IgA 신병증 환자에서 Angiotensin Converting Enzyme 과 Endothelial Nitric Oxide Synthase유전자 다형성

김원,김현철,박성배,김인희,이광영,박성광,강성귀,고규영,이대열 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.3

To evaluate the association between ACE gene I/D polymorphism and ecNOS gene a/b polymorphism in IgA nephropathy, 158 IgA nephropathy patients and 121 control subjects were examined. In genotype distribution of the ACE gene I/D polymorphism, there was no significant difference in genotype distribution between controls and IgA nephropathy patients. We also examined the association between ACE genotype and clinical characteristics in the patients with IgA nephropathy. The incidence of hypertension in patients with DD genotype was higher than that of other genotypes. There were no significant association between I/D polymorphism distribution and chronic renal failure, nephrotic range proteinuria, and glomerular sclerosis in IgA nephropathy. In genotype distribution of ecNOS gene a/b polymorphism, there was no significant difference between IgA nephropathy patients and controls. There was no significant difference in frequency of chronic renal failure, hypertension, nephrotic range proteinuria and glomerular sclerosis among ecNOS genotypes. In addition, we failed to detect any significant association between the ACE and ecNOS gene-polymorphis ms and the decline of renal function in IgA nephropathy. A further study with larger number of patient population would be necessary.

Expression of Raf-1 Kinase Inhibitory Protein in Extrahepatic Bile Duct Carcinoma

Kim, Hyun-Soo,Kim, Gou Young,Lim, Sung-Jig,Kim, Youn Wha 대한병리학회 2010 Journal of Pathology and Translational Medicine Vol.44 No.3

Hydrogen Peroxide Produced by Angiopoietin-1 Mediates Angiogenesis

Kim, Young Mee,Kim, Kyung Eun,Koh, Gou Young,Ho, Ye-Shih,Lee, Kong-Joo American Association for Cancer Research 2006 Cancer research Vol.66 No.12

<P>Angiopoietin-1 (Ang1) mediates angiogenesis by enhancing endothelial cell survival and migration. It is also known that Ang1 activates Tie2, an endothelial-specific tyrosine kinase receptor, but the molecular mechanism of this process is not clear. In this study, we investigated whether reactive oxygen species (ROS) production plays a role in Ang1-mediated angiogenesis. We found that human umbilical vein endothelial cells treated with Ang1 produce ROS transiently, which was suppressed by NADPH oxidase inhibitor, diphenylene-iodonium chloride, and rotenone. The Ang1-induced ROS was identified as hydrogen peroxide (H2O2) using adenovirus-catalase infection. Removal of H2O2 by adenovirus-catalase significantly suppressed Ang1-induced in vitro endothelial cell migration, in vivo tubule formation and angiogenesis, and activation of p44/42 mitogen-activated protein kinase (MAPK), involved in cell migration, and delayed the deactivation of Akt phosphorylation involved in cell survival. Supporting to in vitro data, Ang1-induced vascular remodeling in catalase (-/-) mice was more prominent than in catalase (+/+) mice: Ang1-induced increases of the diameter of terminal arterioles and the postcapillary venules in catalase (-/-) mice were significant compared with catalase (+/+) mice. These results show that Ang1-induced H2O2 plays an important role in Ang1-mediated angiogenesis by modulating p44/42 MAPK activity.</P>

Imidazole-based alkaloid derivative LCB54-0009 suppresses ocular angiogenesis and lymphangiogenesis in models of experimental retinopathy and corneal neovascularization : Anti-angiogenic and anti-lymphangiogenic activities of LCB54-0009

Kim, Byung-Hak,Lee, Junyeop,Choi, Jun-Sub,Park, Dae Young,Song, Ho Young,Park, Tae Kyo,Cho, Chung-Hyun,Ye, Sang-Kyu,Joo, Choun-Ki,Koh, Gou Young,Kim, Tae-Yoon Wiley (Blackwell Publishing) 2015 British journal of pharmacology Vol.172 No.15

SoxF Transcription Factors Are Positive Feedback Regulators of VEGF Signaling

Kim, Kangsan,Kim, Il-Kug,Yang, Jee Myung,Lee, Eunhyeong,Koh, Bong Ihn,Song, Sukhyun,Park, Junseong,Lee, Sungsu,Choi, Chulhee,Kim, Jin Woo,Kubota, Yoshiaki,Koh, Gou Young,Kim, Injune Grune & Stratton 2016 Circulation research Vol.119 No.7

<P>Rationale: Vascular endothelial growth factor (VEGF) signaling is a key pathway for angiogenesis and requires highly coordinated regulation. Although the Notch pathway-mediated suppression of excessive VEGF activity via negative feedback is well known, the positive feedback control for augmenting VEGF signaling remains poorly understood. Transcription factor Sox17 is indispensable for angiogenesis, but its association with VEGF signaling is largely unknown. The contribution of other Sox members to angiogenesis also remains to be determined. Objective: To reveal the genetic interaction of Sox7, another Sox member, with Sox17 in developmental angiogenesis and their functional relationship with VEGF signaling. Methods and Results: Sox7 is expressed specifically in endothelial cells and its global and endothelial-specific deletion resulted in embryonic lethality with severely impaired angiogenesis in mice, substantially overlapping with Sox17 in both expression and function. Interestingly, compound heterozygosity for Sox7 and Sox17 phenocopied vascular defects of Sox7 or Sox17 homozygous knockout, indicating that the genetic cooperation of Sox7 and Sox17 is sensitive to their combined gene dosage. VEGF signaling upregulated both Sox7 and Sox17 expression in angiogenesis via mTOR pathway. Furthermore, Sox7 and Sox17 promoted VEGFR2 (VEGF receptor 2) expression in angiogenic vessels, suggesting a positive feedback loop between VEGF signaling and SoxF. Conclusions: Our findings demonstrate that SoxF transcription factors are indispensable players in developmental angiogenesis by acting as positive feedback regulators of VEGF signaling.</P>

Systemic Analysis of Tyrosine Phosphorylated Proteins in Angiopoietin-1 Induced Signaling Pathway of Endothelial Cells

Kim, Young Mee,Seo, Jawon,Kim, Yung Hee,Jeong, Jaeho,Joo, Hye Joon,Lee, Dong-Hee,Koh, Gou Young,Lee, Kong-Joo 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.18

Angiogenesis is an essential process in physiological and pathological processes and is well-regulated to maintain the cellular homeostasis by balancing the endothelial cells in proliferation and apoptosis. Angiopoietin-1 (Ang1) regulates angiogenesis as a ligand of Tie 2 receptor tyrosine kinase. however, the regulation pathways are not well-understood. To date, only a few of the signaling molecules involved in the Tie 2 receptor tyrosine kinase-mddiated angiogenesis have been identified. In this study, we systematically identified tyrosine-phosphorylated proteins in Ang1-induced signaling cascade in human umbllical vein endothelial cells (HUVECs), employing proteomic analyses combining two-dimensional gel electrophoresis, Western analysis using phosphotyrosine antibody and mass spectrometry (MALDI TOF MS and nanoLC-ESI-q-TOF tandem MS). We report here the identification, semiquantitative analysis, and kinetic changes of tyrosine-phosphorylated proteins in response to Ang1 in HUVECs and identified 66 proteins among 69 protein spots showing significant changes. Of these, P54nrb was validated as a molecule involved in cell migration. These results suggest that Ang1 induces stabilization of neo-vessel network by regulating thel phosphorylations of metabolic and structural proteins.

Giant superficial angiomyxoma of the vulva: a case report and review of the literature

Kim, Hyun-Soo,Kim, Gou Young,Lim, Sung-Jig,Ki, Kyung-Do,Kim, Hyun Cheol Blackwell Publishing Ltd 2010 Journal of cutaneous pathology Vol.37 No.6

<P>Superficial angiomyxomas (SAMs) are rare, benign cutaneous tumors frequently involving the subcutis. Only 15 cases of SAM involving the vulva have been reported, ranging from 0.9 to 4 cm in diameter. A 26-year-old woman presented with a 7-year history of a large, pedunculated cutaneous mass on the left labium major, measuring 12.5 × 11 × 10.5 cm and mimicking a soft tissue sarcoma. The mass was relatively well-circumscribed, but unencapsulated and multilobulated. Microscopically, the mass showed a conglomerate of moderately-to-sparsely cellular angiomyxoid lobules. Each lobule consisted of scattered spindle-shaped or stellate tumor cells set in an abundant myxoid stroma. Thin-walled, small-to-medium-sized blood vessels were distributed diffusely throughout the stroma. Scattered stromal neutrophils were also observed. No large vessels or plexiform capillaries were apparent. There was no perivascular accentuation of stromal cells or smooth muscle bundles. The tumor cells constantly expressed vimentin, CD34, CD44 and S-100, but none expressed estrogen receptors (ERs) and progesterone receptors (PRs), desmin or cytokeratin. Together, these findings were diagnostic of a SAM. Giant SAMs of the vulva can mimic aggressive angiomyxomas (AAMs) and angiomyofibroblastomas (AMB), as well as soft tissue sarcomas. Giant SAMs should be included in the differential diagnosis of vulvar soft tissue tumors.</P><P>Kim H-S, Kim GY, Lim S-J, Ki K-D, Kim HC. Giant superficial angiomyxoma of the vulva: a case report and review of the literature.</P>

Hydrogen Peroxide Produced by Angiopoietin-1 Mediates Angiogenesis

Kim, Young Mee,Kim, Kyung Eun,Koh, Gou Young,Ho, Ye-Shih,Lee, Kong-Joo 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.17

Angiopoietin-1 (Ang1) mediates angiogenesis by enhancing endothelial cell survival and migration, It is also known that Ang1 activates Tie2, an endothelial-specific tyrosine kinase receptor, but the molecular mechanism of this process is not clear, In this study, we investigated whether reactive oxygen species (ROS) Production plays a role in Ang1-mediated angiogenesis. We found that human umbilical vein endothelial cells treated with Ang1 produce ROS transiently, which was suppressed by NADPH oxidase inhibitor, diphenylene-iodonium chloride, and rotenone. The Ang1-induced ROS was identified as hydrogen peroxide (H_(2)O(2)) using adenovirus-catalase infection. Removal of H_(2)O_(2) by adenovirus-catalase significantly suppressed Ang1-induced in vitro endothelial cell migration, in viva tubule formation and angiogenesis, and activation of p44/42 mitogen-activated protein kinase (MAPK), involved in cell migration, and delayed the deactivation of Akt phosphorylation involved in cell survival. Supporting to in vitro data, Ang1-induced vascular remodeling in catalase (-/-) mice was more prominent than in catalase (+/+) mice: Ang1-induced increases of the diameter of terminal arterioles and the postcapillary venules in catalase (-/-) mice were significant compared with catalase (+/+) mice. These results show that Ang1-induced H_(2)O_(2) Plays an important role in Ang1-mediated angiogenesis by modulating p44/42 MAPK activity. (Cancer Res 2006; 66(12); 6167-74)

Stromal CD10 expression and relationship to the E-cadherin/&bgr;-catenin complex in breast carcinoma

Kim, Hyun-Soo,Kim, Gou Young,Kim, Youn Wha,Park, Yong-Koo,Song, Jeong-Yoon,Lim, Sung-Jig Blackwell Publishing Ltd 2010 Histopathology Vol.56 No.6

<P>Kim H-S, Kim G Y, Kim Y W, Park Y-K, Song J-Y & Lim S-J(2010) <I>Histopathology</I> <B>56,</B> 708–719Stromal CD10 expression and relationship to the E-cadherin/&bgr;-catenin complex in breast carcinoma</P><P>Aims: </P><P>Previous investigations have indicated that stromal CD10 expression, and altered levels of both E-cadherin and &bgr;-catenin, are associated with the biological aggressiveness of human carcinoma. The aim was to evaluate stromal CD10 expression and the association of stromal CD10 with E-cadherin and &bgr;-catenin in breast carcinoma.</P><P>Methods and results: </P><P>The expression of CD10, E-cadherin and &bgr;-catenin was immunohistochemically analysed in tissue microarrays containing 104 cases of invasive ductal carcinoma (IDC) and 10 cases of ductal carcinoma <I>in situ</I> (DCIS). Stromal CD10 was detected in 49.5% (50/101) of the IDC. No immunoreactivity was identified in the stromal cells of normal breast, DCIS or intraductal components of IDC. Accumulation of the cytoplasmic &bgr;-catenin was found in 87.0% (87/100) of the IDC. Stromal CD10 expression in IDC was significantly correlated with tumour size (<I>P</I> = 0.027), stage (<I>P</I> < 0.001) and histological grade (<I>P</I> = 0.006), the presence of nodal (<I>P</I> = 0.048) and distant (<I>P</I> = 0.015) metastases, oestrogen receptor-negative status (<I>P</I> = 0.016), cytoplasmic &bgr;-catenin accumulation (<I>P</I> = 0.031) and lower overall survival rate (<I>P</I> = 0.041).</P><P>Conclusions: </P><P>Stromal CD10 expression in IDC may constitute an important prognostic marker. Stromal CD10 expression with associated aggressive features might be related to aberrant &bgr;-catenin expression.</P>

Sixteen Cases of Sclerosing Hemangioma of the Lung Including Unusual Presentations

Kim Gou Young,김진국,최용수,김호중,Ahn Geunghwan,한정호 대한의학회 2004 Journal of Korean medical science Vol.19 No.3

Sclerosing hemangiomas (SH) of the lung are uncommon tumors and are thought to be benign. However, the biologic behavior of this tumor has not yet been characterized adequately. The clinicopathologic features were reviewed and analyzed for 16 cases of SH. The age of the patients ranged from 37 to 73 yr (mean 50.6 yr). There were fifteen female and one male patient. The SH located at the intraparenchyme in 14 cases, the interlobar fissure in one case and the visceral pleura in one case. The size of SH ranged from 0.3 cm to 8 cm (mean 2.6 cm). There were five unusual presentations of SH including a case having two SH with multiple nodules of atypical adenomatous hyperplasia in the same lobe, a case showing adenocarcinoma-like area within the SH, a case showing one peribronchial lymph node metastasis (N1 nodal stage) with location of interlobar major fissure, a case showing alveolar adenoma-like area within the SH, and one case with a large visceral pleural-based pedunculated mass presenting as mediastinal mass. All patients were alive and well without recurrence at the last follow up. Here, we reviewed previously published literatures and discussed the histogenesis of SH.