대기 현상에 대한 초등학생의 선개념 및 오개념 분석

조현희,김광명 춘천교육대학교 과학교육연구소 2001 과학교육연구 Vol.24 No.-

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

원예작물의 염류장애 원인과 대책을 위한 생리, 생화학적 연구 2. 토마토 뿌리조직 H+ 펌프 활성 및 Thapsigargin 저해효과

조광현,사공정,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1997 연구보고서 Vol.2 No.-

Thapsigargin은 동물조직 SR/ER-type Ca²+-ATPase에 특이성이 높은 선택적 저해제로서 토마토 뿌리조직으로부터 분리한 마이크로솜에 Ca²+-ATPase의 존재를 확인하기 위하여 사용되었다. 원형질막과 액포막에 위치하는 H+-ATPase들의 활성은 각각의 선택적 저해제인 vanadate와 NO₃를 이용하여 평가하였고, 이들의 활성은 각각 마이크로솜 ATPase 총활성의 ∼26%, ∼36%로 나타났다. 이들 두 가지 저해제 효과는 additive하게 나타났으며, 전체활성의 약 50∼70%를 저해함을 확인하였다. 마이크로솜 ATPase활성에 대한 thapsigargin의 저해효과에서 10 μM thapsigargin은 총활성의 30%를 저해하였으며, 저해효과는 농도 의존적으로 나타났다. Thapsigargin에 의한 활성저해 기작을 조사하기 위하여 H+-ATPase의 선택적 저해제들을 사용하였다. 액포막의 H+-ATPase 활성에 대한 thapsigargin의 효과를 조사하기 위한 NO₃의 dose-response 실험에서, thapsigargin은 NO₃에 의해 저해되는 ATPase활성을 경쟁적으로 감소시켰다. 반면, vanadate의 농도를 10 nM∼2mM로 증가시켜 원형질막의 H+-ATPase 활성을 저해시킨 조건에서는 thapsigargin에 의하여 저해되는 활성이 온전히 나타나 vanadate의 저해효과와는 무관한 결과를 얻었다. 이러한 결과는 액포막 H+-ATPase의 활성이 thapsigargin에 의해 저해된다는 것을 의미한다. 저자들은 액포막 H+-ATPase의 활성이 Ca²+에 의하여 저해됨을 이미 보고하였다 (Cho et al., 1998). Thapsigargin에 의하여 저해되는 마이크로솜 ATPase활성은 반응용액의 Ca²+농도가 증가할수록 감소하였으며, 이것은 thapsigargin이 액포막 H+-ATPase 활성을 저해한다는 가능성을 더욱 지지하였다. 이상의 결과는 토마토 뿌리조직의 마이크로솜 ATPase중 액포막의 H+-ATPase 활성이 thapsigargin에 의해서 저해되는 것을 보여주며, 액포막 H+-ATPase와 ER-type Ca²+-ATPase와의 구조적, 기능적 연관성이 있을 가능성을 제안한다. The effect of thapsigargin, a SR/ER-type Ca²+-ATPase inhibitor, was investigated to determine the presence of thapsigargin-sensitive Ca²+-ATPases in the microsomes of tomato roots. The activities of plasma membrane H+-APTase and vacuolar H+-ATPase were evaluated to ∼26% and ∼36% of total microsomal ATPase activity by using their specific inhibitors, vanadate and NO₃, respectively. The inhibitory effects of vanadate and NO₃- were additive and the simultaneous additions of these two inhibitors decreased the total activity up to 50∼70%. Thapsigargin (10μM) inhibited the microsomal ATPase activity by ∼30% and the thapsigargin-induced inhibition was appeared to be dose-dependent. In order to understand the molecular mechanism of thapsigargin-induced inhibition, the effects of specific H+-ATPase inhibitors were tested and compared to the effect of thapsigargin. The NO₃-induced inhibition was decreased by simultaneous treatment of thapsigargin. In dose-response experiments of vanadate and NO₃-, thapsigargin only blocked the NO₃-induced inhibition, while the effects of vanadate and thapsigargin were independent at the whole range of vanadate concentration (10 nM∼2 mM). Meanwhile, the thapsigargin-induced inhibition was decreased by increasing Ca²+ concentration. Since we have shown that Ca²+ blocks the activity of vacuolar H+-ATPase, these results together suggest that thapsigargin inhibits the NO₃-sensitive vacuolar H+-ATPase but not the vanadate-sensitive plasma membrane H+-ATPase. Our results also suggest that there is structural and functional relations between vacuolar H+-ATPase and SR/ER-type Ca²+-ATPase.

호스피스 시설의 유형별 공간구성에 관한 건축 계획적 연구

조광현,박재승 한국의료복지시설학회 2002 의료·복지 건축 Vol.8 No.1

Recently cancer, AIDS, chronic sickness have increased according to the elevation of socioeconomic level and fast change of lifestyle. The number of patients receiving terminal care increased fairly because the span of life is extended by development of medicinal technology. Also necessity of hospice and palliative care was risen according to the request of terminal patients that remove pain and keep calm life by interest about quality of life, However architectural plan and type specialization of facility which can correspond team's composition and supplied nursing program are not consisting. This study researches about care environment of hospice facility plan through investigation into terminal patient's special quality. The purpose of this study is to propose fundamental datas of hospice facility for architectural plan through comparative analysis of cases of domestic and outside facilities.

유사체액에서의 전기화학적 처리가 티타늄 표면의 인산갈슘 흡착에 미치는 영향

조국현,김문영,송광엽,배태성,이민호 大韓齒科器材學會 2002 대한치과재료학회지 Vol.29 No.3

This study was performed to examine the effect of electrochemical treatment on the precipitation of apatite-like calcium phosphate in a simulated body fluid. Titanium plates of 20×20×1 ㎣ in dimensions were polished sequentially from #600 to #2,000 emery paper and one surface of each specimen was received an additional polishing sequence through 0.1㎛ alumina paste. To improve the bioactivity of titanium plates, they were electrochemically treated at 100 mA for 20 min in an electrolyte containing the calcium and phosphate ions, and the Hanks' solution with pH 7.4 at 36.5℃ for 30 days, and the surfaces were examined with X-ray diffractometer(XRD), scanning electron microscope(SEM) and X-ray photoemission spectroscopy(XPS). Precipitation of calcium phosphate and CaO were observed on the electrochemically treated titanium surface in a simulated body fluid after treatment at 600℃. Precipitation of calcium phosphate was densified and coarsened with increasing the temperature of the electrolyte solution in the range of 20-80℃. The calcium phosphate contained phosphate in the form of Po₄^3-, HPO₄^2-, and H₂PO₄^-.

HGCMP기반 서버 시스템상의 전달지연 연구

조현주,김한수,이광휘 昌原大學校 基礎科學硏究所 1995 基礎科學硏究所論文集 Vol.7 No.-

In this thesis, the message transmission delay on a server system using HGCMP for group communication protocol was analyzed. The queue model of a server system was modeled by queueing theoretic approach. As the feedback structure was imbedded in the queue model, it is difficult to analyze the delay. To solve this problem, the simplification of the queue model was performed. After the simplification process, the queue model was modeled M/M/1. The transmission delay of a server system is calculated using average waiting time and service time in a server system.

마늘 추출액의 돌연변이 억제효과에 관한 연구

조현,변광의 순천향대학교 1993 논문집 Vol.16 No.1

Garlic extract itself had no mutagenicity and inhibited the mutagenicity of 2-aminofluorene (2-AF) toward salmonella typhimurium TA 98 and TA 100 strains when Ames assay has been carried out. But, in case of benzo(a)pyren as mutagen, the inhibitory effect of garlic extract was not observed. Water extract of garlic inhibited the mutagenicity of 2-AF more effectively than ethanol extract but its inhibitory effect was decreased with heat treatment. Using ultrafiltration method, we could know that the inhibitory factor has the molecular weight below 550 daltons. Also, we saw from adhesion of inhibitory factor to ion exchange resin(Dowex-1-chloride X-8) that this exhibited anionic character. Further study should be in need to conform the characteristics of that factor.

부산 백병원에서 실시한 개심술의 최근 기법

조광현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

■ Background 3,175 cardiac surgeries were performed in the Pusan Paik Hospital from Sep. 1985 to April 2002, and recently several kinds of current techniques for open heart surgery were adopted with good results. ■ Method Retrospective analysis of the cases and review of the results of operation and usefulness of current techniques such as OFF-Pump CABG. ARCH-FIRST TECHNIQUE. MICRO-WAVE ABLATION, TRANSPLANTATION and MINIMAL INCISION were done. ■ Results ① Among the total cases (3,179), open heart and non-open heart surgery cases were 1,988 and 218, respectively. ② 188 cases of CABG were done. Among them, 20 cases underwent OFF-PUMP coronary artery bypass surgery. 21 cases underwent complete arterial revascularization, and 14 cases underwent both. ③ 75 cases of aortic aneurysm (ascending or arch aneurysm) were operated undercardiopulmonary bypass. The causes of these aneurysm were dissecting aneurysm (45), annuloaortic ectasia(24), non-dissecting chronic aneurysm(5). In the surgery of arch aneurysm. 7 cases underwent ARCH-FIRST TECHNIQUE with good results. ④ In the treatment of atrial fibrillation coupled with valvular heart disease. MICRO-WAVE ABLATION were performed in 18 cases with 83.3 % sinus rhythm recovery. ⑤ Four cases of heart transplantation were performed, using Shumway method, with 3 survivals and one death. ⑥ Minimally invasive incision (using lower sternal incision) was performed successfully in the cases of tricuspid valve diseases(10) and congenital septal defects (50). ■ Conclusion The over all postoperative hospital mortality with open heart surgery was not so high as 3.5 %, whereas more proper management of the all patients with current techniques must be continued.

MRI용 초전도 능동차폐 마그네트의 설계에 관한 연구

조윤현,이광호,김성도 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

An optimal design approach is presented for the high homegeneity superconducting magnet with a superconducting active shield esperically for a magnetic resonance imaging system. This paper is investigated magnetic phenomena for a stray magnetic to get reduction technigues of the unwanted stray magnetic field from the superconducting magnet.

원예작물의 염류장애 원인과 대책을 위한 생리, 생화학적 연구 3.토마토 뿌리조직에서 마이크로솜 Ca²+ uptake 특성연구

조광현,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

식물세포의 세포질 Ca²+ 이동과 관련된 Ca²+ transport 특성을 조사하기 위하여, 토마토 뿌리조직으로부터 마이크로솜을 분리하고, 45Ca²+ uptake 실험을 수행하였다. 반응용액에 Ca²+-ATPase의 선택적 저해제인 1 mM vanadate와 액포막 H+-ATPase의 선택적 저해제인 50 mM NO₃를 각각 첨가하였을 때, 45Ca²+ uptake는 각각 20%와 30% 저해되었고, 두 가지 저해제를 동시에 첨가하였을 때 50% 저해되었다. 또한 마이크로솜 45Ca²+ uptake는 vanadate 또는 NO₃-의 농도를 증가시킴에 따라 저해되었다. 이러한 저해효과는 토마토 뿌리조직 마이크로솜에 Ca²+-ATPase와 Ca²+/H+ antiport가 동시에 존재할 가능성을 시사한다. Protonophore인 gramicidin의 처리에 의해 45Ca²+ uptake는 30% 가량 저해되어, 토마토 뿌리조직 마이크로솜에 Ca²+/H+ antiport가 존재하고 있음을 확인하였다. NO₃-를 처리하여 45Ca²+ uptake를 저해시킨 후 gramicidin을 처리하였을 때, gramicidin에 의한 추가저해는 거의 관측되지 않았으나, vanadate의 경우 gramicidin에 의한 추가저해가 현저히 나타났다. 이러한 결과는 토마토 뿌리조직 마이크로솜 45Ca²+ uptake가 vanadate에 의하여 저해되는 Ca²+-ATPase와 NO₃-에 의하여 저해되는 Ca²+/H+ antiport에 의해 이루어지고 있음을 의미한다. 한편, 동물조직 ER/SR-type Ca²+-ATPase의 특이성 높은 선택적 저해제인 thapsigargin은 마이크로솜 45Ca²+ uptake를 농도 의존적으로 저해하였으며, 10μM 농도에서 최대저해효과를 나타냈다. Thapsigargin에 의한 45Ca²+ uptake의 저해효과는 NO₃를 사용하여 액포막 H+-ATPase 활성을 저해하였을 때 현저하게 감소하였다. 반면, vanadate를 처리하여 원형질막 H+-ATPase의 활성을 저해한 후에도 thapsigargin에 의한 추가적 저해효과가 관측되어 thapsigargin이 액포막 H+-ATPase의 활성을 저해하여 간접적으로 Ca²+/H+ antiport를 저해함을 확인하였다. 이상의 결과들은 토마토 뿌리조직 마이크로솜에 Ca²+-ATPase와 액포막 Ca²+/H+ antiport가 존재하며, thapsigargin이 액포막 Ca²+/H+ antiport를 선택적으로 저해함을 보여준다. In order to characterize the property of Ca²+ transport in plant tissue, microsomes were prepared from the roots of tomato and microsomal 45Ca²+ uptake was measured. When 1mM vanadate, a selective inhibitor of P-type ATPases, 50 mM NO₃, a specific inhibitor of vacuolar H+-ATPase, and both of these inhibitors were treated, the microsomal 45Ca²+ uptakes were inhibited by 20%, 33%, and 47%, respectively. The inhibitory effects of these two inhibitors were investigated by using a protonophore, gramicidin. When the chemical gradient of H+ was relieved by gramicidin, the uptake was decreased by 30%, implying the presence of Ca²+/H+ antiporter in the microsomal membrane. In the 45Ca²+ uptake experiment, the effect of gramicidin was independent of vanadate-induced inhibition. However, when the activity of vacuolar H+-ATPase was inhibited by NO₃, the effect of gramicidin was severely decreased. Meanwhile, thapsigargin, a specific antagonist of ER/SR-type Ca²+-ATPase, inhibited the microsomal 45Ca²+ uptake and the maximum inhibitory effect was obtained at 10μM. The effect of thapsigargin was blocked by NO₃and gramicidin, but not by vanadate. These results imply that vanadate directly inhibits the activity of Ca²+-ATPase; however, NO₃- and thapsigargin block the activity of Ca²+/H+ antiporter by inhibiting the vacuolar H+-ATPase. In conclusion, the microsomal 45Ca²+ uptakes are mediated by two major enzymes, Ca²+-ATPase and Ca²+/H+ antiporter in tomato root tissue.