Duchenne형 進行性 筋異養症 保因者 檢出에 Restriction Fragment Length Polymorphism 이용에 관한 硏究

李美正,朴吉洪,朴善華,柳總根 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.3

We performed genetic analysis for detection of carrier state of Duchenne's muscular dystrophy (DMD). As genetic markers of the disorder, We used DNA-sequence polymorphisms detected with 6 different DNA probes derived from the vicinity of the DMD locus at genetic distance less than 15 cmo or from within the gene. on the short arm of X chromosome. In the family we studied. a consultand was a 29 years old woman whose maternal grandfather was a DMD patient who was unavailable. Therefore, all of her maternal aunts were DMD carriers whose next generations were requested for DNA studies. On the basis of linkage analysis. a proband and her niece were deemed to be unaffected with an accuracy of more than 99%, and we anticipated another two femals and one malt to be unaffected with 98% probability (normal serum creatine kinase level) where crossover events between intragenic(pERT 87.30) and flanking probes(pXUT23) were detected. but carrire risk of a female with the same haplotype was elevated to 41% taking account of an increased serum creatine kinase level. Two other females were predicted to be affected with 99.9 and 97.1% probabilities each. However. a genetic interpretation was impossible for one male in which a crossover breakpoint could not be determinied because of his mother's homozygosity of the alleles between crossovered alleles where hitherto pessibility of intragenic crossover could not be ruled out Our experience suggests that genetic diagnosis based on recombinant deoxyribonucleic acid techniques are assuming increasing importance. and provide a highly accurate method for carrier detection and prenatal diagnosis of DMD. By the way, despite the large number of intragenic and flanking DNA polymorhpisms available, uncertainties often remain in the genetic diagnosis of DMD. Pitfalls are presented by the large size of the region in which Duchenne's mutations can occur. Crossover events in this region. especially intragenic ones, which result in an exchange of DNA between two X chromosomes, can,rener DNA-marker studies inaccurate.

매자나무科 植物의 Alkaloid 硏究 (I) : 매자나무의 硏究

李容柱,李聖圭,李宗健 成均館大學校 1967 論文集 Vol.12 No.-

民間에서 苦味健胃藥 또는 洗眼劑로 使用되는 매자나무의 地上莖에서 第三級?基로서 berbamine, oxyberberine, 第四級?基로서 jatrorrhizine, magnoflorine, berberine, shobakunine等을 分離確認하고, 1個의 未知?基를 分水하였다. 地下莖의 ?基는 地下莖와 같으나 上記 末知?基가 없었고 第三級?基인 oxyacauihiu이 存在하였다. Examinations were made on the alkaloids contained in the Berberis Koreana Palibin (Korean name, Maeza Namoo) and 8 kinds of alkaloids were isolated. By the various physico-chemical methods, the presences of oxyacanthin and berbamin as the tert-biscoclaurin-type base, oxyberberin as the berberin-type base and a unknown base were proved. Water-soluble, quaternary bases present were also proved as the berberin-type, berberin jatrarrhizin, shobakunin, and aporphin-type magnoflorin.

Carcinogen에 依한 白鼠 肝癌組織內 γ-Glutamyltransferase에 관한 硏究

李春魯,朴鈗圭,柳總根 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

The purpose of this study is to investigate the physicochemical characteristics and isozyme pattern of y-glutamyltransferase (γ-GT) in hepatocellular carcinoma. Employing wister albino rat as subject, γ-GT was purified from hepatocellular carcinoma (HCC) induced by 2-acetamidofluorene (AAF) as carcinogen, regenerative liver tissue after administration of AAF for 4 weeks (AAF-4), and normal adult liver (control) to observe molecular weight, optimum pH, optimum temperature, heat stability, and isozyme pattern on polyacrylamide gel electrophoresis. Results obtained are as follows. 1. γ-GT of HCC was purified 59 fold with and yield of 24%. The final specific activity obtaine was 23.2 unit per mg of protein. 2. Sialo form was seen to be included only in γ-GT of HCC on Con A-Sepharose chromatography. 3. The molecular weight of sialo form and asialo form γ-GT of HCC were determined as 120,000 and 110,000 each. 4. The optimum pH and temperature of γ-GT from all the experimental group were 9.0 and 50℃ respectively. 5. Heat stability of γ-GT was tested, and found to be stable at 37℃ and exhibited denaturation at temperature above 50℃ γ-GT of HCC being most remarkable compared to that of control group and regenerative tissue (AAF-4). 6. K_m value of γ-GT for γ-glutamyl-P-nitroanilide was 1.95x10 exp (-3) M in control group and 1.23x10 exp (-3) M in HCC. 7. On polyacrylamide gel electrophoresis, Γ-GT of control group displayed one fraction, AAF-4, two fractions, and that of HCC three fractions.

단일클론 항 γ-Glutamyltrasferase(GCT) 항체를 이용한 혈뇌장벽 내 GGT의 분포에 관한 연구

이병규,김명곤,신규만,류총근 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.3

γ-Glutamyltransferase(GGT: E.C. 2.3.2.2.) is a glycoprotein enzyme which is involved in glutathione metabolism and amino acid transport through the plasma membrane. It is distributed widely in several organs including liver, kidney, pancrease and brain. GGTs derived from the brain of Wister rats and BALB/c mice were biochemically purified to a specific activity of 4246.2, 862.1 units per mg of protein, a purification folds 93.7, 43.8 and the final yield 65.8, 44.0% respectively. Electrophoretic pattern of purified GGTs from rats and mice brain shows very similar protein fraction each other. We have produced six monoclonal antibodies(GGT-MAb 1-6) against 2-acetamidofluorene treated rat liver GGT. Using these GGT-MAb 1-6 we performed immunohistochemistry(IHC) to study the distribution of GGY isozymes in normal tissues of rat brain and in ncoplastic tissues of human brain. The results indicated that human brain GGT was localized in pericytes of blood-brain barrier, especially in the blood-rich portion of the brain(e.g. cerebellum of rat, meningioma and craniopharyngioma of human). Therefore these MAbs may be used to evaluate the distribution of GGT isozymes in different tissues.

防波堤의 任意 配置에 對한 波浪 廻折

李한배,李庸揆,吳世汎,片宗根 명지대학교 공학기술연구소 1988 공학기술연구소 논문집 Vol.3 No.-

In harbor design, it is a necessary condition to retain calmness inside a harbor. For this purpose, breakwaters are disposed as exterior structures against incident waves. There are cases that necessitate main and auxiliary breakwaters according to frequent directions of incident waves. In these cases, harbor entrances have various geometric shapes in horizontal plane. In the program developed here, harbor entrances are classified into six different cases by the disposition of breakwaters and the attack angle of incident waves. For each case, the diffraction phenomena inside and around harbor entrance are analysed by superposing the solutions of Sommerfeld's. The method gives fairly good results compared with those reported before, and also is proved to be computationally very efficient. Therefore, the method may be applicable to the preliminary design of breakwater dispositions, and also the program can be used as the basic subroutine in the calculation of wave height distributions inside a harbor with reflected waves.

측두부에 발생한 Cranial Fasciitis의 치험례

정현권,변준희,이종원,이종건,임풍,박성찬 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Cranial fasciitis is a rare morphological variant of nodular fasciitis. It is characterized by a rapid growing fibroblastic proliferative lesion that develops chiefly in childhood. It has varying size and involves the soft tissues of the scalp and the underlying skull. Accurate diagnosis and surgical excision is the key to management. Prognosis is good with rare recurrence. We report a case of cranial fasciitis in the temporal fossa of a 20-month-old girl and present a review of the literature.

Takayama 방법에 의한 항내 파고 분포 계산

이해종,편종근 명지대학교 공학기술연구소 1989 공학기술연구소 논문집 Vol.4 No.-

In designing a new harbor or expanding and modifying the port facilities, one of the great concerns is how to reduce the disturbance of water inside a harbor. The calmness may be influenced by many external factors such as waves, winds, and currents, in which waves are the most influential. Waves are composed of incident wave, reflected wave, transmitted wave, ship wave, wind generating wave and long period oscillations. Among them, incident wave and reflected wave are most important to estimate the wave height distributions around a harbor. In this paper, wave height distributions in HUPO harbor is calculated with numerical model, which are Takayama's model, superposing Sommerfeld's solution. The validities of these model is also investigated by comparisons with experimental results. It is shown that the computational results of Takayama's model agree reasonably with the experimental values.

Mathieu 函數를 利用한 波浪의 廻折에 관한 硏究

片宗根,李庸揆,金鐵 명지대학교 공학기술연구소 1987 공학기술연구소 논문집 Vol.2 No.-

Calmness in water condition inside a harbor is a great concern for port engineers. Therefore it is necessary to predict wave diffraction in a harbor reasonably. Available diffraction diagrams for wave diffraction through a breakwater gap or beyond an island breakwater are less satisfactory and still incomplete. In this paper, as a fundamental study, the programs to compute wave diffractions through a breakwater gap or by an island breakwater are developed by using the Mathieu functions. The wave diffraction diagrams in the dimensionless wiegel format, drawn from these programs, can be used well for preliminary design purposes.

Cs이 흡착된 Si(111)7×7표면에 대한 RHEED연구

박종윤,이순보,이경원,안기석,강건아 성균관대학교 1991 論文集 Vol.42 No.1

Cesium-adsorbed surface structures on Si(111)7×7 were investigated at room and high temperatures(200∼700℃) by RHEED. The RHEED patterns of Si(111) 7×7 was changed to the modified 7×7 and the 1×1 patterns with increasing the deposition times of Cs at RT. It was observed that the structure of the Cs-adsorbed Si (111) 7×7 surface at saturation coverage is the 1×1 structure at RT. The ◁그림삽입▷(원문을 참조하세요) 1 and 1×1 structures appeared successively at the adsorption temperature of 300℃, 350∼400℃ and 450℃, respectively. After subsequent heating of 1×1 surface above 550℃ and of ◁그림삽입▷(원문을 참조하세요) surface above 600℃, each RHEED pattern gradually returned to the original Si(111)7×7 pattern. 상온 및 200∼700℃의 Si(111)7×7 표면에 Cs(Cesium)을 증착하였을 때 표면격자구조의 변화를 RHEED로 관측하였다. Cs 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 포화 덮임률에 도달했다고 추정되는 일정 증착시간 전에는 원래의 깨끗한 Si(111)7×7 패턴과 거의 유사한 변형된(modified) Si(111)7×7-Cs 패턴이 관측되었다. 그후 포화 덮임률에서는 1×1패턴이 관측되고 증착량을 증가시켜도 패턴의 변화는 관측되지 않았다. 이 구조를 다시 annealing시키면 약 550℃부터 서서히 원래의 7×7구조로 되돌아가기 시작한다. Si(111)7×7기판의 온도를 220∼700℃로 유지하면서 Cs을 증착시킨 경우에 약 250℃까지는 상온에서와 비슷한 변형된(modified) 7×7이 관측되고 약 300℃에서는 ◁그림삽입▷(원문을 참조하세요) 350∼400℃ 정도에서는 ◁그림삽입▷(원문을 참조하세요)과 3×1이 겹쳐셔 관측되었다. 그리고 450℃ 이상에서는 1×1구조가 관측되었다. 이때 약 300℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 350℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 3+3×1 구조는 약 500℃ 정도까지 다시 annealing함에 따라 다리 1×1구조로 상전이가 일어난후, 약 600℃부터 원래의 7×7의 초격자점들이 나타나기 시작했다. 이들 결과로부터 Si(111)7×7 표면에 Cs을 증착하는 경우에는 일정한 포화 덮임률(saturation coverage)이 있는 것으로 추정되고, 이 덮임률에서 관측된 고온에서의 상전이는 증착량(증착시간)에는 무관하고 온도에만 의존함을 알 수 있었다. 또한 1×1 구조와 ◁그림삽입▷(원문을 참조하세요) 구조에 대하여 Cs의 탈착은 각각 약 550℃와 600℃에서 일어나기 시작하여 700℃에서 완전히 탈착됨을 알 수 있었다.

K이 흡착된 Si(111)7×7표면에 대한 RHEED 연구

박종윤,이순보,이경원,안기석,신익조,강건아 성균관대학교 1990 論文集 Vol.41 No.2

상온 및 200℃∼600℃의 Si(111) 7×7 표면에 칼륨(K)을 증착하였을 때의 표면격자구조 변화를 RHEED로 관측하였다. K 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 어느 일정한 증착시간(포화덮임률에 도달했다고 추정되는 증착시간)이 지난 후에는 원래의 깨끗한 Si(111)7×7 패턴과 유사한 Si(111) 7×7-K 패턴이 관측되었고, 증착시간을 증가시켜도 RHEED 패턴은 변화하지 않았다. 이것을 annealing하면 350℃까지는 RHEED 패턴에 변화가 없다가 그 이상의 온도가 되면 서서히 원래의 7×7 패턴으로 되돌아가기 시작한다. Si(111) 7×7기판의 온도를 200℃∼600℃로 유지하면서 K을 일정시간(450℃에서 3×1이 형성되는 증착시간)이상 증착시킨 경우에 250℃까지는 상온의 경우와 비슷한 형태의 변형된 7×7 패턴이 관측되고, 300℃∼550℃일때는 3×1, 550℃ 이상에서는 1×1 구조가 관측되었다. 이때 300℃∼550℃에서 형성된 Si(111) 3×1-K 구조는 450℃에서 1분 정도 annealing 하면 항상 Si(111) 1×1-K 구조로 상전이가 일어남을 관측하였다. Potassium adsorbed surface structures of Si(111) 7×7 surface at room and high temperatures(200℃∼600℃) were investigated by RHEED. Potassium adsorption on the Si(111) 7×7 surface to saturated coverage at room temperature changed the RHEED pattern of Si(111) 7×7 to Si(111) 7×7-K. Subsequent heating of the Si(111) 7×7-K surface above 350℃ results in a Si(111) 7×7 with desorbing K. The RHEED pattern of the K-adsorbed on the Si(111) 7×7 surface up to the adsorption temperature of 250℃ was the modified 7×7 pattern, quite similar to that of the Si(111) 7×7-K, observed at room temperature. The 3×1 structure was observed in the temperature of adsorption between 300℃ and 550℃. Regardless of the adsorption temperature, a phase transition always took place from the Si(111) 3×1-K structure to Si(111) 1×1-K after annealing at 450℃ over 1 minute.