수부 주상골 요부의 전위골절에 대한 수술적 치료

조용만,이영기,강흥식,리건영,이천동,오상수 대한골절학회 1997 대한골절학회지 Vol.10 No.1

The authors surveyed the clinical study of 17cases of scaphoid fractures from March 1989 to September 1994 with minimal I year follow up at Department of Orthopedic Surgery, Lee-Rha general Hospital. The following conclusions were obtained through clinical survey. l. Of all l 7 cases, they were l3 males and 4 females; Among the 13 males, 10 cases were aged between 20 and 39 years. forming 56.8% of all. 2. All 17 cases were treated by open reduction. Unions were obtained at average l l.5 weeks in 7 cases treated with Herbert screw fixation and l 2 weeks in l 0 cases treated with K-wire fixation. 3. Two of ten K-wire treated cases resulted in delayed union, one of seven Herbert screw treated cases resulted in delayed union. 4. Unions were obtained at average 20 weeks in 3 cases of delayed union. We concluded that open reduction and K-wire fixation were recomanded for the compatible treatment method of displaced waist fracture of scaphoid.

톨루엔 노출 근로자에서 유전자적 요인, 생활습관 및 식이가 요중 마뇨산 배설에 미치는 영향

임현우,박정일,노영만,이강숙,이정민,이원철,이세훈,정치경 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3

목 적 : 본 연구는 생물학적 지표 특성 연구를 위한 단면조사 연구로서 톨루엔 노출과 톨루엔의 생물학적 모니터링으로 이용되는 요중 마뇨산 농도간의 양-반응 관계에서 효과 변경인자로서 작용하는 유전자 ALDH2, CYPIAI, CYP2El의 다양성 분포를 조사하고, 이들 유전자가 요중 마뇨산 배설 농도에 미치는 영향을 알아보고자 하였다. 방 법 : 연구대상자는 경인지역에 위치한 22개의 사업체에서 페인트 제조 72명, 제조업에서 도장작업 50명, 인쇄업 12명, 접착작업 12명, 벽지의 코팅작업 14명등 160명의 근로자이었다. 성, 연령, 유기용제 작업장 근무경력, 흡연습관, 음주습관, 검사일 이전 밤에 음주 여부, 시료 채취전 6시간 이내에 섭취한 식품조사, 측정일 개인보호구의 착용 여부에 대하여 설문조사를 시행하였다. 톨루엔에 대한 개인노출량은 passive air sampler로 측정하였고, 요중 마뇨산은 요중 크레아틴으로 보정하여 측정하였다. 또한 aldehyde dehydrogenase 2 (ALDH2) , cytochrome P45O IAI(CYPIAI), 그리고 cytochrome P45O 2El(CYPfEl)의 유전적 다양성은 혈액으로부터 백혈구의 DNA를 추출하여 제한 효소 절단 길이 다양성 (restiction fragment length polymorphism, RFLP)법으로 검사하여 다음의 결과를 얻었다. 결 과 : 연구대상자의 요중 마뇨산 농도의 기하평균은 0.44g/g creatinine, 기하표준편차 2.80이었다. 톨루엔 노출 농도, 개인 보호구 착용여부, 안식향산이 함유된 식품의 섭취여부중 요중 마뇨산 배설농도에 영향을 미치는 인자는 톨루엔 노출 농도뿐이었다. ALDH2, CYPIAI, CYPfEl, 연령, 흡연, 근무기간에서 회귀계수간에 차이를 보이는 경향이 있었다. 요중 마뇨산 농도에 대한 다중회귀분석에서 톨루엔, ALDH2, CYPIAI, CYP2El유전자형의 회귀계수가 통계적으로 유의하였다. 결 론 : 톨루엔 노출로 인한 요중 마뇨산 배설 농도는 ALDH2, CYPIAI, CYP2El유전자 다양성에 의해 영향을 받기 때문에, 유전자형의 대사능의 차이를 고려하여 마뇨산 기준 농도를 찾는 연구가 이루어져야 하겠다. Objectives The purpose was to investigate the distributions and the effects of genetic polymorphism of aldehyde dehydrogenase 2(ALDH2), cytochrome P45O IAI (CYPIAI), and cytochrome P45O 2El (CYPfEl) on the toluene metabolism. Methods : The subacts consisted of 160 workers who were exposed to toluene in different industries such as paint manufacturing, painting on steel and wood products, printing, bonding, and coating. The exposed toluene level was monitored by passive air sam paler, and the questionnaire variables were age, sex, smoking, drinking, previous night's drinking, use of personal protectlve equipment, work duration, and taking benzoic acid containing food. The urinary hippurric acid collected in the end of shift was corrected by urinary creatinine concentration. The genotypes of ALDH2, CYPIAI, and CYP2El were investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods with DNA extracted from venous blood. Results'The geometric mean and the geometric standard deviation of urinary hippuric acid concentration were 0.44 g/g creatinine and 2.80. The urinary hippuric acid concentration was significantly related to personal exposed toluene level among personal exposed toluene level, use of personal protective equipment, and benzoic acid containing food diet. The slope differences of the regression for ALDH2, CYPIAI, and CYP2E1 genetic polymorphism, age, smoking, and work duration tended to be significant. In multiple regression analysis, the regression coefficient of toluene, ALDH2, 7YPIAl, CYP2E1 genetic polymorphism were significant. Conclusions : From the above results, urinary hippuric acid level after toluene exposure was significantly affected by the genetic polymorphism of ALDH2, CYPIAI, CYPfEl. It is needed further investigation of the urinary hippuric acid level considering the effect of genetic polymorphism.

Hematin의 溶血作用

李康萬 全南大醫大 1970 전남의대학술지 Vol.7 No.4

노인외상환자의 외상중증도계수와 동통감지력의 변화에 대한 임상적 고찰

이진웅,임경수,김영식,황성오,이강현,김선만,김 현 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Because elderly individuals are participitating in social work as adult inspite of their physical handicapes such as hearing difficulty, visual difficulty and limit of motion, they are continually exposed to the risk of injury. Trauma is now fifth cause of death in elderly of over 65 years old, and the elderly suffer disproportionately high injury-related motality comparing with younger adults. In addition, sometimes elderly patients don`t complaint of pain on injured site because of high threshold to pain. We studied about occurence of neglected injury due to high threshold to pain in geriatric trauma patients. When we diagnosised injury that patients did not complaint of pain on injured site through physical examination and radiologic examination, we defined that as neglected injury. There were 8 (8%) geriatric patients who didn`t complaint of pain on injured site on admission to emergency center, but only 0.7% of adult patients had neglected injury. The severity was estimated by use of AIS(Abbreviated Injury Scale), and the severity of all neglected injuries were above AIS 2 point. The neglected injury were head injuries(4 in number), abdominal injury(1), pelvic injuries(2) and extremity injury(1). So if geriatric trauma patients were admitted to emergency center, we recommand full physical and radiological examinations although patients don`t complaint of pain.

Brevibacterium erythrogenes에 의한 스테롤로부터 1,4-Androstadiene-3,17-dione 생성

이은아,이강만 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

한국의 토양으로부터 17-ketosteroid인 ADD를 생성하는 미생물을 분리하여 동정하고 배지조성 및 발효조건을 검토하였다. 집적배양법을 통하여 효소저해제 첨가법에 의해 ADD를 생성할 수 있는 미생물을 분리, 선별하여 9-15라 명명하였다. 이 균주의 형태학적, 생리학적, 생화학적 특성 및 DNA의 T_m을 측정한 결과 Brevibacterium erythrogenes로 동정하였다. ADD 생성의 최적 배지조건 및 발효조건을 검토한 결과 0.1% 콜레스테롤을 함유하는 발효배지에 lactose 0.2%, beef extract 0.2%, bentonite 0.5%를 첨가하고 pH를 7.4로 맞추어 멸균하여 배양 후 18시간에 효소저해제인 α, α´-dipyridyl(1 mM)을 첨가하고 발효시키는 것이 최적 조건임이 검토되었다. 이 조건에 따라 ADD생성을 검토한 결과 27.3%의 전환수율을 얻었으며, 한편 대조실험인 pH7의 0.1% cholesterol 발효배지를 사용하고 배양 후 20시간에 α, α´-dipyridyl을 가하는 배지 및 발효조건으로 배양한 경우는 18.5%의 전환수율을 나타내었다. Microbiological conversion of sterols to 17-ketosteroids has been recongnized as a source for commercal preparation of steroidal drugs. For the purpose of strain development, we isolated microorganisms through enrichment culture method and identified an isolate strain. The strain was closely related to Brevibacterium erythrogenes. The optimal conditions for 1,4-androstadiene-3,17-dione(ADD) formation were as follows; pH 7.4, lactose 0.2%, beef extract 0.2%, bentonite 0.5% in the chlolesterol fermentation medium. Maximum production was obtained with the addition of α, α'-dipyridyl (1 mM, final conc.) at 17-20 hours after incubation.

생쥐 2-세포기 수정란의 초급속동결

강만종,이철상,한용만,유대열,이경광 제주대학교 농과대학 제주도축산문제연구소 1991 畜産論叢 Vol.6 No.1

This study was carried out in order to investigate eggects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos Mouse 2-cell embryos. fol-lowing dehydration by exposure to DMSO and sucrose. were directly immersed into liquid nitrogen and thawed in 37℃ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium a t various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO conentrations (82.6%). However, When sucrose concentrations of 0.25 and 0.5M were added to the freezing medium with 3.0M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time(2.5min) in 3.0M DMSO +0.25M sucrose(85.9%). 3. The development rate of embryos at in vitro 2-celL in vivo 2-celL solution control and untreated control was 84.6%, 90.9%, 89.9%. and 89.7%. respectively.

Brevibacterium erythrogenes에 의한 스테롤로부터 1,4-Androstadiene-3,17-dione 생성

이은아,이강만 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-

한국의 토양으로부터 17-Ketosteroid인 ADD를 생성하는 미생물을 분리하여 동정하고 배지조성 및 발효조건을 검토하였다. 직접배양법을 통하여 효소저해제 첨가법에 의해 ADD를 생성할 수 있는 미생물을 분리, 선별하여 9-15라 명명하였다. 이 규주의 형태학적, 생리학적, 생화화적 특성 및 DNA,의 Tm을 측정한 결과 Brevibacterium erythrogenes로 동정하였다. ADD 생성의 최적 배지조건 및 발효조건을 검토한 결과 0.1% 콜레스테롤을 함유하는 발효배지에 lactose 0.2%, beef extract 0.2%, bentonite 0.5%를 첨가하고 pH를 7.4로 맞추어 멸균하여 배양 후 18시간에 효소저 해제인 a,a'-dipyridyl(1mM)을 첨가하고 발효시키는 것이 최적 조건임이 검토되었다. 이 조건에 따라 ADD 생성을 검토한 결과 27.3%의 전환수율을 얻었으며, 한편 대조실험인 pH7의 0.1% cholesterol발효배지를 사용하고 배양 후 20시간에 a,a'-dipyridyl을 가하는 배지 및 발효조건으로 배양한 경우는 18.5%의 전환수율을 나타내었다. Microbiological conversion of sterols to 17-ketosterods has been recongnized as a source for commerical preparation of steroidal drugs. For the purpose of strain development, we isolated microorganisms through enrichment culture method and identified an isolate strain. The strain was closely related to Brevibacterium erythrogenes. The optimal conditions for 1,4-androstadiene-3,17-dione(ADD) formation were as follows; pH 7.4, lactose 0.2%, beef extract 0.2% bentonite 0.5% in the chloesterol fermentation medium. Maximum production was obtained with the addition of a, a'-dipyridyl (1 mM, final conc.) at 17-20 hours after incubation.

일부 산모에서 산모혈, 제대혈, 모유 및 태반에서의 환경 에스토레겐성 화합물(PCBs) : Environmental estromgen compounds(PCBs) in Maternal Blood, Cord Blood, Human Milk and Placenta of Korea women

이강숙,임현우,노영만,구정완,조재천,박정일,김진홍 가톨릭대학산업의학센타 산업의학연구소 2003 韓國의 産業醫學 Vol.42 No.2

The production and intensive use of organochlorine chemicals, both in agriculture and industry, have led to wide spread contamination of the environment. There may be biological effects of organochlorine contaminated breast milk even when levels are below those concentrations that are currently regarded as tolerable. To evaluate the exposure of to fetus PCBs, we measured the sum of 13 congeners of PCBs in maternal blood, cord blood, breast milk and placenta of 30 Korean women by gas chromatograpy/electron capture detector. The concentration of total PCBs of maternal blood, cord blood and breast milk were 7.16±4.29 ㎍/ℓ, 5.16±3.12 ㎍/ℓ, 1.77±2.87 ㎍/ℓ respectively and they have no significant correlation. PCBs in placenta was not defected. However there were no significant differences of PCBs according to age, education, body mass index, parity, breastfeeding, smoking and drinking.

Actinoplanes ferrugineus 세포추출액에 의한 Methylthioadenosine 대사중간생성물 확인

이강만,김유석 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.46 No.-

To identify intermediate(s) during the conversion of methylthioadenosine to methionine by cell-free extracts of Actinoplanes ferrugineus, a compound was isolated from the anaerobic incubation mixture of cell-free extracts and CH_3-[^14C]-methylthioadenosine, followed by 0.01 N―HCI hydrolysis. The compound was characterized through alkaline phosphatase treatment, NaBH_4 reduction, and NaBH_4 reduction―periodate oxidation reaction. The intermediate seemed to be 1-phospho-5-methylthio-5-deoxyribulose.

Actinoplanes ferrugineus 세포추출액에 의한 Methylthioadenosine 대사중간생성물 확인

이강만,김유석 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

To identify intermediate(s) during the conversion of methylthioadenosine to methionine by cell-free extracts of Actinoplanes ferrugineus, a compound was isolated from the anaerobic incubation mixture of cell-free extracts and CH_3-[^14C]-methylthioadenosine, followed by 0.01 N-HCI hycrolysis. The compound was characterized through alkaline phosphatase treatment, NaBH_4 reduction, and NaBH_4 reduction-periodate oxidation reaction. The intermediate seemed to be 1-phospho-5-methylthio-5-deoxyribulose.