섬유보강 다공성 옥상녹화 황토콘크리트의 물리·역학적 및 온도변화 특성 평가

오리온,김춘수,김황희,전지홍,권완식,박찬기,Oh, Ri On,Kim, Chun Soo,Kim, Hwang Hee,Jeon, Ji Hong,Kwon, Wan Sig,Park, Chan Gi 한국농공학회 2013 한국농공학회논문집 Vol.55 No.4

The physical, mechanical, water purification and temperature properties of fiber reinforced porous hwang-toh green roof concrete have been evaluated in this study. The effect of the depending on replacement ratio of blast furnace slag to cement was investigated such that the replacement ratio is varied to 0 % and 30 %. Also, the replacement ratios of hwang-toh were 0, 20 and 30 %. The polyvinyl alcohol fiber was used for the reinforcing fiber. A series of pH test, unit weight, void ratio, compressive strength, after purification and variation of temperature test have been performed to evaluate the performance, water purification effect and temperature properties of the fiber reinforced porous hwang-toh green roof concrete. The test results indicate that the physical and mechanical properties of fiber reinforced porous hwang-toh green roof concrete is affected by the replacement ratio of the blast furnace slag and hwang-toh contents. Results of purifying water showed that the water purification effect of porous hwang-toh green roof concrete is about 40 %. Also, the temperature properties test results indicate the green roof blocks using fiber reinforced porous hwang-toh green roof concrete have insulation and temperature reduction effect.

디메틸 실록산과 2-히드록시에틸메타크릴레이트계 블락공중합체의 합성 : 초임계 이산화탄소용 양손잡이형 계면활성제 Ambidextrous Surfactant for Supercritical Carbon Dioxide

황하수,이민영,임권택 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

Block copolymers containing dimethy siloxane and 2-hydroxyethyl methacrylate sequences were synthesized by group transfer polymerization (GTP) of 2- trimethylsilyloxy ethyl methacrylate(TMS-EMA) using silyl ketene acetal terminated poly(dimethylsiloxane) (PDMS) as macroinitiator, followed by hydrolysis of TMS-EMA to HEMA. The block copolymers were obtained with controlled molecular weight and narrow molecular weight distribution. Trimethylsilyl groups in the P(TMS-EMA) block could be selectively hydrolyzed without interfering with Si-O bond in PDMS block. The block copolymers formed micelles in methanol, the effective diameters (Rh) of which were in the range of 78 ~110 nm with narrow distribution by dynamic light scattering (DLS). The latex particles, PMMA was synthesized by dispersion polymerization in supercritical carbon dioxide with these block copolymers as stabilizer.

관동맥질환에서 각종 지질 및 지단백의 변화

황석순,김권배,손수인,서영숙,김기식,김윤년 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3

To evaluate the relationship between coronary artery disease(CAD) and the various levels of lipid and lipoproteins, we performed coronary angiography to the patient, who were suspected coronary artery disease from January 1988 to August 1989. And compared it with normal control group. The number of control group were 41(21 men, 20 women), and the patient group were 52(42 men, 10 women). The age was not differ between two groups. The results were as follows; 1. In CAD group, the plasma concentration of total lipid, triglyceride, total cholesterol, phosphlipid, and TC/HDL ratio were significantly higher than in control group. But, though, the concentration of HDL was lower in CAD group, had no significant difference between two groups. 2. The severity of CAD were well correlated with total lipid, cholesterol, LDL, trigylceride, phospholipid, TC/HDL, LDL/HDL levels. HDL level didnt's show significant correlation with the degree of CAD. We concluded that total lipid, cholesterol, trigylceride TC/LDL ratio were valuable markers of CAD in Korean, but futhur studies of lipoproteiins and other risk factors in the large population will be recommended.

일부 흡연 대학생의 지각된 스트레스와 흡연실태에 대한 상관관계 연구

강초록,권시내,권혜림,박수경,송인혜,오해인,이미지,한승아,황선아,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

In spite of the seriousness of smoking harm has been developed these days. the increase of national smoking rate and that the people in the twenties are especially leading the rise of national smoking rate is an important issue of national health. Smoking of the university students can occur health problem and cause loss of manpower and expenses in their social activity period. So then the actual state for prevention of smoking and development of a program to prohibit smoking has to be seized urgently and starting with the study of the realities of university students, the effort to find a related fluent of smoking has to be studied. Related to this, the purpose of this study is to seize the general characteristics of smoking university students and to find out the relationship between perceived stress and smoking status among smoking university students. In addition, by collating the relationship between perceived stress and smoking status among smoking university students we have the purpose to furnish the basal data of stress care, the best way to cope with stress through group training and the plan for nursing implementation to decrease the smoking rate. For this study, a survey was conducted for 204 smoking university students of 13 universities in Seoul from 9th of May to 16th of May, 2003. The collected data was analyzed by t-test, Pearson Correlation and ANOVA in the statistical packages SPSS/PC. Major findings of this study are as follows; 1. Of the 204 respondents 85.4% of them were male and 14.6% of them were female. The average age of the sample was 23.19 years old and 41.8% of them were in senior, 21.4% in junior, 16.9% in sophomore, 19.9% in freshman grade. The major motivation to start smoking was 'because of curiosity'(33.8%) and the first smoking experience was at 'senior high school' (41.9%) and at 'college' (41.9%). 2 The highest scored stress factor of smoking university students were stresses about school work. 3. 72.7% had experience to quit smoking at least one or more times. Most of the reason to keep on smoking was 'short will to quit'(51.0%) and the smoking place was usually at 'school' (41.4%). Most respondents for the number ofsmoking friends were '5 people and above' (73.2%). 4. There was a negative relationship between the general characteristics and the entire perceived stress, however, there was a positive relationship between the general characteristics and the perceived stress in 4 categories which is 'sex distinction', 'major of study', 'residence form' and' inmate'. 5. There was a negative relationship between the general characteristics and the entire smoking status, however, there was a positive relationship between the general characteristics and the smoking status in 2 categories which is 'presence of hobby' and 'number of smoking friends'. 6. There was a negative relationship between the perceived stress and the smoking status. Through this study we have seized the relationship between the general characteristics, perceived stress and the smoking status of smoking university students. There was a positive relationship between some of the general characteristics with the perceived stress and the smoking status of smoking university students. Also there was a negative relationship between the perceived stress and the smoking status.

Generation of Transgenic Rice without Antibiotic Selection Marker through Agrobacterium-mediated Co-transformation System

Soo-Kwon Park(박수권),Tackmin Kwon(권택민),Jong-Hee Lee(이종희),DongJin Shin(신동진),Woon-Ha Hwang(황운하),You-Chun Song(송유천),Jun-Hyun Cho(조준현),Min-Hee Nam(남민희),Seung-Ho Jeon(전승호),Sang-Yeol Lee(이상열),Dong-Soo Park(박동 한국생명과학회 2012 생명과학회지 Vol.22 No.9

작물의 수확량이나 병 저항성을 증가시키는 형질전환 식물체 개발은 세계 식량 부족을 해결하는 좋은 방법이다. 하지만 항생제나 제초제의 사용은 형질전환 작물의 안전에 대해서 일반 사람들의 심각한 우려를 초래한다. 본 연구에서는, 아그로박테리움을 이용한 동시 형질전환 방법을 이용하여 한국의 밀 재배종인 ‘조경밀’의 유전자인, 고분자 글루테닌 서브유닛[high molecular-weight glutenin subunit (HMW-GS)] Dx5가 삽입된 마커프리 형질전환벼를 개발하였다. 각각 Dx5 유전자와 하이그로마이신(HPTII) 저항성 유전자만으로 구성된 두 종류의 발현 카셋트(Two expression cassettes)를 독립적으로 아그로박테리움 EHA105에 도입하였고, Dx5와 HPTII가 도입된 각각의 EHA105 아그로박테리움을 3:1 비율로 혼합하여 벼 캘러스에 접종하였다. 66개의 HPTII 저항성 형질전환체 중에서 벼 게놈에 Dx5와 HPTII가 모두 삽입된 2개의 형질전환 라인을 획득하였다. Dx5와 HPTII가 벼 게놈에 도입된 것을 Southern blot을 통해서 다시 확인하였다. 또한, semi-quantitative RT-PCR을 통해 형질전환벼 T1 세대 종자의 밀 Dx5 전사여부를 확인하였고 결국, Dx5 유전자만을 가지는 마커프리 형질전환벼를 T1 세대에서 선발할 수 있었다. 본 연구 결과는 두 종류의 발현 카셋트를 사용한 아그로박테리움 동시 접종 시스템이 마커프리 형질전환벼를 생산하기 위한 효과적인 전략이 될 수 있음을 보여준다. Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene (Dx5) from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the Dx5 and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with Dx5 gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the Dx5 and HPTII genes into the rice genome. We reconfirmed integration of the Dx5 and HPTII genes into the rice genome by Southern blot analysis. Wheat Dx5 transcripts in T1 rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the Dx5 gene were successfully screened at the T1 generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.

Genetic transformation of a high-molecular-weight glutenin Dx5 to Donjin rice cultivar

Soo-Kwon Park,Tackmin Kwon,Woon-Ha Hwang,Donjin Shin,Se Yun Oh,Seong-Hwan Oh,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

In order to improve rice dough functionality, we cloned 4 kinds of high-molecular-weight glutenin subunit (HMW-GS) genes from bread wheat, ‘Jokyeong’. Among them, we first examined Dx5 gene to generate marker-free transgenic rice for advanced quality processing of bread and noodles. The GluB1 promoter was inserted into binary vector for seed specific expression of the Dx5 gene. Two expression cassettes comprised of separate DNA fragments containing only the high-molecular-weight glutein subunit (HMW-GS) protein (Dx5) and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected to rice calli at 3: 1 ratio of Dx5 and HPTII, respectively. Then among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted both with Dx5 and HPTII gene to rice genome. We reconfirmed integration of the Dx5 and HPTII genes into the rice genome by Southern blot analysis. Wheat Dx5 transcriptsin rice seeds was examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only Dx5 gene were successfully screened at T1 generation. This result also provides that co-infection system with two expression cassettes could be efficient strategy to generate marker-free transgenic rice plants.

Fine mapping and identification of candidate rice genes associated with qSTV11 SG , a major QTL for rice stripe disease resistance

Kwon, Tackmin,Lee, Jong-Hee,Park, Soo-Kwon,Hwang, Un-Ha,Cho, Jun-Hyun,Kwak, Do-Yeon,Youn, Yeong-Nam,Yeo, Un-Sang,Song, You-Chun,Nam, Jaesung,Kang, Hang-Won,Nam, Min-Hee,Park, Dong-Soo Springer-Verlag 2012 TAG. Theoretical and applied genetics. Theoretisch Vol.125 No.5

Molecular re-confirmation and floral characteristics of drooping leaf (DL) mutants generated by insertional mutagenesis in rice

Soo-Kwon Park,권택민,Jong-Hee Lee,Gihwan Yi,Norvie L. Manigbas,Sang-Min Kim,You-Chun Song,Jun-Hyun Cho,Woon-Ha Hwang,Un-Sang Yeo,Hang-Won Kang,이승식,Kang-Seob Lee,Ung-Han Yoon,한창덕,정영준,이상열,Dong-Soo Park 한국유전학회 2011 Genes & Genomics Vol.33 No.5

Transposon tagging and insertional mutagenesis provide one of the most powerful tools in gene function studies. Here, we report a comparison between two novel drooping leaf (DL)mutants from transposon and T-DNA insertion lines of rice. DL is distinct from well-known ABC genes and a member of the YABBY gene family, and it is closely related to the CRABS CLAW (CRC) gene of Arabidopsis thaliana. Based on phenotypic analysis, DL regulated midrib formation by promoting cell proliferation in the central region of rice leaf and was necessary for the specification of carpel identity. We identified two DL mutants by screening the Ac/Ds and T-DNA insertional mutant pool of rice. Flanking sequence tag analysis indicated that both Ds and T-DNA segments were inserted in the promoter region at 3.4 kbs and 5.4 kb upstream, respectively,of the previously known OsYABBY domain. Interestingly, the progenies of DL lines of two different pools showed various degrees of leaf drooping and abnormal carpel formation. Flower structures revealed that there were more than two stigmas with normal stamens and pistils per panicle in the Ds-induced mutants. However, T-DNA induced mutant had extra stamens with staminoid carpels. These results indicate that the promoter region of DL plays an important function in regulating anther and carpel formation.

Development of Marker-free Transgenic Rice Expressing the Wheat Storage Protein, Glu-1Dy10, for Increasing Quality Processing of Bread and Noodles

Soo-Kwon Park(박수권),DongJin Shin(신동진),Woon-Ha Hwang(황운하),Yeon-Jae Hur(허연재),Tae-Heon Kim(김태헌),Se-Yun Oh(오세윤),Jun-Hyun Cho(조준현),Sang-Ik Han(한상익),Seung-Sik Lee(이승식),Min-Hee Nam(남민희),Dong-Soo Park(박동수) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

쌀가루는 많은 식품 가공에 이용된다. 그러나 밀가루 반죽이 빵과 면을 포함한 많은 식품 가공 제품에 적합한 반면에, 쌀로 만든 반죽은 신장성과 탄력성이 부족하다. 고분자 글루테닌 서브유닛(HMW-GS)은 밀의 가공 적성을 결정하는데 중요한 역할을 한다. 본 연구에서, 우리는 아그로박테리움(Agrobacterium) 동시 형질전환법을 이용하여 한국 밀 품종인 ‘조경’으로부터 HMW-GS를 암호화하는 밀 Glu-1Dy10 유전자를 발현하는 marker-free 형질전환 벼 식물체를 개발하였다. 오직 Glu-1Dy10 유전자와 HPTII (hygromycin phosphotransferase II) 저항성 유전자만을 포함하는 분리된 DNA 조각들로 구성된 두 가지 발현 카셋트(cassettes)를 독립적으로 아그로박테리움(Agrobacterium) EHA105 에 도입하였다. Glu-1Dy10 또는 HPTII를 함유하는 EHA105 를 각각 3:1 비율로 벼 캘러스에 접종하였다. 290개의 하이그로마이신(hygromycin) 저항성 T0 식물체 중에서 우리는 벼 게놈에 Glu-1Dy10과 HPTII 유전자가 모두 삽입된 29개의 형질전환 라인을 획득하였다. 우리는 Glu-1Dy10 유전자가 벼 게놈 내로 도입된 것을 Southern blot 분석을 통해 다시 확인하였다. 형질전환 벼 종자에서 Glu-1Dy10의 전사(Transcripts)와 단백질을 semi-quantitative RT-PCR과 Western blot 분석을 통해서 확인하였다. 최종적으로, 오직 Glu-1Dy10 유전자를 갖는 marker-free 식물체를 T1 세대에서 성공적으로 선발할 수 있었다. Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, making it less suitable than wheat for many food products such as bread and noodles. The high-molecular weight glutenin subunits (HMW-GS) of wheat play a crucial role in determining the processing properties of the wheat grain. This paper describes the development of marker-free transgenic rice plants expressing a wheat Glu-Dy10 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation. Two expression cassettes, consisting of separate DNA fragments containing Glu-1Dy10 and hygromycin phosphotransferase II (HPTII) resistance genes, were introduced separately into Agrobacterium tumefaciens EHA105 for co-infection. Each EHA105 strain harboring Glu-1Dy10 or HPTII was infected into rice calli at a 3: 1 ratio of Glu-1Bx7 and HPTII. Among 290 hygromycin-resistant T0 plants, we obtained 29 transgenic lines with both the Glu-1Dy10 and HPTII genes inserted into the rice genome. We reconfirmed the integration of the Glu-1Dy10 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the Glu-1Dy10 in transgenic rice seeds were examined by semi-quantitative RT-PCR and Western blot analysis. The marker-free plants containing only the Glu-1Dy10 gene were successfully screened in the T1 generation.

QTL mapping of agronomic traits using an RIL population derived from a cross between temperate <i>japonica </i>cultivars in rice (<i>Oryza sativa</i> L.)

Kwon, Soo-Jin,Cho, Young-Chan,Kwon, Soon-Wook,Oh, Chang-Sik,Suh, Jung-Pil,Shin, Young-Seop,Kim, Yeon-Gyu,Holligan, Dawn,Wessler, Susan R.,Hwang, Hung-Goo,Ahn, Sang-Nag Japanese Society of Breeding 2008 Breeding science Vol.58 No.3

<P>To employ molecular marker-aided selection for developing <I>japonica</I> rice (<I>Oryza sativa</I> L.) cultivars with high yielding potential and good eating quality, we carried out QTL analysis for agronomic and grain traits using 231 recombinant inbred lines (RIL) from a cross between two temperate <I>japonica</I> cultivars, Suweon365 and Chucheongbyeo. A linkage map with 221 loci, including 134 simple sequence repeats (SSRs), 66 amplified fragment length polymorphisms (AFLPs), and 21 miniature inverted repeat transposable elements (MITEs)-anchored markers, was constructed covering 2,227 cM of the twelve rice chromosomes, with an average distance of 11.0 cM between markers. The proportion of polymorphic bands between parents averaged 19.5%, 12.9% and 42.3% for SSRs, AFLPs and MITEs, respectively. Construction of maps without AFLP or MITE markers indicated that expansion of this map is due to the incorporation of AFLP markers, and this result is consistent with previous reports. Twenty-seven QTLs for ten traits were identified over two years, and fourteen were consistently detected in two consecutive years. When the locations of each QTL detected in this study were compared with those in previous QTLs studies, eight QTLs for culm length, spikelets per panicle and, thickness, length and shape of grain, had not been reported in previous mapping studies, indicating potentially novel alleles of <I>japonica</I> rice. Interestingly, a cluster of seven QTLs, including heading date and grain morphology traits, was detected on the long arm of chromosome 7. The markers linked to these QTLs would be valuable in a marker-assisted breeding program for <I>japonica</I> cultivars.</P>