저장 온도 및 상대습도가 비타민 정제 중 지용성 비타민 함량의 변화에 미치는 영향

김동섭,이재황,김세곤,이동언,박석준,이진희,이강표,최성원,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

한국의 소집단 활동 진흥 방안에 관한 실증적 연구

구일섭,김태성,이강인 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

The purpose of this research is to suggest the policy making for the effective promotion of Quality Small-Group activity. Since 1975, variety of organizations in Korea have widely the implemented Quality Small-Group activities for their management systems. Today, Companies are facing the harsh realities of a competitive environment. This is no time for revolutionary change. Instead, Companies are instituting revolutionary change meant to have impact within a very short time frame. Bold steps are required to lead the industry into a future of improved efficiency and significant productivity gains. The Worker's Quality Small-Group activity is, essentially, a trouble shooting oriented voluntary small group within their working place to improve the surroundings creatively. The industries have enthusiastically used Quality Small-Group activities and were influenced from them. The Quality Small-Group activities can be regarded as a kind of Knowledge Management where new knowledge is created and knowledge is shared in the economy. However, the academics were less interested in this subject, as a result, there were no systematic guidances for Quality Circle activities. Also, the government was less interested in these activities, as a result, there were no systematic policy making for Quality Small-Group activities. Thus, in this research, the effective policy making for Quality Small-Group activities were suggested which were based on the results of survey Questionnaires. As a results of this research, to maximize the effect of TQM in the nationwide, Quality Small-Group activity as a bottom-up management should be more expanded and revitalized.

유아 로타바이러스 설사증에 대한 IgY 난황 항체의 효과시험

정동혁, 박효선, 김경미, 정규항, 김강영, 정후길, 윤숭섭, 전호남, 강신영 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

주요우울증에 대한 Milnacipran의 효과 및 안정성 : Fluoxetine과의 비교

이민수,함병주,기백석,김정범,연병길,오강섭,오병훈,이철,정한용,지익성,최병무,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This 6-week, open label randomized, multicenter study was conducted to evaluate the antidepressant effect and safety of milnacipran and fluoxetine in patients with major depression. Methods : The study was done in patients with major depression diagnosed by DSM-IV who score ≥17 in 17 items Hamilton Rating Scale for Depression (17-item HAM-D) and score ≥25 in Montgomery and Asberg Depression Rating Scale (MADRS). A total of 87 patients were randomized to milnacipran group and fluoxetine group. In cases of the patients taking other antidepressants, 6 weeks of each medication was administered after 7 days of drug excretion period. The evaluation was done using 17 item HAM-D, MADRS, Clinical Global Impression Scale (CGI), and COVI scale after baseline, 1 week, 2 weeks, 4 weeks, and 6 weeks. The side effects that had occurred during the period of our study were put in records by developed/disappeared time, severities, incidences, managements and results. Results : A total of 87 patients were enrolled. 70 (mitnacipran group 39 ; fluoxetine group 31) of them were included for the 6 weeks of research and 17 of them dropped out with in the first week, not due to adverse reactions or deficiency of effects. Total 17 item HAM-D scores, total points of MADRS, and CGI showed significant decrease after 1 week in each treatment group and continued decrease after 2 weeks and 4, 6 weeks. But there was no difference between milnacipran group and fluoxetine group in the antidepressant effect. There were no significant changes in vital sign, CBC, chemistry, and EKG in each treatment group. The commonly reported side elfects of minlacipran were nausea (25.0%), headache (10.7%), vomiting (7.1%),constipation (7.1%), dizziness (7.1%) and those of fluoxetne were GI trouble (11.1%), diarrhea (11.1%), insomnia (11.1%),agitation (5.6%), and dizziness (5.6%). Conclusion : Milnacipran was effective for the improvement of depressive symptoms and was well tolerated and safe in patients with depression.

The Preventive Effect of Topical Zafirlukast Instillation for Peri-Implant Capsule Formation in Rabbits

Kang, Shin Hyuk,Shin, Kee Cheol,Kim, Woo Seob,Bae, Tae Hui,Kim, Han Koo,Kim, Mi Kyung Korean Society of Plastic and Reconstructive Surge 2015 Archives of Plastic Surgery Vol.42 No.2

Background Capsular contracture is the most troublesome complication in breast implant surgery. Although capsule formation can be seen as a normal reaction to a foreign body, it can induce pain, hardness, deformity, and other pathologic problems. Surgical intervention is required in severe cases, but even surgery cannot guarantee a successful outcome without recurrence. This experimental study confirms that single topical administration of leukotriene antagonist zafirlukast (Accolate, Astrazeneca) reduces peri-implant capsule formation and prevents capsular contracture. Methods Twelve smooth-surfaced cohesive gel implants were implanted in New Zealand White rabbits. These miniature implants were designed to be identical to currently used products for breast augmentation. The rabbits were divided into 2 groups. In the experimental group (n=6), the implant and normal saline with zafirlukast were inserted in the submuscular pocket. In the control group (n=6), the implant and normal saline alone were used. Two months later, the implants with peri-implant capsule were excised. We evaluated capsule thickness and collagen pattern and performed immunohistochemical staining of myofibroblasts, transforming growth factor $(TGF)-{\beta}1$, 2. Results The thickness of the capsules in the experimental group was reduced in both dorsal and ventral directions. The collagen pattern showed parallel alignment with low density, and the number of myofibroblasts as well as the amounts of $TGF-{\beta}1$ and $TGF-{\beta}2$ were reduced in the experimental group. Conclusions We suggest that single topical administration of leukotriene antagonist zafirlukast can be helpful in reducing capsule formation and preventing capsular contracture via myofibroblast suppression, modulation of fibroblastic cytokines, and anti-inflammatory effect.

CO-Dependent H₂ Production by Genetically Engineered <i>Thermococcus onnurineus</i> NA1

Kim, Min-Sik,Bae, Seung Seob,Kim, Yun Jae,Kim, Tae Wan,Lim, Jae Kyu,Lee, Seong Hyuk,Choi, Ae Ran,Jeon, Jeong Ho,Lee, Jung-Hyun,Lee, Hyun Sook,Kang, Sung Gyun American Society for Microbiology 2013 Applied and environmental microbiology Vol.79 No.6

<P>Hydrogenogenic CO oxidation (CO + H<SUB>2</SUB>O → CO<SUB>2</SUB> + H<SUB>2</SUB>) has the potential for H<SUB>2</SUB> production as a clean renewable fuel. <I>Thermococcus onnurineus</I> NA1, which grows on CO and produces H<SUB>2</SUB>, has a unique gene cluster encoding the carbon monoxide dehydrogenase (CODH) and the hydrogenase. The gene cluster was identified as essential for carboxydotrophic hydrogenogenic metabolism by gene disruption and transcriptional analysis. To develop a strain producing high levels of H<SUB>2</SUB>, the gene cluster was placed under the control of a strong promoter. The resulting mutant, MC01, showed 30-fold-higher transcription of the mRNA encoding CODH, hydrogenase, and Na<SUP>+</SUP>/H<SUP>+</SUP> antiporter and a 1.8-fold-higher specific activity for CO-dependent H<SUB>2</SUB> production than did the wild-type strain. The H<SUB>2</SUB> production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild-type strain. The H<SUB>2</SUB> production rate of the engineered strain was severalfold higher than those of any other CO-dependent H<SUB>2</SUB>-producing prokaryotes studied to date. The engineered strain also possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H<SUB>2</SUB> production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.</P>

Metformin protects against retinal cell death in diabetic mice

Kim, Yoon Sook,Kim, Minjun,Choi, Mee Young,Lee, Dong Hoon,Roh, Gu Seob,Kim, Hyun Joon,Kang, Sang Soo,Cho, Gyeong Jae,Kim, Seong-Jae,Yoo, Ji-Myong,Choi, Wan Sung Elsevier 2017 Biochemical and biophysical research communication Vol.492 No.3

<P><B>Abstract</B></P> <P>Retinal degeneration is an early feature of diabetic retinopathy, the major cause of blindness in the developed world. Here we investigated how the widely used antidiabetic drug metformin reduces retinal injury in diabetic mice. Metformin was orally administered to control mice or mice with streptozotocin-induced diabetes. Western blot analysis showed that levels of O-linked β-<I>N</I>-acetylglucosamine (O-GlcNAc) transferase (OGT) and other related proteins such as carbohydrate-responsive element-binding protein (ChREBP) and thioredoxin-interacting protein (TXNIP) were significantly increased, and nuclear factor kappaB (NF-κB) and poly (ADP-ribose) polymerase (PARP) were activated in the diabetic retinas or retinal pigment epithelial (RPE) cells exposed to high glucose compared to controls. More importantly, RPE cells exposed to high glucose and treated with thiamet-G had higher levels of those proteins, demonstrating the role of elevated O-GlcNAcylation. Double immunofluorescence analysis revealed increased co-localization of terminal deoxynucleotide transferase-mediated dUTP nick-end labelling (TUNEL)-positive ganglion cells and OGT, ChREBP, TXNIP, or NF-κB in diabetic retinas compared to control retinas. Co-immunoprecipitation analysis showed that interaction between OGT and ChREBP or NF-κB was increased in diabetic retinas compared to control retinas, and this was accompanied by more cell death. Notably, metformin attenuated the increases in protein levels; reduced co-localization of TUNEL-positive ganglion cells and OGT, ChREBP, TXNIP, or NF-κB; and reduced interaction between OGT and ChREBP or NF-κB. Our results indicate that OGT inhibition might be one of the mechanisms by which metformin decreases retinal cell death.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Metformin decreases OGT, ChREBP, and TXNIP in the diabetic mouse retina. </LI> <LI> Metformin decreases NF-κB and PARP activation in diabetic mouse retinas. </LI> <LI> Metformin inhibits OGT interaction with ChREBP, decreasing retinal cell death. </LI> <LI> Metformin inhibits OGT interaction with NF-κB, decreasing retinal cell death. </LI> </UL> </P>

Noncancer-Related Health Events and Mortality in Head and Neck Cancer Patients After Definitive Radiotherapy : A Prospective Study

Kang, Ho-Seob,Roh, Jong-Lyel,Lee, Sang-wook,Kim, Sung-Bae,Choi, Seung-Ho,Nam, Soon Yuhl,Kim, Sang Yoon Williams & Wilkins Co 2016 Medicine Vol.95 No.19

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>The survival of patients with head and neck squamous cell carcinoma (HNSCC) can be affected not only by progression of the original cancer or occurrence of a second cancer but also by noncancer health event (NCHE). In this study, we evaluated the prognostic significance of early NCHEs in HNSCC patients after definitive radiotherapy (RT) or chemoradiotherapy (CRT).</P><P>The prospective study cohort comprised 190 HNSCC patients who underwent definitive RT (n = 75) or CRT (n = 115). An early NCHE was defined as an event requiring hospital readmission of the patient within 12 months after treatment. Univariate and multivariate analyses were performed to identify clinicopathologic factors associated with early NCHEs, and competing and all-cause mortalities.</P><P>Thirty-three patients suffered an NCHE (17.3%) and 8 succumbed to a competing cause of mortality (4.2%). Twenty-two (11.6%) patients had an early NCHE: respiratory (22.8%), cerebrovascular (13.7%), gastrointestinal (13.7%), and others (50.0%). In multivariate analysis, hypoalbuminemia (<I>P</I> = 0.022, hazard ratio [HR] = 3.66, 95% confidence interval [CI] = 1.21–11.1), chemotherapy (<I>P</I> = 0.047, HR = 3.02, 95% CI = 1.01–8.98), and tumor recurrence (<I>P</I> = 0.024, HR = 2.66, 95% CI = 1.14–6.22) were independent predictors of an early NCHE. Patients with early NCHEs were at high risk of competing mortality (<I>P</I> < 0.001, HR = 22.6, 95% CI = 4.21–121.00) and all-cause mortality (<I>P</I> = 0.002, HR = 4.44, 95% CI = 1.76–11.2).</P><P>Early NCHEs are a major contributor to competing and all-cause mortality in HNSCC patients receiving RT or CRT. The risk factors identified could be used to predict early NCHEs.</P></▼2>

Lateral Trapped-Charge Profiling Based on the Extraction of the Flatband Voltage by Using the Optical Substrate Current in Nitride-Based Charge-Trap Flash Memories

Kang-Seob Roh,Sungwook Park,Dae Hwan Kim,Dong Myong Kim IEEE 2009 IEEE transactions on electron devices Vol.56 No.9

<P>A lateral charge-profiling technique based on local-flatband-voltage (V<SUB>FB</SUB>)monitoring using optical-substrate-current spectroscopy (I<SUB>Sub,</SUB> <SUB>photo</SUB> spectroscopy) in nitride-based charge-trap flash (CTF) memories is proposed. Under optical illumination by photons with above-bandgap energy E<SUB>ph</SUB>(>E<SUB>g,Si</SUB>), I<SUB>Sub,</SUB> <SUB>photo</SUB> is abruptly increased at the gate voltage V<SUB>G</SUB>=V<SUB>FB</SUB> due to a sudden increase of the excess minority-carrier diffusion current. As expected in this principle, while the single-step feature of the I<SUB>Sub,</SUB> <SUB>photo</SUB>-V<SUB>G</SUB> curve is observed in the case of N-MOSFETs, a multistep response is clearly observed in nitride-based CTF memories. The mechanism of steplike I<SUB>Sub,</SUB> <SUB>photo</SUB> spectroscopy is analyzed, supported by analytical models, and verified by comparison with TCAD simulation results. The results show that the height of the step corresponds to the lateral length L<SUB>TC</SUB> of the region, over which localized trapped charges are distributed, and its width to the density Q<SUB>nit</SUB>(x)=qN<SUB>nit</SUB>(x) [C/cm<SUP>2</SUP>] in the nitride storage layer. Based on the proposed I<SUB>Sub,</SUB> <SUB>photo</SUB> spectroscopy, lateral charge profiling is demonstrated in a programmed NROM cell. The validity of the proposed I<SUB>Sub,</SUB> <SUB>photo</SUB> spectroscopy is confirmed by comparing the measured I<SUB>D</SUB>-V<SUB>G</SUB> characteristics with TCAD simulation incorporating the extracted N<SUB>nit</SUB>(x) by I<SUB>Sub,</SUB> <SUB>photo</SUB> spectroscopy. The proposed lateral charge-profiling technique is expected to be a useful technique for extracting the trapped-charge distribution in NROM and/or multibit CTF memories. This extraction technique has advantages of electrical stress free, exclusion of the effect from interface traps, and the applicability to devices with large gate leakage current.</P>

Caloric restriction improves diabetes-induced cognitive deficits by attenuating neurogranin-associated calcium signaling in high-fat diet-fed mice

Kim, Hwajin,Kang, Heeyoung,Heo, Rok Won,Jeon, Byeong Tak,Yi, Chin-ok,Shin, Hyun Joo,Kim, Jeonghyun,Jeong, Seon-Yong,Kwak, Woori,Kim, Won-Ho,Kang, Sang Soo,Roh, Gu Seob SAGE Publications 2016 Journal of cerebral blood flow and metabolism Vol.36 No.6

<P> Diabetes-induced cognitive decline has been recognized in human patients of type 2 diabetes mellitus and mouse model of obesity, but the underlying mechanisms or therapeutic targets are not clearly identified. We investigated the effect of caloric restriction on diabetes-induced memory deficits and searched a molecular mechanism of caloric restriction-mediated neuroprotection. C57BL/6 mice were fed a high-fat diet for 40 weeks and RNA-seq analysis was performed in the hippocampus of high-fat diet-fed mice. To investigate caloric restriction effect on differential expression of genes, mice were fed high-fat diet for 20 weeks and continued on high-fat diet or subjected to caloric restriction (2 g/day) for 12 weeks. High-fat diet-fed mice exhibited insulin resistance, glial activation, blood-brain barrier leakage, and memory deficits, in that we identified neurogranin, a down-regulated gene in high-fat diet-fed mice using RNA-seq analysis; neurogranin regulates Ca<SUP>2+</SUP>/calmodulin-dependent synaptic function. Caloric restriction increased insulin sensitivity, reduced high-fat diet-induced blood-brain barrier leakage and glial activation, and improved memory deficit. Furthermore, caloric restriction reversed high-fat diet-induced expression of neurogranin and the activation of Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase II and calpain as well as the downstream effectors. Our results suggest that neurogranin is an important factor of high-fat diet-induced memory deficits on which caloric restriction has a therapeutic effect by regulating neurogranin-associated calcium signaling. </P>