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RISS 인기검색어
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- 저자 : L. Zhong Liang (검색결과 2 건)
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Characteristics of complicated AISI316L automobile components manufactured by powder/metallurgy
L. Zhong Liang,L. Jin Hui,S. Yu Sheng,Y. Chun Ze 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.7
Indirect Selective Laser Sintering/ Isostatic Pressing (SLS/IP), with Cold Isostatic Pressing (CIPing) and Hot Isostatic Pressing (HIPing) IPs, is adopted for the manufacture of complicated automobile components. The preparation of PA12-coated AISI316L powder and airproof plastic canning during CIPing are also investigated. The influence of technology parameters on the performances of AISI316L specimens during SLS/IP is likewise analyzed. Results show that PA12-coated AISI316L powders are successfully prepared through the dissolution and precipitation process, and that it is better to fabricate airproof canning for complicated components with natural latex. After sintering AISI316L specimens from 1300℃ to 1340℃ (CIPed at more than 300 MPa ahead), their relative densities increased, approaching 92%. Subsequently, their relative densities are improved by HIPing, whose optimal pressure and temperature parameters are between 90 MPa and 20 MPa, and 1150℃ and 1250℃, respectively. The optimal tensile performances are close to those of annealed AISI316L compact materials.
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Liang, Zizhen,Zhu, Ning,Mai, Keith K.,Liu, Zhongyuna,Tzeng, David,Osteryoung, Katherine W.,Zhong, Silin,Staehelin, L. Andrew,Kang, Byung-Ho American Society of Plant Biologists 2018 The Plant cell Vol.30 No.7
<P>Electron tomography, immunoblot, immunolabeling, and RNA-seq analyses of thylakoid assembly in germinating Arabidopsis revealed five structural assembly stages and the sequential incorporation of photosystem II, cytochrome <I>b<SUB>6</SUB>f</I>, ATP synthase, light-harvesting complex II, photosystem I, and CURT1A subunits within 84 h after imbibition.</P><P>Biogenesis of the complex 3D architecture of plant thylakoids remains an unsolved problem. Here, we analyzed this process in chloroplasts of germinating <I>Arabidopsis thaliana</I> cotyledons using 3D electron microscopy and gene expression analyses of chloroplast proteins. Our study identified a linear developmental sequence with five assembly stages: tubulo-vesicular prothylakoids (24 h after imbibition [HAI]), sheet-like pregranal thylakoids that develop from the prothylakoids (36 HAI), proliferation of pro-grana stacks with wide tubular connections to the originating pregrana thylakoids (60 HAI), structural differentiation of pro-grana stacks and expanded stroma thylakoids (84 HAI), and conversion of the pro-grana stacks into mature grana stacks (120 HAI). Development of the planar pregranal thylakoids and the pro-grana membrane stacks coincides with the appearance of thylakoid-bound polysomes and photosystem II complex subunits at 36 HAI. ATP synthase, cytochrome <I>b<SUB>6</SUB>f</I>, and light-harvesting complex II proteins are detected at 60 HAI, while PSI proteins and the curvature-inducing CURT1A protein appear at 84 HAI. If stromal ribosome biogenesis is delayed, prothylakoids accumulate until stromal ribosomes are produced, and grana-forming thylakoids develop after polysomes bind to the thylakoid membranes. In <I>fzo-like</I> (<I>fzl</I>) mutants, in which thylakoid organization is perturbed, pro-grana stacks in cotyledons form discrete, spiral membrane compartments instead of organelle-wide membrane networks, suggesting that FZL is involved in fusing membrane compartments together. Our data demonstrate that the assembly of thylakoid protein complexes, CURT1 proteins, and FZL proteins mediate distinct and critical steps in thylakoid biogenesis.</P>
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