랜덤화 블록 계획법에서 위치를 이용한 비모수 검정법

심수진,김동재,Sim, Sujin,Kim, Dongjae 한국데이터정보과학회 2013 한국데이터정보과학회지 Vol.24 No.6

반복이 있는 랜덤화 블록 계획법에서 우산형 대립가설을 검정하기 위한 비모수 방법에는 Kim과 Kim (1992)이 제안한 방법이 있다. 본 논문에서는 Orban과 Wolfe (1982)가 제안한 위치와 Kim (1999)이 제안한 대조군과 처리군의 방법을 확장하여 랜덤화 블록 계획법에서 우산형 대립가설에서의 검정법을 제안하였다. 또한 여러 분포에 대한 모의실험 통하여 기존의 방법과의 검정력을 비교하였다. Kim and Kim (1992) proposed typical nonparametric method for umbrella alternative in randomized block design with replications. In this paper, We consider a test procedure for umbrella alternatives in a randomized block design using extension of the two sample placement tests described in Orban and Wolfe (1982) and treatment tests described in Kim (1999). We perform a Monte Carlo study to compare the empirical powers of the test statistics for underlying distributions.

한국어교육에서 다중언어능력 및 다중문화능력의 개념과 적용

조수진 ( Cho¸ Sujin ),김태나 ( Kim¸ Taena ),강승혜 ( Kang¸ Seunghae ) 한국언어문화교육학회 2021 언어와 문화 Vol.17 No.3

This study examines the concept of plurilingual and pluricultural competence presented by the CEFR(2018) and proposes application methods in Korean language education. In Korean language education, learners can improve their competence to target language and culture by understanding that learners have various languages and cultural backgrounds and utilizing such resources and experiences. To this end, the understanding and acceptance of culture can be facilitated by establishing a pluricultural repertoire. The target language can be learned by utilizing various language resources through plurilingual understanding of plurilingualism. Furthermore, by building a plurilingual repertoire, it is possible to take advantage of the language resources that learners have to take the initiative in language learning. By applying this concept, a teaching-learning method can be proposed so that learners can utilize each other's language and cultural resources in professor learning, which used only Korean, the target language in Korean education. (Sogang UniversityㆍSogang UniversityㆍYonsei University)

Age-dependent gait abnormalities in mice lacking the <i>Rnf170</i> gene linked to human autosomal-dominant sensory ataxia

Kim, Youngsoo,Kim, Seong Hun,Kim, Kook Hwan,Chae, Sujin,Kim, Chanki,Kim, Jeongjin,Shin, Hee-Sup,Lee, Myung-Shik,Kim, Daesoo IRL Press 2015 Human molecular genetics Vol.24 No.25

<P>Really interesting new gene (RING) finger protein 170 (RNF170) is an E3 ubiquitin ligase known to mediate ubiquitination-dependent degradation of type-I inositol 1,4,5-trisphosphate receptors (ITPR1). It has recently been demonstrated that a point mutation of <I>RNF170</I> gene is linked with autosomal-dominant sensory ataxia (ADSA), which is characterized by an age-dependent increase of walking abnormalities, a rare genetic disorder reported in only two families. Although this mutant allele is known to be dominant, the functional identity thereof has not been clearly established. Here, we generated mice lacking <I>Rnf170</I> (<I>Rnf170<SUP>−/−</SUP></I>) to evaluate the effect of its loss of function <I>in vivo</I>. Remarkably, <I>Rnf170<SUP>−/−</SUP></I> mice began to develop gait abnormalities in old age (12 months) in the form of asynchronous stepping between diagonal limb pairs with a fixed step sequence during locomotion, while age-matched wild-type mice showed stable gait patterns using several step sequence repertoires. As reported in ADSA patients, they also showed a reduced sensitivity for proprioception and thermal nociception. Protein blot analysis revealed that the amount of Itpr1 protein was significantly elevated in the cerebellum and spinal cord but intact in the cerebral cortex in <I>Rnf170<SUP>−/−</SUP></I> mice. These results suggest that the loss of <I>Rnf170</I> gene function mediates ADSA-associated phenotypes and this gives insights on the cure of patients with ADSA and other age-dependent walking abnormalities.</P>

A study of preflare activity using Hinode XRT: Comparison with the previous study using Yohkoh SXT

Sujin Kim,Y.-J. Moon,Y.-H. Kim,G. S. Choe,K.-S. Kim,Y.-D. Park 한국천문학회 2007 天文學會報 Vol.32 No.1

Development of Eggshell Membrane/Hydroxyapatite-Based Functional Engineering Platforms for Healthcare Applications

( Sujin Kim ),( Sunho Park ),( Daun Kim ),( Yonghyun Gwon ),( Jangho Kim ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.2

Eggshell membrane (ESM) is an agricultural by-product and bioresource, and it exists between egg white and eggshell. Recently, ESM is being recognized as a new biomaterial in biomedical fields (e.g., therapeutic agents for skin wound healing). ESM has entangled micro-fibrous structure with micro-pores, and it is mainly composed of abundant proteins, such as collagen I, V, X, and osteopontin. Also, the surfaces of ESM have many functional chemical groups including amine, carboxyl, and amide groups. However, the raw ESM has many limitations of the restricted scale-up platforms, low mechanical property and poor bioactivity. To overcome the limitations of the raw ESM, we proposed new ESM-based functional platforms using nanomaterial for the various applications in the fields of healthcare (e.g., bone tissue engineering). Firstly, we fabricated the ESM solution by dissolving the disulfide bond of the raw ESM for the fabrication of ESM-based platforms with various size. Secondly, we used hydroxyapatite powders (HAp) with nanoscale that can be incorporated into the ESM solution to enhance the mechanical and bioactive property of the raw ESM. Finally, we confirmed that the proliferation and differentiation of the osteoblast-like cells were increased on the HAp/ESM composite with appropriate composite conditions compared to those on the ESM solution. The proposed HAp/ESM composite could be used as new biomaterials for healthcare applications including bone tissue engineering.

PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs

Kim, Yoosik,Park, Joha,Kim, Sujin,Kim, MinA,Kang, Myeong-Gyun,Kwak, Chulhwan,Kang, Minjeong,Kim, Baekgyu,Rhee, Hyun-Woo,Kim, V. Narry Elsevier 2018 Molecular cell Vol.71 No.6

<P><B>Summary</B></P> <P>Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find that various noncoding RNAs interact with PKR. Surprisingly, the majority of the PKR-interacting RNA repertoire is occupied by mitochondrial RNAs (mtRNAs). MtRNAs can form intermolecular dsRNAs owing to bidirectional transcription of the mitochondrial genome and regulate PKR and eIF2α phosphorylation to control cell signaling and translation. Moreover, PKR activation by mtRNAs is counteracted by PKR phosphatases, disruption of which causes apoptosis from PKR overactivation even in uninfected cells. Our work unveils dynamic regulation of PKR even without infection and establishes PKR as a sensor for nuclear and mitochondrial signaling cues in regulating cellular metabolism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> fCLIP-seq reveals PKR-interacting endogenously expressed dsRNAs </LI> <LI> PKR binds to various noncoding RNAs such as retrotransposons and satellite RNAs </LI> <LI> MtRNAs can form intermolecular dsRNAs and strongly interact with PKR </LI> <LI> MtRNAs can regulate PKR phosphorylation and signaling, especially under stress </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hydroxyapatite/Eggshell Membrane Composites for Healthcare Applications

( Sujin Kim ),( Sunho Park ),( Daun Kim ),( Woochan Kim ),( Sungmin Park ),( Jangho Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.2

Eggshell membrane (ESM), one of the agricultural bioresources, is being recognized as emerging engineering platforms in various fields including healthcare applications. ESM is the protein-rich membrane between the eggshell and egg white, having interesting characteristics such as microfibrous structures and many functional chemical groups. However, the raw ESM has many limitations (e.g., limited scale-up platforms, low mechanical and bioactivity property), which raised some problems for its wide use as healthcare platforms. Here, we present new ESM-based engineering platforms based on nanotechnology for various healthcare applications, especially for bone tissue engineering (i.e., bone tissue repair and regeneration in animal and human systems). To enhance the mechanical and bioactivity property of law ESM, we used nanoscale hydroxyapatite powders (HAp) that can be incorporated into the ESM-based thin films. We demonstrated that the HAp/ESM composites provided unique properties of both HAp and ESM such as structural, chemical, and physical properties. Using the HAp/ESM composites, we showed that cell proliferation and differentiation of osteoblastic cells (MG-63) were enhanced on the HAp/ESM composites compared to those on the only ESM films. Our approach can enable the design and manipulation of ESM-based functional engineering platforms for healthcare applications including bone tissue engineering.

Associations of Polyp Characteristics in Children and Adolescents Presenting with Less Than Five Colorectal Polyps: A Full Colonoscopy Is Still Required

Kim Ju Young,Kim Yu Bin,Choi Sujin,Lee Yoo Min,Kim Hyun Jin,Kim Soon Chul,Jang Hyo-Jeong,Choi So Yoon,Yi Dae Yong,Lee Yoon,Choi You Jin,Kang Yunkoo,Lee Kyung Jae,Suk Jin Hong,Hwang Jun Hyun,Kwak Sangg 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.3

Background/Aims: A full colonoscopy is currently required in children and adolescents with colorectal polyps, because of their potential of neoplastic transformation and complications such as intussusception. We aimed to analyze the associations of polyp characteristics in children and adolescents with colorectal polyps. Based on these findings, we also aimed to reevaluate the necessity of conducting a full colonoscopy. Methods: Pediatric patients <18 years of age who had undergone a colonoscopic polypectomy and those with <5 colorectal polyps were included in this multicenter, retrospective study. Baseline clinicodemographics, colonoscopic and histologic findings were investigated. Results: A total of 91 patients were included. Multivariate logistic regression analysis showed that polyp size was the only factor associated with the presence of any polyps located proximal to the splenic flexure (odds ratio [OR], 2.25; 95% confidence interval [CI], 1.28 to 4.28; p=0.007). Furthermore, polyp location proximal to the splenic flexure and sessile morphology were associated with the presence of any adenomatous polyp (OR, 8.51; 95% CI, 1.43 to 68.65; p=0.023; OR, 18.41; 95% CI, 3.45 to 173.81; p=0.002, respectively). Conclusions: In children and adolescents presenting with <5 colorectal polyps, polyp size and the presence of any adenomatous polyp were positively associated with polyp location proximal to the splenic flexure. This finding supports the necessity of a full colonoscopic exam in pediatric patients with colorectal polyps for the detection of polyps before the occurrence of complications such as intussusception or neoplastic transformation.

Proteomic analysis for the effects of non-saponin fraction with rich polysaccharide from Korean Red Ginseng on Alzheimer's disease in a mouse model

Sujin Kim,Yunkwon Nam,Min-jeong Kim,Seung-hyun Kwon,Junhyeok Jeon,Soo Jung Shin,Soyoon Park,Sungjae Chang,Hyun Uk Kim,Yong Yook Lee,Hak Su Kim,Minho Moon The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Background: The most common type of dementia, Alzheimer's disease (AD), is marked by the formation of extracellular amyloid beta (Aβ) plaques. The impairments of axons and synapses appear in the process of Aβ plaques formation, and this damage could cause neurodegeneration. We previously reported that non-saponin fraction with rich polysaccharide (NFP) from Korean Red Ginseng (KRG) showed neuroprotective effects in AD. However, precise molecular mechanism of the therapeutic effects of NFP from KRG in AD still remains elusive. Methods: To investigate the therapeutic mechanisms of NFP from KRG on AD, we conducted proteomic analysis for frontal cortex from vehicle-treated wild-type, vehicle-treated 5XFAD mice, and NFP-treated 5XFAD mice by using nano-LC-ESI-MS/MS. Metabolic network analysis was additionally performed as the effects of NFP appeared to be associated with metabolism according to the proteome analysis. Results: Starting from 5,470 proteins, 2,636 proteins were selected for hierarchical clustering analysis, and finally 111 proteins were further selected for protein-protein interaction network analysis. A series of these analyses revealed that proteins associated with synapse and mitochondria might be linked to the therapeutic mechanism of NFP. Subsequent metabolic network analysis via genome-scale metabolic models that represent the three mouse groups showed that there were significant changes in metabolic fluxes of mitochondrial carnitine shuttle pathway and mitochondrial beta-oxidation of polyunsaturated fatty acids. Conclusion: Our results suggested that the therapeutic effects of NFP on AD were associated with synaptic- and mitochondrial-related pathways, and they provided targets for further rigorous studies on precise understanding of the molecular mechanism of NFP.

Source Investigation and Control of Ralstonia mannitolilytica Bacteremia in a Neonatal Intensive Care Unit: A Case Report

Kim Dabin,Kim Min Hye,Park Seul Gi,Choi Sujin,Lee Chan Jae,Jung Young Hwa,Choi Chang Won,Shin Myoung-Jin,Song Kyoung-Ho,Kim Eu Suk,Park Jeong Su,Kim Hong Bin,Lee Hyunju 대한소아감염학회 2023 Pediatric Infection and Vaccine Vol.30 No.1

A case of persistent Ralstonia mannitolilytica bacteremia in the neonatal intensive care unit prompted source investigation due to its rarity. After an extensive investigation, a contaminated ultrasonic nebulizer was identified as the source, and the infection was controlled by removing the source. This study emphasizes the importance of further investigations, even in single cases of rare pathogens.