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( Sang Hyun Lee ),( Seonki Lee ),( Kibaek Lee ),( Chang Hyun Nahm ),( Sung-jun Jo ),( Jaewoo Lee ),( Kwang-ho Choo ),( Jung-kee Lee ),( Chung-hak Lee ),( Pyung-kyu Park ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
Quorum quenching (QQ) bacteria entrapped in a polymeric composite hydrogel (QQ medium) have been successfully applied in membrane bioreactors (MBRs) for effective biofouling control. However, in order to bring QQ technology closer to practice, the physical strength and lifetime of QQ media should be improved. In this study, enforcement of physical strength, as well as an extension of the lifetime of a previously reported QQ bacteria entrapping hollow cylinder (QQ-HC), was sought by adding a dehydration procedure following the cross-linking of the polymeric hydrogel by inorganic compounds like Ca<sup>2+</sup> and boric acid. Such prepared medium demonstrated enhanced physical strength possibly through an increased degree of physical cross-linking. As a result, a longer lifetime of QQ-HCs was confirmed, which led to improved biofouling mitigation performance of QQ-HC in an MBR. Furthermore, QQ-HCs stored under dehydrated condition showed higher QQ activity when the storage time lasted more than 90 days owing to enhanced cell viability. In addition, the dormant QQ activity after the dehydration step could be easily restored through reactivation with real wastewater, and the reduced weight of the dehydrated media is expected to make handling and transportation of QQ media highly convenient and economical in practice.
Lee, Jaewoo,Won, Young-June,Choi, Dong-Chan,Lee, Seonki,Park, Pyung-Kyu,Choo, Kwang-Ho,Oh, Hyun-Suk,Lee, Chung-Hak Elsevier 2019 Journal of membrane science Vol.592 No.-
<P><B>Abstract</B></P> <P>In this study, a patterned quorum quenching (QQ) membrane with anti-biofouling characteristics was prepared <I>via</I> a patterning process of an oxidized multiwall carbon nanotube (o-MWCNT) composite membrane followed by immobilization of QQ enzymes. We verified that the o-MWCNTs with a high specific surface area helped the patterned membrane retain 32% more enzyme compared to the case without the o-MWCNTs. Furthermore, the micro-pattern on the membrane surface increased the effective membrane area (~1.6 times), offsetting the membrane resistance additionally caused by enzyme immobilization. The patterned QQ membrane kept the relative activity at above 40% of the initial activity for 39 days due to the cross-linking. It was also confirmed in a flow cell test that the patterned QQ membrane had much more control over biofilm formation by effectively disrupting quorum sensing compared to the patterned membrane. Due to the combined effect of the QQ activity and the pattern effect (<I>e.g.</I>, low permeation drag), the patterned QQ membrane (2.2 times) significantly lengthened the time between chemical cleanings compared to the non-patterned membrane. The combined effect also reduced the specific filtration energy consumption of the patterned QQ membrane to less than half of that of the non-patterned membrane as the two membranes were used to produce the same amount of the treated water during the same period. We expect that the patterned QQ membrane will open and facilitate interdisciplinary research combining patterned membranes with technologies in other areas to improve the antifouling properties of patterned membranes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Interdisciplinary research was conducted to prepare the patterned QQ membrane. </LI> <LI> The patterned QQ membrane effectively suppressed the biofilm formation by QQ effect. </LI> <LI> The micro-pattern offset membrane resistance caused by enzyme immobilization. </LI> <LI> The patterned QQ membrane remarkably prolonged the time between chemical cleanings. </LI> <LI> The patterned QQ membrane consumed significantly less specific filtration energy. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Siyoung,Kim, Eunsom,Jhun, Hyunjhung,Hong, Jaewoo,Kwak, Areum,Jo, Seunghyun,Bae, Suyoung,Lee, Jongho,Kim, Busun,Lee, Jungmin,Youn, Sulah,Kim, Somi,Kim, Miyeon,Kim, Hyunwoo,Lee, Youngmin,Choi, Dong American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.28
<P>Although it has been established that diabetes increases susceptibility to infections, the role of insulin (INS) in the immune response is unknown. Here, we investigated the immunological function of INS. Proinsulin dimer (pINSd) was a potent immune stimulus that induced inflammatory cytokines, but mature INS was unable to induce an immune response. An affinity-purified rabbit polyclonal antibody raised against mature IL-1α recognized IL-1α and pINS but failed to detect mature INS and IL-1β. Analysis of the pINS sequence revealed the existence of an INS/IL-1α motif in the C-peptide of pINS. Surprisingly, the INS/IL-1α motif was recognized by monoclonal antibody raised against IL-1α. Deleting the INS/IL-1α motif in pINSd and IL-1α changed their activities. To investigate the pINSd receptor, the reconstitution of IL-1 receptor 1 (IL-1R1) in Wish cells restored pINSd activity that was reversed by an IL-1R antagonist. These data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1.</P>
Lee, Jaewoo,Jang, Jun Hee,Chae, Hee-Ro,Lee, Sang H.,Lee, Chung-Hak,Park, Pyung-Kyu,Won, Young-June,Kim, In-Chul The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.44
<▼1><P>Incorporation of single-layer graphene oxide into a highly porous support layer provides a thin-film composite reverse osmosis membrane with superior water flux.</P></▼1><▼2><P>In this study, we demonstrated that a reduction in solely the concentration of the polymer solution for preparation of the support layer effectively enhances the water flux of a thin-film composite (TFC) reverse osmosis (RO) membrane. However, a decrease in the polymer concentration caused the sub-surface structure of the support layer to become too porous, which unavoidably weakened the mechanical strength of the support layer. To overcome the problem, we prepared a highly porous support layer with improved mechanical strength by incorporating graphene oxide (GO) platelets. The thickness of the GO platelets was controlled by adjusting the mechanical energy input per volume of the precursor solution. We confirmed that well-exfoliated GO platelets (mean thickness: about 1.5 nm) are more effective in enhancing the mechanical properties of the support layer. The TFC RO membrane made of the GO composite support layer had almost 1.6 to 4 times higher water flux with comparable salt rejection compared to both the current upper bounds of the RO membranes prepared by modification of the active layer and commercial RO membranes.</P></▼2>
Lee, Myunggyo,Lee, Kyubum,Yu, Namhee,Jang, Insu,Choi, Ikjung,Kim, Pora,Jang, Ye Eun,Kim, Byounggun,Kim, Sunkyu,Lee, Byungwook,Kang, Jaewoo,Lee, Sanghyuk Oxford University Press 2017 Nucleic acids research Vol.45 No.d1
<P>Fusion gene is an important class of therapeutic targets and prognostic markers in cancer. ChimerDB is a comprehensive database of fusion genes encompassing analysis of deep sequencing data and manual curations. In this update, the database coverage was enhanced considerably by adding two new modules of The Cancer Genome Atlas (TCGA) RNA-Seq analysis and PubMed abstract mining. ChimerDB 3.0 is composed of three modules of ChimerKB, ChimerPub and ChimerSeq. ChimerKB represents a knowledgebase including 1066 fusion genes with manual curation that were compiled from public resources of fusion genes with experimental evidences. ChimerPub includes 2767 fusion genes obtained from text mining of PubMed abstracts. ChimerSeq module is designed to archive the fusion candidates from deep sequencing data. Importantly, we have analyzed RNA-Seq data of the TCGA project covering 4569 patients in 23 cancer types using two reliable programs of FusionScan and TopHat-Fusion. The new user interface supports diverse search options and graphic representation of fusion gene structure. ChimerDB 3.0 is available at http://ercsb.ewha.ac.kr/fusiongene/.</P>
Lee, Young-Kwan,Lee, Hoon Bok,Shin, Dong-Mi,Kang, Min Jueng,Yi, Eugene C,Noh, Seungjoo,Lee, Jaewoo,Lee, Chulbom,Min, Chang-Ki,Choi, Eun Young Nature Publishing Group 2014 Experimental and molecular medicine Vol.46 No.11
<P>Indoleamine 2,3-dioxygenases (IDOs) are tryptophan-catabolizing enzymes with immunomodulatory functions. However, the biological role of IDO2 and its relationship with IDO1 are unknown. To assess the relationship between IDO2 and IDO1, we investigated the effects of co-expression of human (h) IDO2 on hIDO1 activity. Cells co-expressing hIDO1 and hIDO2 showed reduced tryptophan metabolic activity compared with those expressing hIDO1 only. In a proteomic analysis, hIDO1-expressing cells exhibited enhanced expression of proteins related to the cell cycle and amino acid metabolism, and decreased expression of proteins related to cell survival. However, cells co-expressing hIDO1 and hIDO2 showed enhanced expression of negative regulators of cell apoptosis compared with those expressing hIDO1 only. Co-expression of hIDO1 and hIDO2 rescued the cell death induced by tryptophan-depletion through hIDO1 activity. Cells expressing only hIDO2 exhibited no marked differences in proteome profiles or cell growth compared with mock-transfectants. Cellular tryptophan metabolic activity and cell death were restored by co-expressing the hIDO2 mutant substituting the histidine 360 residue for alanine. These results demonstrate that hIDO2 plays a novel role as a negative regulator of hIDO1 by competing for heme-binding with hIDO1, and provide information useful for development of therapeutic strategies to control cancer and immunological disorders that target IDO molecules.</P>
이재우 ( Jaewoo Lee ),이동철 ( Dongcheul Lee ),신치범 ( Chee Burm Shin ),이소연 ( So-yeon Lee ),오승미 ( Seung-mi Oh ),우중제 ( Jung-je Woo ),장일찬 ( Il-chan Jang ) 한국화학공학회 2021 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.59 No.3
리튬이온전지의 성능을 최적화하기 위해서는 여러 열화 요소들을 고려한 성능 예측 모델링 기술이 필요하다. 본 연구에서는 리튬이온전지의 사이클 노화로 인한 방전 거동 및 사이클 수명 변화를 수학적으로 모델링하였다. 모델링의 신뢰성을 검증하기 위해 0.25C로 사이클 시험을 진행했으며, 30 사이클 간격으로 진행한 RPT (Reference performance test)를 통해 전기적 거동을 파악하였다. 기존의 리튬이온전지의 사이클 수명 예측 모델에 BOL (Beginning of life)에서 일어나는 현상 중 하나인 Break-in 메커니즘을 반영하여 수명예측 정확도를 개선시켰다. 모델에 근거하여 예측된 사이클 수명 변화는 실제 시험 결과와 잘 일치하였다. In order to optimize the performance of a lithium-ion battery, a performance prediction modeling technique that considers various degradation factors is required. In this work, mathematical modeling was carried-out to predict the change in discharging behavior and cycle life, taking into account the cycle aging of lithium-ion batteries. In order to validate the modeling, a cycling test was performed at the charge/discharge rate of 0.25C, and discharging behavior was measured through RPT (Reference Performance Test) performed at 30 cycle intervals. The accuracy of cycle life prediction was improved by considering the break-in mechanism, one of the phenomena occurring in the BOL (beginning of life), in the model for predicting the cycle life of lithium-ion batteries. The predicted change in cycle life based on the model was in good agreement with the experimental results.
외란 추정 기반 Steer-by-Wire 시스템의 고장 진단에 대한 연구
이재우(Jaewoo Lee),원종천(Jongchun Won),이창희(Changhee Lee),표종현(Jonghyun Pyo),이진환(Jinhwan Lee) 한국자동차공학회 2022 한국자동차공학회 부문종합 학술대회 Vol.2022 No.6
In the steering system, faults can be caused by increased and decreased internal friction due to leaks, corrosion, wear etc. An abnormal increase in friction causes problems such as the steering wheel becomes heavy, which increases the drivers steering effort, and the steering wheel does not return to the origin when returning. In addition, when the friction is abnormally low, the fatigue of the mechanical parts increases and there is a risk of damage. In this paper, a method for diagnosing faults in steer-by-wire(SbW) systems based on friction index is proposed. Friction index is estimated using disturbance generated by operating a specific scenario through rack position control. The scenario is tested by changing the amplitude, speed, and temperature based on the speed at which a general driver moves in the on-center area. The disturbance force is estimated using the Kalman filter.