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Yan Wang,Mary Yuk Kwan Lee,Judith Choi Wo Mak,Mary Sau Man Ip 대한당뇨병학회 2019 Diabetes and Metabolism Journal Vol.43 No.5
Background: The relationship between obstructive sleep apnoea (OSA) and metabolic disorders is complex and highly associated. The impairment of adipogenic capacity in pre-adipocytes may promote adipocyte hypertrophy and increase the risk of furthermetabolic dysfunction. We hypothesize that intermittent hypoxia (IH), as a pathophysiologic feature of OSA, may regulate adipogenesisby promoting macrophage polarization. Methods: Male C57BL/6N mice were exposed to either IH (240 seconds of 10% O2 followed by 120 seconds of 21% O2, i.e., 10 cycles/hour) or intermittent normoxia (IN) for 6 weeks. Stromal-vascular fractions derived from subcutaneous (SUB-SVF) and visceral(VIS-SVF) adipose tissues were cultured and differentiated. Conditioned media from cultured RAW 264.7 macrophages afterair (Raw) or IH exposure (Raw-IH) were incubated with SUB-SVF during adipogenic differentiation. Results: Adipogenic differentiation of SUB-SVF but not VIS-SVF from IH-exposed mice was significantly downregulated incomparison with that derived from IN-exposed mice. IH-exposed mice compared to IN-exposed mice showed induction of hypertrophicadipocytes and increased preferential infiltration of M1 macrophages in subcutaneous adipose tissue (SAT) comparedto visceral adipose tissue. Complementary in vitro analysis demonstrated that Raw-IH media significantly enhanced inhibition ofadipogenesis of SUB-SVF compared to Raw media, in agreement with corresponding gene expression levels of differentiation-associatedmarkers and adipogenic transcription factors. Conclusion: Low frequency IH exposure impaired adipogenesis of SAT in lean mice, and macrophage polarization may be a potentialmechanism for the impaired adipogenesis.
Sun, Yan-Lin,Wang, Dong,Yeom, Myung-Hun,Kim, Duck-Hee,Kim, Han-Gon,Hong, Soon-Kwan The Korean Society of Plant Biotechnology 2011 식물생명공학회지 Vol.38 No.4
The medicinal herb Oldenlandia diffusa is known as a folk medicine for the treatment of hepatitis, sore throat, appendicitis, malignant tumors and urethral infection in Southern China and Korea. Another species O. corymbosa, is also used for the therapy of the similar conditions, however, only O. diffusa is referred to the medicinal herb by Chinese Pharmacopoeia. Due to their similar morphology, O. diffusa and O. corymbosa are often misidentified. To easily identify O. diffusa from O. corymbosa, the phylogenetic utility of nuclear ribosomal DNA (nrDNA) internal transcribed spacers (ITS) were investigated among different O. diffusa and O. corymbosa populations in Korea. The nrDNA ITS sequence of O. diffusa contained 791 bp, with GenBank accession number of JF837601-JF837602. The nrDNA ITS sequence of O. corymbosa was 785-786 bp, with GenBank accession number of JF837603-JF837611. The results showed that there are some certain divergences in the ITS region sequence between both species, even among different populations of the same species. Particularly, O. corymbosa ST-4 population showed the highest dissimilarity of the ITS region sequence with other nine populations of O. corymbosa and two populations of O. diffusa. This consequence makes us further understand the molecular diversification between O. corymbosa and O. diffusa, and help to promote the correct use and safety.