The submerged area was divided into 7 small areas to figure out what kinds of species of plants are and will be according to detailed geographic characteristics. In the center of the subdivided areas. Degree of Green Naturality, standing biomass, net 36production per year, and composition of plant species were investigated. The results are as following; 1. The surveyed area was classified into 8 degree by Degree of Green Naturality, and the appeared degree and the rate of occupation were 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%). 2. Standing biomass was 88,436.3 ton/year and net production per year was 12,960.3 ton/year. 3. The list of vascular plants identified in this study consisted of 86 families, 221genera, 1 variety, 3 formae and 306 species, totally summiting upto 310 species. 4. The 7 surveyed areas were largely occupied by Pinus community. In addition, there were Quercus mongolica, Quercus dentata, and Quercus acutissima communities.

日本의 敎員養成制度 改革

李相魯,姜信珠,安泰潤,奇宇恒 慶北大學校 師範大學 1985 敎育硏究誌 Vol.27 No.-

It is said that Korean teacher education is facing some critical Problems. We think that we can get some imformations from the reexaminations on the recent reforms in Japaness teacher education system. 1) As the basic orientation of teacher education, we need to distinguish open system and closed system; teacher education in all college level and teachers college level. Institutional improvements should be directed to democratization and specialization; abuses in massproductive teacher education are required to correct. 2) In principle, teacher education should be enforced solely in regular college level; through strengthening teaching method course, teaching subject courses, and student teaching practices, professionally of teacher education must be elivated. 3) As teacher education heavily depends upon teacher employment directly or indirectly, the plan of the teacher demand and supply is needed to be reasonable. And incentive systems for teachers are demanded. 4) In-service education should be qualified to enhance the autonomy of teaching profession. 5) The Co-research between colleges, educatibnal institutes, and schools is expected to strengthen and enlarge action research.

자외선 A-1으로 치료한 사마귀양 균상 식육종 1예

서기석,이진우,김상태 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

Verrucous mycosis fungoides is very rare variant of cutaneous T cell lymphoma that manifests verrucous crusted lesion and may be clinically misdiagnosed as a wart, deep fungal infection or atypical mycobacterial infection. If it is located acrally, it might then be better classified as mycosis fungoides palmaris et plantaris or pagetoid reticulosis. We report a case of solitary verrucous mycosis fungoides on the left knee in 25-year-old woman successfully treated ultraviolet A-1 phototherapy.

Pulsed Nd:YAG 레이저 조사와 불소화합물 도포가 치아 내산성에 미치는 영향

이안희,기우천 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

This study was designed to determine the most effective concentration of fluoride and levels of laser irradiation for the remineralization of decayed teeth. After irradiation with a pulsed Nd : YAG laser and the topical application of fluoride, phosphate and fluoride concentration in enamel were measured. And then the changes on surface enamel using an scanning electron microscope were observed. Samples by extracting healthy, permanent, mandibular bovine teeth with no caries were obtained. Among them 371 healthy samples were selected and artificial carious lesions were made. 20 samples were assigned to each experimental group. After irradiations with a pulsed Nd : YAG laser with total energy densities of 10J/㎠, 20J/㎠ and 30J/㎠ for each group. On the teeth, 2% NaF, 1.9% NH_4F, 1.6% TiF_4. Elmex gel(amine fluoride) and 1.23% APF gel were applied. After pH circulatory procedures, concentrations of fluoride with an Ionalyzer (Orion Research. Model 901, U.S.A.) and phosphates with a UV/Vis spectrophotometer (Uvikon 860, Kontron Co., Switzerland) were measured. By etching the teech in layers and measuring fluoride concentrations, a profile of fluoride penetration according to depth could be developed. And also the changes on the surface of the enamel using an electron scanning microscope were observed. The comparative analysis yielded the following results; 1. Phosphate concentration was low in all groups compared with the control group except for teeth treated Elmex gel, irradiated with 10J/㎠ and 30J/㎠ energy densities. Teeth treated with APF gel and 30J/㎠ irradiation had the lowest phosphate concentration. 2. Among all groups, fluoride concentrations in tooth enamel were highest in APF gel and NaF groups irradiated at 30J/㎠. The APF gel group had the highest fluoride concentrations across all energy densities. 3. In the APF gel group, and the NaF group, the greater the energy density of the laser, the higher the fluoride concentration in the enamel. 4. In all groups, the concentration of fluoride in the enamel by depth tended to decrease with depth. 5. Under the scanning electron microscope, under the condition of irradiation with 20J/㎠, enamel crack was detected for the first time. In the NH_4F group, spherical deposits were found on the surface of the enamel, and in the TiF_4 group the surface of enamel was covered with an irregular, thin membranous mass in places. In the APF gel and NaF groups irradiated with 10J/㎠, spherical and irregular particles covered the teeth. When these groups were irradiated at 20J/㎠, they were covered with amorphous crystals. These results suggest that one could obtain more effective anticariogenic effects without damage to teeth when less than 20J/㎠ energy densities and APF gel are used.

β-Carotene 이 혈중 효소활성도에 미치는 영향

이상한,우기민,조만희,장예진,김창세,김현철 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

This study was carried out in order to observe the effects of ethanol and β-carotene on the cholesterol, Triglyceride, LDL-cholesterol, HDL-cholesterol, glutathione peroxidase and catalase activities. The normal white S.D rats were classified into 4 group: 0.9% NaCl administration and 20% ethanol administration were referred to as group A, 20% ethanol and 50% β-carotene mixture administration was group B and 50% β-carotene administration was group C for the 30 day-feeding experiment. The results were obtained as following: 1. The blood lipid concentration was most highly increased in group A, was almost normal in group B, but significantly decreased in goup C. 2. The blood antioxidant enzyme activities were significantly decreased in group A, but significantly increased in goup B and C. 3. The blood β-carotene concentration were significantly decreased in group A, and slightly decreased in group B. Surprisingly it was increased in group C.

방사선조사에 따른 케이블용 절연재료의 유전특성

이우선,박정기,이영식 조선대학교 에너지.자원신기술연구소 2001 에너지·자원신기술연구소 논문지 Vol.23 No.2

This study describes the diectric effect of γ-ray irradiation on the properties of insulation materials for low voltage cables in a nuclear power plant. The radiation effects were characterized by measuring OIT, electrical properties of the irradiated specimens. As a result, they showed the decrease of OIT and the increase of chemical structural defects with the increase of γ-ray amount. Also, the electrical properties such as dielectric constant, tan δ and current were changed by aging. These changes may come from the increase of chemical structural defects by γ-ray irradiation.

Lumbricus rubellus의 내열성 지질분해효소에 관한 연구

우기민,이상한,조만희 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

여러 가지 chromatography법들(anion exchange, hydrophobic interaction, gel filtration)을 이용하여 Lumbricus rubellus로부터 지질분해효소를 정제하였다. 정제된 효소는 SDS-PAGE와 Superose-12 gel filtration을 통하여 32 kDa의 단일체임을 알 수 있었다. 본 연구에서 정제된 Lumbricus rubellus의 지질분해 효소를 Lumpase Ⅰ (Lumbricus + lipase Ⅰ)이라고 명명하였으며, 이 효소는 FDA를 기질로 이용하였을 때 10μM의 높은 친화도를 보였다. 또한 상업적으로 많이 이용되고 상품화된 다른 지질분해효소와 그 특이적 활성을 조사하였을 때, 최소한 200배 이상 높은 단위활성을 가진 것으로 판명되었다. 또한 다양한 기질 특이성을 가져 여러 가지 지질 기질들을 분해하였다. 높은 온도(60℃)에서 Lumpase Ⅰ의 활성을 측정하였을 때, 다른 내열성 지질분해효소처럼 한 시간 이상 그 활성이 잘 나타내어 열에 강한 지질분해효소로 판명되었다. 여러 가지 화학 반응물들의 영향을 조사해 본 결과, Lumpase Ⅰ은 serine을 활성 부위(active site)로 가지고 SDS같은 detergent에 강한 효소로 나타났다. 따라서, 본 연구에서 정제된 Lumpase Ⅰ은 지질분해효소를 이용한 의약산업, 환경산업, 식량산업, 세제 등 다양한 산업분야에 이용가치가 매우 높은 효소로 여겨지며, 지속적인 연구가 진행 중이다.

단백질 및 1가 양이온을 통한 proteasome의 활성조절에 관한 연구

우기민,이상한,조만희 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Proteasome은 일반적으로 세포내 환경과 매우 상이한 조건하에서 연구된다 (실험용 완충액에는 1기가 이온들이나 여러 단백질들이 결여되어 있다). 특이하게도 본 연구결과 낮은 농도의 Na^(+)와 K^(+)는 20S proteasome의 단백질 분해 활성을 현저히 저하시키지만 26S proteasome의 단백질 분해 활성에는 크게 영향을 미치지 못하였다. Na^(+)와 K^(+)는 20S proteasome의 chymotrypsin 유사 분해활성을 1-2 mM의 Ki 값으로 매우 민감하게 억제하였다. 이에 비해 기타 다른 1가 양이온 (Li^(+), Rb^(+), 또는 Cs^(+))은 미미한 억제효과를 나타내고, 2가 양이온 (Mg^(2+))은 20S proteasome의 chymotrypsin 유사 분해활성을 오히려 활성화시켰다. 이러한 이온들은 trypsin 유사 활성과 post-acidic 활성에도 유사한 억제효과를 보였다. 또한, Na^(+)와 K^(+)는 20S proteasome에 의한 casein, oxidized RNase A, a-lactalbumin 같은 단백질들의 분해를 강력하게 억제시키지만, 26S proteasome에 의한 ATP-의존적 단백질 분해 활성에는 영향을 미치지 못한다. 일반적으로 세포내 단백질농도는 매우 높은 것으로 알려져 있는 바, proteasome의 분해활성에 미치는 단백질의 영향을 조사한 결과, 무작위의 다양한 단백질들(0.1㎎/㎖)도 1가 양이온(Na^(+)와 K^(+))의 경우와 같이 20S proteasome의 펩타이드 분해활성을 억제하였지만 26S proteasome의 활성에는 아무런 영향을 미치지 않았다. 이러한 억제작용은 경쟁적이지 않다. BSA나 ovalbumin 같은 비가수분해 단백질들이 20S proteasome의 펩타이드 분해활성을 강력히 억제한였으므로 이러한 억제현상이 비경쟁적이라는 것을 알 수 있다. 비록 Na^(+), K^(+)와 단백질들이 20S proteasome을 비활성화 형태로 유지시키지만, 0.02% SDS에 의한 20S proteasome의 활성증가를 막거나 억제시키지는 못하였다. 이 결과로 고농도의 이온과 단백질이 존재하는 세포내 조건에서는, 독립적인 20S proteasome은 잠재적으로 비활성화된 형태로 존재하며, 19S ATPase 복합체나 11S 같은 proteasome 활성복합체가 결합한 후에 비로소 26S proteasome의 활성화된 형태로 전환되는 기작을 갖는 것으로 여겨진다. 또한, Na^(+)나 K^(+), BSA나 ovalbuim의 첨가는 비활성화 형태의 20S proteasome을 이용한 많은 연구를 보다 용이하게 할 것으로 기대된다.

데이타베이스 릴레이션의 튜랜잭션에 대한 할당문제 분석

이우기 聖潔大學校 自然科學硏究所 1996 自然科學硏究 Vol.1 No.-

In this paper a scheme is suggested that deals with database queries and relevant relations. Queries that struggle among other queries to occupy a portion of databases exclusively can be decomposed into several transactions as an atomic processing unit. Here a scheduling algorithm with greedy beuristics is addressed to solve the allocation problem among competing transactions, and the computational complexity of the algorithm is analyzed within those problem domain.

Thermoplasma acidophilum의 20S Proteasome에 의한 ATPase-활성적 단백질분해에 관한 연구

우기민,장예진,조만희,김창세,김완종,조성호,이상한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The eukaryotic 26S proteasome is an ATP/ubiquitin-dependent proteolytic complex consisting of the 20S core particle and 19S ATPase complex. However, because of its complexity and unstable properties, this study was carried out to present more simple and stable model for the ATP-activated proteolytic complex in prokaryotes which can take the place of the eukaryotic 26S proteasome. For this purpose, recombinant Thermoplasma 20S proteasome (T20S) and Methanococcus MS4, a sequence homolog of one ATPase subunit in the 19S ATPase complex, were successfully isolated from Escherichia coli (E. coli). The α and β subunits of T20S expressed in E. coli could assemble for themselves, and showed the peptide-hydrolyzing activity. Whereas both T20S and R20S (the 20S complex from rabbit skeletal muscle) had the highest peptidase activity against Suc-LLVY-AMC, a good substrate for chymotrypsin-like peptidase activity, the specific activity of T20S was slightly lower than that of R20S. In addition, several reagents such as KCI, SDS, and ovalbumin were shown to have different effects on the peptidase activities between T20S and R20S. When the ATPase activity of the purified MS4 were assayed , the Km for ATP was about 0.5 mM, and casein could stimulate the activity more than 2 fold without the change in Km. This result implicates that protein-activated ATPase may induce the conformational change of casein, and therefore suggests that MS4 ATPase may activate the proteolytic activity of the 20S proteasome via accelerating the recognition and translocation of the protein substrates.