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Tanshinone IIA Reverses the Malignant Phenotype of SGC7901 Gastric Cancer Cells
Xu, Min,Cao, Fa-Le,Li, Nai-Yi,Liu, Yong-Qiang,Li, Yan-Peng,Lv, Chun-Lei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Backgrounds: Tanshinone IIA (TIIA), a phenanthrenequinone derivative extracted from Salvia miltiorrhiza BUNGE, has been reported to be a natural anti-cancer agent in a variety of tumor cells. However, the effect of TIIA on gastric cancer cells remains unknown. In the present study, we investigated the influence of TIIA on the malignant phenotype of SGC7901 gastric cancer cells. Methods: Cells cultured in vitro were treated with TIIA (0, 1, 5, $10{\mu}g/ml$) and after incubation for different periods, cell proliferation was measured by MTT method and cell apoptosis and cell cycling were assessed by flow cytometry (FCM). The sensitivity of SGC7901 gastric cancer cells to anticancer chemotherapy was investigated with the MTT method, while cell migration and invasion were examined by wound-healing and transwell assays, respectively. Results: TIIA (1, 5, $10{\mu}g/ml$) exerted powerful inhibitory effects on cell proliferation (P < 0.05, and P < 0.01), and this effect was time- and dose-dependent. FCM results showed that TIIA induced apoptosis of SGC7901 cells, reduced the number of cells in S phase and increased those in G0/G1 phase. TIIA also significantly increased the sensitivity of SGC7901 gastric cancer cells to ADR and Fu. Moreover, wound-healing and transwell assays showed that TIIA markedly decreased migratory and invasive abilities of SGC7901 cells. Conclusions: TIIA can reverse the malignant phenotype of SGC7901 gastric cancer cells, indicating that it may be a promising therapeutic agent.
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Liu, Xiaolu,Yan, Hai,Lv, Le,Xu, Qianqian,Yin, Chunhua,Zhang, Keyi,Wang, Pei,Hu, Jiye Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.5
A feeding trial was conducted to investigate effects of Bacillus licheniformis on growth performance and meat quality of broilers. Nine hundred one-d-old broiler chicks were randomly assigned to 3 experimental groups with three replicate pens of 100 broiler chicks. Three treatments were i) control, ii) basal diets supplemented with 1 ml of B. licheniformis for each in feed water per day iii) basal diets supplemented with 2 ml of B. licheniformis per chick in feed water per day. The supplementation of B. licheniformis significantly increased body weight in grower chickens (p<0.05), and significantly improved the feed conversion in 3 to 6 and 0 to 6 wk feeding period compared with the control group (p<0.05). Additionally, the supplement also resulted in increased protein and free amino acid contents, and decreased fat content in chicken breast fillet (p<0.05). Furthermore, improvement in sensory attributes was observed in broilers fed with the probiotic. In conclusion, B. licheniformis treatments resulted in a significant increase (p<0.05) in broiler productivity based on an index taking into account daily weight gain and feed conversion rate. Meanwhile, the probiotic contributed towards an improvement of the chemical, nutritional and sensorial characteristics of breast fillet. Overall, the study indicates that B. licheniformis can be used as a growth promoter and meat quality enhancer in broiler poultry.
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Yuze Xue,Mingwei Zhang,Le Xin,Luchao Ren,Panpan Lv,Hang Zhan,Jing He,Jiwei Zhai 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2
0.2ZnAl2O4/0.8Ba0.5Sr0.5TiO3 ceramics with good dielectric temperature stability were synthesized by controlling sinteringbehavior. The relationship between sintering conditions, microstructure, and dielectric properties of ceramics was studied. Cubic structures were confirmed in all ceramics. Double sintering (DS) behavior can effectively improve the density, and thegrain size has no obvious change compared with non-repeated sintering. The maximum dielectric permittivity (220 at 10 kHz)and minimum leakage current (3.98×10-6 A/cm2 at 0.25 kV/cm) are obtained in the DS ceramics, which is superior to thoseof the single sintering ceramics. This can be ascribed to the higher relative density of the DS ceramics in contrast to the singlesintering ceramics. More importantly, the temperature stability of the DS sample in all samples is optimal due to the highsurface energy at grain boundaries and improved density. This work demonstrates a route to produce ceramics with weaktemperature sensitivity for microwave applications.
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Economic Production of Probiotics from Kitchen Waste
Chunhua Yin,Xue Dong,Le Lv,Zhi-Guo Wang,Qianqian Xu,Xiaolu Liu,Hai Yan 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
In the present work, a novel treatment method of kitchen waste for economic production of probiotics was investigated. This required the selection of suitable probiotic microorganisms. Based on the pure cultures of probiotic strains for the fermentation kitchen waste, 5 strains of microorganisms including 1 strain of Lactobacillus, 2strains of Bacillus, and 2 strains of yeast were selected,respectively. These probiotic microorganisms were mixed at the same ratio and cultured using the kitchen waste as culture medium at pH of 7.2 and temperature of 37oC. After 24 h, the total count of the viable cells reached 2.24×1010 CFU/g, which was higher than that obtained in any single probiotic strain pure culture. It was found that the presence of yeasts and Bacillus species enhanced the growth of Lactobacillus strain. Bench scale experiments were also done in a self-designed rotating drum type bioreactor. The experimental results indicate that there is a good possibility of utilizing kitchen waste for the economic production of probiotics.
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Prevalence and Genotype Distribution of Human Papillomavirus among Women from Henan, China
Wang, Xiao-Chuan,Sun, Liang-Qi,Ma, Li,Li, Hua-Xin,Wang, Xiu-Li,Wang, Xin,Yun, Tian,Meng, Nian-Long,Lv, Da-Le Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Human papillomavirus (HPV) infection has been implicated as a causative of cervical cancer. In the present study, a total of 578 samples from females attending the gynecological outpatient clinic in Henan province, China, were collected and the HPV genotypes were detected by gene chip and flow-through hybridization. Overall, 44.5% (257/578) females were found to be HPV DNA positive, and the high risk HPV (HR-HPV) rate was 35.1% (203/578). The first peak of HR-HPV infection appeared in the >60 year-old group (55.0%), and the second was within the 51-55 year-old group (50.0%) (${\chi}^2$=19.497, p<0.05). HPV 16 was the most prevalent genotype (9.2%), followed by HPV 52 (7.8%), HPV 6 (6.9%), HPV 11 (5.9%) and HPV 42 (5.0%). The single type HPV infection was 30.4%, with the five majority prevalent genotype HPV 16 (16.5%), HPV 52 (14.3%), HPV 6 (12.6%), HPV 42 (8.6%), HPV 31 (5.1%). The multiple-type HPV infections were 14.0%, and HPV 16 was the most prevalent type (29.6%), followed by HPV 52 (24.7%), HPV 6 (22.2%), HPV 11 (22.2%), HPV 42 (17.3%) and HPV 39 (17.3%).
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