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- 저자 : Laila, Rawnak (검색결과 103 건)
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Laila, Rawnak,Robin, Arif Hasan Khan,Yang, Kiwoung,Choi, Gyung Ja,Park, Jong-In,Nou, Ill-Sup MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.1
<P>Clubroot is a soil-borne disease caused by the protist <I>Plasmodiophora brassicae</I> (<I>P. brassicae</I>). It is one of the most economically important diseases of <I>Brassica rapa</I> and other cruciferous crops as it can cause remarkable yield reductions. Understanding <I>P. brassicae</I> genetics, and developing efficient molecular markers, is essential for effective detection of harmful races of this pathogen. Samples from 11 Korean field populations of <I>P. brassicae</I> (geographic isolates), collected from nine different locations in South Korea, were used in this study. Genomic DNA was extracted from the clubroot-infected samples to sequence the ribosomal DNA. Primers and probes for <I>P. brassicae</I> were designed using a ribosomal DNA gene sequence from a Japanese strain available in GenBank (accession number AB526843; isolate NGY). The nuclear ribosomal DNA (rDNA) sequence of <I>P. brassicae</I>, comprising 6932 base pairs (bp), was cloned and sequenced and found to include the small subunits (SSUs) and a large subunit (LSU), internal transcribed spacers (ITS1 and ITS2), and a 5.8s. Sequence variation was observed in both the SSU and LSU. Four markers showed useful differences in high-resolution melting analysis to identify nucleotide polymorphisms including single- nucleotide polymorphisms (SNPs), oligonucleotide polymorphisms, and insertions/deletions (InDels). A combination of three markers was able to distinguish the geographical isolates into two groups.</P>
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Analysis of Billet Rolling in a Continuous Mill using Idle Vertical Stands
Laila S.Bayoumi,Youngseog Lee 대한기계학회 2004 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.18 No.5
An analytical approach is presented to investigate the deformation characteristics of billets in a continuous billet mill using power driven horizontal stands and idle vertical stands. The analysis is validated by comparison to the experimental results in a previously published work. The analytical results have shown that, apart from the problems of slip and buckling of billet, there are some shortcomings involved in this method. Compared to conventional rolling with all driven stands, the roll load for idle vertcal stands and the rolling torque for horizontal stands are almost doubled. The billet is severely stressed within the roll-bite of idle vertical stands and the overall rolling power has increased by one third of that for conventional roling. These shortcomings impair the feasibility of industrial application of idle vertical stand rolling method.<br/>
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Gene diversity shapes biological features of entomopathogenic Beauveria bassiana
Laila Gasmi,Sehyeon Baek,Jong Cheol Kim,Mi Rong Lee,Sihyeon Kim,So Eun Park,Dongwei Li,Tae Young Shin,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Beauveria bassiana is widely studied for its potential as biopesticide. However, little is known about the factors to influence genetic diversity among isolates. In this work, we aimed to study the gene diversity of 42 isolates to figure out the impact of sequences variability on various biological features. The accumulated data showed no correlation between sequence variability and the fungal geographic localization. Meanwhile, weak to strong correlations have been obtained between the sequence diversity of various genes and the studied biological features. Interestingly, Biotrophy-associated gene 2 gene variability was correlated with all the studied biological features, being an important marker to determine isolates of interest for biological control. Therefore, the obtained data would serve as a database to focus on interesting genes related to improving or screening highly virulent isolates as biological control agents.
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Laila Khaleda,차준영,김민갑,김외연 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
Arabidopsis GIGANTEA (GI) is encoded by asingle gene and highly conserved among vascular plants andits mutants display pleiotropic phenotypes involved indiverse biological processes such as light signaling, circadianclock, and sucrose metabolism as well as abiotic stressresponses. However, molecular mechanisms of GI arelargely unknown due to the lack of useful antibody. To date,the epitope tags have been widely used to detect GI in plants,but it needs to generate the transgenic plants which take afew months. Here, we produced polyclonal α-GI antibodyusing truncated variants of GI having amino-terminal (1-858aa) and carboxyl-terminal (920-1173) regions as antigens. Both recombinant His-GI1-858 and His-GI920-1173 proteins wereindividually and successfully expressed in E. coli andimmunized into rabbit. Anti-serum was purified by antigenspecificaffinity purification method using both recombinantHis-GI1-858 and His-GI920-1173 proteins. Purified polyclonal α-GI antibody not only detected endogenous GI proteins inwild-type Arabidopsis plants, but also reenacted its dieloscillations. Furthermore, the antibody showed cross-reactivitywith the GI orthologs in other plants such as Chinesecabbage, rape and tomato. Our polyclonal GI antibody couldhelp to determine the molecular mechanisms of GI involvedin largely unknown pleiotropic responses in plants.
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Laila Nuranna,Nyoman Bagus Donny,Gatot Purwoto,Hariyono Winarto,Tofan Widya Utami,Tricia Dewi Anggraeni,Alexander A.W. Peters 대한암예방학회 2017 Journal of cancer prevention Vol.22 No.2
Background: Cervical cancer is still the second most frequent cancer among Indonesian women, thus screening program is still critically important to prevent it. Visual inspection with acetic acid (VIA) was introduced as a method which is most suitable with Indonesia’s condition compared with the other screening methods. The Female Cancer Program from Jakarta Regional collaborated with Leiden University in 2007 to 2011 has done cervical cancer screening using VIA method, involving 25,406 women spreading across several primary health centers in Jakarta. By using these data, we found out the prevalence, age distribution, and risk factor of VIA positive in Jakarta as a basis to predict the budget and logistics for the next cervical cancer screening and to do an advocating to the Jakarta’s government. Methods: A secondary data analysis was conducted from several areas in Jakarta from 2007 to 2011. VIA test was used as the screening method, and performed by doctors and midwives with technical supervision by gynecologists. Results: From 25,406 women, there were 1,192 cases (4.7%) of VIA test positive. The risk factors that can significantly influence the result of VIA positive were number of marriage, parity, smoking habits, and the use of hormonal contraception with OR 1.51, 1.85, 1.95, and 0.68, respectively. Conclusions: Prevalence of VIA test-positive is 4.7% in Jakarta population. The findings of precancerous lesions and cervical cancers are not only between thirty and fifty years old, but also below the thirty years old and after fifty years old. We suggest that VIA test should be performed to all reproductive age and elder women who are not screened yet.
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( Laila Manni ),( Asmae Misbah ),( Nouhaila Zouine ),( Samir Ananou ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60℃. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H<sub>2</sub>O<sub>2</sub>, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40℃). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.
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Endotoxins of Enteric Pathogens Modulate the Functions of Human Neutrophils and Lymphocytes
( Laila N. Islam ),( A. H. M. Nurun Nabi ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.6
The locomotor responses of human peripheral blood neutrophils and lymphocytes were measured by the change from spherical to polarized shapes in the presence of endotoxins (lipopolysaccharide, LPS) of enteric pathogens: S. dysenteriue type 1, V.cholerae Inaba 569B, S. typhimurium, and K. pneumoniae. We reported earlier that these endotoxins are chemotactic factors for the neutrophils since they stimulated cell polarization within a few minutes of incubation. Endotoxins had an inhibitory effect upon neutrophil phagocytosis of opsonized yeast and the cells engulfed fewer yeasts. Interestingly, endotoxins increased neutrophil adhesion to clean glass surfaces, but stimulated the cells to exhibit increased random locomotion (chemokinesis) through cellulose nitrate filters and show an enhanced ability to reduce nitroblue tetrazolium (NBT) dye. Unlike neutrophils, lymphocytes direct from blood do not show polarized morphology towards chemotactic factors but the cells acquire locomotor capacity during 24-72 h culture with mitogens uch as phytohemagglutinin (PHA), phorbol myristate acetate or concanavalin A. Stimulation of blood lymphocytes with endotoxins did not induce cell polarization in short-term but long-term culture resulted in an increase in the proportion of polarized cells that acquired locomotor morphologies. The majority of these cells were identified as esterase negative B-lymphocytes that migrated through filters. Despite the optimum time of incubation for each of these cell types being different, we found that lymphocytes respond to much lower concentrations of endotoxins than the neutrophils. These findings suggest that endotoxins of enteric pathogens modulate the functions of human blood neutrophils and lymphocytes.
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Laila Arjuman Ara 한국외국어대학교 인도연구소 2014 남아시아연구 Vol.20 No.1
Various ASEAN member states including Singapore, Vietnam, and Thailand are negotiating bilateral Free Trade Agreement(FTAs) with the EU. Recently, Myanmar has received EBA from the EU. Most of the ASEAN countries are main competitors of Bangladesh in the EU market. The main objective of the study is to investigate the potential economic impacts on Bangladesh of the proposed EU?ASEAN free trade agreement. In this context, a Computable General Equilibrium(CGE) analysis has been developed by using the Global Trade Analysis Project(GTAP) model and database to explore the aggregate impact of the proposed FTA. The CGE analysis evinces that if EUASEAN signs FTA, Bangladesh would experience a fall in real GDP of 0.13 per cent and welfare losses would be USD 22 million. Bangladesh’s export could also fall by about 0.16 percent to the EU market. This study also shows that if the EU and Vietnam sign an FTA, Bangladesh’s exports to the EU are expected to drop by about 0.04 per cent and the welfare loss equals USD 5 million. Bangladesh would experience a fall in real GDP by 0.03 per due to sharp deterioration of terms of trade. This suggests that if EU?ASEAN signs an FTA, Bangladesh will face formidable market access difficulties in the EU.
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