콩나물 생장중 세포벽 비섬유성 중성당의 변화

신승렬,김광수,김주남 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

본 연구는 콩나물의 물성에 미치는 콩나물 성장시 세포벽 다당류를 구성하고 있는 비섬유성 중성당의 변화를 조사하였다. 자엽부 세포벽의 중성당 조성은 rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose 등이었고 주요 중성당은 galactose, arabinose, xylose 이었다. 배축부의 구성 중성당의 조성은 자엽부와 동일하였고, 성장함에 따라 대부분의 당이 감소하였고, 그 중에서도 galactose는 성장에 따라 현저히 감소하였으며, 성장 4일과 6일에는 오히려 galactose보다 arabinose 함량이 높았다. 배축부의 총 비섬유성 중성당 함량은 성장함에 따라 감소하였다. Pectin질의 중성당은 rhamnose가 CBP보다 IAP에서 많았고, galactose는 CBP에 많았으며, 성장함에 따라 IAP와 CBP의 arabinose는 자엽부와 배축부에서 다같이 증가하였다. Hemicellulose의 주용 중성당은 자엽부에서는 glucose, rhnmnose, galactose,xylose이었고 알칼리가용성 hemicellulose의 rhamnose 함량은 산가용성 hemicellulose에 비해 낮았으며, 성장중 galactose의 함량이 현저히 감소하였다. This study was carried out to changes and composition of the non-cellulosic neutral sugars in soybean sprouts during growth. The composition of non-cellulosic neutral sugars in cell wall of soybean sprouts were rhamnose, fucose, xylose, arabinose, mannose, galactose and glucose. The galactose contents of cell wall were higher than other non-cellulosic neutral sugars, and was remarkly decreased during growth. The major non-cellulosic sugars of pectic substances were rhamnose, arabinose, and galactose. The arabinose contents of pectic substance were increased in cotyledon and hypocotyl during growth. The contents of non-cellulosic neutral sugars were in hypocotyl during growth. The galactose contents of pectic substance were higher in cotyledon than those in hypocotyl associated pectic substances than those in covalently bounded pectic substances, arabinose and galactose. The galactose of hemicellulose was decreased remarkably during growth.

과실의 연화중 세포벽 다당류와 관련효소의 변화

신승렬,김광수 대구효성가톨릭대학교 식품과학연구소 1993 식품과학지 Vol.5 No.-

New information of softening related changes in cell wall structure, cell wall polysaccharides, and its degradation enzymes were summarized from 120 references. Changes in the activities of polygalacturonase, β-galactosidase, cellulase, and pectinmethylesterase during softening of fruits were discussed. And also, softening related changes in cell wall structure, sugars components and neutral sugars were discussed.

Adamkiewicz 동맥의 폐색에 의한 척수경색 1례

이광훈,이종걸,이태연,신동익,이성현,이상수,한설희,차상훈 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

만성신부전 환자에서 Recombinant Human Erythropoietin(rhEPO)의 피하주사시 생체이용율

김경수,이경훈,이영수,김광호,김용식,신상구,박찬웅 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

Imipramine과 Reserpine이 신경말단의 Ca^++이동에 미치는 효과

신정인,이정수,이광수 중앙대학교 의과대학 의과학연구소 1986 中央醫大誌 Vol.11 No.1

Since calcium ion is known to be associated in the initiation of stimulus-induced neurotransmitter release from nerve endings, effects of imipramine and reserpine on the Na^+-K^+ ATPase, Mg^++ ATPase, Ca^++-Mg^++ ATPase and Na^+-Ca^++ ATPase activities in rat brain synaptosomes which may be associated with adrenoceptor were investigated. In view of the possible role of mitochondria in the control of intracellular Ca^++ concentration, suggested effects of imipramine and reserpine on the mitochondrial Ca^++ uptake and the Na^+-induced Ca^++ release from mitochondria were also studied. Na^+-K^+ ATPase activities in rat brain synaptosomes were inhibited by imipramine in a dose dependent manner. Reserpine, however showed no effect on or slightly increased these ATPase activities. Na^+-K^+ ATPase. Mg^++ ATPase and Ca^++-Mg^++ ATPase activities were all enhanced by norepinephrine and dopamine, but Na^+-Ca^++ ATPase activity inhibited by these agents. The effects of these catecholamines on these ATPase activities were antagonized by propranolol or yohimbine. Imipramine inhibited all the above mentioned ATPase activities in the same degree in the presence or absence of the catecholamines. Reserpine decreased the effects of catecholamines on these ATPase activities. The initial rate of calcium binding to mitochondria was enhanced by imipramine, but the maximum calcium binding was not affected. In the presence of reserpine or verapamil, both the maximum calcium binding and the initial binding rate were inhibited. With regard to Na^+-induced Ca^++ release from mitochondria, both the amount and rate of calcium release were enhanced by imipramine, but were not altered by reserpine. The above findings suggest that imipramine may increase the free intracellular calcium concentration through the inhibition of Na^+-K^+ ATPase, Mg^++ ATPase, Ca^++-Mg^++ ATPase and Na^+-Ca^++ ATPase activities which may result in enhancement of initial binding and subsequent release of Ca^++ to and from mitochondria. On the other hand, reserpine may induce consistent release of neurotransmitters through the inhibition of the ATPase which may play a regulatory role in the release of neurotransmitters and it may maintain an increased intracellular free calcium by the inhibition of calcium binding to mitochondria.

B형 간염 동반 원발성 간암 환자에서의 CYP2D6와 NAT2 다형현상에 관한 연구

신동일,이광수,최연남,황기은,조율희 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

The CYP2D6 and N-acetyltransferase (NAT2) are polymorphic enzymes which are expressed in the hepatocyte in a genotype-determined manner. CYP2D6 and NAT2 are known to be involved in the inactivation and activation of various mutagens and carcinogens, respectively. The activities of the two enzyme systems are associated with the genetic susceptibility of many human cancers. This study was performed to determine the genotype frequencies of the two enzyme systems in primary hepatocellular carcinoma patients and healthy controls. One hundred healthy controls and 55 liver cancer patients were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The results are as followings:1. In the healthy controls, CYP2D6 wild type allele frequency was 0.985 and CYP2D6^*4 frequency was 0.015, and no CYP2D6 poor-metabolizer was detected. No significant differences were found in hepatocellular carcinoma patient group. 2. Frequencies of F, S1, S2 and S3 alleles of NAT2 system were 0.725, 0.01, 0.14 and 0.125 in controls, respectively. The genotype frequencies were found to be 0.91 of rapid acetylator and 0.09 of slow acetylator. No significant differences were found in hepatocellular carcinoma patient group. 3. These results suggest that the distribution of CYP2D6 and NAT2 polymorphisms is very unique in Korean populations characterized by extremely low frequency of CYP2D6 poor-metabolizer and NAT2 slow acetylator. CYP2D6 and NAT2 polymorphisms seemed not to play an important role in the hepatic carcinogenesis in Korean population.

GT rotary file을 이용한 만곡 근관형성시 작업 효율 및 file 변형 발생에 관한 연구

신주희,백승호,배광식,임성삼,윤수한,김병현 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Root canal preparation process is of utmost importance in successful treatment of root canal. Also, one of the most important purpose of the root canal preparation is to enlarge the root canal three dimensionally without changing the curvature of the root canal. However, as the curvature of the root canal increases, there are many difficulties involved in formation of optimum root canal. Therefore, in order to solve the above mentioned problems, new developments in methods of root canal preparation and equipments for such purposes were made. Recently, vigorous studies about newly introduced engine-driven nitckel-ti-tanium rotary file are conducted. As shown in research results to dates, it is well established that the use of nickel-titanium file is better suited for curved root canal than stainless steel file in maintaining the curvature of root canal and reducing the deformation of root canal. However, it is also acknowledged that there are a few discrepancies in research results according to protocol, due to failure to remove variables in experiments. In addition, although it is recommended by the manufacturer that the GT rotary file should maintain a low rotational speed of 150∼350rpm and 'light pressure' as light as not to break the lead of a pencil, academic studies about the vertical force which is not yet standardized are not sufficiently explored. Therefore, this research devised and utilized a special research equipment to standardize the appropriate range of vertical force for GT rotary file through experiments by breaking of the lead of a pencil as expressed by the manufacturer and to accurately measure factors involved through repeating and recreating the environment of root canal preparation. Forming nine experimental groups by varying the vertical forces (150g, 220g, 300g) and rpm (150rpm, 250rpm, 350rpm), the effects of changing vertical forces and rpm on working efficiency were measured in terms of time expended in root canal preparation by crown-down method using a transparent resin block with 35 degree curvature and GT rotary file (z-test). The following research using this special research equipment that involved nine experimental groups and varying the vertical force for root canal preparation from 300g which is within the normal vertical force range to 700g and 1000g which fall outside the normal rpm range. The results were as follows : 1.Analysis of the experiment results revealed that the tome spent in root canal preparation decreased as the vertical forces and rpm increased (p<0.05). Also, the effects of rpm were greater than those of the vertical forces within the normal vertical force range (β-weight test) . 2.Observation of the deformation of GT rotary file revealed that deformation increases in a direct correlation with the vertical force increase and in a reverse correlation with the rpm decrease, In the case of the vertical forces close to the normal range, the probability of GT rotary file deformation were quite different depending on the rpm changes. In the case of◎eater tier◎cal forces, the occurrences of deformation of the file were more frequent regardless of the rpm changes. 3.Deformation and breakage of file were also commonly observed in the expended time measurement experiments and GT rotary file deformation experiments in which low speed rpm (150rpm) was used and at the curved portion of the resin block. Key words : GT rotary file, Crown-down method, Working efficiency, Vertical force, Rpm, Deformation and breakage of file

운동실조를 동반하지 않은 급성외안근마비 1례

신대수,이승회,이한샘,박성욱,박현영,장혁,김요식,조광호 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

플라즈마 용사 공정의 최적화를 위한 수치해석

신기열,김광수 대구미래대학 2000 論文集 Vol.18 No.1

Numerical analysis of the jet flow which was developed from DC non-transfered plasma torch was investigated for an analysis of thermal plasma spray characteristics. The numerical flow field resulted in a two-dimensional, steady state fluid model where the turbulence effects were considered in the standard K-ε model. The sprayed particle-plasma interaction with dual injection ports are considered.

급성 심근경색 초기에 도플러 심초음파를 이용한 좌심실 이완기 기능의 평가

신원용,김주성,김철현,이광희,최태명,현민수,김성구,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background : Congestive heart failure after acute myocardial infarction relate to left ventricular systolic sysfunction. Also, left ventricular diastolic function contribute to heart failure. The aims of this study were, firstly to characterize left ventricular diastolic function by transmitral flow velocity, and secondly to study the significance of Doppler measurements in relation to the development of heart failure in the early phase of myocardial infarction. Method : Pulsed Doppler echocardiography of transmitral flow was assessed in 34 patients with acute myocardial infarction. According to the Doppler transmitral flwo velocity profile, the study patients were assigned to the three groups: normal filling pattern (DT > 140ms, IVRT < 100ms), relaxation abnormality (DT > 140ms, IVRT ≥ 100ms), pseudonormal or restrictive pattern (DT ≤ 140ms). Also, on the basis of the presence of heart failure during first week of hospitalization, the patients were divided into two groups: patients with no sign of heart failure (Killip class Ⅰ) and heart failure (Killip class Ⅱ-Ⅳ). Results : Of the 34 patients studied, 10(29%) were normal filling pattern, 17(50%) abnormal relaxation and 7(21%) were restrictive patterns. The left ventricular ejection fraction(EF, 40 ±6%) of restrictive pattern was significantly lower than that of normal filling pattern of abnormal relaxation(p < 0.01). And, the IVRT, DT, E/A and left ventricular EF were reduced in patients with heart failure(14 patients) compared to no sign of heart failure(20 patients)(p<0.05). Of the patients with heart failure, IVRT and left ventricular EF were reduced in patients with DT≤140 ms compared to DT> 140ms(p=0.001). Conclusion : Left ventricular diastolic dysfunction is present early after onset of symptoms of acute myocardial infarction. Assessment of left ventricular diastolic function complements measurements of systolic function in the evaluation of cardiac function. DT, IVRT, E/A and left ventricular EF are useful predictors for development of congestive heart failure following acute myocardial infarction.