Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Cho, Kyu-Hyuk,Cho, Jae-Woo,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 mg/kg ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in $G_1,\;G_2\;and\;G_3$ mice. One hundred thirty five $G_1$ phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen $G_3$ recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Kyu-Hyuk Cho,Jae-Woo Cho,Chang-Woo Song 한국독성학회 2008 Toxicological Research Vol.24 No.1

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 ㎎/㎏ ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in G₁, G₂and G₃mice. One hundred thirty five G₁phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen G₃recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

소양호 동물성플랑크톤의 수직분포

조규송,박영춘,조성주 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

본 연구는 소양호의 동물성 플랑크톤의 군집조사를 파악하기 위해 플랑크톤의 수직분포를 알아보았다. 조사기간은 1986년 3월부터 9월까지 실시하였다. 본 호수의 동물성 플랑크톤은 담수 초기에 출현된 종이 그대로 장착되고 있으나 식물성 플랑크톤은 종 및 밀도가 증가햐였으며 이는 수여고한경의 변화를 시사해 주고 있다. 개체군 밀도가 가장 높은 시기는 5월과 6월로 ㎥당 개체수가 40,000개에 달하고 가장 적은 시기는 3, 4월로 약 5,000개 정도 였다. 동물성 플랑크톤의 수직분포에서 정체기와 순화기때 서로 다른 양상을 보이는 종들로는 Keratella cochlearis, Therrnocycolps

황성 섬강 상류의 어류상

송호복,권오길,전상호,김휘중,조규송 江原大學校 附設 環境硏究所 1996 環境硏究 Vol.13 No.-

Fish fauna was investigated from June to November 1993 at the upper Sum river in Hoengsong. Form of stream was Aa and Aa-Bb, and pH, DO and BOD were 7.3~7.9, 8. 8~10.2mg/1 and 1.3~l. 5mg/1. The collected fishes were classified twenty nine species belonging to twenty two genera and seven family , of witch twelve species were endemic species of Korea. Dominant species was Zacco temmincki and subdominant species was Coreoleuciscus splendus. Zacco platypus, Microphgsogobio longidorsalis and Acheilognathus signifer were also numerous. Scarce species were Hemibarbus labeo, Coditis lutheri and the like. The species diversity index, dominance index and evenness index were 2.552, 0.384 and 0.758.

중·고등학교 가정과 「의복마련계획」 영역의 의복 색채 선택 학습을 위한 지도 방안

조성희,강경원,송선희,정규욱 전주대학교 교육문제연구소 2000 敎育論叢 Vol.15 No.-

永郞湖의 基礎生産에 關하여 (豫報)

曺圭松 春川敎育大學 1968 論文集 Vol.0502 No.-

This preliminary survey investigated lake Yong-rang on the east coast of Kangwoondo province, Korea, for the purpoae of studying primary productivity of the lake. The field studies were carried out in the summer of 1966, and dissolved oxygen was measured by Winkler's method. From the standpoint of primary productivity, the result of this survey indicated that the lake is mesotrophic type, and the lake being brackish water is often influenced on its metabolism by sea water flowing into it. Therefore, it is believed that the tide becomes the limiting factor of primary production.

大巖山 高層濕原의 枝角類

조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Zooplankton community was investigated in a small bog lake located in mountainous district of middle part of Korea. Four species of Cladocera were identified; Daphnia pulex, Daphnia ambigua, Sinocephalus serruiatus, erriodaphnia rigaudi. And two of them, Daphnia ambigua, Sinocephalus serrulatys, were unreported species in Korea. The zooplankton community composed of mixture of southern and northern species. The dual geature of southern and northern trait implys this community is biogeographically in transient status.

金剛山댐 建設에 따른 北漢江 上流 水域의 環境 모니터링에 관한 硏究

조규송,김범철,안태석,전상호,이종범 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Aquatic ecosystem was studies in Lake Paro to monitor the effects of the Keumkangsan Dam construction in North Korea. Water quality, primary prodectivity, and microbes were serveyed bimonthly in 1987. The texture and composition of sediments, and meterological sapects were also investigated. The most remarkable change of water quality was the increase of turbidity. due to the dam construction. Overall chemical and biological aspects were greatly changed because of the high turbidity. The concentration of phosphate increased and subsequently phytoplankto standing corp increased. The effect of phosphate increase was compensated by the decrease of light penetration, and, therefore areal primary prodectivity was not so high as expected from the chlorophyll concentration. From the standing corp of microbes, the trophic state of lake Paro was evaluated as mesotrophy. As for the horizontal variation of microbial activity, the microbial heterotrophy was higher in the upstream regions than in the downstream regions. Sediment types are almost clayey silt and the mean sizes of sediments were homogeneous through the whole lake area. Organic carbon contents of the sediment seemed to be influenced by seasonal variation of primary productivity.

상 요추 추간판 탈출증의 수술적 치료

조태현,송준혁,김명현,박향권,김성학,신규만,박동빈 梨花女子大學校 醫科大學 醫科學硏究所 2000 EMJ (Ewha medical journal) Vol.23 No.3

Objectives : Lumbar disc herniations at the L1-L2, L2-L3, and L3-L4 level are rare. The purpose of this study is to observe the variable clinical finding of upper lumbar disc herniations and to provide proper treatment modality. Patients and Methods : Between 1998 Jan. and 1999 Dec., seventeen patients with upper lumbar disc herniations who were undergone surgery in our institute were retrospectively evaluated. Patients were evaluated based on following factors : age, sex, aggravating factor, symptoms and signs type of disc herniation, type of surgery, and outcome of post-surgery. Results : The incidence of lumbar disc herniation at the level of L1-2, L2-3 and L3-4 is 6.1% with declining frequency as the level ascends. The peak age incidence is 6th decade in both sex. Preoperative symptomes and signs are variabe. In sixteen cases, posterior approach was done. In these cases, twelve cases were performed microscopic partial hemilaminectomy, and four cases with spinal stenosis were performed total laminectomy and posterior screw fixation with bone fusion. In one case, lateral extracavitary approach was done. In results of operation, fourteen cases showed more than good grade(82%). Conclusion : The age incidence of lumbar disc herniations at the level of L1-2, L2-3 and L3-4 older than lumbar disc herniations at the lower level of L4-5 and L5-S1. The signs and symptoms are variable. In our cases, most of the patients were performed posterior approach with microscopic partial hemilaminectomy except the patients combined spinal stenosis. One case was performed a lateral extracavitary approach because the risk of the cord and cauda equina injuries. The prognosis of upper lumbar disc herniations after treatment with only microscopic partial hemilaminectomy and diskectomy is comparable with the prognosis of lumbar disc herniations at the lower level.

Cluster analysis 에 의한 昭陽湖의 水體 分布

조규송,안태석,김범철,이동훈 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Cluster analysis was applied for the distinction of the water body in Soyang lake, which is the largest lake in Korea. For this analysis, the sixteen environmental parameters, such as water temperature, nutirent salts, organic materials, ets were used as the input data. The total data matrix was 16parameters X 47 points (in 14 sites). After Standardization, the Euclidean distances between two points were calculate. By the results, main stream of Soyang lake can be classified with 5 groups in March, 10 groups in February. And two shallow tributaries, showed different grouping pattern from main stream. In March, and November, the vertical mixing was detected, and in August, the water body above 10m was showed complex grouping pattern, and showed two water bodies in middle layer, and one in bottom layer.