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충청남도 해안지역의 비브리오 패혈증균 분포 및 분리주의 특성 분석
이현아(Hyunah Lee),고영은(Young-Eun Ko),최지혜(Jihye Choi),이다연(Dayeon Lee),여성순(Seoungsoon Yeo),박종진(Jongjin Park),이미영(Miyoung Lee),최진하(Jinha Choi),박준혁(Junhyuk Park) 한국환경보건학회 2021 한국환경보건학회지 Vol.47 No.5
Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.
Lee, Hyunah,Ha, Min Jung,Shahbaz, Hafiz Muhammad,Kim, Jeong Un,Jang, Holim,Park, Jiyong Elsevier 2018 Journal of food engineering Vol.238 No.-
<P><B>Abstract</B></P> <P>The traditional process of manufacturing red bean powder (RBP) using high temperature and extensive cooking causes quality changes, such as an off-flavor, destruction of colors, and a reduced nutritional value of the product. In this study, RBP was manufactured using a novel process of wet grinding, high hydrostatic pressure (HHP), and freeze-drying. The impact of HHP treatment in this innovative manufacturing process on the microbial count, antioxidant activity, anti-nutritional factors, and physicochemical properties of RBP was evaluated by comparison with the conventional method of thermal treatment. Microbial populations in final RBP were reduced to 1.83, 1.55, and 1.05 log CFU/g when treated using HHP 400, 500, and 600 MPa, respectively. HHP treatment improved the nutritional profile and reduced the effects of anti-nutritional factors in RBP. The most effective reduction of trypsin inhibitor activity and phytic acid content was achieved using HHP at 600 MPa for 5 min. Total phenols, flavonoids, proanthocyanidins and total antioxidant capacity in HHP treated RBP were more effectively preserved than in thermally treated RBP. Preparation of RBP using a novel manufacturing process involving HHP treatment rather than a thermal treatment improved physico-chemical and functional properties and enhanced microbiological safety.</P> <P><B>Highlights for review</B></P> <P> <UL> <LI> Red bean powder was manufactured using a novel process. </LI> <LI> Process included wet grinding, high hydrostatic pressure (HHP), and freeze-drying. </LI> <LI> HHP effectively disinfected naturally occurring microorganisms in red bean powder. </LI> <LI> Nutritional and functional properties of red bean powder were effectively preserved. </LI> </UL> </P>
Lee, Junghee,Hwang, Yu Jin,Kim, Yunha,Lee, Min Young,Hyeon, Seung Jae,Lee, Soojin,Kim, Dong Hyun,Jang, Sung Jae,Im, Hyoenjoo,Min, Sun-Joon,Choo, Hyunah,Pae, Ae Nim,Kim, Dong Jin,Cho, Kyung Sang,Kowall Springer Verlag 2017 Acta neuropathologica Vol.134 No.5
<P>Huntington's disease (HD) is an autosomal-dominant inherited neurological disorder caused by expanded CAG repeats in exon 1 of the Huntingtin (HTT) gene. Altered histone modifications and epigenetic mechanisms are closely associated with HD suggesting that transcriptional repression may play a pathogenic role. Epigenetic compounds have significant therapeutic effects in cellular and animal models of HD, but they have not been successful in clinical trials. Herein, we report that dSETDB1/ESET, a histone methyltransferase (HMT), is a mediator of mutant HTT-induced degeneration in a fly HD model. We found that nogalamycin, an anthracycline antibiotic and a chromatin remodeling drug, reduces trimethylated histone H3K9 (H3K9me3) levels and pericentromeric heterochromatin condensation by reducing the expression of Setdb1/Eset. H3K9me3-specific ChIP-on-ChIP analysis identified that the H3K9me3-enriched epigenome signatures of multiple neuronal pathways including Egr1, Fos, Ezh1, and Arc are deregulated in HD transgenic (R6/2) mice. Nogalamycin modulated the expression of the H3K9me3-landscaped epigenome in medium spiny neurons and reduced mutant HTT nuclear inclusion formation. Moreover, nogalamycin slowed neuropathological progression, preserved motor function, and extended the life span of R6/2 mice. Together, our results indicate that modulation of SETDB1/ESET and H3K9me3-dependent heterochromatin plasticity is responsible for the neuroprotective effects of nogalamycin in HD and that small compounds targeting dysfunctional histone modification and epigenetic modification by SETDB1/ESET may be a rational therapeutic strategy in HD.</P>
Hyunah Park,Joonhwan Lee,Sangsuk Lee 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2
Mobile remote presence (MRP) robots allow physically remote people to interact and communicate as if they are in the same place. MRP function as embodied proxy agents. In this study, we tried to examine the effect of MRP robots on quality of communication and life satisfaction for institutionalized elderly people. They usually have a mild cognitive impairment, which decreases their ability to communicate with people and will to live lively. We assumed that communicating via MRP robots would improve their quality of communication and life satisfaction through the sense of presence and mobility. To test this assumption, we conducted two experiments-involving 1) three communication conditions (telephone, video call, and MRP) to examine quality of communication, and 2) six tasks to test the perception of a presence and life satisfaction. Our results showed that quality of communication was highest in the MRP condition for most of the participants. Also, interacting with the MRP significantly increased life satisfaction, although no significant effect was found on perceived presence.
Hyunah Lim,Joon Hyuck Jang,Sanghyu Nam,Koeun Lee,Jae Yong Kim,Hungwon Tchah,Hun Lee 대한안과학회 2024 Korean Journal of Ophthalmology Vol.38 No.1
Purpose: We aimed to compare the accuracy of the intraocular lens (IOL) calculation formula using the standard keratometry (K) and total K (TK) during the femtosecond laser-assisted cataract surgery (FLACS) with a monofocal IOL with enhanced intermediate function using currently used formulas. Methods: A retrospective review of 125 eyes from 125 patients who had undergone FLACS with implantation of monofocal IOL with enhanced intermediate function was conducted. The predicted refractive power was calculated using an optical biometer (IOLmaster 700) according to the K and TK in the Barrett Universal II, SRK/T, Haigis, and Holladay 2 formulas. Absolute prediction error (APE) obtained from the actual postoperative refractive outcomes and the refractive error predicted in each formula was compared one month after surgery. Results: Mean APE ranged between 0.29 and 0.39 diopters (D) regardless of the calculation formula and the method of measuring corneal curvature. Significant differences were observed in the APE from the four formulas and the two keratometric measurements (p = 0.014). In a total of 125 eyes from 125 patients, the mean APE was lowest with the Barrett Universal II formula. Across all formulas, both the mean APE and the median APE tended to be lower for K than for TK, although there was no significant difference. Approximately 70% to 80% of the patients were included within 0.5 D of the refractive error across all formulas. The percentage of eyes within 0.5 D of APE outcomes was not statistically different between the K and TK data when using each formula. Conclusions: Keratometric measurements considering the poster corneal curvature did not show any additional advantages when implanting the monofocal IOL with enhanced intermediate function during the FLACS.
Lee, Hyunah,Shahbaz, Hafiz Muhammad,Ha, Namho,Kim, Jeong Un,Lee, Sang Jun,Park, Jiyong The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: Korean ginseng (Panax ginseng Meyer) powder is in rising demand because powder forms of foods are convenient to handle and are highly preservable. However, ginseng powder (GP) manufactured using the conventional process of air drying and dry milling suffers nutrient destruction and a lack of microbiological safety. The objective of this study was to prepare GP using a novel process comprised of UV-TiO<sub>2</sub> photocatalysis (UVTP) as a prewashing step, wet grinding, high hydrostatic pressure (HHP), and freeze-drying treatments. Methods: The effects of UVTP and HHP treatments on the microbial population, ginsenoside concentration, and physiological characteristics of GP were evaluated. Results: When UVTP for 10 min and HHP at 600 MPa for 5 min were combined, initial 4.95 log CFU/g-fw counts of total aerobes in fresh ginseng were reduced to lower than the detection limit. The levels of 7 major ginsenosides in UVTP-HHP-treated GP were significantly higher than in untreated control samples. Stronger inhibitory effects against inflammatory mediator production and antioxidant activity were observed in UVTP-HHP-treated GP than in untreated samples. There were also no significant differences in CIELAB color values of UVTP-HHP-treated GP compared with untreated control samples. Conclusion: Combined processing of UVTP and HHP increased ginsenoside levels and enhanced the microbiological safety and physiological activity of GP.
Lee, Mijin,Shahbaz, Hafiz Muhammad,Kim, Jeong Un,Lee, Hyunah,Lee, Dong-Un,Park, Jiyong Elsevier 2018 FOOD MICROBIOLOGY Vol.76 No.-
<P><B>Abstract</B></P> <P>Surface disinfection of fresh blueberries is an important food safety challenge due to the delicate texture and short shelf life of these small fruits. A newly designed water-assisted photocatalytic reactor was developed for disinfection of fruits with a delicate texture and complex surface characteristics. Efficacy of UV-TiO<SUB>2</SUB> photocatalysis was evaluated in comparison with UV alone for inactivation of <I>Escherichia coli</I> K12 (as a surrogate for <I>Escherichia coli</I> O157:H7) inoculated onto the surface of the blueberry skin, calyx, and an experimentally prepared agar matrix that was used as a model matrix. Influence of surface characteristics such as surface hydrophobicity and surface free energy on bacterial adhesion were also investigated. The initial bacterial population on all surfaces was approximately 7.0 log CFU/g. UV-TiO<SUB>2</SUB> photocatalysis (4.5 mW/cm<SUP>2</SUP>) for 30 s achieved comparatively higher bacterial reductions of 5.3 log and 4.6 log CFU/g on blueberry skin and agar matrix surfaces, respectively, than 4.5 log and 3.4 log CFU/g reductions for UV alone (6.0 mW/cm<SUP>2</SUP>). Total phenolic and total anthocyanin contents of fruits were significantly increased after both UV-TiO<SUB>2</SUB> and UV treatments, compared with water washed control fruits. UV-TiO<SUB>2</SUB> photocatalysis technology is a non-chemical and residue-free method with reduced water usage for surface disinfection of fresh blueberries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Photocatalysis method was developed for disinfection of fruits with a delicate texture. </LI> <LI> UV-TiO<SUB>2</SUB> photocatalysis was effective against <I>E. coli</I> K12 on blueberry fruit surface. </LI> <LI> Surface properties of blueberry skin and calyx influenced the disinfection efficiency. </LI> <LI> Photocatalysis is a non-chemical and residue-free method with reduced water usage. </LI> </UL> </P>