Benzoate, m-Toluate, Phenol의 Pseudomonas putida에 의한 생분해

김장규,김석형,김태환,이대광,김남기 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

It this study, basic data were obtained for the treatment of industrial waste water which includes aromatic compounds. Substrate concentrations used were 100ppm, 500ppm, and 1,000pprn and initial pH's were pH6, pH7, and pH8 in order to obtain the optimum conditions for treating benzoate, m-toluate, and phenol by Pseudomonas putida(KCTC 1644). Durations were 20hrs for 100ppm, 40hrs for 500ppm, and 60hrs for 1,000ppm before the static growth of Pseudomonas putida. 100% of 100ppm benzoate(0.31g cell/1) was biodegraded at pH6, pH7, and pH8 before 20hrs, 52.8% of 500ppm at pH8 in 40hrs, and 27.9% of 1,000ppm at pH8 in 60hrs. The best initial pH was pH8 for the biodegradation and the growth of Pseudomonas putida in benzoate medium. For m-toluate, the best initial pH was also pH8. At this pH, 40.6% of 10Dppm(0.31 cell/1), 21.9% of 500ppm, and 14.1% of 1,000ppm m-toluate were biodegraded respectively in limited time. But for phenol, the best initial pH was pH7. At this point, 9.8% of 100ppm(0.218 cell/1), 7.1% of 500ppm, and 4.7% of 1,000ppm phenol were biodegraded respectively. Therefore, the best carbon source in this experiment was benzoate. Pseudomonas putida was also able to biodegrade m-cresol, p-hydroxybenzoic acid, and benzaldehyde.

Phage Ф FSV가 Lactobacillus casei의 증식에 미치는 영향

김경태,이정준,서인영,나석환,백영진 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.2

Phage Φ FSV가 유산종균의 배양에 미치는 영향을 조사하기 위하여 prophage Φ FSV를 보유한 L. casei YIT 9018 균주나 prophage cured strain인 L. casei HYM 1213 균주에 phage Φ FSV(Lac S21)나 wild type phage Φ J-1(Lac J-1)을 임의의 농도로 각각 감염시켜 이들 균주의 증식을 비교하였다. Lac S21 phage를 L. casei YIT 9018 균주에 감염시켰을 경우, 초기 감염농도가 6.0×10 exp(6) pfu/㎖ 미만인 시료에서는 균주의 성장 및 유산생성능이 정상적이었으며, 오히려 phage수는 감소하였다. 그러나 L. casei HYM 1213균주는 Lac S21 phage에도 민감하게 반응하여, 감염된 균주는 감염시킨 농도에 상관없이 모두 2일균에서 phage가 10^9∼10^10 pfu/㎖로 급격히 증가하였으며, 계속 배양함에도 phage는 감소되지 않고 6일균에서 10^7∼10^9 pfu/㎖로 유지되었고, 이로 인하여 유산균은 정상 발효를 못하였다. 따라서 phage Φ FSV는 모균주인 L. casei YIT 9018 균주에는 어떠한 영향도 미치지 못하고, 오히려 prophage cured strains에는 민감하게 작용하여 비정상 발효를 유도함을 알 수 있었다. 또한, Lac J-1 phage는 prophage Φ FSW를 보유한 L. casei YIT 9018 균주와 prophage cured strain인 L. casei HYM 1213 균주에 모두 민감하게 감염되어 phage의 농도가 급증하고, 이로 인해 균주의 이상발효를 야기시켰다. In order to study effect of the phage Φ FSV on the growth of lactic acid bacteria, Lactobacillus casei YIT 9018 (wild type strain with prophage) or L. casei HYM 1213 (prophage cured strain) was infected with various concentrations of phage Φ FSV (called Lac S21) or wild type virulent phage (called Lac J-1). When L. casei YIT 9018 was infected with Lac S21 under the concentration of 6.0×10 exp(6) pfu/㎖, the growth and lactic acid production of the strain were normal and the number of phages decreased. But L. casei HYM 1213 was susceptible to Lac S21. Regardless of the concentration of the phage infection, the number of phages increased rapidly into 10^9 to 10^10 pfu/㎖ at 2 day cultures and was maintained 10^7 to 10^9 pfu/㎖ phages until 6 day cultures. The lactic acid production of L_n casei HYM 1213 infected with Lac S21 was abnormal. Therefore, phage Φ FSV had an evil effect on growth of L. casei HYM 1213, but not L. casei YIT 9018. On the other hand Lac J-1 caused abnormal fermentation to either L. casei YIT 9018 or L. casei HYM 1213.

단백뇨를 보이는 사구체 질환 및 당뇨병성 신병증에서의 Lp(a)

권태환,김준홍,조성,김석재,김용림,조동규,백미영 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

Background: Recently there has been evidences that serum Lp(a), an independent risk factor to atherosclerotic cardiovascular diseases, were increased in proteinuric disorders such as nephrotic syndrome and diabetic nephropathy. Methods: We intended to search of altered concentrations of Lp(a) in proteinuric disorder measuring serum Lp(a) concentrations with ELISA in 44 glomerulonephritic patients(25 nephrotic syndrome(NS), 19 non-nephrotic range proteinuric glomerulonephritis(GN), 25 diabetic nephropathy patients(DN), and 31 healthy controls(HC). Also, we compared Lp(a) concentration between glomerulonephritis patients and diabetic nephropathy patients with proteinuria of similar degree. Results: 1) There were significantly increased levels of total choesterol, triglyceride, and LDL-cholesterol in Ns compared to GN, DN, HC. 2) There were significantly increased concentrations of serum Lp(a) in NS compared to HC, but no signiicant difference in serum Lp(a) among NS, GN, and DN. 3) There was no significant difference in serum Lp(a) concentrations between NS & DN with 24 hour urine protein greater than 3.0g. 4) There was no significant difference in serum Lp(a) concentration between GN with 24 hour urine protein greater than 0.5g and less than 1.5g and DN with proteinuria of simial degree. 5) In glomerulonephritis patients, there was negative correlation between serum Lp(a) concentration and serum albumin level but correlation with 24 hour urinary protein, total cholesterol, Ldl-cholesterol, and HDL-cholesterol was not shown. In diabetic nephropathy, there was no significant correlation among serum Lp(a) concentration and all parameters including serum albumin, 24 hour urinary protein, and other lipid profiles. Conclusion: The present study confirmed that patients with nephrotic syndrome of diverse etiologies have makedly increased plasma level of Lp(a), in conjunction with other lipid abnormalities. However, this study shows no difference in Lp(a) concentrations between diabetic nephropathy and glomerulonephritis with similar degree of proteinuria.

제어 입력에 시간 지연을 갖는 선형 시스템의 견실한 H∞ 제어기 설계

류석환,김홍락 대구대학교 과학기술연구소 1997 科學技術硏究 Vol.4 No.1

In this paper, a solution of the H∞ control problem for linear uncertain systems with input time delay is presented. An H∞ norm bounded condition is obtained as a sufficient condition for linear uncertain systems with input time delay. Based upon this sufficient condition, a robust H∞ controller design method which involves the solutions of linear matrix inequalities is developed. A numerical example is solved in order to illustrate the efficacy of the suggested design method.

Secondary central nervous system (CNS) involvement in patients with diffuse large B-cell lymphoma: a therapeutic dilemma

Kim, Seok Jin,Oh, Sung Yong,Kim, Jin Seok,Kim, Hawk,Lee, Gyeong-Won,Won, Jong Ho,Shin, Ho Jin,Yang, Deok Hwan,Choi, Chul Won,Park, Jinny,Kim, Won Seog,Suh, Cheolwon Springer-Verlag 2011 Annals of hematology Vol.90 No.5

악성 복수 및 간 전이를 동반한 췌장의 Mucinous Ductal Ectasia 1예

김미영,김명환,김봉석,신규석,변종훈,두창준,소군호,진교현,김서종,고정석 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.6

A 71-year-old man was admitted due to abdominal distension and periumbilical pain. He was diagnosed as having mucinous ductal ectasia (MDE) of the pancreas three months prior, but refused an operation. Three months later, an abdominal computed tomography revealed more dilated pancreatic duct, newly developed liver metastasis and ascites in comparison with previous findings. Fine-needle aspiration cytology of the cystic lesion in the pancreatic head was conducted and yielded adenocarcinoma. Also, an ascitic fluid cytology determined adenocarcinoma. This patient was diagnosed to be inoperable and received palliative chemotherapy and pain control. The patient expired 5 months after the initial diagnosis.

Characterization of Nutritional Composition, Antioxidative Capacity, and Sensory Attributes of <i>Seomae</i> Mugwort, a Native Korean Variety of <i>Artemisia argyi</i> H. Lév. & Vaniot

Kim, Jae Kyeom,Shin, Eui-Cheol,Lim, Ho-Jeong,Choi, Soo Jung,Kim, Cho Rong,Suh, Soo Hwan,Kim, Chang-Ju,Park, Gwi Gun,Park, Cheung-Seog,Kim, Hye Kyung,Choi, Jong Hun,Song, Sang-Wook,Shin, Dong-Hoon Hindawi Publishing Corporation 2015 Journal of analytical methods in chemistry Vol.2015 No.-

<P>Few studies have investigated <I>Seomae</I> mugwort (a Korean native mugwort variety of <I>Artemisia argyi</I> H. Lév. & Vaniot), exclusively cultivated in the southern Korean peninsula, and the possibility of its use as a food resource. In the present study, we compared the nutritional and chemical properties as well as sensory attributes of <I>Seomae</I> mugwort and the commonly consumed species <I>Artemisia princeps</I> Pamp. In comparison with <I>A. princeps, Seomae</I> mugwort had higher contents of polyunsaturated fatty acids, total phenolic compounds, vitamin C, and essential amino acids. In addition, <I>Seomae</I> mugwort had better radical scavenging activity and more diverse volatile compounds than <I>A. princeps</I> as well as favorable sensory attributes when consumed as tea. Given that scant information is available regarding the <I>Seomae</I> mugwort and its biological, chemical, and sensory characteristics, the results herein may provide important characterization data for further industrial and research applications of this mugwort variety.</P>

An <i>H</i>–<i>T</i> diagram characterizing the activation barriers obtained from the magnetic relaxation of HgBa₂Ca₂Cu₃O_8+δ thin film

Kim, Myoung-Hwan,Lee, Sung-Ik,Kim, Mun-Seog,Kang, Won Nam IOP Publishing Ltd 2005 Superconductor science & technology Vol.18 No.6

<P>The magnetic relaxation of an epitaxial HgBa<SUB>2</SUB>Ca<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>8+δ</SUB> (Hg-1223) thin film was measured at several fields and temperatures. After the activation energy and the normalized relaxation rate had been analysed, the <I>U</I>–<I>j</I> characteristics were mapped on the <I>H</I>–<I>T</I> diagram. The power-law region with a creep exponent of <img SRC='http://ej.iop.org/images/0953-2048/18/6/008/sust197655ieqn1.gif' ALIGN='MIDDLE' ALT='\mu \simeq 7/9 '/>, which indicates large bundle creep, became a logarithmic region in the small μ limit as <I>j</I> went far below <I>j</I><SUB>c</SUB>. From this analysis, we found that a large bundle of flux acts like a single vortex, the size of which was temperature independent. This behaviour is quite different from that of other cuprate superconductors. Also, the crossover temperature, <I>T</I><SUB>b</SUB>, between the power-law and the logarithmic regions was inversely proportional to <I>H</I><SUP>β</SUP>. The fitting of <I>T</I><SUB>b</SUB>(β) on the <I>H</I>–<I>T</I> diagram is in good agreement with the field exponent of <img SRC='http://ej.iop.org/images/0953-2048/18/6/008/sust197655ieqn2.gif' ALIGN='MIDDLE' ALT='\beta \simeq 0.4 '/> that was obtained from the scaling of <I>U</I><SUB>eff</SUB>(<I>H</I>).</P>

Evaluation of the Efficacy of Kochiae fructus Extract in the Alleviation of Carbon Tetrachloride-induced Hepatotoxicity in Rats

Kim Na-Young,Lee Jeong-Sook,Kim Seog-Ji,Park Myoung-Ju,Kim Seok-Hwan The Korean Nutrition Society 2005 Nutritional Sciences Vol.8 No.4

Hepatoprotective effects of the extract of Kochiae fructus (KF), a traditional oriental medicinal plant, were evaluated against carbon tetrachloride($CCl_4$)-induced liver damage in rats. Male Sprague-Dawley rats were divided into control, $CCl_4,\;CCl_4$ plus methanol extract of KF (KFM-$CCl_4$), and $CCl_4$ plus butanol extract of KF (KFB-$CCl_4$) groups. KFM and KFB were orally administered once a day (200 mg/kg body weight) for 14 days. A mixture of 0.2 mL/100 g body weight of $CCl_4$ in olive oil was injected at 30 minutes after the final administration of KFM and KFB. The KFB pretreatment resulted in a significant decrease in the serum transaminase and lactic dehydrogenase levels in the $CCl_4$-treated rats. The $CCl_4$ treatment significantly lowered the activities of glutathione, glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px). However, pretreatment with KFM and KFB resulted in a significant increase in the glutathione, GR and GST levels. KFB increased the activities of SOD, catalase and GSH-Px, but KFM did not alter them. Pretreatment with KFM and KFB resulted in a significant decrease in the production of aminopyrine N-demethylase in the $CCl_4$-treated rats. KF extract would appear to contribute to alleviate the adveISe effect of $CCl_4$ treatment by enhancing the hepatic antioxidant defense system.

Influence of Geijibokryunghwan on Platelet Aggregation, Cyclic AMP, Cyclic GMP, TXA2, $Ca^{2+}$ Mobilization, Tyrosine Phosphorylation of PLC-$\gamma$2 and IP3 in Activated Platelets

Kim Han Geu,Kim Jong Gu,Kim Seog Ha,Sa Eun-Ho,Kim Jae-Woo,Moon Jin Young,Park Sun Dong,Choi Dall Yeong,Kim Cherl Ho,Park Won Hwan The Physiological Society of Korean Medicine and T 2004 동의생리병리학회지 Vol.18 No.6

Geijibokryunghwan has a wide range of therapeutic applications, and some reports have indicated that it has protective activity against atherosclerosis, and more specifically stroke and myocardial infarction. A recent report showed that atherosclerotic plaque volume can be reduced by supplying Geijibokryunghwan extracts for several years. In this study, we used a component of Geijibokryunghwan, which has been used for the prevention of atherosclerosis in Korea for several years, and has proven to be useful in lowering the occurrence of cerebral infarction. In a preliminary study, we found that Geijibokryunghwan potently suppressed platelet aggregation induced by various agonists. In this study, we sought to explore the mechanism by which Geijibokryunghwan inhibits platelet aggregations.