Expression Profiles of Retinoblastoma Protein and p27Kip1 According to HER2 Amplification Status in Primary Breast Cancer

신재호,Sung-Jin Park,Hyun-Jin Cho,Geumhee Gwak,Byung-Noe Bae,Ki Whan Kim,Hong-Yong Kim2,Kyeongmee Park,Sehwan Han 한국유방암학회 2009 Journal of breast cancer Vol.12 No.1

Purpose: Human epidermal growth factor receptor-2 (HER-2)/neu amplification affects the cell proliferation through the modulation of multiple G1 cell cycle regulators in breast tumor cells. We performed this study to investigate whether retinoblastoma protein (pRB) and p27Kip1 were differently expressed according to the HER2 amplification status in human breast cancer. Methods: HER2 amplification was assayed by fluorescence in situ hybridization and the expression of cell cycle regulators were assayed by immunohistochemistry on 153 consecutive invasive breast cancers. The proliferative activity of breast cancer was analyzed according to the HER2 amplification and cell cycle protein expression status. Results: HER2 amplification was observed in 39 (25.5%) of 153 breast cancers. In the HER2 amplified breast cancers, the pRB expression was significantly increased (p=0.011) whereas there was no significant relationship between HER2 amplification and p27Kip1 expression. There was an inverse correlation between pRB expression and Ki-67 labeling index in the HER2 amplified breast cancers (p=0.036). In contrast, Ki67 labeling index was significantly decreased as p27Kip1 expression increased in HER2 non-amplified breast cancers (p=0.028). In HER2 non-amplified breast cancers, we could not observe any association between the pRB expression and Ki67 labeling index. Conclusion: The proliferation of the breast cancers was associated with pRB expression in HER2 amplified tumors whereas it was associated with p27Kip1 expression in HER2 non-amplified tumors. The results of the current study indicate that the cell proliferative activity of the breast cancer is under different growth signal pathways according to HER2 amplification status.

Activation of mast cells is dependent on tyrosine phosphorylation of Phospholipase D2

Kim Young Mi,Lee Jun Ho,Seo Jin Young,Kim Nam Wook,Chang Sung Ho,Ko Na Young,Her Erk,Lee Hoi Young,Han Jeung Whan 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.2

Preclinical development of a humanized neutralizing antibody targeting HGF

Kim, Hyori,Hong, Sung Hee,Kim, Jung Yong,Kim, In-Chull,Park, Young-Whan,Lee, Song-Jae,Song, Seong-Won,Kim, Jung Ju,Park, Gunwoo,Kim, Tae Min,Kim, Yun-Hee,Park, Jong Bae,Chung, Junho,Kim, In-Hoo Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.3

<P>Hepatocyte growth factor (HGF) and its receptor, cMET, play critical roles in cell proliferation, angiogenesis and invasion in a wide variety of cancers. We therefore examined the anti-tumor activity of the humanized monoclonal anti-HGF antibody, YYB-101, in nude mice bearing human glioblastoma xenografts as a single agent or in combination with temozolomide. HGF neutralization, The extracellular signal-related kinases 1 and 2 (ERK1/2) phosphorylation, and HGF-induced scattering were assessed in HGF-expressing cell lines treated with YYB-101. To support clinical development, we also evaluated the preclinical pharmacokinetics and toxicokinetics in cynomolgus monkeys, and human and cynomolgus monkey tissue was stained with YYB-101 to test tissue cross-reactivity. We found that YYB-101 inhibited cMET activation <I>in vitro</I> and suppressed tumor growth in the orthotopic mouse model of human glioblastoma. Combination treatment with YYB-101 and temozolomide decreased tumor growth and increased overall survival compared with the effects of either agent alone. Five cancer-related genes (TMEM119, FST, RSPO3, ROS1 and NBL1) were overexpressed in YYB-101-treated mice that showed tumor regrowth. In the tissue cross-reactivity assay, critical cross-reactivity was not observed. The terminal elimination half-life was 21.7 days. Taken together, the <I>in vitro</I> and <I>in vivo</I> data demonstrated the anti-tumor efficacy of YYB-101, which appeared to be mediated by blocking the HGF/cMET interaction. The preclinical pharmacokinetics, toxicokinetics and tissue cross-reactivity data support the clinical development of YYB-101 for advanced cancer.</P>

Isolation and Characterization of Bio-active Materials from Prickly Lettuce (Lactuca serriola)

Sung-Il Moon,Sung-Whan Kim,Wan Huh,Soon-Young Kim,Jong-Sik Kim,Kon-Joo Lee 한국생명과학회 2008 한국생명과학회 심포지움 Vol.49 No.-

A Quantiative Comparison of Vaccinia virus Shedding from Conventional Dressing Sites and Vaccination Lesions after Smallpox Vaccination

Kim, Sung-Han,Yeo, Sang-Gu,Cho, Jae-Hyun,Bang, Ji-Whan,Kim, Hong-Bin,Kim, Nam-Joong,Jee, Youngmee,Cho, Haewol,Oh, Myoung-don,Choe, Kang-Won 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

목적 : 두창백신 접종 후 일반적으로 접종 부위에 거즈를 먼저 덮고, 그 위에 반투과막을 덮어주는 드레싱을 권장하고 있다. 저자들은 이러한 드레싱 방법의 효율성을 알아보기 위해서 두창백신 접종 후 접종 부위, 거즈 바깥부위, 반투과막 바깥 부위에서 각각 분비되는 바이러스양을 정량적으로 비교하였다. 재료 및 방법 : Lancy-Vaxina^((R)) (Berna Biotech, Swiss) 두창백신 1:10 희석연구에 참여한 자원자들 중에서 접종부위에 정해진 기간에 검체 채취에 동의한 40명을 대상으로 연구를 진행하였다. 참가자들은 두창백신 접종 후 6, 8, 10, 13, 15일에 각각 방문하여 드레싱을 교체하였다. 드레싱을 교체하면서 두창백신 접종 부위, 거즈 바깥부위, 반투과막 바깥부위에 면봉을 이용해서 각각 검체를 채취하였다. 검체는 냉동 보관한 후 plaque assay를 통하여 정량적으로 바이러스 역가를 측정하였다. 결과 : 총 40명의 자원자가 본 연구에 참가하였다. 백신 접종 부위에서 채취한 156 검체 중 126건(81%)에서 백시니아바이러스가 검출되었다. 백신 접종 부위에서 접종 8일째 바이러스 역가의 평균(geometric mean titer(log_(10))은 3.91 pfu/mL이었다. 거즈 바깥부위에서 총 39검체가 얻어졌고, 이 중 16건(41%)에서 백시니아바이러스가 검출되었다. 거즈 바깥부위에서 접종 8일째 바이러스 역가의 평균은 0.91 pfu/mL이었다. 반투과막 바깥부위에서 총 133 검체가 얻어졌고, 이 중 1건(0.8%)에서 백시니아바이러스가 검출되었다. 접종 8일째 거즈 바깥부위에서는 바이러스가 검출되지 않았다. 결론 : 두창백신 접종 후에 거즈를 덮고 그 위에 반투과막을 덮어주는 드레싱 방법은 백시니아바이러스 배출을 효과적으로 줄여줄 수 있음을 확인하였다. Background : We compared vaccinia virus shedding from the vaccine inoculation site (vaccination lesion) and two sites of a dressing covering the vaccination site; the outer surface of the semipermeable dressing (outer surface) and the inner surface of the semipermeable dressing, that is, the surface of a folded gauze under the semipermeable membrane (gauze surface) Material and Methods : Subjects were recruited from the volunteers who participated in a clinical trial of the efficacy of a 1:10 dilution of Lancy-Vaxina^((R)) (Berna Biotech, Switzerland), and were seen every 2-3 days (days 6, 8, 10, 13, and 15 after smallpox vaccination) for scheduled dressing changes. Swab specimens were obtained from the vaccination lesion, the outer surface, and the gauze surface. Quantitative viral culture assays for these specimens were done. Results : Vaccinia virus was recovered from 126 (81%) of the 156 vaccination lesion samples collected from the 40 participants. A high virus titer was recovered from the vaccination lesion (geometric mean titer (log_(10))=3.91 on day 8). Of the 39 swab samples obtained from the gauze surface of the gauze, 16 (41%) were positive for virus. An intermediate titer was recovered from the gauze surface (geometric mean titer (log_(10))=0.91 on day 8). Of the 133 swab samples obtained from the outer surface, only one (0.8%) was positive for vaccinia. No virus was recovered from the outer surface on day 8. Conclusion : Our findings suggest that the addition of a semipermeable dressing to the folded gauze further reduces viral shedding and therefore increases protection.

Is the Oscillometric blood pressure measuring device in accord with the intra-arterial invasive blood pressure measuring method ?

Kim, Gi Hyun,Joo, Hye Jin,Lee, Won-luk,Lee, Ju-Hee,Lee, Sang Yeub,Bae, Jang-Whan,Hwang, Kyung-Kuk,Kim, Dong-Woon,Cho, Myeong-Chan 충북대학교 의과대학 충북대학교 의학연구소 2010 忠北醫大學術誌 Vol.20 No.1

Purpose: Hypertension is closely related to the occurrence and aggravation of major incidents in cardiovascular system. However, there is not much elucidated about the accordance of blood pressure (BP) measurement with oscillometric device and invasice BP from patients having the risks of cardiovascular disease. We have checked the accordance of non-invasive BP using the oscillometric device in comparison with invasive BP through redial artery catheter in performing coronary angiography. Subjects and methods: We compared the BP measured through redial artery and electronic sphygomanometer (Omron HEM-747IC, Japan) from the brachial for 104 patients who had gone through coronary artery angiography by visiting Chungbuk university hospital from September, 2007 till April, 2008. Results: The BP measured with oscillometric device was mostly in accordance with invasive BP for all systolic, diastolic and average BP. However, the group of stage 1 hypertension by the standards of Seventh Report of the Joint National Committee showed low accordance between the two methods in diastolic BP. For non hypertension group and pre-hypertension group, systolic BP showed a pattern where systolic BP was underestimated, but for the first and second stage hypertension groups, the BP measured with oscillometric device displayed an overestimated patten Conclusion: Most of the blood pressures measured with automated sphygomanometer showed good agreement with invasive BP. However, it needs attention in using because the diastolic BP is not in accordance with invasive BP form the first stage hypertension patients. 연구 목적 : 고혈압은 심혈관계 주요사건 발생과 악화에 밀접한 관계가 있다. 그러나 아직까지 심혈관 질환의 위험성을 가진 환자에서 전자 혈압계를 통한 혈압 측정과 침습적 혈압과 일치여부에 대해서 잘 밝혀진 바가 없다. 우리는 관상동맥 조영술을 실시하면서 노동맥 도관을 통한 침습적 혈압과 비교하여 전자열합계를 이용한 비침급적 혈압의 일치도를 확인하였다. 대상 및 방법 : 2007년 9월부터 2008년 4월까지 충북대학교 병원을 방문하여 관상동맥 조영술을 시행 받은 104명의 환자들을 대상으로 노동맥을 통한 혈압 측정과 상완에서 전자 혈압계 (Omron HEM-747IC, 일본)로 측정한 혈압을 비교하였다. 결과 : 자동 혈압계로 측정한 혈압은 대체로 수축기, 확장기 및 평균 혈압 모두 침습적 혈압 값과 일치하였다. 그러나 Seventh Report of the Joint National Committee 기준으로 1단계 고혈압을 갖는 집단은 이완기 혈압이 저평가되는 양상을 보였으니 1단계 및 2단계 고혈압 군에서는 자동 혈압계로 측정한 혈압이 고평가되었다. 확장기 혈압 및 평균 혈압은 모든 단계에서 고평가 되는 양상을 보였다. 결론 : 자동 혈압계로 측정한 혈압의 대부분은 침습적 혈압과 좋은 일치도를 보인다. 그러나 1단계 고혈압 환자군에서는 확장기 혈압이 침습적 혈압과 일치하지 않으므로 사용에 주의가 필요하겠다.

Studies on the Vase-life of Cut Rose (Rosa hybirda L.) by Various Factors Ⅰ. Effect of Light, Dark, and Number of Leaves on Vase-life of Cut Rose

Kim, Jae-Cheol,Han, Kwang-Soo,Kwon, Sung-Whan 全北大學校 1988 論文集 Vol.30 No.-

절화 장미의 vase-life에 미치는 제요인을 조사하여 적절한 수명연장방법을 구명하기 위한 기초실험으로 장미품종 Super Star를 공시하여 광 · 음 조건하에서 엽수를 달리 한 후 증류수에 보존하였다. 그 후 24시간 간격으로 절화장미의 수분흡수율과 손실율, 생체중의 변화 및 절화수명을 조사하였던 바 얻어진 결과는 다음과 같다. 절화 장미의 수분흡수 및 손실율은 광조건하에서 처음 4일동안 계속적으로 증가되다가 그 후 감소된 반면, 암상태에서는 실험 첫날 이후 계속 감소하는 경향을 나타내었다. 엽수를 달리한 절화 장미를 7일동안 보존하였을 때 광 · 암조건은 수분흡수 및 손실율에 중요한 영향을 미치는 것으로 나타났다. 절화장비의 생체중은 처음 증가하였으나 그 후 계속 감소하였으며, 생체중의 손실은 수분흡수율의 감소보다 먼저 발생하였다. 절화장미의 엽수는 광 · 암상태하에서 공히 수분흡수 및 손실율에 가장 크게 영향하는 요인이었다.

Rheumatoid Arthritis and HLA Class II Genotypes in Korean

Kim, Jung-Man,Lee, Hee-Dai,Han, Chang-Whan,Kim, Jin-Young,Kim, Weon-Yoo,Sung, Jin-Hyung,Ryu, Jae Duk,Ko, Lak-Hyun 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-

To determine which HLA antigens are associated with rheumatoid arthritis in Korean, we studies the HLA class II genotypes in 40 patients with rheumatoid arthritis and 35 normal healthy persons by the two step polymerase chain reaction. At first we designed 20 pairs of group specific primers followed by the alleles which have the same nucleotide sequences in the exon 2 region. We performed amplification reaction with these 20 pairs of primers for 32 cycles. Recation products were electrophoresed and only specifically amplified products were reamplified for 15 cycles with total 76 pairs of allele specific primers. We typed the HLA class II genotypes with specific band patterns of each allele. The genotypes in patients were compared with those of normals. HLA-DR4 gave the highest relative risk for rheumatoid arthritis(RR=4), which was statistically significant(P<0.01). The frequency of HLA-DR4 was 75.0% in patient group and 42.9% in normal control group. Relative risk was also associated with HLA-DQw4 of DQB1 gene(RR=3.82), which was statistically significant(P<0.05). The frequency of HLA-DQw4 was 38.9% in patients group and 14.3% in normal control group. HLA-DR1 and DQw3 were not associated with rheumatoid arthritis(P>0.05), Among HLA-DR4 subtypes, the frequency of Dw14(0404) in patient group was relatively higher (19.2%) than that in normal control group(7.7%). The relative risk was high(2.85), although it was not statistically significant(P>0.05). From this study, HLA-DR4 was found to be highly associated while DRI was not associated with rheumatoid arthritis patients in Koreans. (The Journal of Korean Orthopaedic Research Society 2(1):61-67, 1999)

Hypoplasia of the Right Hepatic Lobe : A case report

Kim, Cheong-Yong,Im, Jeong-Keun,Min, Young-Don,Kim, Sung-Whan 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

간담도계의 해부학적 변이는 흔하나 대부분이 간외담도계나 혈관계에서 발생하고 간자체의 구조적 변이는 드물다. 여러 가지의 간의 구조적 변이중 간엽의 형성 부전은 주로 좌엽에서 발생하며 우엽은 매우 드물다. 이에 저자들은 최근에 49세 여자 환자에서 간 좌엽과 담낭에 결석을 지닌 간 우엽 형성부전을 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Developmental anatomical anomalies are not uncommon in hepatobiliary system. These variations occur mainly in the extrahepatic biliary tract and its vascular tree, but the liver itself is free of major anatomical anomaly. Among varied liver anomalies, hypoplaisa of the left lobe is not rare but only six cases of hypoplasia of the right lobe have been reported. Authors present a case of 49-year-old female of the right lobe hypoplasia with the counterclockwise rotation of the enlarged left lobe and the abnormal location of gallbladder at surgery and review the literatures.

Growth Characteristics as Affected by Polyethylene Film-Mulching in Sesame

Sung Woo Lee,Churl Whan Kang,Dong Hwi Kim,Kang Bo Shim,Nak Sul Seong 韓國作物學會 2002 Korean journal of crop science Vol.47 No.4

This study was carried out to investigate varietal differences on growth characteristics under the conditions of PE film-mulching and non-mulching in sesame. At maturing stage from 76 to 95 days after sowing, Yangbaeckkae, non-branching plant type, under non-mulching showed larger leaf area index (LAI) than that of film-mulching, while plant height and the number of capsules per plant were similar to those of film-mulching. LAI of Ahnsankkae, branching plant type, under non-mulching was similar to film-mulching, while plant height and the number of capsules per plant were smaller than those of film-mulching. Net assimilation rate (NAR) of two varieties under non-mulching was lower at seedling stage from 25 to 35 days after sowing but higher at flowering stage from 45 to 55 days after sowing. At maturing stage from 66 to 77 days after sowing, NAR and crop growth rate (CGR) of Yangbaeckkae under non-mulching were greater than those of film-mulching, whereas those of Ahnsankkae under non-mulching were lesser than those of film-mulching. Yield under non-mulching was decreased by 7 % in Yangbaeckkae and 33 % in Ahnsankkae compared with that of film-mulching, therefore Yangbaeckkae was more adaptable for non-mulching than Ahnsankkae. Main factors decreasing yield of Yangbaeckkae under non-mulching were small LAI, NAR, and CGR at the stage of young seedling, and small number of capsules at early maturing stage from first flowering to 20 days after first flowering.