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      • Output feedback based event-triggered sliding mode control for delta operator systems

        Kumari, Kiran,Bandyopadhyay, Bijnan,Kim, Kyung-Soo,Shim, Hyungbo Elsevier 2019 Automatica Vol.103 No.-

        <P><B>Abstract</B></P> <P>In this paper, we present an output feedback based design of event-triggered sliding mode control for delta operator systems. For discrete-time systems, multi-rate output sampling based state estimation technique is very useful if the output information is available. But at high sampling rates, the discrete-time representation of the system using shift operator becomes numerically ill-conditioned and as a result, the observability matrix becomes singular as the sampling period tends to zero. Here, a new formulation of multi-rate state estimation (MRSE) for a small sampling period is presented. We first propose a new observability matrix and then discuss its relationship with the observability matrix defined in the conventional sense. For the delta operator system with matched uncertainty, we have presented the design of MRSE based sliding mode control (SMC). Additionally, to make the control efficient in terms of resource utilization, MRSE based event-triggered SMC is proposed. The absence of Zeno phenomenon is guaranteed as the control input is inherently discrete in nature. Finally, the effectiveness of the proposed method is illustrated through numerical simulations, considering a ball and beam system and a general linear system as a numerical example.</P>

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        The transcriptome response of Enterobacter sp. S-33 is modulated by low pH-stress

        Kumari Kiran,Sharma Parva Kumar,Singh Rajnish Prakash 한국유전학회 2024 Genes & Genomics Vol.46 No.6

        Background Acidic environments naturally occur worldwide and uncontrolled use of agricultural practices may also cause acidification of soils. The development of acidic conditions disturbs the establishment of efficient microbial populations in their natural niches. The survival of Enterobacter species under acidic stress remains poorly understood. Objective This study aimed to investigate the survival of an environmental isolate Enterobacter sp. S-33 under acidic stress and to identify the various genes involved in stress protection at the global gene transcription level. The obtained results provide new targets that will allow understanding the in-depth mechanisms involved in the adaptation of bacteria to environmental pH changes. Methods We used the next-generation sequencing (NGS) method to analyze the expression (up-regulation & down-regulation) of genes under varying pH conditions. Results A total of 4214 genes were differentially expressed under acidic conditions (pH 5.0), with 294 up-regulated and 167 down-regulated. At pH 6.0, 50 genes were significantly expressed, of which 34 and 16 were identified as up-regulated and down-regulated, respectively. Many of the up-regulated genes were involved in carbohydrate metabolism, amino acid transport & metabolism, and the most down-regulated genes were related to post-translational modification, lipid transport & metabolism, etc. The observed transcriptomic regulation of genes and pathways identified that Enterobacter reduced its post-translational modification, lipid transport & metabolism, and increased carbohydrate metabolism, amino acid metabolism & transport, energy production & conversion to adapt and grow in acidic stress. Conclusions The present work provides in-depth information on the characterization of genes associated with tolerance or adaptation to acidic stress of Enterobacter bacterium. Background Acidic environments naturally occur worldwide and uncontrolled use of agricultural practices may also cause acidification of soils. The development of acidic conditions disturbs the establishment of efficient microbial populations in their natural niches. The survival of Enterobacter species under acidic stress remains poorly understood. Objective This study aimed to investigate the survival of an environmental isolate Enterobacter sp. S-33 under acidic stress and to identify the various genes involved in stress protection at the global gene transcription level. The obtained results provide new targets that will allow understanding the in-depth mechanisms involved in the adaptation of bacteria to environmental pH changes. Methods We used the next-generation sequencing (NGS) method to analyze the expression (up-regulation & down-regulation) of genes under varying pH conditions. Results A total of 4214 genes were differentially expressed under acidic conditions (pH 5.0), with 294 up-regulated and 167 down-regulated. At pH 6.0, 50 genes were significantly expressed, of which 34 and 16 were identified as up-regulated and down-regulated, respectively. Many of the up-regulated genes were involved in carbohydrate metabolism, amino acid transport & metabolism, and the most down-regulated genes were related to post-translational modification, lipid transport & metabolism, etc. The observed transcriptomic regulation of genes and pathways identified that Enterobacter reduced its post-translational modification, lipid transport & metabolism, and increased carbohydrate metabolism, amino acid metabolism & transport, energy production & conversion to adapt and grow in acidic stress. Conclusions The present work provides in-depth information on the characterization of genes associated with tolerance or adaptation to acidic stress of Enterobacter bacterium.

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      • KCI등재

        Invariant Submanifolds of N(k)-contact metric manifolds with generalized tanaka webster connection

        Dipansha Kumari,H.G. Nagaraja,D.L. Kiran Kumar 한국전산응용수학회 2022 Journal of applied mathematics & informatics Vol.40 No.3

        The object of the present paper is to study some geometric properties of invariant submanifolds of N(k)-contact metric manifold admitting generalized Tanaka-Webster connection.

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